• Korean Journal of Clinical Laboratory Science
• /
• v.41 no.1
• /
• pp.11-23
• /
• 2009

Chromosomal rearrangements are major pathology in hematological malignancies. The detection of minimal residual disease (MRD) for these gene rearrangements helps in monitoring treatment outcomes and predicting prognosis of patients. Recently, quantification of these gene transcripts based on real-time quantitative polymerase chain reaction (RQ-PCR) has been used as MRD detection. The purpose of this study is to ensure the usefulness of the RQ-PCR technique for detecting MRD in hamatological malignancy patients. The patients had been diagnosed to AML1-ETO positive AML, PML-RARa positive AML and BCR-ABL positive MPN at Chonnam National University Hwasun Hospital from Jan. 2006 to Aug. 2008. The fusion transcript was quntified by RQ-PCR and analyzed in comparison to conventional cytogenetics, FISH and RT-PCR. The fusion gene transcript was quantified by RQ-PCR in 57 samples from 14 patients with AML1-ETO positive AML, 79 samples from 27 patients with PML-RARa positive AML and 108 samples from 36 patients with CML. At diagnosis, the quantitative fusion transcripts for AM1-ETO, PML-RARa and BCR-ABL showed the range of 0.485552651~10.82233683 (mean 3.782217131, SD 2.998052348), 0.005300395~0.29267494 (mean 0.056901315, SD 0.080131381) and 0.1293929~12.94826849 (mean 1.701935665, SD 2.200913158). The increase of AML1-ETO fusion gene transcripts preceded morphologic relapse in two patients. Quantification of fusion gene transcripts by RQ-PCR could detected MRD in samples which were negative by in cytogenetic analysis or FISH. Our findings indicated that quantitative analysis of AML1-ETO, PML-RARa and BCR-ABL transcripts by RQ-PCR might be a useful tool for the monitoring of minimal residual disease in hematological malignancies.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.23 no.1
• /
• pp.32-37
• /
• 2011

The present study has been conducted to propose an algorithm regarding real-time load estimation of a gas engine-driven heat pump. In the study, thermal load of an indoor unit is estimated in terms of air-side and refrigerant-side. The air-side estimation is based on a typical heat exchanger model and is found to be in good agreement with experimental data. When it comes to the refrigerant-side load, a pressure difference across a valve must be estimated. For the estimation, it is assumed to be proportional to a bigger pressure difference that is available either by measurement or by estimation. Relative good agreement between the air- and refrigerant-sides suggests that the assumption may be plausible for the load estimation. The summed flow rate of all of indoor units is in good agreement with the throughput of the compressor which are calculated from the manufacturer's software. Accordingly, estimated thermal loads are also in good agreement. The proposed algorithm may be further developed for improved control algorithm and fault diagnosis.

• Korean Journal of Clinical Laboratory Science
• /
• v.54 no.3
• /
• pp.179-191
• /
• 2022

Carbapenem-resistant Enterobacteriaceae (CRE) poses an increasing public health threat and has limited treatment options with high associated mortality. Genotypes of carbapenemase that threaten public health (bla_KPC, bla_NDM, bla_IMP, and bla_VIM) and bla_OXA-48-like genes were detected by phenotypic and molecular diagnosis, and related gene distribution patterns were investigated. Phenotypic testing using the modified Hodge test confirmed positivity in all 41 strains examined, and carbapenemase inhibitory testing using meropenem+phenyl boronic acid or meropenem+EDTA confirmed positivity in 18 and 8 strains, respectively. Polymerase chain reaction revealed the presence of amplification products in 28 strains of bla_KPC, 25 strains of bla_NDM, 5 strains of bla_IMP, 1 strain of bla_VIM, and 13 bla_OXA-48-like strains. In addition, 7 strains of bla_KPC+bla_NDM, 1 strain of bla_KPC+bla_IMP, 1 strain of bla_NDM+bla_OXA-48-like, 1 strain of bla_NDM+bla_VIM, 4 strains of bla_KPC+bla_NDM+bla_IMP, and 4 strains of bla_KPC+bla_NDM+bla_OXA-48-like were identified. Melting curve analysis using real-time PCR was wholly consistent with PCR results. The study shows genetic identification of highly specific CRE by real-time PCR could be used to provide early diagnoses and infection control, improve surveillance, and prevent the transmission of CRE.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.5
• /
• pp.2945-2948
• /
• 2013

