• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.16
• /
• pp.7031-7038
• /
• 2015

Background: Arginine may play important roles in tumor progression by providing ornithine for polyamine biosynthesis, required for cell growth. The aim of this work was to determine the expression of arginine metabolic pathway enzymes in head and neck squamous cell carcinoma (HNSCC) in northeast India. Materials and Methods: The expressions of arginase isoforms (ARG1 and ARG2), ornithine aminotransferase (OAT) and ornithine decarboxylase (ODC) were examined in fifty paired HNSCC and adjacent non-tumor tissues by immunohistochemistry. Immunocytochemistry, semiquantitative reverse transcription sq-PCR and quantitative real-time qPCR were used to assess protein and mRNA expressions in peripheral blood of fifty HNSCC patients and hundred controls. Results: ARG1 and ODC protein and mRNA were strongly expressed in peripheral blood from HNSCC patients. No ARG2 expression was observed. In vivo, expression of ARG1, ARG2 and ODC was significantly higher in tumor than in non-tumor tissues. Most tumors expressed low levels of OAT, with no difference in tissues or blood, compared to controls. The absolute extent of maximal ARG1 upregulation with qPCR showed 6.23 fold increase in HNSCC. Conclusions: These findings strongly suggest that in HNSCCs, the ARG1 pathway is stimulated leading to the formation of polyamines as indicated by higher ODC expression, which promote tumor growth.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.9
• /
• pp.4707-4712
• /
• 2012

Annexin A1 is a 37-kDa calcium- and phospholipid-binding protein of the annexin superfamily considered to play an important role in tumorigenesis. However, associations with clinicopathological features in pancreatic ductal adenocarcinoma (PDAC) cases have yet to be fully defined. We therefore investigated the prognostic value of annexin A1 protein as a PDAC biomarker in 83 tumor and matched non-cancerous tissues or normal pancreas tissues. Expression was analyzed using real-time RT-PCR, Western blotting and immunohistochemistry. In non-tumor tissue, myoepithelial cells showed no or weak expression of annexin A1 while expression was strong and sometimes even located in the nuclei of endothelial cells in tumor tissue. High expression was significantly associated with advanced stage (P <0.05) and a worse overall survival (P <0.05). These results provide new insights to better understand the role of annexin A1 in PDAC survival, and might be relevant to prediction of prognosis and development of more effective therapeutic strategies aimed at improving survival.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.6
• /
• pp.3631-3634
• /
• 2013

MicroRNAs (MiRNAs) play important roles in coordinating a variety of cellular processes and abnormal expression has been linked to the occurrence of several cancers. The miRNA miR-451 is downregulated in colorectal carcinoma (CRC) cells, suggested by several research groups including our own. In this study, synthetic miR-451 mimics were transfected into the SW620 human CRC cell line using Lipofectamine 2000 and expression of miR-451 was analyzed by real time PCR, while expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2 was analyzed by Western blot, and cell growth was detected by MTT assay. In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2. The capacity of cell proliferation was significantly decreased by miR-451 expression, which also inhibited cell growth. Our study confirmed that miR-451 has a repressive role in CRC cells by inhibiting cell growth through down-regulating the P13K/AKT pathway.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.12 no.6
• /
• pp.2881-2894
• /
• 2018

Cyber attacks are increasing continuously. On average about one million malicious codes appear every day, and attacks are expanding gradually to IT convergence services (e.g. vehicles and television) and social infrastructure (nuclear energy, power, water, etc.), as well as cyberspace. Analysis of large-scale cyber incidents has revealed that most attacks are started by PCs infected with malicious code. This paper proposes a method of detecting an attack IP automatically by analyzing the characteristics of the e-mail transfer path, which cannot be manipulated by the attacker. In particular, we developed a system based on the proposed model, and operated it for more than four months, and then detected 1,750,000 attack IPs by analyzing 22,570,000 spam e-mails in a commercial environment. A detected attack IP can be used to remove spam e-mails by linking it with the cyber removal system, or to block spam e-mails by linking it with the RBL(Real-time Blocking List) system. In addition, the developed system is expected to play a positive role in preventing cyber attacks, as it can detect a large number of attack IPs when linked with the portal site.

