• Title/Summary/Keyword: RecA

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Gated Recurrent Unit Architecture for Context-Aware Recommendations with improved Similarity Measures

  • Kala, K.U.;Nandhini, M.
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.2
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    • pp.538-561
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    • 2020
  • Recommender Systems (RecSys) have a major role in e-commerce for recommending products, which they may like for every user and thus improve their business aspects. Although many types of RecSyss are there in the research field, the state of the art RecSys has focused on finding the user similarity based on sequence (e.g. purchase history, movie-watching history) analyzing and prediction techniques like Recurrent Neural Network in Deep learning. That is RecSys has considered as a sequence prediction problem. However, evaluation of similarities among the customers is challenging while considering temporal aspects, context and multi-component ratings of the item-records in the customer sequences. For addressing this issue, we are proposing a Deep Learning based model which learns customer similarity directly from the sequence to sequence similarity as well as item to item similarity by considering all features of the item, contexts, and rating components using Dynamic Temporal Warping(DTW) distance measure for dynamic temporal matching and 2D-GRU (Two Dimensional-Gated Recurrent Unit) architecture. This will overcome the limitation of non-linearity in the time dimension while measuring the similarity, and the find patterns more accurately and speedily from temporal and spatial contexts. Experiment on the real world movie data set LDOS-CoMoDa demonstrates the efficacy and promising utility of the proposed personalized RecSys architecture.

Rain Attenuation Analysis for Designing UAV Data Link on Ku-Band (Ku대역 무인항공기 데이터 링크 설계를 위한 강우감쇠 분석)

  • Lee, Jaeyoon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.7
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    • pp.1248-1256
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    • 2015
  • It is necessary to apply an exact data and a precise prediction model for a rain attenuation to design the link margin for a data link using Ku-band with the serious effect by rain. In this paper, we investigate the regional rainfall-rate distribution of Korea proposed in TTAK.KO-06.0122/R1 and compare it with the distribution provided by Rec. ITU-R PN.837-1 and Crane. And, the rain rate climate regions similar with the rainfall-rate distribution of Korea in Rec. ITU-R PN.837-1 and Crane model are selected. Finally, using Rec. ITU-R P.618-8 and Crane rain attenuation prediction model, we derive and analyze the rain attenuation for Ku-band frequency according to the time percentage of an average year and the distance of wireless communication link between unmanned aerial vehicle (UAV) and ground data terminal (GDT).

A Causal Recommendation Model based on the Counterfactual Data Augmentation: Case of CausRec (반사실적 데이터 증강에 기반한 인과추천모델: CausRec사례)

  • Hee Seok Song
    • Journal of Information Technology Applications and Management
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    • v.30 no.4
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    • pp.29-38
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    • 2023
  • A single-learner model which integrates the user's positive and negative perceptions is proposed by augmenting counterfactual data to the interaction data between users and items, which are mainly used in collaborative filtering in this study. The proposed CausRec showed superior performance compared to the existing NCF model in terms of F1 value and AUC in experiments using three published datasets: MovieLens 100K, Amazon Gift Card, and Amazon Magazine. Compared to the existing NCF model, the F1 and AUC values of CausRec showed 1.2% and 2.6% performance improvement in MovieLens 100K data, and 2.2% and 10% improvement in Amazon Gift Card data, respectively. In particular, in experiments using Amazon Magazine data, F1 and AUC values were improved by 11.7% and 21.9%, respectively, showing a significant performance improvement effect. The performance of CausRec is improved because both positive and negative perceptions of the item were reflected in the recommendation at the same time. It is judged that the proposed method was able to improve the performance of the collaborative filtering because it can simultaneously alleviate the sparsity and imbalance problems of the interaction data.

