• 연구논문집
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• s.27
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• pp.47-55
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• 1997

Fatigue life prediction of mechanical components is necessary to develop new products, which is very expensive and time-consuming. This paper reviews technologies proposed for computation of dynamic stress in mechanical components. The methods based on multibody dynamics are considering more real operational conditions than other methods. The technology for fatigue life prediction without the prototype for experiment results in cost and time saving. This technology can be applied to design of various mechanical components like carbody.

• Journal of Family Resource Management and Policy Review
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• v.3 no.2
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• pp.91-103
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• 1999

This paper gropes how to connect philosophy of household management(PHM) to real life. PHM is to think and to act for better quality of life, so PHM should contain not only theory but also practice in itself. In that sense, PHM is consist of two parts: one is viewpoints how to deal with home management and the other is practice. This paper presents 5 main viewpoints of PHM. Then, it explains PHM can connect with education of human nature, and ecological value in PHM can connect with practice of life. Also, it proposes a guide for practice.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.100-108
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• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• Food Science and Biotechnology
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• v.18 no.5
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• pp.1118-1123
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• 2009

Event-specific real-time polymerase chain reaction (PCR) detection method for genetically modified (GM) maize MIR604 was developed based on integration junction sequences between the host plant genome and the integrated transgene. In this study, 2 primer pairs and probes were designed for specific amplification of 100 and 111 bp DNA fragments from the zSSIIb gene (the maize endogenous reference gene) and MIR604. The quantitative method was validated using 3 certified reference materials (CRMs) with levels of 0.1, 1, and 10% MIR604. The method was also assayed with 14 different plants and other GM maize. No amplification signal was observed in real-time PCR assays with any of the species tested other than MIR604 maize. As a result, the bias from the true value and the relative deviation for MIR604 was within the range from 0 to 9%. Precision, expressed as relative standard deviation (RSD), varied from 2.7 to 10% for MIR604. Limits of detections (LODs) of qualitative and quantitative methods were all 0.1%. These results indicated that the event-specific quantitative PCR detection system for MIR604 is accurate and useful.

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.51-57
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• 2017

Digital signage is becoming part of daily life across a wide range of visual advertisements segments market used in stations, hotels, retail stores, hotels, etc. The current digital signage system used in market is generally works on limited user interactivity with static contents. In this paper, a new approach is proposed using computer vision based dynamic audience adaptive cost-effective digital signage system. The proposed design uses the Camera attached Raspberry Pi Open source platform to employ the real-time audience interaction using computer vision algorithms to extract facial features of the audience. The real-time facial features are extracted using Haar Cascade algorithm which are used for audience gender specific rendering of dynamic digital signage content. The audience facial characterization using Haar Cascade is evaluated on the FERET database with 95% accuracy for gender classification. The proposed system, developed and evaluated with male and female audiences in real-life environments camera embedded raspberry pi with good level of accuracy.

• International conference on construction engineering and project management
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• 2013.01a
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• pp.386-389
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• 2013

Under climate change and urbanization, rainwater harvesting (RWH) systems are emerging as an alternative source of water supply because of growing concern about water sustainability. RWH systems can satisfy the various watering needs and provide the environmental benefits of lessening the damages from flood, drought, and runoff. The economic success of a RWH system is vitally concerned with the determination of the design capacity of storage tank to be built in the system. The design capacity is determined by the factors of average annual rainfall, period of water scarcity, and water price during the whole life-cycles. Despite the high uncertainties inherent in these factors, the current engineering design of RWH system construction often assumes that storage tanks should be built all at once. This assumption implicitly ignores the managerial flexibility in responds to the future as new information comes out-the right to build storage tanks stage by stage depending on the evolution of demand. This study evaluates the value of a multistage storage tank construction using a real option approach. A case study involving a typical RWH system construction in Jeonju, the Republic of Korea is conducted. The managerial flexibility obtained from the real option perspective allows engineers to develop investment strategies to better cope with the issue of water sustainability.

• Mycobiology
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• v.49 no.1
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• pp.86-88
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• 2021

The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.09a
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• pp.446-447
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• 2005

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2013.11a
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• pp.79-85
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• 2013

• Korean Journal of Adult Nursing
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• v.14 no.3
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• pp.438-448
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• 2002

Purpose: This study is aimed to search the lived experiences of the participants and to analyze the contents so that we can be assured about what the elderly wish and find the appropriate nursing intervention for them in real life. Method: This is based on a phenomenological approach. Participants of this study consist of people older than 65 years old. Data was collected from May to August in 2001. Data collection was done through in-depth interviews and observations. The time used in each interview was from 50 minutes to 2 hours. Each participant was interviewed three to five times. Giorgi's phenomenological analysis was used in data analysis. Result: The elder's wishes are 1) a respected life, 2) a happy life, 3) a peaceful life, 4) a independent life, 5) a financially independent life, 6) a satisfactory life, 7) a mind to depend on someone, 8) a serving life, 9) a historic family, 10) a happy-ending life. Conclusion: The study will contribute for the nursing intervention to enhance the quality of life and successful aging of the elderly.