• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1876-1884
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• 2020

Ethanol often accumulates during the process of wine fermentation, and mitophagy has critical role in ethanol output. However, the relationship between mitophagy and ethanol stress is still unclear. In this study, the expression of ATG11 and ATG32 genes exposed to ethanol stress was accessed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The result indicated that ethanol stress induced expression of the ATG11 and ATG32 genes. The colony sizes and the alcohol yield of atg11 and atg32 were also smaller and lower than those of wild type strain under ethanol whereas the mortality of mutants is higher. Furthermore, compared with wild type, the membrane integrity and the mitochondrial membrane potential of atg11 and atg32 exhibited greater damage following ethanol stress. In addition, a greater proportion of mutant cells were arrested at the G1/G0 cell cycle. There was more aggregation of peroxide hydrogen (H2O2) and superoxide anion (O2•-) in mutants. These changes in H2O2 and O2•- in yeasts were altered by reductants or inhibitors of scavenging enzyme by means of regulating the expression of ATG11 and ATG32 genes. Inhibitors of the mitochondrial electron transport chain (mtETC) also increased production of H2O2 and O2•- by enhancing expression of the ATG11 and ATG32 genes. Further results showed that activator or inhibitor of autophagy also activated or inhibited mitophagy by altering production of H2O2 and O2•. Therefore, ethanol stress induces mitophagy which improves yeast the tolerance to ethanol and the level of mitophagy during ethanol stress is regulated by ROS derived from mtETC.

• 한국IT서비스학회지
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• 제21권2호
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• pp.97-107
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• 2022

Recently, interest in smart factories is increasing. Investments to improve intelligence/automation are also being made continuously in manufacturing plants. Facility automation based on sensor data collection is now essential. In addition, we are operating our factories based on data generated in all areas of production, including production management, facility operation, and quality management, and an integrated standard information system. When producing LCD polarizer products, it is most important to link trace information between data generated by individual production processes. All systems involved in production must ensure that there is no data loss and data integrity is ensured. The large-capacity data collected from individual systems is composed of key values linked to each other. A real-time quality analysis processing system based on connected integrated system data is required. In this study, large-capacity data collection, storage, integration and loss prevention methods were presented for optimization of LCD polarizer production. The identification Risk model of inspection products can be added, and the applicable product model is designed to be continuously expanded. A quality inspection and analysis system that maximizes the yield rate was designed by using the final inspection image of the product using big data technology. In the case of products that are predefined as analysable products, it is designed to be verified with the big data knn analysis model, and individual analysis results are continuously applied to the actual production site to operate in a virtuous cycle structure. Production Optimization was performed by applying it to the currently produced LCD polarizer production line.

• Clinical and Experimental Reproductive Medicine
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• 제47권4호
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• pp.269-276
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• 2020

Objective: We investigated the impact of tyrosine kinase inhibitor (imatinib or dasatinib) coadministration with cyclophosphamide (Cp) on preantral follicle development in an in vitro mouse model. Methods: Seventy-three female BDF1 mice were allocated into four experimental groups: group A, saline; group B, Cp (25 mg/kg); group C, Cp (25 mg/kg) and imatinib (7.5 mg/kg); and group D, Cp (25 mg/kg) and dasatinib (7.5 mg/kg). Preantral follicles were isolated and cultured in vitro up to 12 days. Final oocyte acquisition and spindle integrity of metaphase II (MII) oocytes were assessed. Levels of 17β-estradiol and anti-Müllerian hormone (AMH) in the final spent media were measured by enzyme-linked immunosorbent assays, and the mRNA levels of Star, Sod1, Mapk3, and Casp3 in the final follicular cells were quantified by real-time polymerase chain reaction. Results: The percentage of MII oocytes per initiated follicle, the proportion of MII oocytes with normal spindles, and the 17β-estradiol level were similar in all four groups. The median AMH level in group B (7.74 ng/mL) was significantly lower than that in group A (10.84 ng/mL). However, the median AMH levels in group C (9.96 ng/mL) and group D (9.71 ng/mL) were similar to that in group A. The mRNA expression levels of Star, Sod1, Mapk3, and Casp3 were similar in all four groups. Conclusion: Coadministration of imatinib or dasatinib with Cp could preserve AMH production capacity in this in vitro mice preantral follicle culture model, and it did not affect MII oocyte acquisition.