Characterization of the din (damage-inducible) and tin (temperature-inducible) Genes Isolated from Escherichia coli (대장균에서 분리된 din (damage-inducible)과 tin (temperature-inducible) 유전자들의 특성)

  • 백경희
    • Korean Journal of Microbiology
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    • v.29 no.6
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    • pp.392-396
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    • 1991
  • Mu d1(Ap lac) bacteriophage can be used to search for genes which are members of a common regulatory network without having to know the functions of the genes in advance. Aim was for obtaining the loci in the SOS network as well as temperature inducible loci. For this purpose, recA441 allele was used. This allele encodes a thermosensitive recA gene product; thus, the recA441 allele can be activated upon temperature upshift without by external DNA damage. Approximately 10, 000 colonies were screened, and then searched for the colonies which expressed .betha.-galactosidase higher level at 42.deg.C than at 30.deg.C. The strains identified fell into two dlasses; (i) ones in which the increased expression was $recA^{+}$ $lexA^{+}$ -dependent, that is, din(damage-inducible) genes which were due to the activation of recA441 allele and (ii) ones in which the increased expression was $recA^{+}$ $lexA^{+}$ -independent and only temperature-inducible, tin genes. Rough mapping position was obtained for these genes.

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$\beta$-Subunit 94~96 Residues of Tethered Recombinant Equine Chorionic Gonadotropin are Important Sites for Luteinizing Hormone and Follicle Stimulating Hormone like Activities

  • Park, Jong-Ju;JarGal, Naidansuren;Yoon, Jong-Taek;Min, Kwan-Sik
    • Reproductive and Developmental Biology
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    • v.34 no.1
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    • pp.33-40
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    • 2010
  • Equine chorionic gonadotropin (eCG) is a heavily glycosylated glycoprotein composed of non-covalently linked $\alpha$- and $\beta$-subunits. To study the function and signal transduction of tethered recombinant-eCG (rec-eCG), a single chain eCG molecule was constructed, and the rec-eCG protein was prepared. In this study, we constructed 5 mutants (${\Delta}1$, ${\Delta}2$, ${\Delta}3$, ${\Delta}4$, and ${\Delta}5$) of rec-eCG using data about known glycoprotein hormones to analyze the role of specific follicle stimulating homone (FSH)-like activity. Three amino acids of certain specific sites were replaced with alanine. The expression vectors were transfected into CHO cells and subjected to G418 selection for 2~3 weeks. The media were collected and the quantity of secreted tethered rec-eCGs was quantified by ELISA. The LH- and FSH-like activities were assayed in terms of cAMP production by rat LH/CG and rat FSH receptors. Then, the metabolic clearance rate analyzed by the injection of rec-eCG (5 IU) into the tail vein was analyzed. The mutant eCGs (${\Delta}l$, ${\Delta}4$, and ${\Delta}5$) were transcripted, but not translated into proteins. Rec-eCG A2 was secreted in much lower amounts than the wild type. Only the rec-eCG ${\Delta}3$ ($\beta$-subunit: $Gln^{94}-Ile^{95}-Lys^{96}{\rightarrow}Ala^{94}-Ala^{95}-Ala^{96}$) was efficiently secreted. Although activity is low, its LH-like activity was similar to that of tethered $eCG{\beta\alpha}$. However, the FSH-like activity of rec-$eCG{\beta\alpha\Delta}3$ was completely flat. The result of the analysis of the metabolic clearance rate shoed the persistence of the mutant in the blood until 4 hours after the injection. After then, it almost disappeared at 8 hours. Taken together, these data suggest that 94~96 amino acid sequences in eCG $\beta$-subunit appear to be of utmost importance for signal transduction of the FSH receptor.

Development of Light Distributed Solar Modules for Growing Sprout Ginseng and Overseas Empirical Research on Natural Lighting Cultivation System (새싹인삼 재배를 위한 투광 분산형 태양광 모듈 개발 및 자연채광 재배시스템 해외 실증연구)

  • Seong-Hwan Kang;Jae-ho Choi;In-Sung Jung;Gyeong-Yeol Lim;Jae-Woo Park;Nguyen Huu Hoang;Chong-Yeal Kim
    • Current Photovoltaic Research
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    • v.12 no.1
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    • pp.24-30
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    • 2024
  • To create an environment for growing ginseng sprouts, we developed a solar module that partially transmits and disperses light. A G to G type light transmission and dispersion solar module was developed using glass with a mist pattern applied, and the light dispersion effect of the developed module was confirmed through illuminance measurement. The output of one module is approximately 260 W, and the configuration consists of 48 cells in series in 4 strings. The cultivation system where the developed module will be installed was developed in the form of a container, and three units of 2.6 kW (260 W x 10 EA). The inside of the cultivation system consists of a shading screen, air conditioner, ventilator, plastic pot, etc. to create an environment for cultivating sprout ginseng. As a result of actually planting sprout ginseng, it was confirmed through verification that the plants were grown without any problems.

