• Title/Summary/Keyword: Real-time TEM

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Determination of an Underground Seawater Flow Using a TEM Decay Curve (TEM감쇠곡선을 이용한 해수의 지하 유동현상 파악)

  • 황학수;문창규;이상규;이태섭
    • Economic and Environmental Geology
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    • v.34 no.5
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    • pp.499-506
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    • 2001
  • The geophysical monitoring technique using the high resolution time-domain electromagnetic (TEM) method with a coincident loop away was applied for determination of an underground seawater flow in the coastal areas. In comparison of the TEM monitoring to direct current (DC) resistivity monitoring, the TEM response to the under ground seawater flow is less sensitive than the DC resistivity response. However, the TEM monitoring is more effective in terms of measuring time, survey expense, and real-time data processing than the DC monitoring thor evaluating the spatial distribution of the fresh water-seawater transition zone in a regional area.

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VirtualDub as a Useful Program for Video Recording in Real-time TEM Analysis (실시간 TEM 분석에 유용한 영상 기록 프로그램, VirtualDub)

  • Kim, Jin-Gyu;Oh, Sang-Ho;Song, Kyung;Yoo, Seung-Jo;Kim, Young-Min
    • Applied Microscopy
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    • v.40 no.1
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    • pp.47-51
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    • 2010
  • The capability of real-time observation in TEM is quite useful to study dynamic phenomena of materials in a certain variable ambience. In performing the experiment, the choice of video recording program is an important factor to obtain high quality of movie streaming. Window Movie Maker (WMM) is generally recommended as a default video recording program if one uses "DV Capture" function in DigitalMicrograph$^{TM}$ (DM) software. However, the image quality does not often satisfy the condition for high-resolution microscopic analysis since the severe information loss in the final result occurs during the conversion process. As a good candidate to overcome this problem, Virtual-Dub is highly recommended since the information loss can be minimized through the streaming process. In this report, we demonstrated how useful VirtualDub works in a high-resolution movie recording. Quantitative comparison of the information quality between the images recorded by each software, WMM and VirtualDub, was carried out based on histogram analysis. As a result, the image recorded by VirtualDub was improved ~13% in brightness and ~122% in contrast compared with the image obtained by WMM at the same imaging condition. Remarkably, the gray gradation (meaning an amount of information) becomes wider up to ~115% than that of the WMM result.

Real-Time PCR Analysis of SHV Extended-Spectrum beta-Lactamases Producing Klebsiella pneumoniae (SHV ESBL생성 Klebsiella pneumoniae 균주의 실시간중합효소반응분석)

  • Yang, Byoung-Seon;Yook, Keun-Dol
    • Korean Journal of Clinical Laboratory Science
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    • v.41 no.4
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    • pp.153-157
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    • 2009
  • The production of extended-spectrum ${\beta}$-lactamases ($ESBL_S$) of the TEM or SHV type by bacterial pathogens is a major threat to the use of the clinically important expanded-spectrum cephalosporins. The characterization of the SHV ESBLs producing Klebsiella pneumoniae strains present in clinical isolates is time-consuming processes. We describe here in the development of a novel system, which consists of a real time PCR. We found 11 K. pneumoniae strains to be presumptive strains ESBLs producers by clinical and laboratory standards institute (CLSI) guidelines. The double disk synergy test showed 8 ESBL positive and conventional PCR showed 10 SHV ESBL positive, which were K. pneumoniae strains isolates. By real time PCR analysis, SHV gene in 11 of 11 strains were identified. When sequencing analysis was compared with real time PCR, both analysis were presented 99% similarity. In this study, we used a rapid, sensitive, and specific real-time PCR (RT-PCR) method for detection of the assay SHV ESBL producing K. pneumoniae strains in clinical isolates.

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Real-time Transformation of FePt Nanoparticles to L10 Phase by the Gas Phase Synthesis (기상합성공정을 이용한 FePt 나노입자의 실시간 L10 상변화)

  • Lee, Ki-Woo;Lee, Chang-Woo;Kim, Soon-Gil;Lee, Jai-Sung
    • Korean Journal of Metals and Materials
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    • v.49 no.1
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    • pp.46-51
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    • 2011
  • Real-time formation of $L1_0$ phase of FePt nanoparticles in the gas phase during ultrasonic-spray pyrolysis is first discussed in the present study. Without any post heat treatment, $L1_0$ phase of FePt nanoparticles appeared at the temperature above $900^{\circ}C$ in the gas phase synthesis. X-ray diffractometry (XRD) and transmission electron microscopy (TEM) studies revealed that FePt nanoparticles less than 10 nm in size contained small volume of $L1_0$ fct phase. However, in other samples obtained at the temperature below $900^{\circ}C$, iron oxide phase co-existed and no evidence of phase transformation was found. Thus, it is anticipated that the time of flight of particles required for crystallization and phase transformation was extended according to the increase of the collision rate. Finally, magnetic properties represented by coercivity and saturation magnetization and functional groups on the particle surface were discussed based on VSM and FT-IR results.

