• Journal of Microbiology and Biotechnology
• /
• v.29 no.10
• /
• pp.1543-1552
• /
• 2019

Salmonella is a common zoonotic and foodborne pathogen that causes high morbidity and mortality in developing countries. In this study, we established and validated a polymerase spiral reaction (PSR) assay which targeted the conserved invasion gene (invA) of Salmonella by SYBR Green I indicator methods. Subsequently, assays for determination of the optimal conditions for optimal specificity and sensitivity of PSR were performed. We performed comprehensive evaluations using loop-mediated isothermal amplification (LAMP) and real-time PCR. A total number of 532 samples of daily food were analyzed by PSR. Twenty-seven bacterial strains were tested in the specificity assay, from which positive results were obtained only for 14-Salmonella strains. However, none of the 13 non-Salmonella strains was amplified. Similarly with LAMP and real-time PCR, the detection limit of the PSR assay was 50 CFU/ml. The PSR method was also successfully applied to evaluate the contamination with Salmonella in 532 samples of daily food, corroborating traditional culture method data. The novel PSR method is simple, sensitive, and rapid and provides new insights into the prevention and detection of foodborne diseases.

• The Korean Journal of Malacology
• /
• v.27 no.4
• /
• pp.387-393
• /
• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.

• Nutrition Research and Practice
• /
• v.8 no.2
• /
• pp.205-212
• /
• 2014

BACKGROUND/OBJECTIVES: Dietary behavior and life stress in adolescence is related to growth rate and learning ability. This study was conducted to identify the relations between dietary habits, dietary attitude nutritional knowledge and life stress among high school girls in Korea and China. SUBJECTS/METHODS: The subjects of this study were 221 high school girls in Korea and 227 high school girls in China. The questionnaire were about dietary habits, dietary attitude, nutritional knowledge and life stress. RESULTS: The dietary habits of chinese girls were healthier than those of Korean girls with a significant difference (P < .001). There was no significant difference in dietary attitude between Korean girls and Chinese girls. Korean girls had more nutritional knowledge than Chinese girls with a significant difference (P < .001). Korean girls did less physical exercise but spent more time watching TV and using PCs, compared to Chinese girls. Korean girls' degree of confidence in nutrition information that they had learned and their performance in their real lives were low. Also, they had a low level of awareness of the need for nutritional education. There was no significant difference in life stress between the two groups. Dietary habits had a significantly negative correlation with life stress in both Korean and Chinese girls (P < .01, P < .001). As for Chinese students, dietary attitude had a negative correlation with life stress with a significant difference (P < .05). As for Korean girls, nutritional knowledge had a negative correlation with life stress with a significant difference (P < .05), which means as life stress was less, dietary habits were better. CONCLUSIONS: This study shows that effective nutrition education programs should include components that encourage application of learned nutrition information to real life, increase physical exercise and reduce life stress.

• Journal of Families and Better Life
• /
• v.14 no.2
• /
• pp.157-170
• /
• 1996

Even if holding debt may be a rational means for household to maximize utility under any circumstance and any time through the family life cycle most households have some difficulty to determin and keep the moderate debt amount. The purpose of this study is to identify the characteristics of household's debt and the factor associated with debt. Data used in this study consisted of 4,009 households. The results of this study were as follows; Among 4,009 households 1,400 housholds?(34.9%) owed. Age education and occupation of household header liquid and real asset and housing ownership had significant effects on whether household having debt. The real and liquid asset had significantly positive relation with the liabilities of financial agency whereas the occupation of household header liquid asset and unearned income with private liabilities. Finally age education and occupation of household header home ownership and liquid asset had significantly negative effect on the total a ount of debt while earned and unearned income and real asset had positive one.

• International conference on construction engineering and project management
• /
• 2007.03a
• /
• pp.729-738
• /
• 2007

The Public Private Partnership/Private Finance Initiative (PPP/PFI) schemes have made the private sector become a major participant involved in the development of infrastructure systems along with the government. Due to more integrated efforts among project participants and longer concession period, PPP/PFI projects are inherently more complex and risky. It is therefore very important to proactively manage the risks involved throughout the project life cycle. Conventional risk management strategies sometimes ignore managerial flexibility in the planning and execution process. This paper starts with a revised risk management framework which incorporates the real option concept. Following the presentation of the framework, a new risk classification is proposed which leads to different ways of structuring options in a project according to the stage of the project life cycle. Finally, the paper closes by discussing other issues concerning option modeling and negotiation.