A Study of Optimum Capacity of Battery Energy Storage System Linked PV (태양광 연계형 배터리 에너지 저장장치의 최적 용량 산정)

  • Baek, Min-Kyu;Park, Jong-Bae;Son, Sung-Yong;Shin, Ha-Sang;Park, Yong-Gi
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.1
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    • pp.38-45
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    • 2018
  • In September 2016, the government decided to apply a REC 5.0 weighting to solar-battery ESS to increase the supply of renewable energy. In this paper, we calculated the optimal capacity of battery ESS which maximizes the revenue when solar is linked with battery ESS. In the case study, the optimal capacity was calculated by applying the conservative REC price, and we studied sensitivity analysis about battery price and real-time REC price.

Continuous Passaging of a Recombinant C-Strain Virus in PK-15 Cells Selects Culture-Adapted Variants that Showed Enhanced Replication but Failed to Induce Fever in Rabbits

  • Tong, Chao;Chen, Ning;Liao, Xun;Yuan, Xuemei;Sun, Mengjiao;Li, Xiaoliang;Fang, Weihuan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.9
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    • pp.1701-1710
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    • 2017
  • Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that $RecCpp40_{+10}$ failed to induce fever in rabbits, whereas $RecCpp80_{+10}$ caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.

Internalization of Rat FSH and LH/CG Receptors by rec-eCG in CHO-K1 Cells

  • Park, Jong-Ju;Seong, Hun-Ki;Kim, Jeong-Soo;Munkhzaya, Byambaragchaa;Kang, Myung-Hwa;Min, Kwan-Sik
    • Development and Reproduction
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    • v.21 no.2
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    • pp.111-120
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    • 2017
  • Equine chorionic gonadotropin (eCG) is a unique molecule that elicits the response characteristics of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in other species. Previous studies from this laboratory had demonstrated that recombinant eCG (rec-eCG) from Chinese hamster ovary (CHO-K1) cells exhibited both FSH- and LH-like activity in rat granulosa and Leydig cells. In this study, we analyzed receptor internalization through rec-eCGs, wild type eCG ($eCG{\beta}/{\alpha}$) and mutant eCG ($eCG{\beta}/{\alpha}{\Delta}56$) with an N-linked oligosaccharide at $Asn^{56}$ of the ${\alpha}-subunit$. Both the rec-eCGs were obtained from CHO-K1 cells. The agonist activation of receptors was analyzed by measuring stimulation time and concentrations of rec-eCGs. Internalization values in the stably selected rat follicle-stimulating hormone receptor (rFSHR) and rat luteinizing/chorionic gonadotropin receptor (rLH/CGR) were highest at 50 min after stimulation with 10 ng of $rec-eCG{\beta}/{\alpha}$. The dose-dependent response was highest when 10 ng of $rec-eCG{\beta}/{\alpha}$ was used. The deglycosylated $eCG{\beta}/{\alpha}{\Delta}56$ mutant did not enhance the agonist-stimulated internalization. We concluded that the state of activation of rFSHR and rLH/CGR could be modulated through agonist-stimulated internalization. Our results suggested that the eLH/CGRs are mostly internalized within 60 min by agonist-stimulation by rec-eCG. We also suggested that the lack of responsiveness of the deglycosylated $eCG{\beta}/{\alpha}{\Delta}56$ was likely because the site of glycosylation played a pivotal role in agonist-stimulated internalization in cells expressing rFSHR and rLH/CGR.

Development of Displacement Measuring Sensor for Roll Eccentricity Contro1 at Hot Strip Mill (롤 편심제어를 위한 편심 측정장치 개발)

  • 전종학
    • 제어로봇시스템학회:학술대회논문집
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    • 2000.10a
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    • pp.167-167
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    • 2000
  • This study aims at the development of REC sensor causing the gauge error in the hot strip rolling process, and the improvement of the hydraulic AGC (Automatic Gauge Control) system. The gauge error outbreaks from the various reasons, however, mainly the roll eccentricity is considered to cause a such kind of error. In the study, the REC (Roll Eccentricity Control System) sensor is designed using sensor An On - line test of the system shows the comprehensive effects of controlling the gauge error of the hot strip. On - Line test shows the possibility of enhancing the accuracy for gauge.

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