Background: Mouse mammary tumor virus (MMTV) is the major cause of mammary tumors in mice. There is limited controversial evidence about the probable etiologic role of MMTV- like virus in human breast cancer. Materials and Methods: A total of 40 Formalin fixed paraffin embedded samples with diagnosis of breast cancer were collected in a period of 3 years from cancer institute of Iran. We selected both pre-menopausal and post-menopausal patients with different histologic grades and different ethnic groups. We evaluated presence of MMTV-like virus env gene through real time PCR method. Results: Forty patients (20 pre and 20 postmenopausal women) were evaluated with the mean age of 49.67. The average tumor size was 39 mm. None of the studied samples were positive for MMTV-like virus env gene target sequences. Conclusions: We found no evidence on the potential role of MMTV-like virus in the carcinogenicity of breast cancer among Iranian women.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.23 no.1
• /
• pp.35-39
• /
• 2019

Purpose Hepatitis B virus (hepatitis B virus, HBV) infection is a worldwide major public health problem and it is known as a major cause of chronic hepatitis, liver cirrhosis and liver cancer. And serologic tests of hepatitis B virus is essential for diagnosing and treating these diseases. In addition, with the development of molecular diagnostics, the detection of HBV DNA in serum diagnoses HBV infection and is recognized as an important indicator for the antiviral agent treatment response assessment. We performed HBeAg assay using Immunoradiometric assay (IRMA) and Chemiluminescent Microparticle Immunoassay (CMIA) in hepatitis B patients treated with antiviral agents. The detection rate of HBV DNA in serum was measured and compared by RT-PCR (Real Time - Polymerase Chain Reaction) method Materials and Methods HBeAg serum examination and HBV DNA quantification test were conducted on 270 hepatitis B patients undergoing anti-virus treatment after diagnosis of hepatitis B virus infection. Two serologic tests (IRMA, CMIA) with different detection principles were applied for the HBeAg serum test. Serum HBV DNA was quantitatively measured by real-time polymerase chain reaction (RT-PCR) using the Abbott m2000 System. Results The detection rate of HBeAg was 24.1% (65/270) for IRMA and 82.2% (222/270) for CMIA. Detection rate of serum HBV DNA by real-time RT-PCR is 29.3% (79/270). The measured amount of serum HBV DNA concentration is $4.8{\times}10^7{\pm}1.9{\times}10^8IU/mL$($mean{\pm}SD$). The minimum value is 16IU/mL, the maximum value is $1.0{\times}10^9IU/mL$, and the reference value for quantitative detection limit is 15IU/mL. The detection rates and concentrations of HBV DNA by group according to the results of HBeAg serological (IRMA, CMIA)tests were as follows. 1) Group I (IRMA negative, CMIA positive, N = 169), HBV DNA detection rate of 17.7% (30/169), $6.8{\times}10^5{\pm}1.9{\times}10^6IU/mL$ 2) Group II (IRMA positive, CMIA positive, N = 53), HBV DNA detection rate 62.3% (33/53), $1.1{\times}10^8{\pm}2.8{\times}10^8IU/mL$ 3) Group III (IRMA negative, CMIA negative, N = 36), HBV DNA detection rate 36.1% (13/36), $3.0{\times}10^5{\pm}1.1{\times}10^6IU/mL$ 4) Group IV(IRMA positive, CMIA negative, N = 12), HBV DNA detection rate 25% (3/12), $1.3{\times}10^3{\pm}1.1{\times}10^3IU/mL$ Conclusion HBeAg detection rate according to the serological test showed a large difference. This difference is considered for a number of reasons such as characteristics of the Ab used for assay kit and epitope, HBV of genotype. Detection rate and the concentration of the group-specific HBV DNA classified serologic results confirmed the high detection rate and the concentration in Group II (IRMA-positive, CMIA positive, N = 53).

• Journal of Gastric Cancer
• /
• v.17 no.1
• /
• pp.43-51
• /
• 2017

Purpose: This study aimed to analyze G3BP1 and VEZT expression profiles in patients with gastric cancer, and examine the possible relationship between the expressions of each gene and clinicopathological factors. Materials and Methods: Expression of these genes in formalin-fixed paraffin embedded (FFPE) tissues, collected from 40 patients with gastric cancer and 40 healthy controls, was analyzed. Differences in gene expression among patient and normal samples were identified using the GraphPad Prism 5 software. For the analysis of real-time polymerase chain reaction products, GelQuantNET software was used. Results: Our findings demonstrated that both VEZT and G3BP1 mRNA expression levels were downregulated in gastric cancer samples compared with those in the normal controls. No significant relationship was found between the expression of these genes and gender (P-value, 0.4835 vs. 0.6350), but there were significant changes associated with age (P-value, 0.0004 vs. 0.0001) and stage of disease (P-value, 0.0019 vs. 0.0001). In addition, there was a direct relationship between VEZT gene expression and metastasis (P-value, 0.0462), in contrast to G3BP1 that did not demonstrate any significant correlation (P-value, 0.1833). Conclusions: The results suggest that expression profiling of VEZT and G3BP1 can be used for diagnosis of gastric cancer, and specifically, VEZT gene could be considered as a biomarker for the detection of gastric cancer progression.

