• Journal of fish pathology
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• v.19 no.2
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• pp.183-188
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• 2006

In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.3
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• pp.223-231
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• 2001

For the purpose of providing the basic data on the improved fishing gear and the man power saving, which contribute to enhance fishing efficiency of set net in the coast of Jeju Island, this study executed the test of fish tank by reducing these actual nets by 1/30 and using the manufactured model nets, and observed and interpreted the behaviors of entering and escaping of the schools of mackerel Scomber japonicus, horse mackerel Trachurus japonicus and rabbit fish Siganus fuscescens. Thereby the result is as follows ; 1. After the elapsed time of 60 seconds, in case of rectangular set net, the ratio for entering by a school of fish marked 50% with mackerel, 18% with horse mackerel and 28% with rabbit fish, and in case of pound net, the ratio for entering net by a school of fish marked 70% with mackerel, 60% with horse mackerel and 30% with rabbit fish. 2. After the elapsed time of 60 seconds, in case of rectangular set net, the ratio for escaping by a school of fish marked 70% with mackerel, 40% with horse mackerel and 24% with rabbit fish, and in case of pound net, the ratio for escaping from net by a school of fish marked 0% with mackerel, 0% with horse mackerel and 3% with rabbit fish. 3. After the elapsed time of 60 seconds, in case of rectangular set net, the ratio for remaining by a school of fish marked 30% with mackerel, 60% with horse mackerel and 76% with rabbit fish, and in case of pound net, the ratio for remaining by a school of fish marked 100% with mackerel, 100% with horse mackerel and 97% with rabbit fish.

• Environmental Analysis Health and Toxicology
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• v.12 no.3_4
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• pp.15-22
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• 1997

This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.

• Environmental Analysis Health and Toxicology
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• v.11 no.1_2
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.4
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• pp.349-355
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• 2019

Reproductive ecology of rabbit fish, Siganus fuscescens were investigated from samples collected monthly in the coastal waters off Jeju Island of Korea from February to December 2014. In male and female of S. fuscescens, the values of GSI were reached high values between July to August in which the water temperature was high, and then decreased until December. The occurrence of individuals which have mature eggs in ovary were higher from July to August. Therefore, a main spawning season was estimated from July to August, with the highest peak in July. Throughout histological observation of the ovary in the spawning period, S. fuscescens belong to an group-synchronous and has a one spawning season a year. Fecundity of ovary ranged from 380,000 to 2,600,000 during the spawning period. Size at 50% sexual maturity, determined from logistic equation, were 25.1 cm TL which is over two years.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.2
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• pp.169-175
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• 2014

The age and growth of rabbit fish, Siganus fuscescens were investigated from samples monthly collected in the coast of Jeju island, Korea from February to December 2012. Ages were determined from annuli in otoliths, and annuli were formed in one or two months immediately after spawning once a year. Also, main spawning period was estimated between July and August, thus rings were considered as annual marks. The von Bertalanffy growth equations estimated from a non-linear regression method were $L_t=37.24(1-e^{-0.33(t+1.24)})$ ($R^2=0.92$) for female and $L_t=31.44(1-e^{-0.45(t+1.23)})$ ($R^2=0.90$) for male, and the growth between female and male was different.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.3
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• pp.232-239
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• 2001

For the purpose of providing the basic data on the improved fishing gear and the man power saving, which contribute to enhance fishing efficiency of set net in the coast of Jeju Island, this study analyzed the catch of two fishing place for the past 3 years in order to compare the fishing efficiency between rectngular set net and pound net, which have been used for fishing with being attached to the set net fishing place in coast of Jeiu Island, Thereby the result is as follows ; 1. When using pound net, the total amount of catch during 1997 to 1999 was 2 times more than that of when using rectangular set net. 2. When using Pound net, the catch of squid, mackerel, rabbit fish and Yellow tail during 1997 to 1999 was 1.8 times more than that of when using rectangular set net.. 3. In case of rectangular set net, CPUE marked 10.1㎏ with horse amckerel, 20.5㎏ with squid, 18.0㎏ with rabbit fish and 2.2㎏ with Yellow tail, and in case of pound net, CPUE marked 57.5 ㎏ with horse mackerel, 30.0㎏ with squid, 25.0 with rabbit fish and 4.7㎏ with yellow tail, and on the whole CPUE of poind net marked higher. 4. In case of rectangular set net, the catch ratio for fishing operation marked 64% with horse mackerel, 79% with squid, 39% with rabbit fish and 14% with yellow tail, and in case of pound net, the catch ratio fishing operation marked 18% with horse mackerel, 85% with souid 40% with rabbit fish and 14% with yellow tail, and accordingly it showed the result that the catch ratio for fishing operation was higher with the case of rectangular set net, but higher with the case of pound net.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.620-628
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• 1996

