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http://dx.doi.org/10.14348/molcells.2014.2360

Inhibition of Cell Proliferation and Migration by miR-509-3p That Targets CDK2, Rac1, and PIK3C2A  

Yoon, Sena (Graduate School of Biotechnology, Kyung Hee University)
Han, Eunji (Graduate School of Biotechnology, Kyung Hee University)
Choi, Young-Chul (Graduate School of Biotechnology, Kyung Hee University)
Kee, Honghwan (Graduate School of Biotechnology, Kyung Hee University)
Jeong, Yongsu (Graduate School of Biotechnology, Kyung Hee University)
Yoon, Jaeseung (Graduate School of Biotechnology, Kyung Hee University)
Baek, Kwanghee (Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Molecules and Cells / v.37, no.4, 2014 , pp. 314-321 More about this Journal
Abstract
CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3'-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.
Keywords
CDK2; growth inhibition; microRNA; miR-509-3p; PIK3C2A; Rac1;
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