• Title/Summary/Keyword: Red mold rice

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Monascus ruber의 액체배양을 통한 색소 생산 및 고체발효를 통한 홍국쌀의 monacolin K 생산 특성 (Production of Pigment by Liquid Culture and Monacolin K in Red Mold Rice by Solid State Fermentation of Monascus ruber Strains)

  • 박윤제
    • KSBB Journal
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    • 제28권6호
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    • pp.400-407
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    • 2013
  • The growth characteristics and production of color pigments by Monascus strains were investigated during liquid culture, and production of monacolin K in red mold rice was carried out by solid state fermentation. Four different Monascus ruber strains were cultured in potato dextrose yeast extract broth (PDYB) media at $25^{\circ}C$ for 15 days. The high producing strain for red pigment was not corresponded to the strain for yellow pigment. Production of red pigment was high in the strain causing the fast pH change in culture broth. Production of monacolin K in red mold rice by solid state fermentation was influenced by a combination of wet cell weight and spore density in inoculum by liquid culture. Most strains showed the high production of monacolin K in red mold rice, when submerged fermentation was carried out for 5 days as inoculum for solid state fermentation. These results suggest that submerged fermentation period of inoculum have an effect on the production of monacolin K in red mold rice by solid state fermentation, and monacolin K in red mold rice could be increased by controlling the condition of submerged fermentation for inoculum.

Mixture of Wild Panax Ginseng and Red-Mold Rice Extracts Activates Macrophages through Protection of Cell Regression and Cytokine Expression in Methotrexate-Treated RAW264.7 Cells

  • Shin, Heung-Mook
    • 대한한의학회지
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    • 제30권6호
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    • pp.69-79
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    • 2009
  • Objective: In this study, the immunomodulatory activity of a mixture of wild Panax ginseng and red-mold rice extracts (MPR) on RAW 264.7 macrophage cells in the presence and absence of methotrexate (MTX), an anti-cancer drug, was investigated. Methods and Results: In the cell viability, MPR showed a significant cell proliferation and inhibited cell regression by red-mold rice (RMR) alone or MTX alone. MPR induced moderate increase in nitric oxide (NO) production. NO production and inducible nitric oxide synthase (iNOS) mRNA expression by LPS decreased after MPR treatment. In addition, MPR slightly induced COX-2 mRNA expression, but it did not affect the expression of COX-2 mRNA by LPS treatment. In RT-PCR analyses, MPR induced IL-$1{\alpha}$, IL-$1{\beta}$, IL-6, and TNF-$\alpha$ mRNA expression, but had no effect on IL-10 and TGF-$\beta$, regardless of MTX treatment. Furthermore, MPR did not interfere with the cytotoxicity of MTX against MCF-7 human breast carcinoma cells. Conclusions: MPR is efficacious in protecting against MTX-induced cell regression as a result of macrophage activation, resulting in induction of cytokine expression, implying that MPR could be considered an adjuvant in MTX-chemotherapy.

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발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

  • 강성국;임종환;정순택;김선재
    • 한국미생물·생명공학회지
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    • 제24권6호
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    • pp.756-762
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    • 1996
  • In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultiva- ting the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30$\circ$C, initial pH 6.0, rice powder 3-5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1 vvm. Optimum temperature for producing yellow pigments was around 35$\circ$C which is higher than that of producing red pigments. The initial pH and rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. The optimum agitation speed was 100 - 300 rpm for pigment production.

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변질된 건조고추에서 분리한 곰팡이의 독소 생성 및 독성작용 (Mycotoxin Production and Animal Toxicity of Molds Isolated from Discolored Sun-Dried Red Pepper(Capsicum annuum L.))

