• Title/Summary/Keyword: Reaction media

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Detection of Cellulolytic Activity in Ophiostoma and Leptographium species by Chromogenic Reaction

  • Hyun, Min-Woo;Yoon, Ji-Hwan;Park, Wook-Ha;Kim, Seong-Hwan
    • Mycobiology
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    • v.34 no.2
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    • pp.108-110
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    • 2006
  • To understand the ability of producing cellulolytic enzyme activity in the sapstaining fungi, four species of Ophiostoma and two species of Leptographium were investigated in the culture media containing each of cellulose substrates such as CM-cellulose, Avicel and D-cellobiose and each of chromogenic dyes such as Congo-Red, Phenol Red, Remazol Brilliant Blue and Tryphan Blue. When the fungi were grown for $5{\sim}7$ days at $25^{\circ}C$, the formation of clear zone by chromogenic reaction around the margin of the fungal colony was demonstrated in all the culture media Congo-Red containing CM-cellulose. There was difference in the formation of clear zone among the dyes. Only Ophiostoma setosum and Leptographium spp. showed cellulolytic activity to the three substrates. Overall, the results of this study show that ophiostomatoid sapstaining fungi can produce cellulolytic enzymes.

Story Structure & Media Traits of Japanese Comic Book -focusing on Shinyashokudo- (일본만화의 이야기구조와 미디어적 속성 -<심야식당>을 중심으로-)

  • Hong, Kyung-Soo
    • The Journal of the Korea Contents Association
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    • v.10 no.11
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    • pp.162-168
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    • 2010
  • Comic book has a different story structure from western movies, i.e. New Character->reaction->food->master mediation->effect of food->disappearance ->conclusion. Comic book is full of media traits. The master is like a shaman, who console people with food of memory. Food also is a media, which carries message of memory instead of savor itself. Restaurant is a media, in which many events occur and rhetoric of rumor pervade. Foods of memory massage our feeling sense, like a media. In conclusion, comic book has a analogous role to that of Marshal Mcluhan's Media theory.

Phosphorus Removal from Wastewater by CaCO3 Media (탄산칼슘 담체를 이용한 폐수내의 인 제거)

  • Kim, Moon Ki;Park, Jae Hong;Lee, Kwang Hyun;Joo, Hyun Jong
    • Journal of Korean Society on Water Environment
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    • v.25 no.4
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    • pp.515-521
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    • 2009
  • In this study, the applicability of $CaCO_3$ as a seed material for crystallization reaction was tested. $CaCO_3$ was ground to lesser than 425 mesh and was made to media mixed with binder. Batch experiment was to investigate the ${PO_4}^{3-}-P$ removal efficiency of different parameters such as $CaCO_3$ dosage and binder ratio, size, type and mass of media. In addition, the effect of phosphorus removal from wastewater was tested using a lab-scaled crystallization reactor. At the results of the batch test, phosphorus removals were improved with increasing $CaCO_3$ dosage and media mass but were decreased with increasing media size. Moreover, phosphorus removals were influenced by specific surface area but media type. The average T-P and ${PO_4}^{3-}-P$ removal efficiency in a lab-scaled crystallization reactor with $CaCO_3$ media for wastewater were shown to be 60.2% and 60.3% for 18 days of operation time.

Performance Evaluation of a Bioreactor Partially Packed with Porous Media Containing a MA (Microorganism Activator) (미생물 활성물질이 내재된 담체를 이용한 생물반응조의 성능 평가)