• Clinics in Shoulder and Elbow
• /
• v.18 no.3
• /
• pp.172-193
• /
• 2015

Nowadays shoulder ultrasound is commonly used in the assessment of shoulder diseases and is as accurate as magnetic resonance imaging in the detection of several pathologies. Operator dependence is the main disadvantage of shoulder ultrasound. After adhering to a strict examination protocol, good knowledge of normal anatomy and pathologic processes and an awareness of common pitfalls, it can be used as a focused examination providing rapid, real-time diagnosis, and treatment by ultrasound-guided interventions in desired clinical situations. Also shoulder ultrasound can help the surgeon decide whether treatment will be surgical or nonsurgical. If arthroscopy is planned, sonographic findings help to counsel patients regarding surgical and functional outcomes. If a nonsurgical approach is indicated, ultrasound can be used to follow patients. This review article presents the examination techniques, the normal sonographic appearances and the main pathologic conditions found in shoulder ultrasound. And also addresses a simplified approach to scanning and ultrasound-guided intervention. Knowledge of optimal techniques, normal anatomy, dynamic maneuvers, and pathologic conditions is essential for optimal performance and interpretation of images.

• KIEE International Transactions on Power Engineering
• /
• v.4A no.4
• /
• pp.214-220
• /
• 2004

Power quality monitoring is the cornerstone for power quality analysis, diagnosis and improvement. The measurement of power quality (PQ) evolves from instantaneous metering to continuous monitoring. Furthermore, recent technologies enable us to construct more flexible, reliable, rapid and economical power quality monitoring system (PQMS). Therefore, this paper presents an improved PQMS with a new distributed monitoring structure. The proposed PQMS consists of a PQ meter, PQ analyzer and GUI. The PQ meter only collects raw data and the PQ analyzer performs power quality analysis. It has several advantages compared to conventional structures in economic efficiency, modularity, speed, etc. PQ monitoring algorithms to catch steady-state trends and to detect PQ events are also adapted to the proposed structure. Using the proposed structure and monitoring algorithm, a prototype PQMS is constructed and real-time testing is performed.

• Journal of Korea Society of Industrial Information Systems
• /
• v.11 no.1
• /
• pp.69-76
• /
• 2006

Telematics, which is a compound word of a telecommunication and informatics, provides drivers with useful driving information such as driving path guidance, accident or robbery detection, traffic conditions and other valuable data at real time. This paper proposes and implements how to build a telematics terminal equipped with CDMA and GPS running embedded Linux, to check a vehicle's state through communication between telematics server and vehicle terminals using a cellular phone and to control a vehicle using SMS as shown in Figure 1. In order to do this, we use the SK-VM platform which is mobile terminal platform based on JAVA to design, implement and evaluate it.

• Journal of Electrical Engineering and Technology
• /
• v.11 no.3
• /
• pp.683-689
• /
• 2016

Most outdoor solid insulators may suffer from surface degradations due to non-stationary currents that flow on the insulator surface. These currents may be classified as leakage, discharge and tracking currents due to their disturbing potencies respectively. The magnitude of these currents depends on the degree of the contamination of surface. The leakage signals are followed by discharge signals and tracking signals which are capable of forming carbonized tracking paths on the surface between high voltage and earth contacts (surface tracking). Surface tracking is one of the most breakdown mechanisms observed on the solid insulators, especially polymers which may cause severely reduced lifetime. In this study the degradations observed on polyester resin based insulators are investigated according to the IEC 587 Inclined Plane Test Standard. The signals are monitored and recorded during tests until surface tracking initiated. In order to prevent total breakdown of an insulator, early detection of tracking signals is vital. Continuous Wavelet Transform (CWT) is proposed for classification of signals and their energy levels observed on the surface. The application of CWT for processing and classification of the surface signals which are prone to display high frequency oscillations can facilitate real time monitoring of the system for diagnosis.