• Journal of Korean Society of Environmental Engineers
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• v.27 no.2
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• pp.198-205
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• 2005

This study was carried out to investigate the removal of TNT (2,4,6-trinitrotoluene) in the batch and continuous type microcosm systems consisting of marsh and pond. First, the batch reactor study showed that TNT (10 mg/L) was completely removed in the marsh and pond system within 20 days. The major reductive metabolites of TNT include 4-amino-2,6-dinitrotoluene (4-ADNT), 2-amino-4,6-dinitrotoluene (2-ADNT), 2,4-diamino-6-nitrotoluene (2,4-DANT), and 2,6-diamino-4-nitrotoluene (2,6-DANT). These metabolites concentration also decreased during further treatment. The continuous reactor systems combining marsh and pond indicated the similar pattern of TNT degadation and the metabolites production. Among the continuous reactor combinations, marsh-pond system showed more stable TNT removal and metabolites production. The toxicity of the effluent from the continuous system was examined by Microtox Assay using Vibrio fischeri. The result showed that the effluent toxicity was reduced below toxicity endpoint ($EC_{50}$) after continuous marsh pond system, indicating that metabolites of TNT are less toxic than TNT itself. Based on the results, TNT contaminated wastewater can be efficiently treated using marsh and pond wetland systems.

• Journal of the Korean Applied Science and Technology
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• v.33 no.1
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• pp.30-40
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• 2016

This study related to get skin improvement effect of sheet mask pack using 5% of $2^{nd}$ fermented snail extract. To the scientific study of the skin, it was used for this sheet mask of 100% cotton as cutting face shape. We reported the results of measurement of skin improvement effect with the placebo and Gene-SEM (general snail extract mask). First; moisturizing effect of 2F-SEM ($2^{nd}$ fermented snail extract mask) has been increased about 11% than the PM and Gene-SEM was improved about 4.7% more than the PM (placebo mask). Second; elasticity of the mask pack containing $2^{nd}$ fermented snail extract was 13.8% better than PM and Gene-SEM was improved about 6.7 % more than PM. Third; Skin roughness of 2F-SEM has improved 6.80% than PM and Gene-SEM was improved about 2.3% more than PM. Fourth; melanin reductive effect of 2F-SEM was improved about15.0% more than PM and Gene-SEM was improved about 8.7% more than PM. Fifth; fine wrinkle decline effect of 2F-SEM was about 8.0% better than PM and Gene-SEM was improved with 5.1% more than PM. Sixth; sensorial evaluation of 2F-SEM using $2^{nd}$ fermented snail extract was significantly showed difference merits regarding softness, moisture, fine-wrinkle improvement.

• Journal of the Korean Chemical Society
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• v.53 no.5
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• pp.530-535
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• 2009

Dicyclohexyl telluride was obtained in a high yield by the reaction of cyclohexyl bromide with NaTeH(prepared in situ) in an aqueous ethanolic solution. A series of new organotelluronium salts of the general formula ${(cyclo-C_6H_11)}_2Te(R)X$ (where R = $CH_3$, X = I (1); R = $C_2H_5$, X = Br(2); R = $C_2H_5$, X = I (3); R = C_3H_5$, X = Br (4)) were prepared by the reaction of ${(cyclo-C_6H_11)}_2Te$ with the corresponding alkyl halide. Reaction of 1 with NaBPh4 gave compound 5 ( i.e. R = CH3, X = BPh4 ‒) in 78% yield. Reaction of ${(cyclo-C_6H_11)}_2Te$ with benzyl bromide and 4-bromophenacyl bromide gave unexpectedly dibenzylcyclohexyltelluronium bromide (6) and bis(4-bromophenacyl)cyclohexyltelluronium bromide (7), respectively. Reaction of 6 with NaBPh4 gave the corresponding tetraphenylborate derivative (8) in high yield. $^1H$ NMR studies revealed that in $CDCl_3$solution compound 1 eliminated alkyl halide. Conductivity, IR, $^1H\;and\;^{13}C$ NMR and thermal data for the new compounds are presented and discussed.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.98-106
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• 1987