  • Park, Jong-Hoon;Hong, Seok-Won;Choi, Yong-Su;Lee, Sang-Hyup;Kim, Seung-Jun;Kang, Seun-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.1
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    • pp.47-55
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    • 2007
  • The waste water treatment facility at rural and mountainous region in catchment areas of dams should be small scale. The wastewater treatment facility of small scale has some specification as follows;1)simple process, 2)low maintenance cost, and 3)high removal efficiency. So, we developed the bioreactor which can be satisfied with the specification of small scale waste water treatment facility. The bioreactor consisted of the anoxic and oxic zone. The two zones were effectively separated by cone type baffle. By the effective separation through CTB, the nitrification and denitrification reaction occurred effectively. Therefore, the removal efficiency of total nitorgen (TN) increased compared to other types of baffle. And, we put into the bio activated media in oxic zone to increase the concentration and activity of microbiology. The media contained the components which were made of many kinds of the minerals to increase the activity of microbiology. Additionally, we observed that the phosphate removal efficiency increased by bio activated media. This is resulted from the coagulation-sedimentation reaction by mineral in components. The average removal efficiencies of TN and TP during Run 2 were 69 and 89% which were 4 and 25% higher than those during Run 1 without the MA, respectively. For BOD, COD, SS and TKN, the average removal efficiencies at Run 2 were slightly higher than those at Run 1. Therefore, we could maintain the high concentration and high activity of microbiology through bioreactor developed in this study. And the removal efficiency of TN and TP increased.

The Precaution Duty and the Product Liability for Adverse Reactions to the Contrast Media (조영제 부작용에 대한 주의의무와 제조물책임)

  • Kang, Yeong-Han
    • Journal of radiological science and technology
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    • v.30 no.4
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    • pp.305-311
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    • 2007
  • Contrast medium is a useful drug for radiological examinations and usability of it gradually increases while it has some inevitable adverse reaction and it is difficult to predict the occurrence and the degree of adverse reactions. Although the patient consented permission for the use of contrast media, the hospital could not be exempted from the responsibility for the adverse contrast media reaction. During radiological contrast media examination, the radiologist and the contrast media producer have the duty of precaution, prediction and avoid adverse results. In addition, they have reliabi lity of patient remedy for neglecting the duty. Since contrast medium are manufactured or processed as movable properties, the manufacturers are bound to the product liability if damages occur due to the defects in pharmaceuticals. In consideration of the characteristics of product liability, it is necessary to demand high degree of duty of care and diligence from producer or to reduce patient's burden of proof in a product liability lawsuit. For securing compensation ability and liability implementation, products compensation liability insurance is required for contrast medium manufacturers. In conclusion, contrast medium producer has legal liability for adverse reactions and the contract concluded with producer and hospital including legal liability will reduce liability of hospital and radiologist, patient.

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Elastic Resistance Exercise for the Elderly on the Magnitude of Frequency and Variability of Ground Reaction Force Signals during Walking (고령자 보행 시 탄성저항운동이 지면반력 신호의 주파수 크기와 variability에 미치는 영향)

  • Seo, Se-Mi;Ryu, Ji-Seon
    • Korean Journal of Applied Biomechanics
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    • v.18 no.4
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    • pp.49-57
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    • 2008
  • The purpose of this study was to determine the effects of 12-week elastic resistance exercise for the elderly on the magnitude of frequency and variability of ground reaction force signals. To this aim, total 12 elderly women aged in their 70 were participated in this study and asked to do a 12-week elastic resistance exercise program. FFT(fast Fourier Transform) was used to analyze the frequency domain analysis of the ground reaction forces's signals and an accumulative PSD (power spectrum density) normalized by support phase of walking was calculated to reconstruct the certain signals. To estimate the gait stability between the before and after exercise, values of variability were determined in a coefficient of variance. The magnitude of frequency and variability analysis for media-lateral signal revealed significantly less after exercise (p<.05). In contrast, variability of this signal's frequency that have used to evaluate the local stability during walking exhibited significantly greater after exercise(p<.05). In summary, magnitude frequency and variability of media-lateral ground reaction force's signal were significantly changed after a 12-week elastic resistance exercise.

