• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.495-510
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• 2022

In this study, fluorescence hyperspectral imaging (FHSI) was used for the rapid, non-destructive detection of fake, manmade eggs from real eggs. To identify fake eggs, protoporphyrin IX (PpIX)-a natural pigment present in real eggshells-was utilized as the main indicator due to its strong fluorescence emission effect. The fluorescence images of real and fake eggs were acquired using a line-scan-based FHSI system, and their fluorescence features were analyzed based on spectroscopic techniques. To improve the detection performance and accuracy, an optimal waveband combination was investigated with analysis of variance (ANOVA), and its fluorescence ratio images (588/645 nm) were created for visualization of the real eggs between two different egg groups. In addition, real and fake eggs were scanned using a one-waveband (645 nm) handheld fluorescence imager that can perform real-time scanning for on-site applications. Then, the results of the two methods were compared with one another. The outcome clearly shows that the newly developed FHSI system and the fluorescence handheld imager were both able to distinguish real eggs from fake eggs. Consequently, FHSI showed a better performance (clearer images) compared to the fluorescence handheld imager, and the outcome provided valuable information about the feasibility of using FHSI imaging with ANOVA for the discrimination of real and fake eggs.

• Journal of Energy Engineering
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• v.8 no.4
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• pp.605-611
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• 1999

Conventional methods for assessing remaining life of critical high temperature components in fossil power plants rely on nondestructive inspection practices and accompanying life analysis based on fracture mechanics By using these conventional methods. It has been difficult to perform uninterrupted in-service inspection for life prediction. Thus, efforts have been made for developing on-line remaining life monitoring systems employing information on the shape of structures, operating variables and material properties. In thus study, a software for on-line life monitoring system which performs real-time life evaluation of a high temperature system headers was developed. The software is capable of evaluating creep and fatigue life usage from the real-time stress data calculated by using temperatures/stress transfer Green functions derived in advance for the specific headers. The major benefits of the developed software life in determining future operating schedule, inspection interval, and replacement plan by monitoring real-time life usage based on prior operating history.

• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1301-1306
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• 2012

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 non-respiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ${\leq}35$ and the ratio of real-time RT-PCR and real-time PCR load was ${\geq}1.51$. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including non-respiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

• Journal of fish pathology
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• v.34 no.2
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• pp.161-168
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• 2021

Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine, spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 10⁸ copies/mg tissue) of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 10³~4.6 × 10⁵ copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata, it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 × 10³ copies/mg tissue) and body kidney (9.1 × 10³ copies/mg tissue). In order to investigate whether myxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei and P. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNA copy number of E. leei from rearing water of farm-A and farm-B were 8 × 10⁴ and 5 × 10⁵ copies/L, respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A, farm-B and farm-C showed 0, 2.0 × 10⁶ and 5.1 × 10⁶ copies/L, respectively.

• Journal of Korea Multimedia Society
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• v.10 no.12
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• pp.1714-1725
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• 2007

This paper describes a top-down behavior planning framework in a simulation game from personality to real life action selection. The combined behavior creating system is formed by five levels of specification, which are personality definition, motivation extraction, emotion generation, decision making and action execution. Along with the data flowing process in our designed framework, NPC selects actions autonomously to adapt to the dynamic environment information resulting from active agents and human players. Furthermore, we illuminate applying Gaussian probabilistic distribution to realize character's behavior changeability like human performance. To elucidate the mechanism of the framework, we situated it in a restaurant simulation game.

• School Mathematics
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• v.3 no.2
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• pp.233-248
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• 2001

In pthis aper, mathematical terms in 7th elementary mathematics curriculum(from now, in short, 7th curriculum)are reexamined critically. In 7th curriculum there are 123 terms, which seems to be selected cautiously But it is not sure. There are lots of evidences for selecting terms incautiously, Through these evidences, following conclusions are induced: (1) Terms were not selected strictly. There are many terms omitted in 7th curriculum, which are necessary for understanding mathematical concepts. (2) There were no rational principles for selecting terms in 7th curriculum. Any rational principles can not be found out among terms in 7th curriculum. (3) Mathematical terms and real life terms in 7th curriculum were not distinguished explicitly. There were some real life terms in 7th curriculum, which were significant for understanding mathematical concepts. But other real life terms which is significant also for understanding mathematical concepts were not contained in 7th curriculum.