The effect of carbofuran (2, 3-Dihydro-2,2-dimethyl-benzofuran-7-ylmethyl carbamate) on rice growth was evaluated as a direct growth stimulant of rice. For this, several laboratory and field trials conducted from 1981 to 1986 at the Yeongnam Crop Experiment Station. Carbofuran solution affected the germination of rice seed. The growth of seminal roots was adversely affected by the increase of carbofuran concentrations while the length of single root became longer with the concentration increment up to 50 ppm. Carbofuran application (0.18g ai/$m^2$) at the rice nurserybed significantly enhanced the rice growth and recovered from the Low temperature damage. The enhancement effect was more pronounced at the plot that applied carbofuran before rice seeding as soil incorporation than top-dressing. The effect of growth enhancement further extended to transplanted lowland rice. This effect was greater at double cropping area (late of June transplanting) compared to single cropping area (May transplanting). Among important agronomic traits, the increment of panicle number was the most important direct effect for increasing rice grain yield by carbofuran application. Carbofuran application also exhibited the reducing effect against low temperature damage at reductive division stage and at rice heading stage and against submergence damage at booting stage through enhancement of fertile grain ratio, ripening ratio or photosynthetic activity.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.149-156
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• 1997

We have employed comparative RFL,P(Restriction Fragment Ixngth Pol~iniorphism) analysis and molecular phylogenetic techniques to investigate the diversity of uncultured microorganisms associated with the anaerobic PCP degradation in PCP-adapted enrichment cultures inoculated by samples from anaerobic cewage sludgc(Jangrim, Pusan) and leachate of landfill site(Kimhae). 16s rDNA cloncs were obtairted by PCR amplification of mixed population DNAs extracted directly from the nonactive and active stage ol each PCP-adapted culture. After three rounds of comparative RFLP analyses. two RFLP types. designated as Ala and Hld, were found prevalent and common in both active stage samples. Thc analysis of phylogenctic diversity bawd on the 5'-terminal 180 nt of sequences from whole clones of the Ala and Bld RFLP types showed close similarity among themselves. In case of Bld clones, 7XQ of them shared identical sequences. Thcse resuliq suggest that the clones of both RFLP types wcre originated from highly affiliated microorganisms which are e~iriched as a result of metabolic activity to PCP. The full-length 16s rRNA sequence of each representative clone from both RFLP types was determined. and an Ala clone w i n found to he related to Clo.strrdiurn ulfutzac~(Genk~ank No. Z69203) and a Bld clone to Thermobacteroides proteolyticus(Genbank No. X09335), with sequence similarities of 89%' and 97%. respectively.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• Journal of the Korean Society for Marine Environment & Energy
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• v.17 no.1
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• pp.1-7
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• 2014

This paper aims to explain the safety assessment and remediation mechanism of Granulated Coal Ash (GCA) as a material for the remediation of coastal sediments and to evaluate the improvement of the sediment in Kaita Bay, where GCA was applied. The concentrations of heavy metal contained in GCA and the dissolved amounts of heavy metal from GCA satisfied the criteria for soil and water pollution in Japan. The mechanisms on the remediation of coastal sediments using GCA is summarized as follows; (1) removal of phosphate and hydrogen sulfide (2) neutralization of acidic sediment (3) oxidation of reductive sediment (4) increase of water permeability (5) increase of soil strength (6) material for a base of seagrass. From the results obtained from the field experiment carried out in Kaita Bay, it was clarified that GCA is a promizing material for remediation of coastal sediment. This remediation technology can contribute to promote waste reduction in society and to decrease cost of coastal sediment remediation by applying GCA in other polluted coastal areas.

• Korean Journal of Environmental Agriculture
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• v.27 no.1
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• pp.86-91
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• 2008

Dicamba(3,6-dichloro-2-methoxybenzoic acid) is used to control for pre and post-emergence of annual and perennial broad-leaf weeds. It is very soluble in water and highly mobile, acidic herbicide. So it is easily moved and detected in groundwater. Zerovalent iron(ZVI) has been used for the reductive degradation of certain compounds through amination of nitro-substituted compounds and dechlorination of chloro-substituted compounds. In this study, we investigated the potential of ZVI for the oxidative degradation of dicamba in water. The degradation rate of dicamba by ZVI was more rapidly increased in pH 3.0 than pH 5.0 solution. The degradation percentage of dicamba was increased with increasing amount of ZVI from 0.05% to 1.0%(w/v) and reached above 90% within 3 hours of reaction. As a result of identification by GC-MS after derivatization with diazomethane, we obtained three degradation products of dicamba by ZVI. They were identified 4-hydroxy dicamba or 5-hydroxy dicamba, 4,5-dihydroxy dicamba and 3,6-dichloro-2-methoxyphenol. 4-Hydroxy dicamba or 5-hydroxy dicamba and 4,5-dihydroxy dicamba are hydroxylation products of dicamba. 3,6-dichloro-2-methoxyphenol is hydroxyl group substituted compound instead of carboxyl group in dicamba. We also confirmed the same degradation products of dicamba in the Fenton reaction which is one of oxidation processes using ferric sulfate and hydrogen peroxide. But we could not find out the dechlorinated degradation products of dicamba by ZVI.

