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Effect of Seed Mixture on the Forage Yield and Botanical Composition in the Hanwoo Grazing Pasture (한우 방목초지에서 목초 혼파조합이 식생구성과 수량에 미치는 영향)

  • Kim Meng-Jung;Cho Young-Moo;Choi Sun-Ho;Kim Young-Gun;Yoon Sei-Hyung;Kim Jong-Geun;Yook Wan-Bang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.2
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    • pp.113-120
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    • 2006
  • This study was conducted to select the optimum seed mixture for beef cattle (Hanwoo) grazing in middle mountain area at 450m from sea level during 2 years from October 1, 1999. Seed mixtures were composed of four different treatment according to main species such as orchardgrass (T1), perennial ryegrass (T2), timothy (T3) and Italian ryegrass (T4). Grass composition rate of different seed mixtures was decreased mostly at first year of grazing, like 44.9% of orchardgrass in T1 plot, 27.6% of perennial ryegrass in T2 plot, 40.9% of timothy in T3 plot and 40.7% of Italian ryegrass in T4 plot, respectively. Main species in seed mixture were decreased in grazing pasture, but kentucky bluegrass and tall fescue were increased in all plots. Italian ryegrass in T4 plot was well grown in early grown stage and inhibited the growth of other grass species, and 44.2% of Italian ryegrass was maintained in second year in the grazing plot. Average dry matter yields of first you showed 6,673 kg/ha and second year was 10,177 kg/ha. Average TDN yields of different seed mixtures were 6,399 kg/ha in T1, 7,769 kg/ha in T2, 4,383 kg/ha in T3 and 7,146 kg/ha in T4 plot. Average herbage dry matter intakes by different seed mixtures during 2 years were 5,937 kg/ha in T1, 6,375 kg/ha in T2, 4,925 kg/ha in T3 and 6,456 kg/ha in T4. This result indicated that perennial ryegrass would recommended in main species of Hanwoo grazing pasture.

Sudies on the of $K_2O$Fertilization for the Pasturu Improvement (초지개량에 미치는 가리시비의 효과에 관한 연구)

  • 권순기;김용국;김문규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.3 no.1
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    • pp.17-25
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    • 1982
  • To clarify the direct effect of potassium and interactions with nitrogen and phosphorus on the yield of pasture plants, fertilizer experiment with varied levels of potassium and nitrogen treatment to grass single seeding, legume single seeding and grass-legume mixed seeding, was conducted for three years from 1977 to 1980. The summary of obtained experimental result may be described as below: 1. Potassium application, at any level of amount, did not show its effect to the establishment of plant population, however potash application responded to help winter hardiness at grass single seeding plot. 2. Potassium effect on the fresh yield at grass single seeding was distinct however, not clear effect was observed at legume single seeding and grass-legume mixed seeding plots. 3. For drymatter yield, the highest yield at legume single seeding was recorded 1.152 Kilograms per 10a are at treatment #4 and the highest yields of grass-legume mixed seeding and grass were recorded 1,093 kilograms (Treatment #3) and 834 kilograms (Treatment #5) respectively. 4. In observation of plant succession, they were found that orchardgrass and tall fescue have increased at grass single seeding plot and Alfalfa was dominant over 90 per cent of plant population at legume single seeding and grass-legume mixed seeding plots. It seems that potash effect was distinct in increasing orchardgrass population. 5. The results of this experiment shown that the maximum yield from grass single seeding was obtained at the plat where applied 20-20-30 kg/10a, $N-P_2O_5-K_2O$ and the maximum yield from legume single seeding was obtained at the plot applied with 8-20-20 kg/10a, $N-P_2O_5-K_2O$. 6. Fertilizer cost for producing one kilogram of dry matter in this experiment was lowest at treatment #1 of grass-legume mixed seeding and highest at treatment #5 of grass single seeding. The largest gross income was obtained from the plots where phosphate application was omitted. 7. From the results of this experiment, it may be recommended that economically optimum fertilizer application for grass single seeding will be 20-20-20- kg/10a, $N-P_2O_5-K_2O$ and for legume single seeding and grass-legume mixed seeding will be 8-20-20 kg/10a, $N-P_2O_5-K_2O$.