  • 권선향;심선택;박은주;경규항
    • 한국식품위생안전성학회지
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    • 제23권3호
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    • pp.177-181
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    • 2008
  • 희나리 고추에서 분리한 곰팡이를 현미에 배양하여 실험동물(rat)에 투여하였을 때 Colletotrichum, Diaporthe, Diaporthopsis, Botryosphereia, Aspergillus, Fusarium의 경우 독성을 나타내지 않았으나 Alternaria alternata로 동정된 곰팡이는 독성을 나타내었다. A. alternata를 배양한 현미를 투여한 실험동물은 사료 섭취량이 상대적으로 낮았으며, 체중 감소를 보였고 2주안에 치사하였으며, 위, 소장, 간의 확대가 관찰되었다. Alternaria속 곰팡이는 17종이 분리되었으며 이들 중 8종은 고추즙과 현미에 배양하였을 때 모두 상당량의 tenuazonic acid를 생산하였고, 현미 배지에서만 alternariol과 alternariol monomethyl ether 독소가 추가로 소량 검출되었다. 건조 과정에서 곰팡이가 번식된 고추는 유해한 대사물의 존재 가능성이 있으므로 식품 안전을 위해 곰팡이 번식을 최대한 억제할 필요가 있다.

쌀 저장중의 곰팡이와 저곡해충 -제 1 보. Tribolium castaneum Herbst와 Aspergillus species의 저장미에서의 생육- (Activities of Molds and Insects during Rice Storage -Part I. Activities of Tribolium castaneum Herbst and Aspergillus species-)

  • 김영배;유문일
    • Applied Biological Chemistry
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    • 제25권4호
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    • pp.252-256
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    • 1982
  • Tribolium castaneum은 건전미입(健全米粒)에서 완전한 생활환(生活環)을 완성(完成)하여 쌀에 가해가능성(加害可能性)을 보였으며 그 활동(活動)은 1.3%의 수분함량의 경우는 증가를 초래하였으나 곰팡이의 증식은 오히려 저해하는 경향을 보였다. T. castaneum의 생육은 Asp. repens에 의해 안정화(安定化)되었으나 Asp. niger에 의해서는 지체되었다.

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홍국쌀을 첨가한 두부 셰이크의 제조기술 (Manufacture technology of tofu shake added with red rice)

  • 조은정;이상수;권동진
    • 한국식품저장유통학회지
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    • 제24권7호
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    • pp.942-948
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    • 2017
  • 본 연구에서는 홍국쌀에서 분리한 M. purpureus KCCM 60570을 찐 쌀에 1.0% (w/w) 접종 후 $25^{\circ}C$에서 배양하면서 홍국쌀 제조 조건 확립 및 품질 특성을 확인하였다. 또한 기존의 두부 셰이크에 홍국쌀을 첨가한 두부 셰이크의 기호도 조사를 통해 적정한 혼합비율을 결정하고, 품질 특성을 확인하였다. M. purpureus KCCM 60570으로 제조한 홍국쌀의 monacolin K와 곰팡이수를 확인한 결과 배양 8일차까지 급격히 증가하다가 이후 완만히 증가하여 홍국쌀의 적정제조온도 및 시간은 $25^{\circ}C$에서 8일로 나타났다. 기존의 두부 셰이크에 M. purpureus KCCM 6057으로 $25^{\circ}C$에서 8일간 제조한 홍국쌀을 서로 다른 농도로 첨가한 두부 셰이크의 관능검사 결과 맛, 향, 조직감 및 전체적인 기호도에서 0.5%(w/w) 홍국쌀을 첨가한 두부 셰이크의 기호도가 가장 높은 것으로 나타나 두부 셰이크의 적정 홍국쌀 혼합 비율은 0.5% (w/w)인 것으로 나타났다. 홍국쌀을 0.5% (w/w) 첨가한 두부 셰이크와 기존 시판 두부 셰이크의 품질 특성을 비교한 결과 홍국쌀 첨가 두부 셰이크의 환원당과 총당함량, 붉은 색소인 monacolin K와 색도 a 값이 월등히 높은 것으로 나타났다. 또한 기존 두부 셰이크와 0.5% (w/w) 홍국쌀을 첨가한 두부 셰이크와의 관능검사를 비교한 결과 색과 조직감이 좋은 것으로 나타나 두부 셰이크 제조에 홍국쌀을 첨가할 경우 두부 셰이크의 품질을 개선할 수 있는 가능성을 확인할 수 있었다.

TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구 (The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals)

  • 한양일;김광호;오영복
    • 한국환경보건학회지
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    • 제5권1호
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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홍곡곰팡이를 이용한 식용적색 색소의 제조 및 이의 성상에 관한 연구 (Studies on Production and Characteristics of Edible Red Color Pigment Produced by Mold(Monascus sp.))