Investigation of Electron Thermally Induced Phase Transition in MAPbI3 Perovskite Solar Cells Using In-Situ XRD and TEM (실시간 XRD와 TEM을 이용한 MAPbI3의 온도 변화에 따른 구조 분석)

  • Choi, Jin-Seok;Eom, Ji-Ho;Yoon, Soon-Gil
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.32 no.1
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    • pp.64-69
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    • 2019
  • Methylammonium lead triiodide ($MAPbI_3$)-based perovskite solar cells potentially have potential advantages such as high efficiency and low-cost manufacturing procedures. However, $MAPbI_3$ is structurally unstable and has low phase-change temperatures ($30^{\circ}C$ and $130^{\circ}C$); it is necessary to solve these problems. We investigated the crystal structure and phase separation using real-time temperature-change X-ray diffraction, transmission electron microscopy, and electron energy loss spectroscopy. $MAPbI_3$ has a tetragonal structure, and at about $35^{\circ}C$ the c-axis contracts, transforming $MAPbI_3$ into the related cubic crystal structure. In addition, at $130^{\circ}C$, phase separation occurs in which $CH_3NH_2$ and HI at the center of the unit cell of the perovskite structure are extracted by gas, leavingand only $PbI_2$ of the three-component structure, is produced as the final solid product.

In-situ HRTEM Studies of Alumina-Aluminum Solid-Liquid Interfaces

  • Oh, Sang-Ho;Scheu, Christina;Ruhle, Manfred
    • Applied Microscopy
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    • v.36 no.spc1
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    • pp.19-24
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    • 2006
  • The alumina-aluminum solid-liquid interfaces were directly observed at atomic scale by heating the alumina single crystal in high-voltage electron microscope (HVEM) owing to the electron beam damage processes, Atomic ordering in the first several layers of the liquid was clearly resolved adjacent to the alumina surface and its relevance to the single crystal growth was examined with the real-time observations.

In situ UHV TEM studies on nanobubbles in graphene liquid cells

  • Shin, Dongha;Park, Jong Bo;Kim, Yong-Jin;Kim, Sang Jin;Kang, Jin Hyoun;Lee, Bora;Cho, Sung-Pyo;Novoselov, Konstantin S.;Hong, Byung Hee
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.102-102
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    • 2016
  • Water, which is most abundant in Earth surface and very closely related to all forms of living organisms, has a simple molecular structure but exhibits very unique physical and chemical properties. Even though tremendous effort has been paid to understand this nature's core substance, there amazingly still lefts much room for scientist to explore its novel behaviors. Especially, as the scale goes down to nano-regime, water shows extraordinary properties that are not observable in bulk state. One of such interesting features is the formation of nanoscale bubbles showing unusual long-term stability. Nanobubbles can be spontaneously formed in water on hydrophobic surface or by decompression of gas-saturated liquid. In addition, the nanobubbles can be generated during electrochemical reaction at normal hydrogen electrode (NHE), which possibly distorts the standard reduction potential at NHE as the surface nanobubble screens the reaction with electrolyte solution. However, the real-time evolution of these nanobubbles has been hardly studied owing to the lack of proper imaging tools in liquid phase at nanoscale. Here we demonstrate, for the first time, that the behaviors of nanobubbles can be visualized by in situ transmission electron microscope (TEM), utilizing graphene as liquid cell membrane. The results indicate that there is a critical radius that determines the long-term stability of nanobubbles. In addition, we find two different pathways of nanobubble growth: i) Ostwald ripening of large and small nanobubbles and ii) coalescence of similar-sized nanobubbles. We also observe that the nucleation and growth of nanoparticles and the self-assembly of biomolecules are catalyzed at the nanobubble interface. Our finding is expected to provide a deeper insight to understand unusual chemical, biological and environmental phenomena where nanoscale gas-state is involved.