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Effects of the Additives on the Quality of Alfalfa Silage (첨가제 처리가 알팔파 사일리지의 품질에 미치는 영향)

  • Kim, J.G.;Chung, E.S.;Kang, W.S.;Ham, J.S.;Kim, J.D.;Seo, S.;Lee, J.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.115-120
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    • 1999
  • This experiment was carried out to investigate the effects of additives on the quality of alfalfa(Medicago sativa L.) silage at the forage experimental field, National Livestock Research Institute, RDA, Suwon in 1996. The experiment was arranged in a randomized block design with three replications. The treatments consisted of different additives(control, formic acid, molasses, inoculant A and inoculant B). Crude protein(CP) and Nitrogen free extract(NFE) content of alfalfa silage with additives were higher compared with those obtained control, but Crude ash(CA) and Crude fiber(CF) content of control(11.5 and 39.6%) were higher than those of additive treated plots. ADF and NDF contents of control silage were the highest as 36.2 and 48.6%, respectively, and increased by 4% compared with those obtained before ensiling. The acidity(pH) of control was the highest(5.45), but the lowest in inoculant A(4.32). Inoculant teratment significantly reduced acidity of silage compared with control. DM content of control silage was the lowest but DM loss of control silage was higher than that of additive treated silage. Ammonia-N content was the highest at formic acid treatment and level of Lactobacillus was decreased at control and formic acid treated silage. Total organic acid and lactic acid content of inoculant A and inoculant B were higher than those of other treated plots. The results of this study indicate that the treatment of LAB(Lactic acid bacteria) could be recommended as producing high quality of alfalfa silage.

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Coupled T-H-M Processes Calculations in KENTEX Facility Used for Validation Test of a HLW Disposal System (고준위 방사성 폐기물 처분 시스템 실증 실험용 KENTEX 장치에서의 열-수리-역학 연동현상 해석)

  • Park Jeong-Hwa;Lee Jae-Owan;Kwon Sang-Ki;Cho Won-Jin
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.4 no.2
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    • pp.117-131
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    • 2006
  • A coupled T-H-M(Thermo-Hydro-Mechanical) analysis was carried out for KENTEX (KAERI Engineering-scale T-H-M Experiment for Engineered Barrier System), which is a facility for validating the coupled T-H-M behavior in the engineered barrier system of the Korean reference HLW(high-level waste) disposal system. The changes of temperature, water saturation, and stress were estimated based on the coupled T-H-M analysis, and the influence of the types of mechanical constitutive material laws was investigated by using elastic model, poroelastic model, and poroelastic-plastic model. The analysis was done using ABAQUS, which is a commercial finite element code for general purposes. From the analysis, it was observed that the temperature in the bentonite increased sharply for a couple of days after heating the heater and then slowly increased to a constant value. The temperatures at all locations were nearly at a steady state after about 37.5 days. In the steady state, the temperature was maintained at $90^{\circ}C$ at the interface between the heater and the bentonite and at about $70^{\circ}C$ at the interface between the bentonite and the confining cylinder. The variation of the water saturation with time in bentonite was almost same independent of the material laws used in the coupled T-H-M processes. By comparing the saturation change of T-H-M and that of H-M(Hydro-Mechanical) processes using elastic and poroelastic material mod31 respectively, it was found that the degree of saturation near the heater from T-H-M calculation was higher than that from the coupled H-M calculation mainly because of the thermal flux, which seemed to speed up the saturation. The stresses in three cases with different material laws were increased with time. By comparing the stress change in H-M calculation using poroelasetic and poroelasetic-plastic model, it was possible to conclude that the influence of saturation on the stress change is higher than the influence of temperature. It is, therefore, recommended to use a material law, which can model the elastic-plastic behavior of buffer, since the coupled T-H-M processes in buffer is affected by the variation of void ratio, thermal expansion, as well as swelling pressure.

