• 한국기계가공학회지
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• 제15권6호
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• pp.122-128
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• 2016

The Biosensor biosensor pattern was developed by via an EHD (electro-hydro-dynamics (EHD) patterning process that was performed under atmospheric pressure at room temperature in a single step. The drop diameter was smaller than nozzle diameter and applied high viscosity conductive ink was applied in the EHD patterning method to provide a clear advantage over the piezo and thermal inkjet printing techniques. The Biosensor's biosensor's micro electrode pattern was printed by via a continuous EHD patterning method using 3three- type types of control parameters parameter (input voltage, patterning speed, nozzle pressure). High viscosity (1000 cps) conductive ink with 75 wt% of silver nanoparticles was used for experimentation. The incremental result of impedance of biosensor impedance was measured between the antibody ($10ug{\mu}g/ml$) to spore (0.1 ng/ml, 10 ng/ml, and $1ug{\mu}g./ml$) reaction at frequency 493 MHz frequency.

• 대한기계학회논문집B
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• 제26권5호
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• pp.695-702
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• 2002

Nonlinear dynamics of striped diffusion flames, by the diffusional-thermal instability with Lewis numbers sufficiently less than unity, is numerically investigated by examining various two-dimensional flame-structure solutions. The Lewis numbers for fuel and oxidizer are assumed to be identical and an overall single-step Arrhenius-type chemical reaction rate is employed in the model. Particular attention is focused on identifying the flame-stripe solution branches corresponding to each distinct stripe pattern and hysteresis encountered during the transition. At a Damkohler number slightly greater than the extinction Damkohler number, eight-stripe solution first emerges from one dimensional solution. The eight-stripe solution survives Damkohler numbers much smaller than the extinction Damkohler number until the transition to four-stripe solution occurs at the first forward transition Damkohler number. At the second forward transition Damkohler number, somewhat smaller than the first transition Damkohler number, the transition to two-stripe solution occurs. However, anu further transition from two-stripe solution to one-stripe solution is not always possible even if one-stripe solution can be independently accessed for particular initial conditions. The Damkohler number ranges for two-stripe and one-stripe solutions are found to be virtually identical because each stripe is an independent structure if distance between stripes is sufficiently large. By increasing the Damkohler number, the backward transition can be observed. In comparison with the forward transition Damkohler numbers, the corresponding backward transition Damkohler numbers are always much greater, thereby indicating significant hysteresis between the stripe patterns of strained diffusion flames.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4397-4402
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• 2011

The NF-${\kappa}B$ system of transcription factors plays a crucial role in inflammatory diseases, making it an important drug target. We combined quantitative structure activity relationships for predicting the activity of new compounds and quantitative dynamic models for the NF-${\kappa}B$ network with intracellular concentration models. GFA-MLR QSAR analysis was employed to determine the optimal QSAR equation. To validate the predictability of the $IKK{\beta}$ QSAR model for an external set of inhibitors, a set of ordinary differential equations and mass action kinetics were used for modeling the NF-${\kappa}B$ dynamic system. The reaction parameters were obtained from previously reported research. In the IKKb QSAR model, good cross-validated $q^2$ (0.782) and conventional $r^2$ (0.808) values demonstrated the correlation between the descriptors and each of their activities and reliably predicted the $IKK{\beta}$ activities. Using a developed simulation model of the NF-${\kappa}B$ signaling pathway, we demonstrated differences in $I{\kappa}B$ mRNA expression between normal and different inhibitory states. When the inhibition efficiency increased, inhibitor 1 (PS-1145) led to long-term oscillations. The combined computational modeling and NF-${\kappa}B$ dynamic simulations can be used to understand the inhibition mechanisms and thereby result in the design of mechanism-based inhibitors.

• 대한기계학회논문집B
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• 제38권4호
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• pp.365-371
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• 2014

본 연구에서는 4단 화격자로 구성된 목재 펠릿 보일러의 연소실에 대하여 수치해석을 수행하였다. 목재 펠릿의 화염은 화격자에서 연소실 출구까지 신장되는 데 이 현상이 균질 반응에 기반한 수치해석 기법으로 잘 예측되었다. 수치해석으로 구한 유동장을 보면 연소실 상류에서 출구쪽으로 강한 재순환 유동이 형성되는데 이 유동을 따라 화염이 신장된다. 이와 같은 유동 및 연소 형태는 부분부하 조건에 대하여 수치해석을 수행하였을 때도 유지되었다. 따라서 연소실의 체적을 변경하는 것보다 연소실의 구조를 변경하는 것이 연소 효율을 개선하는 데 도움이 될 것으로 보인다. 본 연구에서는 수치해석 결과를 바탕으로 연소 효율을 높이기 위하여 연소실 출구 위치 변경하거나 화격자 개수를 늘이는 방안 또는 격벽과 같은 내부 구조물을 설치하는 방안을 제안하였다.