• 한국건축시공학회지
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• 제23권3호
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• pp.315-326
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• 2023

The design principles and implementation of rebar lap splice in architectural structures are governed by building regulations. Nevertheless, the minimization of rebar-cutting waste (RCW) is often impeded by the mandatory requirements pertaining to the rebar lapping zone as prescribed in design codes. In real-world construction scenarios, compliance with these rules often falls short due to hurdles concerning productivity, quality, safety, time, and cost. This discrepancy between code stipulations and on-the-ground construction practices necessitates an academic exploration. The goal of this research was to delve into the effect of rebar lap splice placement on the robustness and constructability of building edifices. The study initially took on a review of the computation of rebar lapping length and the rules revolving around the lapping zone. Following this, a structural robustness and constructability examination was undertaken, focusing on adherence to the lap splice zone. The interpretations and deductions of the research led to the following insights: (1) the efficacy of rebar lap splice is not solely contingent on the moment, and (2) the implementation of rebar lap splice beyond the specified zone can match the structural integrity and robustness of those confined within the designated area. As a result, the constraints on the rebar lapping zone ought to be revisited and possibly relaxed. The conclusions drawn from this research are anticipated to reconcile the disconnect between building codes and practical construction conditions, furnishing invaluable academic substantiation to further the endeavor of achieving near-zero RCW.

• Composites Research
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• 제22권6호
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• pp.45-51
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• 2009

치아의 와동에 수복된 치과용 복합레진을 광(LED) 조사를 통해 경화 수축시키면서 음향방출(acoustic emission, AE)법을 이용하여 와동 벽면과 복합레진 사이의 계면상태를 실시간 해석하였다. 복합레진의 중합 수축에 의해 AE 신호들이 검출되었는데 계면 접착 상태가 나쁜 경우, 경화 수축률이 큰 초기 1분 동안 많은 AE 사상들이 관측되었으며, 이들 AE는 주파수범위가 주로 100-200kHz인 돌발형이었다. 인간치아 재질의 시편에서 발생된 AE 사상수는 스테인리스 스틸 시편보다 적었으나, PMMA 시편보다는 많았고 접착제의 도포 상태가 양호할수록 검출되는 AE 사상수는 적었다. AE 파라미터의 특성은 복합레진과 링재 사이의 접착부에서 발생하는 인장형 균열전파 및 복합레진의 수축에 따른 링재의 압축거동과 관련되었으며, 음향방출법이 치아 수복물의 접착성에 대한 비파괴 검사법으로써 유효함을 알았다.

• 한국컴퓨터정보학회논문지
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• 제14권4호
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• pp.101-109
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• 2009

오라클 DBMS의 8i버전에서는 암호화 모듈이 기본적으로 장착되어 있으나, 암호화 모듈은 성능저하가 야기되어 제한적으로 적용되고 있다. 본 논문에서는 인덱스검색, 객체관리 혼란, 암호화로 인한 심각한 DB성능 저하, 실시간 데이터 암호화 미지원 IP기반의 데이터 접근제어 미지원으로 인한 기술별로 DB 보안의 문제점을 분석한다. 그리고 DB 보안의 가용성을 향상시키기 위해 암호화기술의 대체수단인 DB Masking 기법을 활용한 종합적인 보안 프레임워크를 제안한다. 취약점 개선안으로 가상계정을 이용하여 보안등급별로 DB Masking 기준을 설정하고, 가상계정을 통한 사용자 인증과 SQL문의 사전, 사후 결재 및 무결성을 체크하고, 감사 로그로 수집하여 DB를 안전하게 관리 할 수 있는 방안으로 활용한다.

