• 대한핵의학회:학술대회논문집
• /
• 1973.11a
• /
• pp.52.3-52.3
• /
• 1973

• 대한핵의학회:학술대회논문집
• /
• 1977.05a
• /
• pp.92.2-92.2
• /
• 1977

• 대한핵의학회:학술대회논문집
• /
• 1977.05a
• /
• pp.95.1-95.1
• /
• 1977

• 대한핵의학회:학술대회논문집
• /
• 1979.05a
• /
• pp.21.1-21.1
• /
• 1979

• The Korean Journal of Nuclear Medicine
• /
• v.21 no.1
• /
• pp.1-3
• /
• 1987

• 대한핵의학회:학술대회논문집
• /
• 1971.11a
• /
• pp.78.3-78.3
• /
• 1971

• Journal of Food Hygiene and Safety
• /
• v.10 no.2
• /
• pp.61-64
• /
• 1995

The present study was conducted to examine the effect of recombinant bovine somatotrpin(${\gamma}$BST), which was administered to cow to promote milk production, on bST levels in milk. Fourteen cows were divided cows were divided into two groups: 1) control cows received neither ${\gamma}$bST nor vehicle, 2) treated cows were administered twice at two-week interval with 500 mg ${\gamma}$bST each cow byj after lst injection. Milk samples were taken on day 0 (prior to injection), day 7 (7 days after lst injection), day 21 (7 days after 2nd injection) and day 35 (21 days after 2nd injection). Milk bST concentration was measured by the radioimmunoassay method. There was no statistical difference(p<0.05) in milk bST levels between two groups showing bST levels in the range of 1.8 ng/m/ to 3.1 ng/m/. That is, ${\gamma}$bST administration did not increase bST levels in milk.

• Nuclear Engineering and Technology
• /
• v.10 no.4
• /
• pp.223-229
• /
• 1978

A typical abnormal standard dose response curve of nearly convex form. often encountered in insulin radioimmunoassay (IRIA) has been analyzed by varying incubation conditions. The cause of such abnormality has been turned out to be the results of an incomplete equilibrium between the two reactants. By careful control of the temperature of serum sample an immediate cause of sharp deviation of B/F value of tile sample tube from the measurable range in the standard curve has also been investigated. The two main troubles have been proven to be stemed from incubation conditions. Incubation at 4$^{\circ}C$ for 48 hrs is emphasized for IRIA.

• BMB Reports
• /
• v.32 no.5
• /
• pp.474-479
• /
• 1999

Using a hybridoma technique, spleen cells of Balb/c mice immunized with human chorionic gonadotropin (hCG) were fused with NS-1 mouse myeloma cells. Two hybrid cell lines, clones KS-8 and KS-19, secreting monoclonal antibodies to hCG, were isolated. KS-8 and KS-19 belong to the immunoglobulin $G_1$ subclass. With the aid of a double-antibody radioimmunoassay, it was established that the KS-8 monoclonal antibody recognizes an immunodeterminant of the $\beta$-subunit of hCG, whereas the KS-19 monoclonal antibody recognizes an epitope present on the $\alpha$-subunit of hCG. The KS-8 monoclonal antibody specifically reacts with human chorionic gonadotropin and shows cross-reactivity of less than 0.3% to other related human glycoprotein hormones. On the other hand, using a hemagglutination test based on antibody-induced agglutination of sheep red blood cells coated with hCG, It was shown that only the KS-19 monoclonal antibody was capable of inducing a positive reaction, although both monoclonal antibodies had similar binding capacity to the coated cells. The results from the dual screening procedures demonstrate that KS-8 and KS-19 monoclonal antibodies show high sensitivity in two different assays, and are hence useful for the qualitative and quantitative determination of hCG by both radioimmunoassay and hemagglutination inhibition tests.

• BMB Reports
• /
• v.30 no.6
• /
• pp.443-447
• /
• 1997

We have developed a novel method for assays of spermidine and spermine synthase (aminopropyltransferase) activities using antibody against 5'-deoxy-5'-methylthioadenosine (MTA). A new assay is reported here which is based on the observation that MTA is formed as a stoichiometric by-product of the spermidine and spermine synthases reactions. In order to determine MTA, a radioimmunoassay method with sensitivity and rapidity was used. (Lee and Cho, 1997). In this assay, adenine must be added in the reaction mixture, since it effectively inhibits the action of MTA phosphorylase by which MTA is metabolized. This assay is a improvement in term of sensitivity and time saving, compared to the currently used methods. It has a level of sensitivity (100 fmol) sufficient to monitor aminopropyltransferase activities in incubations containing as little as $10{\mu}g$ protein prepared from rat tissue homogenate. The results obtained showed that this method is particularly useful for cultured cells with low enzyme concentration. Moreover, this assay has the advantage which allows studies using alternative substrates (other amines). Spermidine synthase activity was high in rat liver, but low in rat kidney. The activity of spermine synthase was in most rat tissues very low as compared to that of spermidine synthase, but was high in brain.