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Towards remote sensing of sediment thickness and depth to bedrock in shallow seawater using airborne TEM (항공 TEM 을 이용한 천해지역에서의 퇴적층 두께 및 기반암 심도 원격탐사에 관하여)

  • Vrbancich, Julian;Fullagar, Peter K.
    • Geophysics and Geophysical Exploration
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    • v.10 no.1
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    • pp.77-88
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    • 2007
  • Following a successful bathymetric mapping demonstration in a previous study, the potential of airborne EM for seafloor characterisation has been investigated. The sediment thickness inferred from 1D inversion of helicopter-borne time-domain electromagnetic (TEM) data has been compared with estimates based on marine seismic studies. Generally, the two estimates of sediment thickness, and hence depth to resistive bedrock, were in reasonable agreement when the seawater was ${\sim}20\;m$ deep and the sediment was less than ${\sim}40\;m$ thick. Inversion of noisy synthetic data showed that recovered models closely resemble the true models, even when the starting model is dissimilar to the true model, in keeping with the uniqueness theorem for EM soundings. The standard deviations associated with shallow seawater depths inferred from noisy synthetic data are about ${\pm}5\;%$ of depth, comparable with the errors of approximately ${\pm}1\;m$ arising during inversion of real data. The corresponding uncertainty in depth-to-bedrock estimates, based on synthetic data inversion, is of order of ${\pm}10\;%$. The mean inverted depths of both seawater and sediment inferred from noisy synthetic data are accurate to ${\sim}1\;m$, illustrating the improvement in accuracy resulting from stacking. It is concluded that a carefully calibrated airborne TEM system has potential for surveying sediment thickness and bedrock topography, and for characterising seafloor resistivity in shallow coastal waters.

Analysis on the Use Behavioral Patterns and Use Fluctuation over the Tong-Ch′on Amusement Park (동촌유원지의 이용실태 및 변동분석)

  • 김용수;임원현
    • Journal of the Korean Institute of Landscape Architecture
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    • v.15 no.1
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    • pp.17-37
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    • 1987
  • The purpose of this study is to establish more rational and practical planning theory for amusement park. It analyze and consider the fluctuation of people who come and use the Tong-Ch'on amusement park. The results drawn from this reserch work are as follows; 1. The main visitors of the Tong-Ch'on amusement park are students in their twenties and thirties, and people whose incomes are below 300,000 Won a month. The purpose of visit is for a rest rather than for amusement and user prefer summer, while the user is so rare in wintertime. Those phenomena observed are somewhat different from the real purpose of a amusement park which is on purpose to make profits by offering entertainments to the users. So planner should pay attention to the three points. They are varieties, seasonable diversification and fantastic character of facilties, in the amusement park. 2. The access time of the Tong-Ch'on amusement park was 41 minutes, the use frequency was 4 times a year and resident time was 164 minutes. The relationship of the three factors are as follows; log Y(F) =1.7832-0.0277(A.T) R$^2$=0.75 Y(R. F)=31.8885+3.3217(A.T) R$^2$=0.53 Y(R. T)=224.8959-87.8309 1og(F) R$^2$=0.38 F;Use frequency(time/year) A.T;Access Time(minute) R.T;Resident Time(minute) 3. In the choice of space, there were much differences according to tole user's age, job, degree of education, companion type and purpose of use. 4. There are considerable correlation between use fluctuation and some factors. The factors are season(summer, winter) as a time, temperature, cloud amount, duration of sunshine, weather(rainy-day) as a climate and a day of the week(weekday, holiday) as a social system. The important variables are temperature, cloud amount, duration of sunshine and a day of the week(weekday, holiday) to estimate the user of amusementpark. 5. 1 can reduce the following two types of regression models. 1) log$\sub$e/ Y1 = 6.9114 + 0.l135 TEM + 0.00002 SUN -0.4068W1 + 0.4316 W3 (R$^2$= 0.94) 2) log$\sub$e/ Y2 = 7.2069 + 0.l177 TEM - 0.0990 CLO + 0.4880 W3 (R$^2$=0.95) Y; Number of User TEM; Temperature CLO; Amount of cloud SUN; Duration of Sunshine W1; Weekday W3; Holiday Those model is in order to estimate the user for management of Tong-Ch'on amusement park and use on the computation of facility sloe for reconstruction. Besides the amusement park, city park and outdoor recreation area could estimate of user through this method. But, I am not sure about the regression models because I did not apply the regression models to the other amusement park, city Park or outdoor recreation area. Therefore, I think that this problem needs to be studied on in the future.

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