Anti-inflammatory Effects of Hongdeung-Tang on Mice with LPS Injected in Vagina

홍등탕(紅藤湯)이 LPS 질내(膣內) 주입(注入) 생쥐에 미치는 항염증(抗炎症)효과

  • Seo, Ji-Young (Dept. of Gynecology, Oriental Hospital, College of Oriental Medicine, Kyungwon University) ;
  • Kim, Yoon-Sang (Dept. of Gynecology, InCheon Oriental Hospital, College of Oriental Medicine, Kyungwon University) ;
  • Yun, Jeong-Mun ;
  • Lee, Tae-Hui ;
  • Lim, Eun-Mee (Dept. of Gynecology, Oriental Hospital, College of Oriental Medicine, Kyungwon University)
  • 서지영 (경원대학교 한의과대학 부속한방병원 한방부인과교실) ;
  • 김윤상 (경원대학교 한의과대학 인천한방병원 한방부인과교실) ;
  • 윤정문 ;
  • 이태희 ;
  • 임은미 (경원대학교 한의과대학 부속한방병원 한방부인과교실)
  • Received : 2004.08.02
  • Accepted : 2004.08.07
  • Published : 2005.02.28

Abstract

Purpose : There are various oriental medicine therapy of treating Pelvic Inflammatory Disease(PID) in clinics. We made the PID mice model by injection Lipopolysaccharide (LPS) in vagina, and investigate anti-inflammatory effects of Hongdeung-Tang among oral medication, retention enema therapy and herbal-acupuncture treatment. Method : The ICR(20-30g) mice(♀) were used. To examine the occurrence of inflammation, LPS in different concentration was injected into the vagina of the mice, and White Blood Cell(WBC) in blood was counted. To examine anti-inflammatory effects, 6 mice were assigned to each of the normal group, the control group and the sample group. Hongdeung-Tang was medicated in oral and rectal with 1.0g/kg low dose and 3.0g/kg high dose and by herbal acupucture for 5 days, 2 days before LPS injection. After 3days from LPS injection, blood was collected from retro-orbital plexus, and WBC, Interleukin-6(IL-6), Tumor Necrosis Factor-${\alpha}$(TNF-${\alpha}$) in the blood was counted. Result : After LPS injection with each dose, WBC count showed significant increase depending on LPS concentration from $100{\mu}g/kg$, and it was maximized at 3 or 4 days after LPS injection. the Hongdeung-Tang treatment groups, The number of WBC was decreased significantly only in low dose and high dose oral medication, and IL-6 concentration showed significant decrease in oral and rectal medication as well as in herbal acupuncture treatment. TNF-${\alpha}$ concentration was decreased significantly in oral and rectal medication of low dose and high dose. herbal-acupuncture treatment group datas showed reductive tendency. Conclusion : Based on above results, we made the PID mice model by injection LPS in vagina, and demonstrated anti-inflammatory effect of Hongdeung-Tang of oral medication, retention enema therapy and herbal-acupuncture treatment.

Keywords

References

  1. 婦科專病 中醫臨床論治 司徒儀;楊家林
  2. 中醫雜誌 v.32 no.9 中藥保留灌腸臨床應用近況 孫冠蘭
  3. 대한한방부인과학회지 v.15 no.2 骨盤炎 치료를 위해 사용된 保留灌腸療法에 관한 文獻的 考察 조성희;진천식
  4. 대한한방부인과학회지 v.12 no.1 續發性月經痛을 韓藥內服 및 藥物保留灌腸法으로 治療한 治驗 2例 엄은석;임은미
  5. 대한한방부인과학회지 v.13 no.1 卵巢囊腫을 韓藥內服 및 藥物保留灌腸法으로 治療한 治驗 1例 엄은석;임은미
  6. 대한한방부인과학회지 v.15 no.3 慢性骨盤炎을 韓方治療한 治驗 一例(韓藥內服藥과 保留灌腸治療 중심으로)
  7. 陝西中醫 v.17 no.10 紅藤湯灌腸治療慢性盆腔炎121例 王秀琴
  8. 中國中醫獨特療法大全 구沛然
  9. 東醫臨床婦人科學 肖淑春
  10. 약침요법총론 대한약침학회
  11. 대한한방부인과학회지 v.10 no.1 慢性骨盤痛에 關한 文獻的 考察 李泰均
  12. 鍼灸學(上) 全國韓醫科大學 針灸經穴學室
  13. 한방부인과학회지 v.16 no.2 紅藤湯의 經口와 直腸投與의 抗炎症效果比較硏究 장성환;김윤상;임은미
  14. 한방부인과학회지 v.16 no.3 康寧湯의 經口와 直腸投與의 抗炎症 效果 比較硏究. 송미선 등
  15. 대한한방부인과학회지 v.9 no.1 대하환자의 약침치료에 대한 임상적 고찰
  16. 대한한방부인학회지 v.14 no.1 은화사간탕 약침의 효능에 관한 연구.
  17. 새임상 부인과학 최유덕
  18. Harrison's principles of internal medicine T.R Harrison's(et al.)
  19. 대한산부인과학회지 v.42 no.9 산부인과 영역에서 감염증에 대한 치료로서 Pazeron의 유효성과 안전성을 평가하기 위한 제 4상 임상시험.
  20. 대한산부인과학회지 v.32 no.1 부인과감염증에서 유나신과 세프라딘/겐타마이신의 치료효과에 대한 비교연구. 김재욱 등
  21. JAMA v.266 Self-re ported pelvic inflammatory disease in the united states,1988. Aral SG;Mosher WD;Cates W
  22. 四川中醫 v.17 no.10 紅藤湯治療盆腔炎40例 周雪琴
  23. 中藥學 顔正華 등
  24. 本草學 전국한의대학 본초학교실
  25. 鄕藥大事典 정보섭;신민교
  26. AM J Pathol v.93 The effects of bacterial endotoxins on host mediation systems. A review. Morrison DC;Ulevitch RJ
  27. New Engl J med v.3 no.28 Pathogenetic mechanisms of septic shock. Parrillo JE
  28. 병리학 이중달
  29. 병리학 대한병리학회
  30. Immuno Biology Third edition. v.9 Charles A. Janeway, Jr.