• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.5
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• pp.1963-1978
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• 2015

With the advancement of mobile web environments, identification and analysis of the user behavior play a significant role and remains a challenging task to implement with variations observed in the model. This paper presents an efficient method for mining optimized user behavior prediction model using genetic algorithm on mobile web structure. The framework of optimized user behavior prediction model integrates the temporary and permanent register information and is stored immediately in the form of integrated logs which have higher precision and minimize the time for determining user behavior. Then by applying the temporal characteristics, suitable time interval table is obtained by segmenting the logs. The suitable time interval table that split the huge data logs is obtained using genetic algorithm. Existing cluster based temporal mobile sequential arrangement provide efficiency without bringing down the accuracy but compromise precision during the prediction of user behavior. To efficiently discover the mobile users' behavior, prediction model is associated with region and requested services, a method called optimized user behavior Prediction Model using Genetic Algorithm (PM-GA) on mobile web structure is introduced. This paper also provides a technique called MAA during the increase in the number of models related to the region and requested services are observed. Based on our analysis, we content that PM-GA provides improved performance in terms of precision, number of mobile models generated, execution time and increasing the prediction accuracy. Experiments are conducted with different parameter on real dataset in mobile web environment. Analytical and empirical result offers an efficient and effective mining and prediction of user behavior prediction model on mobile web structure.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.8
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• pp.59-66
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• 2009

Recently, as the expectation about the IPTV (Internet Protocol TV) service quality is rapidly increased by the development of the national high-speed internet and TPS (the Triple Play Service : data + image + audio) service Therefore, the enactment of the national quality standards about the IPTV service quality guaranteeing the real time video quality of a subscriber and the international standards are hastily needed. This paper built a test bed with the network domain and the subscriber set-top box domain including the headend area and commercial network characteristic in order to test in the environment which is similar to the characteristic of the service business network. And by using the constructed environment, the characteristics required for SLA(service Level Agreement) of the IPTV service are presented as the quality test according to the service environment change.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.17-24
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• 2010

In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communications and interactions between oocytes and cumulus cells. However, information on stage specific gene expression in swine during the phase is not well understood. To investigate the function of cumulus cells during in vitro maturation of porcine oocytes and gene expression, suppression subtractive hybridization (SSH) was performed to screen genes that were differentially expressed between cumulus-oocyte complexes (COCs) and naked oocytes (NOs). Utilizing mRNAs from in vitro maturation oocytes, a SSH cDNA library from COCs as the tester and NOs as the driver was constructed. The SSH cDNA library was then screened using dot blot analysis. Results showed that a total of 70 clones randomly selected from the library were differentially expressed. Among these, 41 exhibited high homology to known genes and 11 were novel expressed sequences tags (ESTs). Four differentially expressed genes, including bfgf, sprouty 2, egr and btc, were further studied by real time quantitative PCR; results confirmed an increased expression of respective mRNA in COCs compared with NOs, which suggests that these factors may play an important role in oocyte development and maturation.

• Biomedical Science Letters
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• v.18 no.2
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• pp.169-174
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• 2012

The placenta is an extraembryonic tissue that is formed between mother and fetus and mediates delivery of nutrients and oxygen from the mother to the fetus. Because of its essential role in sustaining the growth of the fetus during gestation, defects in its development and function frequently result in fetal growth retardation or intrauterine death, depending on its severity. Vertebrate Hox genes are well known transcription factors that are essential for the proper organization of the body plan during embryogenesis. However, certain Hox genes have been known to be expressed in placenta, implying that Hox genes not only play a crucial role during embryonic patterning but also play an important role in placental development. So far, there has been no report that shows the expression pattern of the whole Hox genes during placentation. In this study, therefore, we investigated the Hox gene expression pattern in mouse placenta, from day 10.5 to 18.5 of gestation using real-time RT-PCR method. In general, the 5' posterior Hox genes were expressed more in the developing placenta compared to the 3' Hox genes. Statistical analysis revealed that the expression of 15 Hox genes (Hoxa9, -a11, -a13/ -b8, -b9/ -c6, -c9, -c13/ -d1, -d3, -d8, -d9, -d10, -d11, -d12) were significantly changed in the course of gestation. The majority of these genes showed highest expression at gestational day 10.5, suggesting their possible role in the early stage during placental development.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.1
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• pp.355-362
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• 2014

Objective: Altered regulation of many transcription factors has been shown to play important roles in the development of leukemia. hMSH2 can modulate the activity of some important transcription factors and is known to be a regulator of hematopoietic differentiation. Herein, we investigated epigenetic regulation of hMSH2 and its influence on cell growth and overall survival of acute lymphoblastic leukemia (ALL) patients. Methods: hMSH2 promoter methylation status was assessed by COBRA and pyrosequencing in 60 ALL patients and 30 healthy volunteers. mRNA and protein expression levels of hMSH2, PCNA, CyclinD1, Bcl-2 and Bax were determined by real time PCR and Western blotting, respectively. The influence of hMSH2 on cell proliferation and survival was assessed in transient and stable expression systems. Results: mRNA and protein expression of hMSH2 and Bcl-2 was decreased, and that of PCNA, CyclinD1 and Bax was increased in ALL patients as compared to healthy volunteers (P<0.05). hMSH2 was inactivated in ALL patients through promoter hypermethylation. Furthermore, hMSH2 hypermethylation was found in relapsed ALL patients (85.7% of all cases). The median survival of patients with hMSH2 methylation was shorter than that of patients without hMSH2 methylation (log-rank test, P=0.0035). Over-expression of hMSH2 in cell lines resulted in a significant reduction in growth and induction of apoptosis. Conclusions: This study suggests that aberrant DNA methylation and epigenetic inactivation of hMSH2 play an important role in the development of ALL through altering cell growth and survival.