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Analysis of newborn hearing screening using automated auditory brainstem response (자동화 청성뇌간반응을 이용한 신생아 청력선별검사 결과 분석)

  • Park, Sung Won;Yun, Byung Ho;Kim, Kyung Ah;Ko, Sun Young;Lee, Yeon Kyung;Shin, Son Moon;Hong, Sung Hwa
    • Clinical and Experimental Pediatrics
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    • v.49 no.10
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    • pp.1056-1060
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    • 2006
  • Purpose : As hearing ability affects language and cognitive development, early detection and intervention of congenital hearing defects is very important. We analyzed the result of newborn hearing screening using automated auditory brainstem response and estimated the incidence of congenital hearing defects in newborn infants in Korea. Methods : Hearing screening tests were done on 7,218 newborn infants who were delivered at Cheil General Hospital from July 1, 2004 to June 30, 2005. The first screening test was done on the second day of life with automated auditory brainstem response(AABR) using $ALGO{\bigcirc}^{(3)}$ Newborn hearing screener($Natus^{(R)}$ Medical Incorporated, San Carlos, USA) with 35 dB sound level. The newborn infants who did not pass the initial screening test took the second screening AABR test before discharge from the nursery. Infants who did not pass these screenings at the nursery were followed up at the Department of Otorhinolaryngology, Samsung Seoul Hospital. Results : Total 7,218 infants(83.3 percent of total 8,664 live births of the Cheil General Hospital) were screened in the nursery, and 55 of them failed to pass the newborn screening. Among 55 infants who were referred, six were lost during follow-up, and 14 were confirmed as hearing impaired. Six of them(42.8 percent) do not have any risk factors for hearing impairment. We can estimate that the incidence of hearing defects is about 1.9-2.8 per 1,000 live births. Conclusion : Automated auditory brainstem response is an effective tool to screen the hearing of newborn infants. Congenital hearing loss is more frequent than metabolic diseases on which screening tests are available in the newborn period. About 40 percent of infants who have hearing defects do not have any risk factors for hearing impairment. Therefore, universal newborn hearing screening must be recommended to all neonates.

Characterization of Bruton's Tyrosine Kinase Genetic Mutations in One Korean X-linked Agammaglobulinemia Family (반성 열성 범저감마글로불린혈증 1가계 3환자의 Bruton's Tyrosine Kinase 유전자 변이 및 임상 양상)

  • Jo, Eun-Kyeong;Song, Chang-Hwa;Park, Jeong-Kyu;Baek, Young-Jong;Rhu, Hye-Young;Lee, Jae-Ho;Hwang, Tai-Ju;Kook, Hoon
    • Clinical and Experimental Pediatrics
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    • v.45 no.2
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    • pp.183-191
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    • 2002
  • Purpose : X-linked agammaglobulinemia(XLA) is an immunodeficiency caused by abnormalities in Bruton's tyrosine kinase(Btk), and is characterized by a deficiency of peripheral blood B cells. We studied the cytoplasmic expression of Btk protein and analyzed the Btk gene in peripheral blood mononuclear cells from two siblings and one cousin with XLA, as well as additional family members. Methods : Btk protein expression was analyzed by flow cytometry. Isolation of the coding sequence of the Btk gene was performed by amplification using the reverse transcription-polymerase chain reaction(RT-PCR) technique. Sequence alterations were screened by the single-stranded conformation polymorphism(SSCP) method and characterized by standard sequencing protocols. Results : Cytoplasmic expression of Btk protein in monocytes was not detected in three patients with XLA. In addition, Btk protein analysis clearly showed cellular mosaicism in monocytes from four obligate carriers, findings further supported by SSCP. A single base pair mutation(T to C) in Btk-exon three, which encodes the PH domain, was identified in four XLA patients. A diagnostic sequencing analysis was established to detect heterozygotic pattern in 4 carrier females. Furthermore, we found significant clinical heterogeneity in individuals with the same gene mutation. Conclusion : The implicating genetic alteration provided valuable clues to the pathogenesis of XLA in Korea and the flow cytometric analysis was suggested as a useful tool for rapid detection of XLA patients and carriers. The present study has identified a genetic mutation in the Btk coding region and demonstrated heterogeneity in clinical manifestations among patients with the same mutation. A flow cytometric analysis was found to be informative in establishing a deficiency of Btk protein in both patients and carriers and is recommended as a frontline procedure in the molecular diagnosis and work-up of XLA.