  • 김창식;이숙희;김일
    • 한국식품과학회지
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    • 제9권4호
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    • pp.277-283
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    • 1977
  • 1. 증미(고체) 배양에서는 배양주(Type cultures)보다 분리주가 월등하게 많은 량의 색소를 생성하였다. 2. Lin씨(氏)의 Submerged culture에서는 IAM 8004(Monascus purbigerus)의 경우 carbon source로서 Rice powder 대신에 Wheat bran 1%, Starch 2%와 Corn meal 3%의 것이 색소의 생성량이 많았고, 분리주는 Carbon source를 Rice bran 1%, Starch 2%로 한 것이 가장 좋았다. 3. 사천씨(四川氏) 배지에서는 3%의 탈지대두분을 첨가했을 때 대체적으로 각 균주모두가 더 많은 색소의 생성을 보였다. 4. 추출한 색소액은 pH, 열처리등에 모두 안정하고 각종용매에 대한 추출성도 좋아서 많은 종류의 식품착색에이용될 수 있을 것으로 생각되나 내광성이 약한것이큰결점으로 보인다. 5. 본색소의 안전성은 Mouse의 경우 $LD_{50}$이 체중20g당 0.2359g으로서 안전한 편이며 더구나 시료를 25%의 에틸알코올 추출액으로 투여했기 때문에 에틸알코올의 독성을 감안한다면 색소자체는 극히 안전한 것으로 사려된다. 본연구는 산학협동재단의 학술연구비로서 이루워 진 것이다.

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쌀가루 전용 품종으로 제조한 약과의 품질 특성 (Quality Characteristics of Yakwa Produced with Rice Flour Developed as Rice Powder)

  • 이연리;유예진;박주영;신아현;김지수;김지연
    • 한국식품영양학회지
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    • 제32권5호
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    • pp.571-579
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    • 2019
  • For the purpose of promoting the consumption of rice powder, 5 cultivars were applied differently to establish the optimal manufacturing conditions and measured Hunter color values, hardness and expansion rate of Yakgwa for the optimal conditions. Looking into the optimal manufacturing conditions of Yakgwa, the commercial flour-facilitating Yakgwa is shown to have a great shape when the mold is made with a thick paste for C-2 manufacturing method, Hangaru 100 mesh for H100-2 manufacturing method, Hangaru 200 mesh for H200-2 manufacturing method, Shingil 100 mesh for S100-2 manufacturing method, and Shingil 200 mesh for S200-1 manufacturing method. It also showed a trend of chewy taste after frying without cracks. Hunter color values (L) and red chromaticity and value b of yellow chromaticity indicated a lower value for the commercial flour and Hangaru 200 mesh in its entirety. The hardness of Yakgwa is within the range of 1,981.41~3,756.45 g and the degree of hardness is shown differently for each rice powder exclusive cultivars. Rice flour developed as rice powder 5 cultivars, and as a result of measuring chromaticity, hardness and expansion rate of Yakgwa for the quality characteristics, the item of hangaru 200 mesh had outstanding quality characteristics for Yakgwa.

액체배양에서의 홍국균의 생장, 색소 및 monacolin K 생산 특성 (Characteristics of Growth, Pigment and Monacolin K Production by Monascus strains in Liquid Culture)

  • 서진원;김창섭;서은정;전체옥;최형균;박윤제
    • KSBB Journal
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    • 제27권5호
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    • pp.301-307
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    • 2012
  • The hyphal growth, production of color pigments and monacolin K by Monascus strains were investigated in liquid medium. Thirty five different strains were collected and cultured in potato dextrose yeast extract broth (PDYB), potato dextrose broth (PDB) and malt extract broth (MEB) medea at $25^{\circ}C$ for 7 days. The growth rates of most of strains were highest in PDYB medium. Growth rate as well as pigment production were influenced by suspension conditions of mycelia during liquid cultivation. Most of strains producing monacolin K corresponded to strains producing red pigment highly and showing more pH changes of liquid media. Monacolin K produced from strains was detected in culture broth as well as mycelia. Any citrinin was not detected in monacolin K producing strains. These results imply that the selection of the strains producing red pigment highly and showing more pH changes in liquid cultivation could be applied for primary screening of Monascus strains for preparation of red mold rice.