• Biomolecules & Therapeutics
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• v.4 no.1
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• pp.103-109
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• 1996

To investigate the effect of the third intracellular loop (i3 loop) peptide of human $\beta$$_2$-adrenergic receptor on receptor agonist binding, we expressed third intracellular loop region of human $\beta$$_2$-adrenergic receptor as glutathione S-transferase fusion protein in E. coli. DNA fragment of the receptor gene which encodes amino acid 221-274 of human $\beta$$_2$-adrenergic receptor was amplified by polymerase chain reaction and subcloned into the bacterial fusion protein expression vector pGEX-CS and expressed as a form of glutathione-S-transferase (GST) fusion protein in E. coli DH5$\alpha$. The receptor fusion protein was identified by SDS-PAGE and Western blot using monoclonal anti-GST antibody. The fusion protein expressed in this study was purified to an apparent homogeneity by glutathione Sepharose CL-4B affinity chromatography. The purified i3 loop fusion proteins at a concentration of 10 $\mu\textrm{g}$/ι caused right shift of the isoproterenol competition curve of [$^3$H]Dihydroalprenolol binding to hamster lung $\beta$$_2$-adrenergic receptor indicating lowered affinity of isoproterenol to $\beta$$_2$-adrenergic receptor possibly due to the uncoupling of receptor and G protein in the presence of the fusion protein. The uncoupling of receptor and G protein suggests that i3 loop region plays a critical role on $\beta$$_2$-adrenergic receptor G protein coupling.

• Journal of KIISE:Computing Practices and Letters
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• v.6 no.2
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• pp.225-233
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• 2000

Local Number Portability (LNP), an Intelligent Network (IN) service, provides the ability to retain existing telephone numbers when switching from one telecommunications carrier to another, from one region to another, or from one service to another. Local Number Portability (LNP), an Intelligent Network (IN) service, provides the ability to retain existing telephone numbers when switching from one telecommunications carrier to another, from one region to another, or from one service to another. LNP is a key service for increasing competition in the local telephone marketplace. Many countries are now establishing detailed rules for ensuring competition between network operators. Because many major carriers will deploy their own LNP databases, a single access point must be provided to effectively manage and distribute updates to the common regional LNP database. This is called Number Portability Administration Center (NPAC). Recently, CORBA is being accepted as one of the key technologies for developing distributed application systems. To use CORBA for managing IN services, several standard organizations are working towards the integration of IN, TMN and CORBA. In this paper, we present our work on applying the TMN and CORBA technology for the service management of LNP. We first propose a CORBA-based LNP service management system architecture, We then present the design and implementation of a LNP service management system using CORBA.

• BMB Reports
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• v.29 no.2
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• pp.175-179
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• 1996

Viral surface proteins are known to play an essential role in attachment of the virus particle to the host cell membrane. In case of the hepatitis B virus (HBV) several reports have described potential receptors on the target cell side, but no definite receptor protein has been isolated yet. As for the viral side, it has been suggested that the preS region of the envelope protein, especially the preS1 region, is involved in binding of HBV to the host cell. In this study, preS1 region was recombinantly expressed in the form of a maltose binding protein (MBP) fusion protein and used to identify and visualize the expression of putative HBV receptor(s) on the host cell. Using laser scanned confocal microscopy and by FACS analysis, MBP-preS1 proteins were shown to bind to the human hepatoma cell line HepG2 in a receptor-ligand specific manner. The binding kinetic of MBP-preS1 to its cellular receptor was shown to be temperature and time dependent. In cells permeabilized with Triton X-100 and treated with the fusion protein, a specific staining of the nuclear membrane could be observed. To determine the precise location of the receptor binding site within the preS1 region, several short overlapping peptides from this region were synthesized and used in a competition assay. In this way the receptor binding epitope in preS1 was revealed to be amino acid residues 27 to 51, which is in agreement with previous reports. These results confirm the significance of the preS1 region in virus attachment in general, and suggest an internalization pathway mediated by direct attachment of the viral particle to the target cell membrane.

• Journal of Korea Port Economic Association
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• v.31 no.3
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• pp.93-105
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• 2015

The purpose of this study is to outline the strategic implications of a structure analysis and trends of transferred air cargoes for the main airports of the North Asian region for 13 years from 2000 to 2012. To do so, it analyses the concentration levels using a Gini coefficient and Hirschmann Herfindahl index and represents the competitive positioning with regard to the main East Asian airports using the BCG framework and shift-share analysis. Currently, the concentration level is getting weaker in response to the emergence of Chinese airports in the north-east region. It is likely that the steady growth of air cargo of Shanghai airport (PVG) has caused the increase in concentration levels in the region. Seoul airport (ICN) may be directly in competition with PVG for air cargo in the region, giving consideration to reducing or maintaining an average increase ratio of air cargoes of ICN, NRT (Tokyo), and KIX (Osaka) for same period. In the same sense, the average increase in ratio for ICN for same period yields 1.3%, which is far behind the 6.2% and 5.2% of the north-east region and PVG respectively. Additionally, shift-share effect analysis indicates that ICN lost 422,180 tons in the north-east region during the same period, suggesting that transferred cargoes might have been forwarded to PVG.