To avoid the self-agglutination of Staphylococcus aureus sensitized with rabbit antibody in the absence of antigen, we determined the optimum concentration of rabbit antibody for sensitization. It was analyzed by using three different kinds of S. aureus strains at various concentraions of antibody. The optimized coagglutination test using the S. aureus sensitized with rabbit antibody was applied to the diagnosis of edwardsiellosis in field and in laboratory. The presence of E. tarda as low as $10\;{\mu}g/ml$ was detected by this method. Moreover, it showed good coagglutination results against several different forms of antigens such as FKC, EDTA or heat extracted antigen of E. tarda. E. tarda strains, isolated from the flounders suffering from edwardsiellosis in fields, showed some cross-reactions to the E. tarda 219 analyzed by both agglutination and coagglutination test with rabbit anti-E, tarda 219 antibody. The degree of cross-reactions analyzed was enough to apply the coagglutination test for the diagnosis of edwardsiellosis in field. Thus, even 1,000 fold diluted tissue homogenate of infected flounder naturally contained enough E. tarda as an antigen to show good coagglutination with S. aueus sensitized with rabbit anti-E, tarda 219 antibody. The successful application of this method to the homogenate and heat extract of tissues from naturally or artificially infected flounder or tilapia preyed that coagglutination test was a simple and rapid reliable dignostic technique suitable for using in laboratory and field without any special equipments.

• Journal of Food Hygiene and Safety
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• v.10 no.2
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• pp.73-79
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• 1995

Incidents of seafood and massive fish kills have been rapidly increasing in both frequency and geographical distribution and the socioeconomic impacts brought by those incidents. However, the biological origins of those marine toxins have not been well clarified. Most of the marine organisms investigated are filter-feeder, which accumulate toxins from their food and/or their symbiotic microalgae. We have examined the action on rabbit platelets of marine toxins isolated from cultured dinoflagellates and sponge collected at Okinawa. Maitotoxin (MTX) is a water-soluble toxin isolated from the cultured dinoflagellate Gambierdiscus toxicus which causes a seafood poisoning in tropical regions. Zooxanthellatoxin A (ZT-A) was isolated from exteracts of cultured symbiotic dinoflagellate Symbiodinium sp. (socalled zooxanthella) from flatworms of the genus Amphiscolops collected at Okinawan marine sponge Theonella sp. MTX caused a disaggregation and a dissolution of large aggregates. ZT-A caused a dissolution of small aggregates followed by a increment of light tranmission. TZ-A caused an initial and transient shape change followed by a sustained aggregation and a increment of large aggregates. In conclusion, marine toxins exert unique patterns on the light trasmission and the size of aggregates in rabbit platelets by their concentrations and kinds

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.57 no.1
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• pp.10-24
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• 2021

A seasonal characteristic of fish assemblages in the coastal waters of Gapa-do was determined using samples collected by a trammel net from 2015 to 2019. Annual mean temperature and salinity were 18.9℃, 33.6 psu (2015), 19.5℃, 33.6 psu (2016), 18.9℃, 33.8 psu (2017), 18.8℃, 34.5 psu (2018) and 19.5℃, 33.7 psu (2019), which show a stable water mass. During the survey period, a total of 50 species (30 families and 8 orders) of fishes were identified, including Scorpaeniformes (3 families and 9 species) and Perciformes (18 families and 29 species) that accounted for 68% of the total number. The number of species was higher in summer than in winter, showing the peak in the summer by 35 species and the low in the winter by 20 species. The number of individuals and the biomass peaked in the summer by 690 individuals and 151.4 kg, and were the lowest in the autumn by 336 individuals and 76.6 kg. The annual diversity index, evenness index and richness index were appeared to be 2.54-2.75, 0.68-0.76 and 5.32-6.65 at each stations. The dominance index was appeared to be the highest in 2018 and the lowest in 2015. Marbled rockfish (Sebastiscus marmoratus), rock fish (Sebastes inermis), filefish (Stephanolepis cirrhifer), rabbit fish (Siganus fuscescens), porcupine fish (Diodon holocanthus) and smallscale blackfish (Girella melanichthys) were dominant species, which occurred abundantly during the entire survey period. Subtropical fishes were identified as a total of 24 species (48.0%) and the number of species was higher in 2019 by 20 (60.6%) species and lower in 2015 by 13 (44.8%) species. The dominant species of the subtropical fishes were appeared to be rabbit fish, porcupine fish, smallscale blackfish, flag fish (Goniistius zonatus), stripey (Microcanthus strigatus), grunter (Parapristipoma trilineatum), and bluestriped angelfish (Chaetodontoplus septentrionalis) at the study sites.