• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.352-355
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• 2003

To investigate the protective effect of Gamdu-tang(GDT) and its constituents. Radix Glycyrrhizae(RG) and Semen Glycine(SG) on the damage of cardiac endothelial cells by xanthine oxidase (XO)/hypoxanthine (HX)-induced oxygen free radical, Neutral Red (NR) and DNA synthesis assay were used. The results were obtained as follows ; Cardiac endothelial cells treated with XO/HX showed the cytotoxicity such as decreases in viability and DNA synthesis. Cardiac endothelial cells pretreated with GDT extracts were not showed the decrease of DNA synthesis by XO/HX, These results show that XO/HX elicits toxic effects in cultured cardiac endothelial cells derived from neonatal rat, and suggest that GDT extract is very effective in the prevention of XO/HX-induced toxicity.

• Journal of Ginseng Research
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• v.24 no.4
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• pp.202-205
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• 2000

KJHST (Kejihongsarntang) is a modified oriental prescription that consists of five herbs such as Ginseng Radix rubra Koreana, Atractylodis Rhizoma, Zingiberis Rhiaoma, Cinnamomi Ramulus and Glycyrrhizae Radix. For the evaluation of antioxidant and staminal activities of KJHST (Kejihongsarntang), the study was done in comparison of Ginseng Radix rubra (GR). For the antioxidant study, KJHST inhibited hemolysis of erythrocyte and decolored the DPPH(2,2-diphenyl-1-picrylhydrazyl) free radical in a dose depenrlent manner more effectively than GR alone in vitro. KJHST and GR significantly suppressed the time course (1 hr∼6 hr)-level of MDA (malondialdehyde) following AAPH (2,2'-azo-bis-(2-amidino-propane) dihydrochloride) treatment in vivo as compared with control data with no statistical difference. From the evaluation of stamina by swimming test GR and KJHST significantly increased the swimming time in a time and dose dependent manner as compared with control data, while GR was more effective than KJHST in 2 weeks after treatment, though KJHST was more effective than GR at low dose (25 m/kg) 4 weeks after treatment. From the results it can be concluded GR and KJHST had antioxidant and staminal activities.