Effect of Amount of Oil Cake Applications on Mineral Nutrient Partitioning of Black Chokeberry (유박시용량에 따른 유기 블랙초크베리의 수체 내 무기성분 분배에 미치는 영향)

  • Choi, Hyun-Sug;Jung, Seok-Kyu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.28 no.1
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    • pp.5-14
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    • 2020
  • The study was initiated to reduce production cost and environmental pollution with the evaluation of nutrient requirement of 'Nero' black chokeberry (Aronia melanocarpa) and optimum amount of oil cake application. 100% of a recommended amount (RA) of oil cake was designated as a 100-RA, with 0-RA, 25-RA, 50-RA, and 75-RA for 0%, 25%, 50%, and 75% RA, respectively. The oil cake was scattered around the black chokeberry at every year for two years from 2018 to 2019, with investigation conducted for the second year. Soil mineral nutrient concentrations were not significantly different among the treatments. Dry weight (DW) of root and leaves was low for 0-RA-treated black chokeberry, with no significant difference observed for the all treatments for the DW of stems. 75-RA increased the fruit DW of 615 g and yield efficiency of 45.3%. Top:root ratio was the highest of 4.7 for 75-RA. Increased amount of oil cake application expanded the tree volume. Tree volume had a strong positive relationship with the root DW (r2=0.977). Mineral nutrient uptake in the root was the highest on the 25-RA-treated black chokeberry, except for Fe uptake. Mineral nutrient uptake in the leaves were similar to all the black chokeberries, except for T-N and Fe uptake. 75-RA increased mineral nutrient uptake in the fruit, except for Cu, in particular, 7.45 g in fruit N, which was the highest level compared to those of the other organs. T-N and P uptake were evenly distributed in the leaves, stems, and fruit, with high K uptake for leaves and fruit. 75-RA maximized to 17.2 g of the total nutrient uptake in a black chokeberry, with 4.9 g for the 0-RA. All mineral nutrient uptake were overall higher on the black chokeberry treated with 50-RA, 75-RA, and 100-RA compared to those of 0-RA and 25-RA.

Control of Phythophthora capsici and Residual Characteristics by the Pesticides Tank-Mixed in Tomato Hydroponic Culture System (농약의 양액 탱크내 혼합처리에 의한 토마토 역병 방제 효과 및 잔류 특성)

  • Ihm, Yang-Bin;Kyung, Kee-Sung;Kim, Cban-Sub;Park, Byung-Jun;Lee, Jung-Sup
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.264-270
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    • 2003
  • To control effectively and safely Phytophthora root rot caused by Phytophthora capsici on tomato in hydroponic culture, tank-mixing method was considered with two pesticides, metalaxyl copper oxychloride 50% WP and dimethomorph dithianon 38% WP. Forty days after transplanting of tomato seedlings, 4 mL of sporangia of P. capsici (about 25 sporangi/mL) per plot was inoculated around tomato plant roots, and at 5 days after inoculation, the pesticides tank-mixed at three dilution levels, 12,500, 25,000 and 50,000, were drenched 1, 2 or 3 times per plot on the culture cube every 15 days for metalaxyl copper oxychloride 50% WP and every 10 days for dimethomorph dithianon 38% WP. During the drenching period, the residue levels of metalaxyl and dimethomorph in hydroponic culture solution were similar to the initial levels but the level of dithianon was drastically decreased from one day after tank-mixing. In tomato drenched with metalaxyl copper oxychloride 50% WP, metalaxyl was detected $0.02\sim0.04$ mg/kg in all diluted plots. Dimethomorph was detected $0.012\sim0.021$, $0.001\sim0.006$ and $0.001\sim0.003$ mg/kg in 12,500, 25,000 and 50,000 times diluted plots, respectively, while dithianon was detected 0.005, 0.003 mg/kg in 12,500 and 50,000 times diluted plots, respectively. The detection levels of three pesticides were far below compared with the levels of Korean MRLs. Incidences of Phytophthora root rot were not found in all the plots, but phytotoxic responses were recognized in the 12,500 times diluted plots of both pesticides. Based on the above results, the drenching of the culture solution tank-mixed with these pesticides could be recommended as a very safe and effective method to control Phytophthora root rot in tomato in hydroponic culture.