Limitations of the Transition State Variation Model. Part 8. Dual Reaction Channels for Solvolyses of 3,4-Dimethoxybenzenesulfonyl Chloride

  • Koo, In-Sun;Kwon, Eun-Ju;Choi, Ho-June;Yang, Ki-Yull;Park, Jong-Keun;Lee, Jong-Pal;Lee, Ikc-Hoon;Bentley, T. William
    • Bulletin of the Korean Chemical Society
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    • v.28 no.12
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    • pp.2377-2381
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    • 2007
  • Solvolyses of 3,4-dimethoxybenzenesulfonyl chloride (DSC) in water, D2O, CH3OD, and in aqueous binary mixtures of acetone, acetonitrile, 1,4-dioxane, ethanol, methanol, and 2,2,2-trifluoroethanol (TFE) have been investigated at 25.0 oC. Kinetic solvent isotope effects (KSIE) in water and in methanol and product selectivities in alcohol-water mixtures are also reported. The Grunwald-Winstein plot of first-order rate constants for the solvolyic reaction of DSC with YCl shows marked dispersions into separated lines for various aqueous mixtures. With use of the extended Grunwald-Winstein equation, the l and m values obtained are 1.12 and 0.58 respectively for the solvolyses of DSC. The relatively large magnitude of l is consistent with substantial nucleophilic solvent assistance. From Grunwald-Winstein plots the rate data are dissected approximately into contributions from two competing reaction channels. This interpretation is supported for alcohol-water mixtures by the trends of product selectivities, which show a maximum for ethanol-water mixtures. From the KSIE of 1.45 in methanol, it is proposed that the reaction channel favored in methanolwater mixtures and in all less polar media is general-base catalysed and/or is possibly (but less likely) an addition-elimination pathway. Also, the KISE value of 1.35 for DSC in water is expected for SN2-SN1 processes, with minimal general base catalysis, and this mechanism is proposed for solvolyses in the most polar media.

Mechanism of Enzymatic Hydrolysis of Raw Corn Starch by Purified Glucoamylase of $\alpha$-Amylase in an Agitated Bead Reaction System (Glucoamylase 및$\alpha$-Amylase의 분쇄마찰매체 효소반응계에서의 생전분 효소분해 Mechanism)

  • 박동찬;이용현
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.260-267
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    • 1990
  • The mechanism of enzymatic hydrolysis of raw corn starch by the purified glucoamylase and a - amylase in an agitated bead reaction system was studied by investigating the changes of sugar profiles produced by each enzyme, the granular structure of raw corn starch, the amount of enzyme adsorption on residual starch, and the amylose content in residual raw starch. The sugar profiles produced by the action of exo-type glucoamylase or endo-type $\alpha$ -amylase in an agitated bead system were not recognizably differed with those produced in reaction system without bead. Without enzyme the intergenic microcrystalline structure of starch granule was not changed by the simple mechanical impact of solid media, but it was cleaved. However, starch granule was fragment into large number of small particles by the synergistic action of enzyme and attrition-milling media, identified to be the major saccharification enhancing mechanism along with the increased amount of enzyme adsorption. The amylose content decreased more readily in an agitated bead reaction system, especially by $\alpha$ -amylase.

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A Clinical Case Report of Contrast-induced drug eruption patient Treated with Traditional Oriental Medicine (조영제로 유발된 약진(藥疹)환자 치험 1례)

  • Jerng, Ui-Min;Jeong, Jong-Soo;Yoon, Seong-Woo;Park, Jae-Woo
    • Journal of Korean Traditional Oncology
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    • v.12 no.1
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    • pp.75-82
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    • 2007
  • Drug eruption is a cutaneous reaction caused by various drugs. It is a very common drug induced adverse reaction. Contrast media induced drug eruption is rare. But approximately 10% of people injected with contrast media experience adverse reaction, and drug eruption accounts for more than 40% of all adverse reactions. We report a case of mild drug eruption and its treatment process based on korean traditional medicine. The patient is a 50 years old female diagnosed with functional dyspepsia. The patient had gastric discomfort and alternation between diarrhea and constipation. All symptoms showed nearly complete remission with continued korean traditional medical treatment. The patient had been injected with contrast media for Computed tomography(CT) evaluation of her breast cancer history. Rashes appeared on back and abdomen, and urticaria and pruritus appeared on the patient's finger 1 day after injection. We prescribed Goreisan(TSUMURA CO &, TJ-17) and acupuncture on Quchi(LI11), Zusanli(ST36), Yangxi(LI5), Yanggu(SI5). Consequently, the drug eruption showed remarkable improvement. So the author reports korean traditional medicine is effective complementary treatment for drug eruption.

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