• Title/Summary/Keyword: Rana catesbeiana

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Isolation and Characterization of Collagen from Skin of Bullfrog, Rana catesbeiana Shaw

  • Qian, Zhong-Ji;Jung, Won-Kyo;Ngo, Nghiep Dai;Lee, Sang-Hoon;Kim, Se-Kwon
    • Fisheries and Aquatic Sciences
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    • v.10 no.2
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    • pp.53-59
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    • 2007
  • In order to utilize skin of bullfrog (Rana catesbeiana Shaw) as an alternative source of collagen, we investigated and compared biochemical and physical properties of collagens isolated from bullfrog skin. Two kinds of collagen (BSASC; bullfrog skin acid-soluble collagen and BSPSC; bullfrog skin pepsin-solubilized collagen) were isolated by subsequent treatments with acetic acid and pepsin. The amounts of skin collagen isolated in the subsequent treatments were 7.3% BSASC and 18.2% BSPSC on the basis of lyophilized bullfrog skin weight, respectively. According to the electrophoretic pattern and CM-cellulose column chromatogram, the BSASC has the chain composition of ${\alpha}1{\alpha}2{\alpha}3$ heterotrimer, and the BSPSC consists of two ${\alpha}$ chains of ${\alpha}1{\alpha}2$. In addition, the denaturation temperatures of all collagens tested were ranged from $30^{\circ}C\;to\;38^{\circ}C$. This study suggests that there is a possibility to use bullfrog skin collagen as an alternative source of collagen for industrial purposes, and subsequently it may increase the economical value of the bullfrog.

Detection of Infectious Fungal Diseases of Frogs Inhabiting in Korea

  • Kim, Suk;Eom, Ahn-Heum;Park, Dae-Sik;Ra, Nam-Yong
    • Mycobiology
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    • v.36 no.1
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    • pp.10-12
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    • 2008
  • In recent years, there has been a rapid decrease in amphibian populations worldwide, and infectious diseases have been associated with this decline. Diseased frogs inhabiting Korea were collected from fields, and the diseases were identified by morphological and molecular analyses. Two fungal diseases-saprolegniasis and chromomycosis-were detected in the frogs. Saprolegniasis caused by Saprolegnia spp. was found in Rana plancyi chosenica from Gangwon-do and Rana huanrenensis from Chungbuk. Chromomycosis, which is caused by infection with Cladosporium cladosporioides, was detected in Rana catesbeiana from Busan.

Trematodes of the genus Haematoloechus (Digenea: Plagiorchiidae) from frogs in Korea (한국산 개구리류에 기생하는 Haematoloechus속 (Digenea: Plagiorchiidae) 흡충류)

  • Kim, Gi-Hong;Im, Han-Jong;Yun, Il-Byeong
    • Parasites, Hosts and Diseases
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    • v.30 no.4
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    • pp.245-254
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    • 1992
  • Total 242 Rana nigromaculata and g R. catesbeiana were collected from the various localities in Korea from February 1989 to July 1991, and their lungs were examined. Five species in genus Haematoloechus, i. e., H. sibiricus japonicus (Yamaguti, 1936), H. nanchangensis Hsiung, 1934, H. variegatus (Rudolphi, 1819), H. lobatus (Seno, 1907), H. lobatus koreanus, were identified in this study. Among them, H. lebatus koreanus was a new subspecies and H. variegatus and H. lebatus were newly recorded from Korea.

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한국동물학회 1975년 춘계 연구발표회

  • 김헌규
    • The Korean Journal of Zoology
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    • v.18 no.2
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    • pp.102-104
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    • 1975
  • 1971년 5월에 시작한 Rana catesbeiana의 토착화를 위한 실험사육은 성공되었으나 야외에서는 올챙이 성장기가 1년 2개월 걸리고 개구리로 변태한 후에도 3-4년간 성장해야 생식력을 갖게 된다. 이와같이 성장속도가 느린 것은 동면기가 길어서 6개월 동안이나 성장이 중지되기 때문이므로 겨울에도 계속 성장시키기 위해서 실내사육실험을 시도했다. 올챙이는 한 어항에 10마리씩 개구리는 2-4마리씩을 사육했다. 어항의 수온은 $226\\circ C$이상, 실온은 $18^\\circ C-20^\\circ C$로 유지했다. 올챙이는 상추, 실지렁이, 삶은 시금치와 미꾸라지로 사육하였다. 실내사육에서 올챙이의 성장기는 7개월이 단축되었고 개구리는 1년 6개월에 성숙시킬 수가 있었다. 야외와 실내사육을 병행하여 종와를 생산하면 우리나라에서도 개구리 산업을 개발할 수가 있을 것으로 전망된다.

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Anuran Metamorphosis: a Model for Gravitational Study on Motor Development

  • Jae Seung;Jin Cheul;In-Ho;Park, In-Ho
    • Animal cells and systems
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    • v.4 no.3
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    • pp.223-229
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    • 2000
  • Limbs and supporting structures of an organism experience a full weight of its own when it lands from water, because neutral buoyancy in the aquatic habitat will be no longer available in the terrestrial world. Metamorphosis of anuran amphibians presents 8 good research model to examine how this transition from non-loading to weight-loading affects development of motor capacity at the time of their first emergence on land. Our video analysis of the transitional anurans, Rana catesbeiana, at Gosner stage 46 (the stage of complete transformation) demonstrated that the take-off speed increased 1.23-fold after the first six hours of weight-loading on the wet ground. It did not increase further during the following three days of loading, and was close to the level of mature frogs with different body mass. During development of larvae in deep water with no chance of landing through metamorphosis, both tension and power of a hindlimb anti-gravity muscle increased 5-fold between stages 37 and n. However, the muscle contractility increased more rapidly when the larvas could access the wet ground by their natural landing behavior after stages 41-42. Muscle power, one of major factors affecting locomotory speed, was 1.29-fold greater in the loaded than in the non-loaded larvae at the transitional stage. Thus, weight-loading had a potentially significant effect on the elevation of motor capacity, with a similar extent of increment in locomotory speed and muscle power during the last stages of metamorphosis. Such a motor adjustment of the froglets in a relatively short transitional period would be important for effective ecological interactions and survival in their inexperienced terrestrial life.

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