• Smart Structures and Systems
• /
• v.22 no.5
• /
• pp.509-525
• /
• 2018

Sensors and systems in Civionics technology play an important role for continuously facilitating real-time structure monitoring systems by detecting and locating damage to or degradation of structures. An advanced materials, design processes, long-term sensing ability of sensors, electromagnetic interference, sensor placement techniques, data acquisition and computation, temperature, harsh environments, and energy consumption are important issues related to sensors for structural health monitoring (SHM). This paper provides a comprehensive survey of various sensor technologies, sensor classes and sensor networks in Civionics research for existing SHM systems. The detailed classification of sensor categories, applications, networking features, ranges, sizes and energy consumptions are investigated, summarized, and tabulated along with corresponding key references. The current challenges facing typical sensors in Civionics research are illustrated with a brief discussion on the progress of SHM in future applications. The purpose of this review is to discuss all the types of sensors and systems used in SHM research to provide a sufficient background on the challenges and problems in optimizing design techniques and understanding infrastructure performance, behavior and current condition. It is observed that the most important factors determining the quality of sensors and systems and their reliability are the long-term sensing ability, data rate, types of processors, size, power consumption, operation frequency, etc. This review will hopefully lead to increased efforts toward the development of low-powered, highly efficient, high data rate, reliable sensors and systems for SHM.

• Archives of design research
• /
• v.13 no.3
• /
• pp.39-48
• /
• 2000

Under the ambiguous situation that design aim is not defined, this study would help designers with 1. overcoming the limitation of form generation ability by establishing visual application system, 2. accepting users'opinions by generating images dynamically, analysing and giving information on the preferred ones on the web on real time, 3. identifying tendency of preference so that they can generate preferred colors and images in future by updating image combination and dropping low-preferred ones. This system would play a role as an idea or form generation application in the product design development process.

• Journal of Animal Reproduction and Biotechnology
• /
• v.34 no.2
• /
• pp.111-116
• /
• 2019

This study was conducted to analyze the specific genes associated with sex-determination in Korean native cow. The highly organized spermatogenesis requires accurate spatial and temporal regulation of gene expression, which is governed by transcriptional, post-transcriptional, and epigenetic processes. Recently, farmers have been interested in determining the sexual identity of the calves in their farm. We analyzed the sperm of Korean native and Holstein cows, which were supplied from Hanwoo Improvement Center. We evaluated sperm motility and expression of sperm-specific genes after treating semen with both male- and female reagents. Sperm motility in Korean native cows decreased by approximately 10% in the first 30 minutes after treatment with sex-determination reagent. However, sperm motility of Holstein cows decreased to 60-70% after 15 minutes and to 20-30% after 30 minutes. We selected six specific genes expressing in the spermatozoa to analysis the gene expression level. The Real-time PCR results suggest that the selected genes (Gimap4, Tmeff1, Rac2, Abi2, Rac1, and Clu) were highly expressed in the group treated with the male reagent compared to the group treated the female reagent and to the untreated-group (control). In the present study, we suggest that the selected genes play a pivotal role in sex-determination.

• Journal of Biomedical Engineering Research
• /
• v.35 no.6
• /
• pp.219-226
• /
• 2014

This study is to develop a basic life support (BLS) app using the android based smartphone and to evaluate the function of the app. Suggested app contains chest compression feedback function, the map of automated external defibrillator (AED), direct emergency call and the basic knowledge of BLS. Using the accelerometer of the smartphone, we implemented a real-time algorithm that estimates the chest compression depth and rate for high quality cardiopulmonary resuscitation (CPR). The accuracy of algorithm was evaluated by manikin experiment. We made contents which were easy to learn the BLS for the layperson and implemented a function that provides the AED location information based on the user's current location. From the manikin experiment, the chest compression depth and rate were no significant differences between the manikin data and the app's feedback data (p > 0.05). Developed BLS app was uploaded on Google Play Store and it was free to download. We expected that this app is useful to learn the BLS for the layperson.

• The Journal of the Korea Contents Association
• /
• v.7 no.10
• /
• pp.27-35
• /
• 2007

Radio Frequency Identification(RFID) technology is set to play an essential role in object tracking or supply chain management systems. New challenges for RFID data management are needed in the RFID applications. RFID data are generated quickly and automatically, and can be used for object tracking, or for real-time monitoring. These applications are mostly associated with the timestamps when the events happen. In this paper, we propose a temporal RFID data model to maintain the history of events and state changes and to monitor the states of RFID objects. Also we propose data filtering method of non active data based on temporal RFID data model. This data model involves essential basic operations for RFID data. We show increased query performance through the data filtering method of non active data.

• Journal of Information Processing Systems
• /
• v.5 no.2
• /
• pp.77-86
• /
• 2009

In this paper, we propose a framework for the real-time monitoring of wireless biosensors. This is a scalable platform that requires minimum human interaction during set-up and monitoring. Its main components include a biosensor, a smart gateway to automatically set up the body area network, a mechanism for delivering data to an Internet monitoring server, and automatic data collection, profiling and feature extraction from bio-potentials. Such a system could increase the quality of life and significantly lower healthcare costs for everyone in general, and for the elderly and those with disabilities in particular.