• Child Health Nursing Research
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• v.4 no.2
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• pp.255-264
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• 1998

Disease prevention and health promotions are basically needed to be healthy, health education is the most useful mean to accomplish them. Preschool children are being developped, their health problem can effect their health status through their lives. This study was attempted to survey for status of health education of kindergarten for preshoolers and to provide the basic precious data for nursing intervention. The subjects were 51 kidergarten teachers. The data were collected by the questionaires, which consiste of 25 items concerning daily life habits of children and 1 item concerning the problems of health education for preschoolers. The data were analyzed by SAS program. The results of this study are as folleowed ; 1. The most frequently educated subjects were 'adequate and safe play' and 'hand washing', 'walking and running straightly', 'oral hygiene or tooth brushing', 'walking staires'. 2. The most frequently used teaching method was the explaining. 3. The most frequently used instructional method was the pictures. The slide films and OHP was seldomly used media. 4. The most effective teaching method which perceived by the kindergarten teachers was role play . Discuss ion and compensation/reinforcement were comparatively effective method also. 5. The most effective instructional media which perceived by the kindergarten teachers was a real object/model. 6. The problems of health education for preschoolers were 'deficiency of developped health educational programs' and 'inconsistency between parents' education and kindergarten teachers' education', 'deficiency of instructional media'. The other problems were' the knowledge deficit of teachers themselves' and 'the difficulty of measure the effect of education', 'time deficit'. Conclusionally, we have to realize the health of children is the future of our health, and reinforce the health education for preschoolers to accomplish the disease prevention and health promotion.

• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.93-102
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• 2012

This study started from the project that make the record of legendary actor's heritage in Korea. This research was focused on Sung Hee Beck's artistic footprint which was almost same steps as the Korean modern play of the centennial theater history by reviewing her; domestic royal actor; acting techniques which learn from real acting field by herself and her career. Her life shows constant resistance for the social negative reputation of the acting field, and her frantic effort to Korean play and actor's phase. Above all, this passionate actor's life contributes to society, and improve the Korean theater through 70 year's her experiences with countless roles in around 400 performances and her own valuable acting techniques. To look up legendary actress of our time, Sung Hee Beak's life gives us a chance to reflect art of acting which emphasizes its fundamental.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1651-1655
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• 2015

UGT1A play important roles in the glucuronidation of a variety of endogenous and exogenous compounds. UGT1A isoforms are expressed tissue specifically. The aim of this study was to examine the relationship between UGT1A3 and UGT1A7 mRNA expression and pancreatic cancer. Paired healthy and tumor tissue samples of 43 patients with pancreatic cancer were included in this study. UGT1A3 and UGT1A7 mRNA expressions were analyzed by real time-PCR. In the result of study, UGT1A3 and UGT1A7 mRNA expressions were significantly higher in tumor tissue than normal tissue of pancreatic cancer patients (p<0.05). In addition, high mRNA expression of UGT1A3 and UGT1A7 was significantly associated with larger tumor size (p<0.05). The data suggested that UGT1A3 and UGT1A7 may play roles in the progression of pancreatic cancer. Consequently, UGT1A3 and UGT1A7 are potential prognostic indicators.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.5281-5286
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• 2012

Purpose: Receptor 7 (CXCR7) has recently been characterized as a novel receptor for CXCL12/SDF-1 (stromal cell derived factor-1). Given the demonstrated importance of CXCL12/SDF-1 in angiogenesis and tumour metastasis, we hypothesized that CXCR7 may also play a role in tumour pathogenesis. Located in the limited space of the intracranial cavity, any brain tumours can be inherently serious and life-threatening. However, the expression of CXCR7 in pituitary adenoma, neurilemmoma or hemangioblastoma remains to be elucidated. Therefore, we aimed to determine the potential contribution of CXCR7 in the development of brain tumours. Methods: In this study we examined and quantified the mRNA expression of CXCR7 in four different human brain tumours - 27 patients with neurilemmoma (8 patients), pituitary adenoma (7 patients), hemangioblastoma (6 patients), or meningioma (6 patients) undergoing surgical resection in the West China Hospital of Sichuan University. There were 15 females and 12 males aged from 28 to 70 years old. Total RNA was isolated and mRNA was measured by quantitative real-time RT-PCR. One-way analysis of variance (ANOVA) was performed using SPSS 11.0 statistical software to compare the mRNA levels of CXCR7 among four groups. Results: We found that CXCR7 mRNA was detected in all tumour samples. Quantitative results showed that the levels of CXCR7 mRNA in brain tissues from patients with neurilemmoma or meningioma were significantly higher than those with pituitary adenoma or hemangioblastoma. Conclusions: The results suggest that the CXCR7 may play a role in progression, metastasis and angiogenesis of brain tumours.

• Journal of Korea Multimedia Society
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• v.6 no.6
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• pp.1015-1024
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• 2003

With the improvement of the computer performance, the users demand for the multimedia player is getting higher, which will be the major force behind the wide distribution of the media player that allows several moving pictures to run simultaneously using multiple windows on the screen. Products that adopted the same method used for a single moving picture and yet support the several moving pictures simultaneously emerged; however, they are not immune from problems. In this paper, we propose method to solve the problems of blinking, play prioritization processor and semi-transparent Processor that occur when appling several moving pictures to the method used for a single moving picture and suggested the possible solutions. Taking it one step further, it focused on design and implementation of the media player that allows several moving pictures using overlapping and overlay technique by recognizing the biggest problem, which is that the above media play is not supported when playing several moving pictures simultaneously.