• Korean Journal of Medicinal Crop Science
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• v.14 no.5
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• pp.299-302
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• 2006

Three known triterpenoids were isolated from MeOH extract of C. apiifolia (Ranunculaceae). Their structures were identified as oleanolic acid (1), ursolic acid (2), hederagenic acid (3) by comparison of their physicochemical and spectral data with the literature values. Among them, 2 was isolated for the first time from this plant. The isolated compounds were evaluated for their cytotoxicity against L1210, HL-60, SK-OV-3 tumor cell lines. All compounds 1-3 were shown good activities with $IC_{50}$ values ranging from 7.7 to $25.6\;{\mu}g/ml. This result suggests that triterpenoids 1-3 are main cytotoxic principles of this plant.

• Korean Journal of Pharmacognosy
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• v.33 no.3 s.130
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• pp.157-168
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• 2002

Aconites distribute widely in northern hemispere of the earth reaching to arctic zone from warm and temperate one. Chinese crude drug 'Cho O'(草烏) has been used as a remedy for apoplexy, dyspepsia, neuralgia, chronic rheumatis etc. Though the botanical origin of the crude drug has been considered to be Aconitum species of Ranunculaceae, there has been no confirmation on it. To clarify the botanical origin of Cho O, the morphological and anatomical characteristics of the roots of Aconitum species growing in Korea, i.e. A. chiisanense, A. ciliare, A. jaluense, A. napiforme, A. proliferum, A. pseudoproliferum and A. triphyllum were studied. As the result, Cho O was proved to be the roots of Aconitum triphyllum, A. jaluense, A. chiisanense and A. napiforme.

• Korean Journal of Pharmacognosy
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• v.33 no.3 s.130
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• pp.187-190
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• 2002

Three diterpene alkaloids, songorine (1), neoline (2), N-deethylneoline (3), were isolated from Kyong-Po Buja (Processed Aconitium carmichaeli, Ranunculaceae) which was used as Chinese traditional medicine with analgesic, anti-inflammatory and cardiotonic activity. Their structures were established by chemical and spectroscopic methods.

• Korean Journal of Pharmacognosy
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• v.39 no.3
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• pp.208-212
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• 2008

Seung Ma(升摩) is a famous Chinese crude drug used for treatment of fever, piles and suppurative inflamation. With regard to the botanical origin of Seung Ma, it has been considered to be Cimicifuga spicies of Ranunculaceae, but there has no phrarmacognostical confirmation on it. To clarify the botanical origin of Seung Ma from Korea, the anatomical characteristics of Cimicfuga dahurica, Cimicifuga foetida, Cimicfuga heracleifolia and Cimicifuga simplex were studied. As a result, it was clarified that Seung Ma from Korea was the rhizomes of Cimicifuga dahurica, C, foetida and C, heracleifolia.

• Animal cells and systems
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• v.2 no.4
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• pp.435-438
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• 1998

In the present study, we have examined the crossability of four species in the A. jaluense complex to provide additional information on their delimitation and levels of differentiation. Crosses between diploid A. chiisanense and the tetraploid species including A. jaluense s. str., A. triphyllum, and A. pseudoproliferum yielded no fruit- or seed-set, indicating that the former species is reproductively completely isolated from the latter three species. In interspecific crosses between the tetraploid species, combinations involving A. jaluense s. str. resulted in much reduced fruit- and seed-set, indicating that A. jaluense s. str. is more or less reproductively isolated from the other tetraploid species. However, crossing data revealed the absence of reproductive isolation between A. triphyllum and A. pseudoproliferum These results strongly support the relationships that have been previously suggested for the A. jaluense complex on the basis of morphology, chromosome number, and flavonoid chemistry.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.336-341
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• 1994

For the investigation of medicinal resources in Aconitum species, the studies were carried out to search the less toxic constituents than Aconitum alkaloid in the aerial part of Aconitum jaluense for. album (Ranunculaceae). From the BuOH fraction of MeOH extract, three flavonoid compounds, hyperoside(1), $kaempferol-3-O-{\beta}-_D-galactopyranoside(2)$, $kaempferol-3-O-{\alpha}-_L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-_D-glucopyranoside(3)$ were isolated and identified on the basis of their physico-chemical properties, spectroscopic evidences (UV, IR, NMR and Mass etc.) and comparison with authentics, respectively.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.395-398
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• 1994

A diterpene alkaloid in addition to hypaconitine and mesaconitine was isolated from the tubers of Aconitum napiforme (Ranunculaceae), which is an indigenous plant in Korea. Its structure was elucidated as 8-methoxy 14-benzoyl hypaconine by Physicochemical and spectroscopic data. This compound was isolated from this plant for the first time.

• Korean Journal of Pharmacognosy
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• v.30 no.3
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• pp.301-305
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• 1999

Eight compounds were isolated from the BuOH extract of the stem of Clematis trichotoma (Ranunculaceae). On the basis of spectroscopic evidences, the structures of these compounds were established as rutin, kaempferol 3-O-neohesperidoside, adenosine, adenin, hirustrin, caffeic acid $4-{\beta}-glucoside$, 3-methoxyarbutin and uridine.

• Natural Product Sciences
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• v.17 no.2
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• pp.80-84
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• 2011

Five known compounds, cimigenol (1), 25-O-acetylcimigenol (2), cimigenol 3-O-${\beta}$-D-xylopyranoside (3), ferulic acid methyl ester (4), and visnagin (5), were isolated from Cimicifugae Rhizoma (Ranunculaceae). The chemical structures of these compounds were determined on the basis of spectroscopic analyses including 2D NMR. Compounds 1 - 5 were evaluated for their cytotoxic activity against the HL-60, MCF-7, and A549 cancer cell lines in in vitro. Among them, compounds 4 and 5 showed moderate inhibitory activity against HL-60 cancer cell lines with $IC_{50}$ values of 24.8 and 18.1 ${\mu}M$, respectively.

• Archives of Pharmacal Research
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• v.26 no.9
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• pp.709-715
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• 2003

Aconitum jaluense Komar. (Ranunculaceae) is one of the Aconitum plants growing in Korean peninsula. An investigation of the alkaloidal constituents of this species led to the isolation of seven $C_{19}$-norditerpenoid and a $C_{20}$-diterpenoid alkaloid. Three of them have been identified as neoline, mesaconitine, and hypaconitine, which were isolated from this plant collected from Mt. Bultasan in the north part. The other five alkaloids were determined as lipomesaconitine, lipohypaconitine, 15$\alpha$-hydroxyneoline, hokbusine A, and napelline, which have not been found in this plant. Structures of those alkaloids were determined on the basis of their spectral data. It is of interest to note that a comparison of the present work and the previous report showed some differences in the alkaloidal contents.