• The Plant Pathology Journal
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• 제27권4호
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• pp.385-389
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• 2011

A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RT-LAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RT-LAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

• Journal of Multimedia Information System
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• 제1권1호
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• pp.77-85
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• 2014

Usually, the skin pigmentation detection and diagnosis are made by clinicians. In this process it is subjective and non-quantitative. We develop an approach to detect and measure the different pigmentation lesions base on computer vision technology. In the paper we study several usually used skin-detecting color space like HSV, YCbCr and normalized RGB. We compare their performance with illumination influence for detecting the pigmentation lesions better. Base on a relatively stable color space, we propose an approach which is RGB channels vector difference characteristic for the detection. After the object region detection, we also use the difference to measure the difference between the lesion and the surrounding normal skin. From the experiment results, our approach can effectively detect the pigmentation lesion, and perform robustness with different illumination.

• 한국안전학회지
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• 제20권3호
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• pp.1-8
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• 2005

The industrial induction motor is widely used in the rotating electrical machine for the transmission of power. It is very reliable equipment, but it could lead to the loss of production and lift when failure occurs. Therefore, the failure data is acquired and analyzed by attaching an exclusive instrument to existing induction motor. However, these instruments could lead to side effects, increasing the production costs, because they are very expensive. The purpose of this study is the development of an induction motor bearing failure diagnosis system constructed using LabVIEW which can be supplied the kernelled function, process monitoring and current signature analysis. In addition, the availability and reasonability of the constructed system was examined for an induction motor with failure defects in outer raceway and ball bearing. From the results, it shows that failure diagnosis system constructed is useful for real-time monitoring with detection of bearing defects over the web.

• 한국자동차공학회논문집
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• 제16권4호
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• pp.173-178
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• 2008

Electro-hydraulic brake system has many advantages. It provides improved braking performance and stability functions. It also removes complex mechanical parts for freedom of design, improves maintenance requirements and reduces unit weight. However, the EHB system should be dependable and have back-up redundancy in case of a failure. In this paper, the model-based fault diagnosis system is developed to monitor the brake status using the analytical redundancy method. The performance of the model-based fault diagnosis system is verified in real-time simulation. It demonstrates the effectiveness of the proposed system in various faulty cases.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회B
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• pp.2070-2075
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• 2008

Identification of steady-state is the first step in developing a fault detection and diagnosis (FDD) system. In a complete FDD system, the steady-state detector will be included as a module in a self-learning algorithm which enables the working system's reference model to "tune" itself to its particular installation. In this study, a steady-state detector of a residential air conditioner based on moving windows was designed. Seven representing measurements were selected as key features for steady-state detection. The optimized moving window size and the feature thresholds was suggested through startup transient test and no-fault steady-state test. Performance of the steady-state detector was verified during indoor load change test. From the research, the general methodology to design a moving window steady-state detector was provided for vapor compression applications.

• 항공우주기술
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• 제11권2호
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• pp.182-194
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• 2012

한정된 기간과 비용 하에 높은 신뢰도와 안전성을 갖는 엔진을 완성하기 위해서는 엔진 개발과 병행하여 엔진에 최적화된 진단시스템의 개발이 필요하다. 본 연구에서는 진단시스템의 개발방향을 정립하기 위하여 해외문헌을 바탕으로 엔진에서 발생 가능한 고장들, 상태진단을 위한 검사파라미터의 특성, 진단방법들(실시간 진단법, 사후 진단법, 사고원인 분석법, 파라미터 계통법, 시험진단법)을 고찰하였고, 엔진 개발단계 및 운용단계에서 수행해야할 진단관련 과제들을 제시하였으며, 해외의 액체로켓엔진 진단 사례를 정리하였다.

