Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Mycotoxin Production and Animal Toxicity of Molds Isolated from Discolored Sun-Dried Red Pepper(Capsicum annuum L.)

변질된 건조고추에서 분리한 곰팡이의 독소 생성 및 독성작용

  • Published : 2008.09.30
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Abstract

Mycotoxins produced by molds isolated from discolored sun-dried red pepper fruits were determined and the toxicity to animals was also tested by feeding mold-grown unpolished rice to rats. Among the mold species tested, only Alternaria alternata was toxic to experimental animals, while other mold species belonging to the genera of Colletotrichum, Diaporthe, Diaporthopsis, Botryosphereia, Aspergillus and Fusarium were not. Rats fed Alternaria-grown rice lost weight and died within two weeks of feeding period. Succumbed rats during the process of feeding study showed extreme cases of enlargements of stomach, small intestine and liver. Among the 17 Alternaria isolates, 8 species produced considerable amount of tenuazonic acid along with small amounts of other toxins including alternariol and monomethyl ether derivative of alternariol in both red pepper homogenate and unpolished rice. It is therefore advised that red pepper fruits infested by molds during the sun-drying process be discarded to avoid unnoticeable health hazards.

희나리 고추에서 분리한 곰팡이를 현미에 배양하여 실험동물(rat)에 투여하였을 때 Colletotrichum, Diaporthe, Diaporthopsis, Botryosphereia, Aspergillus, Fusarium의 경우 독성을 나타내지 않았으나 Alternaria alternata로 동정된 곰팡이는 독성을 나타내었다. A. alternata를 배양한 현미를 투여한 실험동물은 사료 섭취량이 상대적으로 낮았으며, 체중 감소를 보였고 2주안에 치사하였으며, 위, 소장, 간의 확대가 관찰되었다. Alternaria속 곰팡이는 17종이 분리되었으며 이들 중 8종은 고추즙과 현미에 배양하였을 때 모두 상당량의 tenuazonic acid를 생산하였고, 현미 배지에서만 alternariol과 alternariol monomethyl ether 독소가 추가로 소량 검출되었다. 건조 과정에서 곰팡이가 번식된 고추는 유해한 대사물의 존재 가능성이 있으므로 식품 안전을 위해 곰팡이 번식을 최대한 억제할 필요가 있다.