• 한국자기공명학회논문지
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• 제20권2호
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• pp.56-60
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• 2016

Adenylate kinase (AK) enzyme which acts as the catalyst of reversible high energy phosphorylation reaction between ATP and AMP which associate with energetic metabolism and nucleic acid synthesis and signal transmission. This enzyme has three distinct domains: Core, AMP binding domain (AMPbd) and Lid domain (LID). The primary role of AMPbd and LID is associated with conformational changes due to flexibility of two domains. Three dimensional structure of human AK1 has not been confirmed and various mutation experiments have been done to determine the active sites. In this study, AK1R138A which is changed arginine[138] of LID domain with alanine[138] was made and conducted with NMR experiments, backbone dynamics analysis and mo-lecular docking dynamic simulation to find the cause of structural change and substrate binding site. Synthetic human muscle type adenylate kinase 1 (hAK1) and its mutant (AK1R138A) were re-combinded with E. coli and expressed in M9 cell. Expressed proteins were purified and finally gained at 0.520 mM hAK1 and 0.252 mM AK1R138A. Multinuclear multidimensional NMR experiments including HNCA, HN(CO)CA, were conducted for amino acid sequence analysis and signal assignments of $^1H-^{15}N$ HSQC spectrum. Our chemical shift perturbation data is shown LID domain residues and around alanine[138] and per-turbation value(0.22ppm) of valine[179] is consid-ered as inter-communication effect with LID domain and the structural change between hAK1 and AK1R138A.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2087-2097
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• 2016

Most cold-adapted enzymes possess higher $K_m$ and $k_{cat}$ values than those of their mesophilic counterparts to maximize the reaction rate. This characteristic is often ascribed to a high structural flexibility and improved dynamics in the active site. However, this may be less convincing to cold-adapted metabolic enzymes, which work at substrate concentrations near $K_m$. In this respect, cold adaptation of a shikimate kinase (SK) in the shikimate pathway from psychrophilic Colwellia psychrerythraea (CpSK) was characterized by comparing it with a mesophilic Escherichia coli homolog (EcSK). The optimum temperatures for CpSK and EcSK activity were approximately $30^{\circ}C$ and $40^{\circ}C$, respectively. The melting points were $33^{\circ}C$ and $45^{\circ}C$ for CpSK and EcSK, respectively. The ${\Delta}G_{H_2O}$ (denaturation in the absence of denaturing agent) values were 3.94 and 5.74 kcal/mol for CpSK and EcSK, respectively. These results indicated that CpSK was a cold-adapted enzyme. However, contrary to typical kinetic data, CpSK had a lower $K_m$ for its substrate shikimate than most mesophilic SKs, and the $k_{cat}$ was not increased. This observation suggested that CpSK may have evolved to exhibit increased substrate affinity at low intracellular concentrations of shikimate in the cold environment. Sequence analysis and homology modeling also showed that some important salt bridges were lost in CpSK, and higher Arg residues around critical Arg 140 seemed to increase flexibility for catalysis. Taken together, these data demonstrate that CpSK exhibits characteristics of cold adaptation with unusual kinetic parameters, which may provide important insights into the cold adaptation of metabolic enzymes.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2148-2158
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• 2016

To investigate the effects of Lactobacillus curvatus and Leuconostoc mesenteroides on suan cai (pickled Chinese cabbage) fermentation, L. curvatus and/or Ln. mesenteroides were inoculated into suan cai. Physicochemical indexes were measured, and the microbial dynamics during the fermentation were analyzed by Illumina MiSeq sequencing and quantitative polymerase chain reaction (qPCR). The results showed that inoculation with lactic acid bacteria (LAB) lowered the pH of the fermentation system more rapidly. The decrease in water-soluble carbohydrates in the inoculated treatments occurred more rapidly than in the control. The LAB counts in the control were lower than in other inoculated treatments during the first 12 days of fermentation. According to the Illumina MiSeq sequencing analyses, Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Cyanobacteria, Fusobacteria, and Verrucomicrobia were present in the fermentations, along with other unclassified bacteria. Generally, Firmicutes was predominant during the fermentation in all treatments. At the genus level, 16 genera were detected. The relative abundance of Lactobacillus in all inoculated treatments was higher than in the control. The relative abundance of Lactobacillus in the treatments containing L. curvatus was higher than in the Ln. mesenteroides-only treatment. The relative abundance of Leuconostoc in the Ln. mesenteroides-containing treatments increased continuously throughout the fermentation. Leuconostoc was highest in the Ln. mesenteroides-only treatment. According to the qPCR results, L. curvatus and/or Ln. mesenteroides inoculations could effectively inhabit the fermentation system. L. curvatus dominated the fermentation in the inoculated treatments.