• Animal Bioscience
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• 제36권9호
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• pp.1403-1413
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• 2023

Objective: Intestinal alkaline phosphatase (IAP) maintains intestinal homeostasis by detoxifying bacterial endotoxins and regulating gut microbiota, and lipid absorption. Antibiotics administered to animals can cause gut dysbiosis and barrier disruption affecting animal health. Therefore, the present study sought to investigate the role of IAP in the intestinal environment in dysbiosis. Methods: Young male mice aged 9 weeks were administered a high dose of antibiotics to induce dysbiosis. They were then sacrificed after 4 weeks to collect the serum and intestinal organs. The IAP activity in the ileum and the level of cytokines in the serum samples were measured. Quantitative real-time polymerase chain reaction analysis of RNA from the intestinal samples was performed using primers for tight junction proteins (TJPs) and proinflammatory cytokines. The relative intensity of IAP and toll-like receptor 4 (TLR4) in intestinal samples was evaluated by western blotting. Results: The IAP activity was significantly lower in the ileum samples of the dysbiosis-induced group compared to the control. The interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha concentrations were significantly higher in the ileum samples of the dysbiosis-induced group. The RNA expression levels of TJP2, claudin-3, and claudin-11 showed significantly lower values in the intestinal samples from the dysbiosis-induced mice. Results from western blotting revealed that the intensity of IAP expression was significantly lower in the ileum samples of the dysbiosis-induced group, while the intensity of TLR4 expression was significantly higher compared to that of the control group without dysbiosis. Conclusion: The IAP activity and relative mRNA expression of the TJPs decreased, while the levels of proinflammatory cytokines increased, which can affect intestinal integrity and the function of the intestinal epithelial cells. This suggests that IAP is involved in mediating the intestinal environment in dysbiosis induced by antibiotics and is an enzyme that can potentially be used to maintain the intestinal environment in animal health care.

• Animal Bioscience
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• 제36권12호
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• pp.1796-1805
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• 2023

Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.

• 한국컴퓨터정보학회논문지
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• 제29권1호
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• pp.121-130
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• 2024

본 논문에서는 함정 전투체계 소프트웨어 설치 시 발생 가능한 데이터 이상을 확인하는 설치 방안을 제안한다. 최근 저궤도 위성 통신과 같은 무선 통신을 이용한 정보교환 방법이 대중화되며, 함정 전투체계에서도 무선망을 이용한 여러 활용 방안이 논의되고 있다. 활용 방안 중 하나로서 무선망 통신을 이용한 설치를 함정 전투체계에 적용함으로써 실시간으로 전투체계 성능을 향상시킬 수 있는 방법이 가능해질 것으로 기대한다. 하지만 무선망의 경우 유선망보다 상대적으로 보안상 취약하므로 더 많은 보안 대책이 강구된다. 본 논문에서는 암호화 방식을 통해 다수의 노드에 파일을 전송하고 파일 설치 이후 유효성 검사를 수행함으로써, 전송 도중 위/변조 여부를 판단하여 정상적으로 설치됨을 확인한다. 제안한 방법의 함정 전투체계 적용 가능성을 보이기 위하여 전송 성능 및 보안성, 안정성 등을 평가하였으며, 이를 바탕으로 함정 전투체계에 적용하기 충분한 수준의 결과물을 도출하였다.

• 한국항해항만학회지
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• 제33권10호
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• pp.691-697
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• 2009

현재 운영되고 있는 해양용 DGPS 시스템은 하드웨어 RSIM 방식으로서 DGPS 구조개선시 고려해야 할 위성항법시스템의 다원화와 미래 해양 사용자의 최소 요구성능을 만족시킬 수 있는 기술 적용에 효율적이지 못하다는 문제가 있다. 본 논문에서는 이러한 문제를 해결하기 위하여 소프트웨어 RSIM 방식으로 해양용 DGPS 시스템을 구조개선하는 방안을 연구한다. 이를 위해 본 논문은 해양용 DGPS 시스템에 적합한 소프트웨어 RSIM 방식을 제안하고, 제안한 방법의 기술적 타당성 분석을 위하여 광대역폭, 높은 표본화 주파수, 데이터 고속전송 사양을 갖는 RF 모듈과 DSP 모듈을 제작하며, 이를 이용하여 제안한 방법을 적용한 해양용 DGPS 시스템을 구현한다. 그리고 신호처리 소요시간 분석을 통하여 개인용 컴퓨터 환경에서 제안한 소프트웨어 RSIM 방식이 실시간으로 운영됨에 문제가 없음을 보인다. 마지막으로 본 논문은 제안한 방법으로 설계된 DGPS 시스템의 보정정보 정확도가 RTCM 221-2006-SC104-STD을 통해 규정된 국제기구 요구성능을 만족시키고 있음을 확인한다.