• Food Science of Animal Resources
• /
• v.31 no.6
• /
• pp.851-857
• /
• 2011

This study was conducted to develop a real time reverse transcriptase-PCR (RT-PCR) method for the detection of viable Enterobacteriaceae in milk using primers based on the genes of ribosomal proteins S11 and S13 and to determine effects of heating and subsequent treatments on the threshold cycle (Ct) of the real time RT-PCR. Total RNA was isolated from 17 strains of bacteria including 11 strains of Enterobacteriaceae suspended in milk using a modified Tri reagent method. SYBR Green Master Mix was added to the RNA and the mixture was subjected to the real time RT-PCR. The Cts of eleven type strains of the Enterobacteriaceae in milk ($10^7$ cells) in the real time RT-PCR ranged from 21.5 to 24.6. However, the Cts of Pseudomonas fluorescens, Acinetobacter calcoaceticus, and three gram-positive bacteria were more than 40. The real time RT-PCR detected as low as $10^3$ cells in agarose gel electrophoresis. The Cts increased from 22.0 to 34.2 when milk samples contaminated with Escherichia coli ($10^7$ cells/mL) were heated at $65^{\circ}C$ for 30 min. In addition, subsequent incubation at $37^{\circ}C$ for 6 and 24 h increased the Cts further up to 36.2 and 37.2, respectively. Addition of RNase A to the bacterial suspension obtained from the heated milk and subsequent incubation at $37^{\circ}C$ for 1 h increased the Cts to more than 40. The results of this study suggests that pretreatment of bacterial cells heated in milk with RNase A before RNA extraction might enhance the ability to differentiate between viable and dead bacteria using real time RT-PCR.

• Journal of Korean Society of Rural Planning
• /
• v.20 no.2
• /
• pp.1-10
• /
• 2014

Smartphones change the picture of data and information sharing and make it possible to share various real-time flooding data and information. The vulnerability indicators of farmland inundation is needed to calculate the risk of farmland flood based on changeable hydro-meteorological data over time with morphologic characteristics of flood-damaged areas. To find related variables show the vulnerability of farmland inundation using the binary-logit model and correlation analysis and to provide vulnerability indicators were estimated by fuzzy set method. The outputs of vulnerability indicators were compared with the results of Monte Carlo simulation (MCS) for verification. From the result vulnerability indicators are applicable to mobile_based information system of farmland inundation.

• Korean Journal of Human Ecology
• /
• v.14 no.5
• /
• pp.859-869
• /
• 2005

The purpose of this study was to research university students' awareness of gender equality and practice of clothing and textiles education contents(classifying them into four units; choice and dressing of clothes, textile and management of clothes, design and sewing of clothes, and knitting and embroidery) in their real life. This is the investigation of 344 university students living in Choongchungnam bukdo, Junrabukdo, and Daejeon. In this statistical analysis, SPSS 10.0 for Windows Program was utilized to calculate percentage, mean and standard deviation. Also, these data were verified by t-test, One-way Anova, Duncan's multiple range, Pearson's correlation coefficient. The results of this research were as follows: The more university students have awareness of gender equality, the more they have practice of clothing and textiles education content in their real life. There was a significant difference in practical awareness of contents of clothing and textiles education according to demographic variables and correlation of variables differed significantly. As a conclusion, university students' awareness of gender equality plays an important role in practice of clothing and textiles education contents in their real life. These results would provide fundamental information for improving students' critical practice.

• Journal of Korean Home Economics Education Association
• /
• v.15 no.1
• /
• pp.57-68
• /
• 2003

The purpose of this study was to research high school students’ practical awareness about the contents(classify them into four units. choice and dressing of clothes. textile and management of clothes. design and sewing of clothes. knitting and embroidery) clothing and textiles education in their real life. This is the investigation of which 576 high school students located in Choongchungnam$.$bukdo. Junrabukdo, Daeseon. In this statistical analysis. SPSS 10.0 for Windows Program was utilized to calculate percentage. mean and standard deviation. Also. these materials were verified by t-test. One-way Anova . Pearson’s correlation coefficient. The main results of obtained this study are as follows High school students’ practical awareness about the contents of clothing and textiles education in their real life appears to be low(only 9 items were over median point of 3.0). There was a significant difference in Practical awareness of contents of clothing and textiles education according to demographic variables. and correlation of variables differed significantly. These results should be provide fundamental information for improving students’ practical practice in their real life Since this study has touched only limited research on the topic of clothing and textiles education. it is essential that further research covering a wider array of themes be done in the future.

• Journal of The Korean Association of Information Education
• /
• v.14 no.4
• /
• pp.555-560
• /
• 2010

The purpose of this study is to investigate the effect of learning algorithm which uses real-life examples including the concept of algorithm on the logical thinking of elementary school students. For this purpose, the experiment was performed by pre-GALT test, a case selection of algorithm which can be taught in real-life, experiment treatment after completing teaching plan, post-GALT test, and paired sample t-test on the results of pre and post GALT in order. As a result, changes in the degree of logical thinking ability and in five sub-regions(conservative logic, proportional logic, combinatorial logic, probabilistic logic, controlling variables) composing of logical thinking obtained statistically significant results in .05 significance level but changes in the correlational logic couldn't obtain the significant results.