• 대한수의학회지
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• 제21권1호
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• pp.1-6
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• 1981

In order to observe the bactericidal effect of Ranunculus species on pathogenic bacteria, the minimal inhibitory concentration and bactericidal effect was tested Throughout the studies, the following experimental results were obtained and summarized. 1. Minimal inhibitory concentration of Ranunculus species extracts on E. coli was observed in the medium in which 1% Ranunculus species extracts added to brain heart infusion agar. 2. Minimal inhibitory concentration of Ranunculus species extracts on Salmonella species observed in the medium in which 1% Ranunculus species extracts added to brain heart infusion agar. 3. Minimal inhibitory concentration of Ranunculus species extracts on Staphylococcus and Streptococcus was observed in the medium in which 1.5% Ranunculus species extracts added to brain hrart infusion blood agar. 4. The Bactericidal effect of Ranunculus species extracts on E. coli and S. typhi was observed in 30 minutes. 5. The Bactericidal effect of Ranunculus species extracts on staphylococcus aureus was obserded in 40 minutes.

• Journal of Plant Biology
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• 제35권4호
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• pp.425-429
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• 1992

Ranunculus sceleratus 엽병의 세포 신장은 에틸렌에 의하여 촉진되는 것으로 알려져 있다. 오옥신을 처리한 엽병조직 절편에서 spermine은 세포 신장과 에틸렌의 생성을 비슷한 양상으로 억제하였다. Spermine 농도에 대한 오옥신 유도 에틸렌 생성 억제반응은 ACC에 의한 에틸렌 생성의 경우도 유사한 양상을 나타내었으며 이는 폴리아민이 ACC가 에틸렌으로 전환되는 과정을 억제한다는 것을 시사한다. 오옥신 유도 에틸렌 생성은 폴리아민 생합성 억제제인 DFMA아 DFMO에 의하여 각각 현저하게 촉진되었으며 DFMA에 의한 에틸렌 생성의 증가는 spermine을 고농도로 처리하므로써 완전히 소멸되는 결과를 얻었다. 이러한 결과들은 오옥신과 에틸렌에 대한 Ranunculus의 세포성장 반응에서 내생 폴리아민이 조절 역할을 수행한다는 가능성을 입증하는 것이다.

• Plant Resources
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• 제5권1호
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• pp.7-16
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• 2002

The purposes of this study were to clarify of floristic composition and the landscape structure on the habitats of each Ranunculus plant, which were distributed in Daejeon, Korea. Three taxa were distributed and investigated in this study. Floristic composition of habitats were organized by living in flatten open space needed a lot of sunlight and humidity species. Landscape ecological structure of habitats were defined by soils of coarse loamy and loamy skeletal, slope of under 15$^{\circ}$, under the (five percent of woody coverage at 100-300m above the sea levels. Distribution patterns of Ranunculus plants in Daejeon were presented on the 1km X 1km grid map.

• 식물분류학회지
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• 제34권4호
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• pp.335-358
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• 2004

미나리아재비속(Ranunculus)의 털개구리미나리(R. cantonienesis)의 분자계통학적 유연관계를 조사하고 잡종화 가설을 추정하기 위하여, 군외군을 포함한 8분류군의 25개 DNA재료를 대상으로 핵 DNA와 엽록체 DNA의 염기서열을 분석하였다. 털개구리미나리와 근연종에 대하여 maximum parsimony와 maximum likelihood방법으로 분석한 핵 DNA의 ITS 계통수와 psbA-trnH, rps16, trnL 유전자 구간의 염기서열을 조합한 엽록체 DNA 계통수에서, 털개구리미나리는 젓가락나물과 가장 가깝고, 개구리미나리, 왜젓가락나물 순으로 유연관계를 나타내었다. 이러한 분자계통학적 유연관계는 털개구리미나리가 왜젓가락나물과 가장 가깝다는 기존의 외부형태학적 보고와 일치하지 않았다. 염기서열 분석에서 털개구리미나라는 조사된 분류군 중 유일하게 다형성을 나타냄으로서, 염색체수와 핵형에 의하여 보고된 털개구리미나라의 다형성을 지지하였다. 털개구리미나리는 ITS 에서 젓가락나물과 왜젓가락나물의 표지 유전자를 공유하고, 엽록체 DNA에서 왜젓가락나물의 표지 유전자를 공유하였다. 이 결과는 염색체수와 핵형에 의하여 제시되었던 털개구리미나리가 젓가락나물과 왜젓가락나물의 잡종화에 의하여 종분화되었다는 가설을 지지하였으며, 왜젓가락나물이 모계이며, 젓가락나물이 부계로 추정된다.