The Effect of Consumer's Perceptual Characteristics for PB Products on Relational Continuance Intention: Mediated by Brand Trust and Brand Equity (PB상품에 대한 소비자의 지각특성이 관계지속의도에 미치는 영향: 브랜드신뢰 및 브랜드자산을 매개로 한 정책적 접근)

  • Lim, Chaekwan
    • Journal of Distribution Research
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    • v.17 no.5
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    • pp.85-111
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    • 2012
  • Introduction : The purpose of this study was to examine the relationship between perceptual characteristics of consumers and intent of relational continuance for PB(Private Brand) products in discount stores. This study was conducted as an empirical study based on survey. For the empirical study, factors of PB products as characteristics perceived by consumers such as perceived quality, store image, brand image and perceived value were deduced from preceding studies. The effect of such factors on intent of relational continuance mediated by brand trust and brand equity of PB products was structurally examined. Research Model : Based on theory analysis and hypotheses, constructed a Structural Equation Model(SEM). The research model is shown in Figure 1. Research Method : This paper is based on s qualitative study of selected literature and empirical data. The survey for empirical study was carried out on consumers in Gyeonggi and Busan between January 2012 and May 2012. 300 surveys were distributed and 253 (84.3%) of them were returned. After excluding omissions and insincere responses, 245 surveys (81.6%) were used for final analysis as effective samples. Result : First of all, the Reliability was carried out for instrument used. The lower limit of 0.7 for Cronbach's Alpha as suggested by Hair et al. (1998). And Construct validity was established by carrying out exploratory factor analysis by Varimax rotation for all. Four factor result for the consumer's perceptual characteristics of PB Products, two mediating factors and one dependent factor. All constructs included in research framework have acceptable validity and reliability. Table 1 shows the factor loading, eigen value, explained variance and Cronbach's alpha for each factor. In order to assure validity of constructs, I implemented Confirmatory Factor Analysis (CFA), using AMOS 20.0. In confirmatory factor analysis, researcher can take control over the specification of indicators for each factor by hypothesizing that a specific factor is loaded with the relevant indicators. Moreover, CFA is particularly useful in the validation of scale for the measurement of specific construct. CFA result summarized Table 2 shows that the fit measures of all constructs fulfill the recommended level and loadings are significant. To test causal relationship between constructs in the research model, used AMOS 20.0 that provides a graphic module as method for analysing Structural Equation Modeling. The result of hypothesis test is shown in Table 3. As a result of empirical study, perceived quality, brand image and perceived value as selected attributes for PB products showed significantly positive (+) effect on brand trust and brand equity. Furthermore, brand trust and brand equity showed significantly positive (+) effect on intent of relational continuance. However, store image of discount stores selling the PB products was analyzed to have positive (+) effect on brand trust and no significant effect on brand equity. Discussion : Based on the results of this study, the relationship between overall quality, store image, brand image and value perceived by consumers about PB products and intent of relational continuance was structurally verified as being mediated by brand trust and brand equity. Looking at the results, a strategic approach that maximizes brand trust and equity value for PB products by large discount stores is required on top of basic efforts to improve quality, brand image and value of PB products in order to maximize consumer's intent of relational continuance and to continuously attract repeated purchase of products.

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A COMPARATIVE STUDY ON RADIOPACITY OF CANAL FILLING AND RETROGRADE ROOT-END FILLING MATERIALS (근관 충전재 및 치근단 역충전재의 방사선 불투과성에 관한 비교 연구)

  • Kim, Yong-Sang;Kim, Seo-Kyong;Hwang, Yun-Chan;Hwang, In-Nam;Oh, Won-Mann
    • Restorative Dentistry and Endodontics
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    • v.33 no.2
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    • pp.107-114
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    • 2008
  • This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials. Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, $Dyract^{(R)}$ AP, Super $EBA^{(R)}$, $IRM^{(R)}$, AH $26^{(R)}$, $Sealapex^{TM}$, Tubli-$Seal^{TM}$, and dentin were evaluated in this study. In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 mm in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained. 1. Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp. 2. All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards. 3. Most of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract. It suggests that all experimental canal filling and retrograde root-end filling materials have a sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract.