• Elastomers and Composites
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• v.48 no.4
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• pp.269-275
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• 2013

In this study, we prepared poly(ethylene-ter-1-hexene-ter-divinylbenzene) using various metallocene catalysts with trityl tetrakis(pentafluorophenyl)borate/triisobutylaluminium cocatalysts system. We tried rac-$Et(Ind)_2ZrCl_2$, rac-$SiMe_2(Ind)_2ZrCl_2$, and rac-$SiMe_2(2-Me-Ind)_2ZrCl_2$ to choose optimum metallocene catalyst, comparing with catalytic activity, molecular weight, molecular weight distribution of the terpolymers. To study the effects of chain length of high ${\alpha}$-olefins on the terpolymerization, we synthesized the terpolymers using 1-hexene, 1-octene, 1-decene or 1-dodecene. We characterized chemical composition, thermal properties, and mechanical properties of the terpolymers.

• International Journal of Concrete Structures and Materials
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• v.10 no.1
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• pp.61-73
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• 2016

In predicating the probability distribution of chloride diffusion coefficient of recycled aggregate concrete ($D_{RAC}$), the morphological characteristics of three phases, i.e., the old attached mortar, the natural aggregate and the new mortar, should all be taken into account. The present paper attempts to develop a probability density evolution method (PDEM) to achieve this. After verifying the derived PDEM results with experimental results, the effects of old attached mortar to the $D_{RAC}$ are examined in a quantitative manner. It is found that (1) the variation of the attached mortar content is much sensitive to $D_{RAC}$; (2) given the probability distribution of the content and chloride diffusion coefficient of old mortar, the probability distribution of DRAC can be analysed based on the PDEM; and (3) the critical chloride diffusion coefficient at a certain assurance rate can be obtained by the PDEM. The analysis results of this investigation will be valuable to the durability design for RAC.

• Polymer(Korea)
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• v.25 no.4
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• pp.468-475
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• 2001

For copolymerization of ethylene and norbornene initiated by various metallocene catalysts such as $rac-Et(Ind)_2ZrCl_2,\;rac-Me_2Si(Ind)_2ZrCl_2,\;rac-Me_2Si(Cp)_2ZrCl_2,\;and\;(n-BuCp)_2ZrCl_2$ with modified methylaluminoxane(MMAO) cocatalyst, the ${\alpha}$-olefins such as 1-hexene(H), 1-octene and 1-decene were added as a 3rd monomer. In this situation, the effects of the polymerization condition, the catalyst structure as well as the structure and the amount of added ${\alpha}$-olefin on the catalyst activity as well as the properties and structure of polymer were examined. As results, it was found that the catalyst activity and thermal property of polymer depended on not only catalyst structure but also ${\alpha}$-olefin structure. For $rac-Et(Ind)_2ZrCl_2/MMAO$ catalyst system, it was possible to get high activity and controllable $T_g$ of polymer. Among ${\alpha}$-olefins, H as a 3rd monomer exhibited the maximum enhancement in catalyst activity.

• Experimental and Molecular Medicine
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• v.51 no.2
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• pp.9.1-9.10
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• 2019

Mesangial cell proliferation has been identified as a major factor contributing to glomerulosclerosis, which is a typical symptom of diabetic nephropathy (DN). Lysophosphatidic acid (LPA) levels are increased in the glomerulus of the kidney in diabetic mice. LPA is a critical regulator that induces mesangial cell proliferation; however, its effect and molecular mechanisms remain unknown. The proportion of ${\alpha}-SMA^+/PCNA^+$ cells was increased in the kidney cortex of db/db mice compared with control mice. Treatment with LPA concomitantly increased the proliferation of mouse mesangial cells (SV40 MES13) and the expression of cyclin D1 and CDK4. On the other hand, the expression of $p27^{Kip1}$ was decreased. The expression of $Kr{\ddot{u}}ppel$-like factor 5 (KLF5) was upregulated in the kidney cortex of db/db mice and LPA-treated SV40 MES13 cells. RNAi-mediated silencing of KLF5 reversed these effects and inhibited the proliferation of LPA-treated cells. Mitogen-activated protein kinases (MAPKs) were activated, and the expression of early growth response 1 (Egr1) was subsequently increased in LPA-treated SV40 MES13 cells and the kidney cortex of db/db mice. Moreover, LPA significantly increased the activity of the Ras-related C3 botulinum toxin substrate (Rac1) GTPase in SV40 MES13 cells, and the dominant-negative form of Rac1 partially inhibited the phosphorylation of p38 and upregulation of Egr1 and KLF5 induced by LPA. LPA-induced hyperproliferation was attenuated by the inhibition of Rac1 activity. Based on these results, the Rac1/MAPK/KLF5 signaling pathway was one of the mechanisms by which LPA induced mesangial cell proliferation in DN models.

• Korean Chemical Engineering Research
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• v.46 no.1
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• pp.137-141
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• 2008

Copolymerization of ethylene and ${\alpha}$-olefin using $rac-Et[Ind]_2ZrCl_2/MAO$ catalyst embedded onto polysty-rene was examined. The embedded catalyst was prepared by polymerizing a small amount of styrene with $rac-Et[Ind]_2ZrCl_2$. The catalytic activities of the embedded catalyst were higher than those of the homogeneous catalystregardless of comonomer type and the characteristic of the active sites of the embedded catalyst was not affected duringthe embedding process. Based on the DSC and NMR analyses of the produced copolymers, it was thought that theembedded catalyst had similar or slightly better comonomer incorporation ability. Furthermore, the copolymers produced by the embedded catalyst had higher bulk densities and better particle morphology than those by the homogeneous catalyst.

• Molecules and Cells
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• v.37 no.4
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• pp.314-321
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• 2014

CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3'-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.

• Proceedings of the IEEK Conference
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• 2000.11a
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• pp.189-192
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• 2000

This paper proposes a fuzzy-based random access controller with a superimposed frame structure (F$^2$RAC) fur voice/data-integrated wireless networks. F$^2$RAC adopts mini-slot technique for reducing contention cost, and these mini-slots of which number may dynamically vary from one frame to the next as a function of the traffic load are further partitioned into two regions for access requests coming from voice and data traffic with their respective QoS requirements. And F$^2$RAC is designed to properly determine the access regions and permission probabilities for enhancing the data packet delay while ensuring the voice packet dropping probability constraint. It mainly consists of the estimator with Pseudo-Bayesian algorithm and fuzzy logic controller with Sugeno-type of fuzzy rules. Simulation results prove that F$^2$RAC can guarantee QoS requirement of voice and provide the highest throughput efficiency and the smallest data packet delay amongst the different alternatives including PRMA［1］, IPRMA［2］, and SIR［3］.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6305-6309
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• 2013

Background: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. Materials and Methods: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. Results: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down-regulate the expression of MMP-3, Twist and Snail. Conclusions: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.10a
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• pp.144-144
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• 2003

• Steel and Composite Structures
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• v.49 no.1
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• pp.91-107
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• 2023

An effective approach to promoting sustainability within the construction industry is the use of recycled aggregate concrete (RAC) as a substitute for natural aggregates. Ensuring the frost resilience of RAC technologies is crucial to facilitate their adoption in regions characterized by cold temperatures. The main aim of this study was to use the Random Forests (RF) approach to forecast the frost durability of RAC in cold locations, with a focus on the durability factor (DF) value. Herein, three optimization algorithms named Sine-cosine optimization algorithm (SCA), Black widow optimization algorithm (BWOA), and Equilibrium optimizer (EO) were considered for determing optimal values of RF hyperparameters. The findings show that all developed systems faithfully represented the DF, with an R² for the train and test data phases of better than 0.9539 and 0.9777, respectively. In two assessment and learning stages, EO - RF is found to be superior than BWOA - RF and SCA - RF. The outperformed model's performance (EO - RF) was superior to that of ANN (from literature) by raising the values of R² and reducing the RMSE values. Considering the justifications, as well as the comparisons from metrics and Taylor diagram's findings, it could be found out that, although other RF models were equally reliable in predicting the the frost durability of RAC based on the durability factor (DF) value in cold climates, the developed EO - RF strategy excelled them all.