• 대한수의사회지
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• 제13권6호
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• pp.393-395
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• 1977

Ranunculus species, a poisonous plant that grow in the field and the dales throughout the country has been recommended in folklore for the therapy of traumatic inflammation. A chloroform extract of this plant has been shown to inhibit the growth of severa

• 한국자원식물학회지
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• 제32권6호
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• pp.752-757
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• 2019

The establishment of invasive alien species management is widely recognized as a pivotal issue in the preservation of biodiversity. Ranunculus sardous Crantz, a species native to Europe, has been widely introduced in many other areas of the world, including the coasts of the United States, Australia, China, India, and Japan. In Korea, the first population of this plant was found growing adjacent to a wetland in Hanon, Seogwipo-si, Jeju Province, on 22 May 2018. Field observations confirmed the presences of at least two populations of this species in Jeju Province, Korea. This species is similar to Ranunculus sceleratus L., but can be readily distinguished by its presence of the trichomes in the whole plant, longer petioles of radical leaves, ovate-shaped leaf segments, globose to subglobose-shaped fruits and flat achenes with narrowly winged and papillae. The Invasive Alien Plant Risk Assessment (IAPRA), a system for recognizing and categorizing alien plants in Korea forests, was used to assess the invasiveness status of the species. Based on this system, R. sardous received a low score of 6, suggesting its potential invasion to natural forests. Although the current distribution of R. sardous is restricted to Jeju Province and thus far has had limited impact on local environments, local and regulatory authorities should pay close attention to this plant and take measures to prevent its further expansion.

• Ocean and Polar Research
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• 제38권3호
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• pp.185-193
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• 2016

본 연구에서는 북극 육상식물인 R. hyperboreus를 유기용매로 추출하여 얻어진 추출물에 대하여 세포내 ROS와 peroxynitrite 소거활성을 측정하였다. 추출물의 유의적인 소거활성을 확인한 후에 구성성분의 극성에 따른 소거효과의 변화를 보기 위하여 용매분획을 실시하여 n-hexane, 85% aq.MeOH, n-BuOH, water 분획물을 얻었다. 각 용매분획의 폴리페놀 함량을 측정하였으며 그 결과 n-hexane, water, 85% aq.MeOH, n-BuOH 분획의 순서대로 폴리페놀 함량이 증가하였다. 각 용매분획에 대한 항산화 활성을 검색한 결과 n-hexane 분획의 경우에는 세포생존율에 기인한 어느 정도의 활성변화는 있었으나 대체적으로 소거 활성은 폴리페놀 함량에 비례하였다. 폴리페놀 함량이 가장 높은 n-BuOH 분획물이 가장 좋은 항산화활성을 나타내었으며 그 다음 번째로 폴리페놀 함량이 높은 85% aq.MeOH 분획물이 두 번째로 좋은 항산화활성을 나타내었다.

• 식물병연구
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• 제24권2호
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• pp.138-144
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• 2018

In December 2016, stem rot symptoms were observed on Persian buttercup (Ranunculus asiaticus) plants in Chilgok, Gyeongbuk, Korea. In the early stage of the disease, several black spots appeared on the stem of infected plants. As the disease progressed, the infected stem cleaved and wilted. The causal agent was isolated from a lesion and incubated on Reasoner's 2A (R2A) agar at $25^{\circ}C$. Total genomic DNA was extracted for phylogenetic analysis. Based on the 16S rRNA gene analysis, the isolated strain was found to belong to the genus Pseudomonas. To identify the isolated bacterial strain at the species level, the nucleotide sequences of the gyrase B (gyrB) and RNA polymerase D (rpoD) genes were obtained and compared with the sequences in the GenBank database. As the result, the causal agent of the stem rot disease was identified as Pseudomonas marginalis. To determine the pathogenicity of the isolated bacterial strain, it was inoculated into the stem of healthy R. asiaticus plant, the inoculated plant showed a lesion with the same characteristics as the naturally infected plant. Based on these results, this is the first report of bacterial stem rot on R. asiaticus caused by P. marginalis in Korea.

• 한국식물학회:학술대회논문집
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• 한국식물학회 2001년도 춘계학술발표대회:발표눈문요지록
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• pp.19-19
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• 2001

• Journal of Plant Biology
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• 제37권4호
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• pp.397-402
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• 1994

Possible roles of polyamines in the inhibition of cell elongation in Ranunculus petioles were investigated. Exogenously apoplied polyamines greatly inhibited the auxin-induced petiole growth, while treatment of the tissue with $\alpha$-difluoromethylarginine, the inhibitor of putrescine biosynthesis, further enhanced the growth in the presence of IAA. Inhibitory effect of spermine can also be apparent for fusicoccin-induced elongation, but not for growth induced by a low pH. Spermine also suppressed the ethylene-enhanced growth in the presence of auxin. Using computer-based video digitizer system, the inhibitory effects of spermine on petiole growth were kinetically analyzed. Auxin-induced growth was characterized by an initial and transient growth with a highly elevated rate followed by a steady growth with a slightly reduced rate. Spermine treatment was found to shorten the duration of the initial phase of growth, and to reduce the rates of both the initial and steady growth as well. The latent period for auxin induction was not affected by spermine.