• 한국항공우주학회지
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• 제44권5호
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• pp.416-422
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• 2016

본 논문에서는 연세대학교 천문우주학과 우주비행제어연구실에서 개발 중인 인공위성 편대비행 테스트베드에 대한 예비 성능분석 결과를 제시하였다. 동역학 모델에 포함되지 않은 동특성과 측정 잡음 등에 의한 불확실성의 영향을 받는 반작용 휠의 응답 성능을 향상시키기 위하여 간단한 1차 선형시스템을 기준 모델로 하는 적응제어기를 설계하였다. 또한 자세 측정값에 잡음이 포함된 환경에서도 원활한 제어를 수행하기 위해 최소제곱법 기반의 실시간 파라미터 추정기법을 이용하여 관성모멘트를 추정하였다. 수치 시뮬레이션과 하드웨어 실험을 통해 설계된 모델 기준 적응제어기의 적합성과 향후 적용가능성을 검토하였고, 전 시간에 걸친 자세 추적오차가 $0.25^{\circ}$ 이내에 머무는 것을 확인하였다. 하지만 하드웨어 실험을 통해 드러난 제어 입력에 대한 데드존의 영향을 줄이기 위해서는 인공위성 시뮬레이터의 설계 변경이 필요하다고 판단된다.

• The Journal of Korean Physical Therapy
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• 제22권2호
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• pp.15-23
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• 2010

Purpose: This study was done to establish reference data for temporo-spatial, kinematic and kinetic parameters for normal Koreans as they age. Methods: Normal adults and children without a previous history of musculoskeletal problems were enrolled in this study. The normal subjects were divided by age into three groups: Group I: children ($11.95{\pm}0.29$ years); Group II: young adults ($23.90{\pm}3.67$ years); Group III: older adults ($71.40{\pm}4.08$ years). The temporo-spatial and kinematic data were measured using 6 MX3 cameras while each subject walked through a 10 m walkway at a self-selected speed. The kinetic data were measured using 2 force plates and were calculated by inverse dynamics. Results: Motion patterns are typically associated with a specific phase of the gait cycle. Our results were as follows: 1. There were significant differences between the different age groups in temporo-spatial parameters such as cadence, double support, time of foot off, stride length, step length, and walking speed. 2. There were significant differences between the groups in kinematic parameters such as range of motion (ROM) of the hip, knee and ankle in the sagittal plane, ROM of the pelvis, hip and knee in the coronal plane and ROM of the pelvis, hip and ankle in the transverse plane. 3. There were significant differences between the groups in kinetic parameters such as joint moments of force, joint mechanical power generation or absorption and ground reaction forces. Conclusion: The results of this study can be utilized (a) as a reference for kinematic and kinetic data of gait analysis in normal Koreans, and (b) as an aide in evaluating and treating patients who have problems relating to gait.

• 한국운동역학회지
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• 제19권4호
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• pp.647-653
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• 2009

본 연구의 목적은 박스 드롭 착지 시 남자와 여자 발레 무용수들의 하지의 생체역학적 요인의 차이를 규명하는 것이다. 본 연구에 참가한 연구 대상자는 20~23세 사이의 여자발레무용수 9명과 21~24세 사이의 남자발레무용수 9명이었다. 박스 드롭 착지 동작은 양 발을 편안하게 벌린 상태에서 연구대상자의 무릎 높이의 박스 위에서 지면반력기 위에 뛰어 내리도록 하였다. 하지의 생체역학적 변인의 계산을 위해서 6대의 고해상도 비디오카메라와 2대의 지면반력기 그리고 8채널 무선 근전도 시스템을 사용하였다. 변인의 차이를 규명하기 위해서 성(2수준, 남자 발레 무용수 여자 발레 무용수)을 독립변인으로 하는 독립 t-test를 실시하였다. 연구결과, 여자발레무용수들은 남자발레 무용수들보다 박스 드롭 착지 시 최대무릎굴곡각도 변인에서 성의 차이가 나타났으나, 나머지 하지의 생체역학적 요인들에서는 성의 차이가 나타나지 않았다. 어린 나이에 점프와 착지 훈련과 교육을 받는 것은 잠재적인 전방십자인대 부상위험 요인을 감소시킨다.