• Journal of Soil and Groundwater Environment
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• v.16 no.1
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• pp.82-90
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• 2011

Reactive reductants of cement/Fe(II) systems in dechlorinating chlorinated hydrocarbons have not been identified. The previous studies showed that a hematite/CaO/Fe(II) system had TCE degradation characteristics similar to those of cement/Fe(II) systems with regard to degradation kinetics and that lime (CaO) plays an important role in enhancing the reactivity for TCE dechlorination. The current study shows identified the formation of gypsum ($CaSO_4$) in the hematite/CaO/$FeSO_4$ system through the XRD analysis. The amounts of the gypsum increased with increment of the initial CaO dose. However, when CaO in the hematite/CaO/$FeSO_4$ system was replaced with gypsum, TCE degradation was not observed. Ca-removed Portland cement extracts (CPCX) in combination with $FeSO_4(CPCX/FeSO_4)$ showed no TCE degradation. On the other hands, the Portland cement extracts (PCX) in the presence of $FeSO_4(PCX/FeSO_4)$ and CPCX/CaO/$FeSO_4$ systems degraded 0.2 mM TCE within 5 days, indicating that CaO also played an important role dechlorination reactions in the systems. The pseudo-first-order rate constants (k) of the CPCX/CaO/$FeSO_4$ systems were 0.20, 0.24, and 0.72 $day^{-1}$, when the CaO dosages were 25, 50 and 75 g/L, respectively. The XRD analyses showed identified the common peaks having the d-values of 3.02, 2.27, and 1.87 in the reaction systems that showed TCE degradation. However, it was not possible to clearly identify the crystalline minerals having the three peaks from the references in JCPDS cards. This study reveals that the reactive agents in the cement/Fe(II) and the hematite/Fe(II) systems are likely to be those containing CaO and Fe(II).

• The Journal of Korean Obstetrics and Gynecology
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• v.18 no.1
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• pp.81-93
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• 2005

Purpose : There are various oriental medicine therapy of treating Pelvic Inflammatory Disease(PID) in clinics. We made the PID mice model by injection Lipopolysaccharide (LPS) in vagina, and investigate anti-inflammatory effects of Hongdeung-Tang among oral medication, retention enema therapy and herbal-acupuncture treatment. Method : The ICR(20-30g) mice(♀) were used. To examine the occurrence of inflammation, LPS in different concentration was injected into the vagina of the mice, and White Blood Cell(WBC) in blood was counted. To examine anti-inflammatory effects, 6 mice were assigned to each of the normal group, the control group and the sample group. Hongdeung-Tang was medicated in oral and rectal with 1.0g/kg low dose and 3.0g/kg high dose and by herbal acupucture for 5 days, 2 days before LPS injection. After 3days from LPS injection, blood was collected from retro-orbital plexus, and WBC, Interleukin-6(IL-6), Tumor Necrosis Factor-${\alpha}$(TNF-${\alpha}$) in the blood was counted. Result : After LPS injection with each dose, WBC count showed significant increase depending on LPS concentration from $100{\mu}g/kg$, and it was maximized at 3 or 4 days after LPS injection. the Hongdeung-Tang treatment groups, The number of WBC was decreased significantly only in low dose and high dose oral medication, and IL-6 concentration showed significant decrease in oral and rectal medication as well as in herbal acupuncture treatment. TNF-${\alpha}$ concentration was decreased significantly in oral and rectal medication of low dose and high dose. herbal-acupuncture treatment group datas showed reductive tendency. Conclusion : Based on above results, we made the PID mice model by injection LPS in vagina, and demonstrated anti-inflammatory effect of Hongdeung-Tang of oral medication, retention enema therapy and herbal-acupuncture treatment.