• Journal of Information Technology Applications and Management
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• v.21 no.3
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• pp.97-117
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• 2014

People can differently act online than in real-life. Their virtual identities may allow them to express themselves more freely. In this perspective, there is a self-discrepancy between virtual- and real-life. Due to the anonymity, people can feel free to express who actually they are online without considering social pressure. Especially, for those people who live in a repressed society, virtual-selves can play a significant role to achieve their well-being. For this reason, a self-discrepancy between actual- and virtual-self related to well-being was studied. With 183 survey responses, political freedom, extraversion and neuroticism are identified as significant influencers of subjective well-being (SWB). In addition, an increase in self-discrepancy between virtual- and real-life for 'unhappy' people leads to an increased online well-being, which is contradictory to Higgins' self-discrepancy theory.

• Asia Marketing Journal
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• v.21 no.4
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• pp.45-57
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• 2020

With the development of new technologies, virtual reality (VR) is emerging as a new consumer marketing platform. Yet, only a few studies have investigated VR in the consumption context. The current research explores how experiencing a product through VR influences consumer attitude and purchase intention for the product. Although assessing products in real life is believed to be more diagnostic than that through VR, the present research revealed that experiencing a product through VR could lead to a more positive attitude toward and higher purchase intention for the product. We also demonstrated that "enjoyment" has a mediating effect while evaluating the product through VR. To compare product experience through VR to that in real life, we employed the HTC Vive VR system to implement a VR environment. In the VR condition, participants evaluated plastic building blocks using a VR headset and a motion controller; and in the real life (reality) condition, they evaluted plastic building blocks printed using a 3D printer to make them look exactly the same as the blocks used in the VR condition. Finally, implications and suggestions are discussed.

• Asia Marketing Journal
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• v.25 no.2
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• pp.61-70
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• 2023

Although research on Virtual Reality (VR) has uncovered numerous technical advantages of VR over traditional media, little is known about how individual VR users with varying prior experience respond to VR differently. This paper examines the effect of users' prior VR experience on their subsequent real-life behavior in the domain of charitable consumption. Specifically, we find that compared to experienced VR users, novice VR users are more likely to support a charitable cause in real life (e.g., ocean conservation) after experiencing this cause in VR. The increased support among novice VR users occurs because they perceive the use of VR to be more novel. We find a boundary of this effect such that when VR is used to promote a noncharitable cause, novice VR users no longer increase their real-life support after VR. This research offers new possibilities for future studies on the use of VR in societal marketing.

• Korean Journal of Microbiology
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• v.43 no.2
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• pp.91-99
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• 2007

For the detection of Human Immunodeficiency Virus (HIV), multiple and ultra-rapid real-time PCR methods were developed. The target DNA sequences were deduced from HIV-1 specific 495bp partial env gene (gi_1184090) and from HIV-2 specific 294 bp partial env gene (gi_1332355), and were synthesized by using PCR-based gene synthesis on the reason of safety. Ultra-rapid real-time PCR was performed by $Genspector^{TM}$ using microchip-based, $1\;{\mu}l$ of reaction volume with extremely short time in each 3 step in PCR. The detection including DNA-amplification and melting temperature analysis was completed inner 15 minutes. The HIV-1 specific 117 bp-long and HIV-2 specific 119 bp-long PCR products were successfully amplified from minimum of template,2.3 molecules of each env gene. This kind of real-time PCR was designated as ultra-rapid real-time PCR in this study and it could be applied not only an alternative detection method against HIV, but also other pathogens using PCR-based detection.