Keywords

References

  1. Burroughs, R., Seitz, L.M., Sauer, D.B. and Mohr, H.E.: Effect of substrate on metabolite production of Alternaria alternata. Appl. Environ. Microbiol., 31, 685-690 (1976)
  2. Cho, M.-C., Jung, J.-W. and Cho, Y.-S.: Current status of national hot pepper industry in Korea. J. Kor. Capsicum Res. Coop., 10, 9-47
  3. Chung, G..J.: Food Composition Table (6th Ed.), National Rural Living Sci. Inst., Rural Development Adm., Korea, Sangrogsa, pp. 92 (0000)
  4. Chung, H.S.: Cereal scab causing mycotoxicoses in Korea and present status of mycotoxin researches. Kor. J. Mycol., 3, 31-36 (1975)
  5. Chung, I-M., Ju, H.-J., Shim, S.-C., Paik, S.-B. and Yu, S.-H.: Survey and control of the occurrence of mycotoxins from postharvest vegetables in Korea (II) Detection of major mycotoxins from diseased spice vegetables (onions, garlics and peppers). Anal. Sci. Technol., 11, 206-211 (1998)
  6. Griffin, G.. F. and Chu, F.S.: Toxicity of the Alternaria metabolites alternariol, alternariol methyl ether, altenuene, and tenuazonic acid in the chicken embryo assay. Appl. Environ. Microbiol., 46, 1420-1422 (1983)
  7. Gupta, J., Pathak, B., Sethi, N. and Vora, V.C.: Histopathology of mycotoxicosis produced in Swiss albino mice by metabolites of some fungal isolates. Appl. Environ. Microbiol., 41, 752-757 (1981)
  8. Hamilton, P.B., Lucas, G.B. and Welty, R.E.: Mouse toxicity of fungi of tobacco. Appl. Envron. Microbiol., 18, 570-574 (1969)
  9. Han, E.M., Park, H.R., Hu, S.J., Kwon, K.-S., Lee, H., Ha, M.-S., Kim, K.-M., Ko, E.-J., Ha, S.-D., Chun, H.S., Chung, D.-H. and Bae, D.-H.: Monitoring of aflatoxin B1 in livestock feeds using ELISA and HPLC. J. Microbiol. Biotechnol., 16, 643- 646 (2006)
  10. Harwig, J., Scott, P.M., Stoltz, D.R. and Blanchfield, B.J.: Toxins of molds from decaying tomato fruits. Appl. Environ. Microbiol., 38, 267-274 (1979)
  11. Kim, K.-H. and Chun, J.-K.: The effects of the hot air drying of red pepper on the quality. Kor. J. Food Sci. Technol., 7, 69-73 (1975)
  12. Kim, W.G.. and Yu, S.H.: A black mold of pepper fruits caused by Alternaria alternata. Kor. J. Plant Pathol., 1, 67-71 (1985)
  13. King, Jr.A.A. and Schade, J.E.: Alternaria toxins and their importance in food. J. Food Prot., 47, 886-901 (1984) https://doi.org/10.4315/0362-028X-47.11.886
  14. Ko, H.J., Choi, J.H., Kim, D.S., Yoo, Y.J. and Kyung, K.H.: Mycology of red pepper (Capsicum annuum L.) fruits discolored due to mold growth during sun-drying. Food Sci. Biotechno. l, 13, 627-631 (2004)
  15. Kwon, D.-J., Jo, J.-H., Kim, H.-K. and Park, M.-H.: Establishment of long-term storage condition of fresh re pepper paste. Kor. J. Food Sci. Technol., 22, 415-420
  16. Lee, S.W.: Information of red pepper. Taekyungnongsan, pp. 99 (1999)
  17. Lee, C.-H., Choi, E.-H., Kim, H.-S. and Lee, S.-R.: Storage stability and irradiation effect of red pepper powder. Kor. J. Food Sci. Technol., 9, 199-204 (1977)
  18. Lee, H.B. and Yu, S.H.: Incidence of Alternaria species in red pepper and sesame from Korea and their ability to produce mycotoxins. Kor. J. Plant Pathol., 11, 1-8 (1995) https://doi.org/10.1016/S0736-4539(06)80003-6
  19. Lee, H.B. and Yu, S.H.: Distribution of mycotoxin-producing isolates in the genus Alternaria. Kor. J. Plant Pathol., 11, 151-157 (1995)
  20. Lee, U.S., Jang, H.S., Tanaka, T., Toyasaki, N., Sugiura, Y., Oh, Y.J., Cho, C.M. and Ueno, Y.: Mycological survey of Korean cereals and production of mycotoxins by Fusarium isolates. Appl. Environ. Microbiol., 52, 1258-1260 (1986)
  21. Logrieco, A., Bottalico, A., Visconti, A. and Vurro, M.: Natural occurrence of Alternaria-mycotoxins in some plant products. Microbiol. Alim. Nutr., 6, 13-17 (1988)
  22. Magan, N., Cayley, G..R. and J. Lacey.:Effect of water activity and temperature on mycotoxin production by Alternaria alternata in culture and on wheat grain. Appl. Environ. Microbiol., 47, 1113-1117 (1984)
  23. Meronuck, R.A., Steele, J.A., Mirocha, C.J. and Christensen, C.M.: Tenuazonic acid, a toxin produced by Alternaria alternata. Appl. Environ. Microbiol., 23, 613-617 (1972)
  24. Mislivec, P.B., Fieter, C.T. and Bruce, V.R.: Mycotoxin-producing potential of mold flora of dried beans. Appl. Environ. Microbiol., 27, 522-526 (1975)
  25. Pero, R.W., Posner, H., Blois, M., Harvan, D. and Spalding, J.W.: Toxicity of metabolites produced by the Alternaria. Environ. Health Persp., June, 87-94 (1973)
  26. Schroeder, H.W. and Cole, R.J.: Natural occurrence of alternariol in discolored pecans. J. Agr. Food Chem., 25, 204-206 (1977) https://doi.org/10.1021/jf60209a032
  27. Shigeura, H.T. and Gordon, C.N.: The biological activity of tenuazonic acid. Biochemistry, 2, 1132-1137 (1963) https://doi.org/10.1021/bi00905a039
  28. Stinson, E.E., Osman, S.F., Heisler, E.G.., Siciliano, J. and Bills, D.D.: Mycotoxin production in whole tomatoes, apples, oranges, and lemons. J. Agric. Food Chem., 29, 790-792 (1981) https://doi.org/10.1021/jf00106a025
  29. Watson, D.H.: An assessment of food contamination by toxic products of Alternaria J. Food Prot., 47, 485-488 (1983)
  30. Yu, S.H.: Significance of sesame seed-borne fungi, with special reference to Corynespora cassiicola. Kor. J. Plant. Pathol., 20, 183-190