• 대한의생명과학회지
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• 제22권1호
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• pp.24-28
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• 2016

Tuberculosis (TB) remains an unsolved community health problem since identification of its causing microorganism called Mycobacterium tuberculosis (MTB) by Robert Koch in 1882. Annually, eight million TB cases are newly reported and 2~3 million patients die from TB. Pulmonary TB is highly infectious and untreated pulmonary TB patients are believed to infect >10 people in a year. The conventional methods for diagnosis of TB are chest X-ray and isolation of the causing microorganisms from patient specimens. Screening of TB is conducted with smeared sputum in slides, and TB is confirmed by identification of MTB in cultured specimens. One of the fatal pitfalls of screening detection for smeared sputum is that it is impossible to distinguish MTB and other acid-fast bacilli (AFB) because they are stained equally with Ziehl-Neelsen (ZN) stain. Culture of MTB is the most reliable method for diagnosis of TB but it takes 4~8 weeks. In this report, we suggest a fast and highly-reliable MTB detection method that distinguishes AFB in sputum samples. Purified DNA from the AFB stained slide samples offered by The Korean Institute of Tuberculosis were used to detect infected MTB in patients. PCR, real-time PCR and reverse blot hybridization assay (REBA) methods were applied to purified DNA. Conclusively, the real-time PCR method was confirmed to produce high sensitivity and we were able to further detect drug-resistant MTB with REBA.

• 비파괴검사학회지
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• 제35권3호
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• pp.185-192
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• 2015

동적하중에서의 베어링 결함에 대한 실시간 진단기술은 상대적으로 저조하다. 따라서 볼베어링의 이상상태 현상으로 인한 온도 상승 및 진동 증가 등을 사전에 검출하는 기술이 필요하며, 회전체에 대한 운전상태 감시 및 손상 진단을 통해 발전설비의 원활한 운전을 기할 수 있는 검출 기술이 필요하다. 적외선 열화상 실험과 더불어 ANSYS를 이용한 유한요소해석으로부터 실험과 동일한 베어링을 구조 설계 및 해석하여 데이터를 분석함으로써 열화상 기술로 얻은 데이터의 신뢰성을 확보하였다.

• Parasites, Hosts and Diseases
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• 제48권2호
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• pp.99-103
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• 2010

Malaria is endemic in the Cukurova region while it is sporadic in other regions of Turkey. Therefore, the laboratory and clinical diagnosis of malaria is important for the treatment of malaria. In this study, 92 blood samples that were taken from the suspected malaria patients for routine diagnosis in a period of 10 years between 1999 and 2009 were analyzed. All of these blood samples were examined by microscopic examinations using Giemsa-stained thick blood films, nested PCR, and real-time PCR. The sensitivity-specificity and positive-negative predictive values for these diagnostic tests were then calculated. It was found that the positive predictive values of microscopic examination of thick blood films, nested PCR, and real-time PCR were 47.8%, 56.5%, and 60.9% for malaria, respectively. The real-time PCR was found to have a specificity of 75% and sensitivity of 100%, while specificity and sensitivity of nested PCR was found 81.2% and 97.7% according to the microscopic examination of thick blood films, respectively.

• 대한의생명과학회지
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• 제26권4호
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• pp.302-309
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• 2020

Human Coxsackievirus B5 (HuCoxV-B5) infection has been associated with various diseases such as myocarditis, aseptic meningitis, hand-foot-and mouth-disease, and insulin-dependent diabetes. HuCoxV-B5 is a virus transmitted through the fecal-oral route and is detected in clinics, aquatic environments, food, shellfish, etc. and is one of the more important viruses in public health because of its incidence rate reported worldwide. In this study, a combination of SYBR Green-based real-time PCR primers for molecular diagnosis including monitoring of HuCoxV-B5 was selected and the optimal reaction conditions were established. Compared with the previously reported TaqMan probe-based real-time PCR method, assessments including a sample applicability test were performed. Results showed that the real-time PCR method developed in this study was suitable for a molecular diagnostic technique for detecting HuCoxV-B5. This study is expected to contribute to efforts in responding to safety accidents in public health because the proposed method facilitates rapid diagnosis of clinical patients. It can also be used as a specific monitoring tool of HuCoxV-B5 in non-clinical areas such as aquatic environments among others.