• Journal of the Korea Institute of Building Construction
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• v.21 no.4
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• pp.319-331
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• 2021

This study analyzed diversification and diversification indices by region and construction type of overseas construction in major global countries using the Berry-Herfindahl Index. The analysis found that advanced European countries account for most of the countries with a high overseas share of global design and construction companies, and a high degree of diversification by engineering and by region. Korea is pushing for diversification through the specialization of construction enterprises by type of construction, and design enterprises are concentrated in some areas and specializations by type of construction and region. In the future, Korea's global construction companies need to strengthen their overseas competitiveness by diversifying their overseas business areas and their industrial sectors in the way that advanced countries have.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.23 no.58
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• pp.41-47
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• 2000

Global(mega) event has increased throughout the 1990s, with the biggest surge occurring in the Asia-Pacific region. Along with this upward trend, competition for more desirable tourists is also surging, so destinations cannot be complacent (Getz, 1997). Event is appearing as the powerful method in the fierce competition around the industry. International events like 99 Hanam Environment Expo have been held annually in Korea since 1991 in which the local government system started. The main roles of event are to enhance the image of communities and attract tourists (Kotler and Haider and Rein, 1993), to strengthen destination attractiveness as drawing power (Mill and Morrison, 1985), and so on. Focused on the destination image, the research (Sirakaya and Sheppard and McLellan, 1997) about the effect of perceived safety at a potential vacation destination showed that destination marketers, travel agents, and hospitality industry members should be concerned with improving their images when such images are negative with regard to safety. Event industry in Korea does not seriously recognize the importance of the safety management against accidents known for tarnishing the destination images. Moreover few safety standards or studies for event industry can be found. This paper investigated the safety factors considered by visitors of 99 Hanam Environment Expo and analyzed the correlation between the safety factors and the demographic characteristics of the visitors.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.05a
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• pp.575-578
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• 2001

Number Portability means that one can use his original telephone number when he change telephone service provider, region and the kind of service. Number portability is not necessary when only one telephone service provider exist in korea. But it is necessary to guarantee pair competition among the service providers as new communication service provider comes. The law which is related number portability is established by government as number portability is considered a tool to guarantee pair competition between old service provider and new service provider. A national standard on number portability with which all service provider can agree is needed. We developed functional standard for that purpose. Functional standard includes call processing structure, network requirement. This paper handle functional standards.

• BMB Reports
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• v.43 no.12
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• pp.807-812
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• 2010

$NF{\kappa}B$ and ZAS3 are transcription factors that control important cellular processes including immunity, cell survival and apoptosis. Although both proteins bind the ${\kappa}B$-motif, they produce opposite physiological consequences; $NF{\kappa}B$ activates transcription, promotes cell growth and is often found to be constitutively expressed in cancer cells, while ZAS3 generally represses transcription, inhibits cell proliferation and is downregulated in some cancers. Here, we show that ZAS3 inhibits $NF{\kappa}B$-dependent transcription by competing with $NF{\kappa}B$ for the ${\kappa}B$-motif. Transient transfection studies show that N-terminal 645 amino acids is sufficient to repress transcription activated by $NF{\kappa}B$, and that the identical region also possesses intrinsic repression activity to inhibit basal transcription from a promoter. Finally, in vitro DNA-protein interaction analysis shows that ZAS3 is able to displace $NF{\kappa}B$ by competing with $NF{\kappa}B$ for the ${\kappa}B$-motif. It is conceivable that ZAS3 has therapeutic potential for controlling aberrant activation of $NF{\kappa}B$ in various diseases.

• Journal of Navigation and Port Research
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• v.46 no.5
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• pp.449-457
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• 2022

The Port of Busan is currently ranked as the seventh largest container port worldwide in terms of cargo throughput. However, port competition in the Far-East region is fierce. The growth rate of container throughput handled by the port of Busan has recently slowed down. In this study, we analyzed how economic conditions and multiple external shocks could influence cargo throughput and identified potential implications for port business. The aim of this study was to build a model to accurately forecast port throughput using the ARIMA model, which could incorporate external socio-economic shocks, and the VEC model considering causal variables having long-term effects on transshipment cargo. Findings of this study suggest that there are three main areas affecting container throughput in the port of Busan, namely the Russia-Ukraine war, the increased competition for transshipment cargo of Chinese ports, and the weaker growth rate of the Korean economy. Based on the forecast, in order for the Port of the Port of Busan to continue to grow as a logistics hub in Northeast-Asia, policy intervention is necessary to diversify the demand for transshipment cargo and maximize benefits of planned infrastructural investments.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.05a
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• pp.118-120
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• 2023

Increasing port competition driven by the containerisation has motivated ports and terminals to focus on their performance to efficiently utilise the available resources and to make strategic decisions in port development and expansion. With both inter-port andintra-port competition increasing in the port of Colombo, this study aims to measure the efficiency of the container terminals in Colombo comparing to terminals in the port of Busan using the DEA window analysis to determine their operational efficiency and to provide suggestions for future port development activities. Multiple window analyses were conducted using CCR and BCC models with different orientations and window lengths to compare the efficiencies of 11 DMUs in both ports during the period from 2015-2019 to measure the efficiencies prior to the COVID-19 pandemic. Results revealed the largest terminal operator, PNC in Busan, to be the most efficient overall, while the second highest efficiency was recorded by one of the smallest terminal operators, SAGT in Colombo, among the sample. Although use of DEA in port performance measurement has been popular for many years, efficiency measurements in the port of Colombo, the main hub port in the South Asian region, has not been comprehensively studied so far.