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Anti-aging Effect and Gene Expression Profiling of Aged Rats Treated with G. bimaculatus Extract

  • Ahn, Mi Young;Hwang, Jae Sam;Yun, Eun Young;Kim, Min-Ji;Park, Kun-Koo
    • Toxicological Research
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    • v.31 no.2
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    • pp.173-180
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    • 2015
  • Extract from Gryllus bimaculatus crickets inhibits oxidation at the DNA level, with reduced production of 8-hydroxy-2'-deoxyguanosine (8-OHdG). Microarray analyses were performed with a rat 28K cDNA clone set array to identify the gene expression profiles of aged (10 months old) Wistar Kyoto rats treated for one month with 100 mg/kg G. bimaculatus ethanol extract to assess the effects. The extract produced a meaningful anti-edema effect, evident by the inhibition of creatinine phosphokinase activity. The weights of abdominal and ovarian adipose tissues were reduced and the proportion of unsaturated fatty acids in adipose tissues was increased in an extract dose-dependent manner. Compared with untreated control rats, rats treated with the extract displayed the upregulation of 1053 genes including Fas (tumor necrosis factor receptor superfamily, member 6), Amigo3 (adhesion molecule with an immunoglobulin-like domain), Reticulon 4, 3-hydroxy-3-methylglutaryl-coenzyme (Hmgcr; a reductase), related anti-fatigue (enzyme metabolism), and Rtn antioxidant, and the downregulation of 73 genes including Ugt2b (UDP glycosyltransferase 2 family), Early growth response 1, and Glycoprotein m6a. Data suggest that G. bimaculatus extract may have value in lessening the effects of aging, resulting in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes.

Specifics in Weed Competition and Herbicide Response of Chinese Cabbage(Brassica pekinensis) under Foggy Condition (안개에 기인하는 배추(Brassica pekinensis)의 잡초경합(雜草競合) 및 제초제반응(除草劑反應) 특이성(特異性))

  • Guh, J.O.;Park, T.D.;Chon, S.U.;Kuk, Y.I.
    • Korean Journal of Weed Science
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    • v.15 no.4
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    • pp.270-277
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    • 1995
  • The research was carried out in tray in greenhouse equipped with Auto Foggy Systems(SAE KI RIN Co.) and the results were summarized as follows. 1. The number of leaves and fresh weight of Chinese cabbage were not different, but the plant height was increased due to foggy condition. 2. Plant height was decreased in 40 and 60 days of weed competition by foggy condition, the number of leaves was not different. And, shoot fresh weight was decreased by in all the duration of weed competition under foggy condition. Especially, that was greater in 40 days. 3. The shoot fresh weight was decreased by pendimethaline and napropamide application under foggy condition. Also, the number of leaves was decreased at 60 days after transplanting by pendimethaline application under foggy condition. 4. Pendimethalin, napropamide and alachlor treatments were not different in the weeding efficacy between non-foggy and foggy conditions. Trifluraline showed lower weeding efficacy by seedling emergence of large crabgrass as the days of treatment got longer under foggy condition.

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Weed Competition and Herbicide Response of Rice under the Foggy Condition II. Growth and Weed Competition of Rice (안개조건하(條件下)에서 벼(Oryza sativa L.)의 잡초경합(雜草競合)과 제초제(除草劑) 반응차이(反應差異)에 관(關)한 연구(硏究) II. 벼의 생장(生長)과 잡초경합(雜草競合))

  • Guh, Ja-Ock;Lee, Min-Soo;Kuk, Yong-In;Chon, Sang-Uck
    • Korean Journal of Environmental Agriculture
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    • v.14 no.2
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    • pp.221-231
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    • 1995
  • The objectives of the present study were to find out the differences in growth and weed competition of rice under the foggy and non-foggy condition, and finally, the fundamental data for the establishment of the paddy weed control system under the locational foggy regions. The research was carried out on tin trays ($0.12m^2$) in greenhouse equipped with an Auto Foggy System(SAE KI RTN Co.). The results are summarized as follows: Exp. I. Difference in rice growth under the foggy and non-foggy condition. 1. While the plant height was not affected, the number of tillers was decreased by the foggy condition. The ratio of the number of effective tiller, however, became higher under the foggy condition. 2. Due to the fog present, the heading rate was decreased and heading time was delayed. 3. The foggy condition did not affect the dry weight of rice straw whether they were grown under the foggy or non-foggy condition. However, yield components such as the number of ears, the number of grains per ear, thousand kernel weight and percentage of ripeness were reduced. Particularly, weights of perfect brown and unpolished rice were also decreased. Exp. II. Effect of the duration of competition between weed and rice grown under the foggy condition on the growth of rice plant. 1. There was no difference in the height of rice grown under a different duration of competition. There was a clear tendency that the number of tillers of rice grown under the foggy condition was decreased as the duration of competition was decreased. 2. When the duration of competition was longer than 60 days, the heading rate was decreased and the initiation of heading was also delayed by 2-4 days. 3. Under the foggy condition, the duration of competition for more than 40 days affected dry weight of rice straw and the difference in yield was greatest in the non-competition and competition for more than 40 days.

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Weed Competition and Herbicide Response of Rice under the Foggy Condition;I. Rice Response to Herbicides (안개 조건하(條件下)에서 벼(Oryza sativa L.)의 잡초경합(雜草競合)과 제초제(除草劑) 반응차이(反應差異)에 관(關)한 연구(硏究);I. 벼의 제초제(除草劑) 반응(反應))

  • Guh, Ja-Ock;Kuk, Yong-In;Kwon, Oh-DO
    • Korean Journal of Environmental Agriculture
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    • v.14 no.1
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    • pp.88-96
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    • 1995
  • The research was carried out in tray ($0.12m^2$) in greenhouse ($30{\pm}2^{\circ}C$${\sim}$$25{\pm}2^{\circ}C$) equipped with Auto Foggy System (SAE KI RIN Co.) and results are summarized as follows: 1. Bifenox and bensulfuron retarded the growth of rice under foggy condition and the weeding efficacy among the herbicides tested was not different. 2. Weeds under foggy condition emerged at smaller amounts than under the non-foggy condition. bensulfuron, molinate and bifenox showed the high weeding efficacy. There was a tendency that molinate reduced the number of tillers at 20 and 60 days after transplanting. 3. Among the herbicides tested, only the molinate reduced the dry weight and yield of rice significantly.

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Specifics in Weed Competition and Herbicide Response of Radish(Raphanus sativus L.) Under Foggy Condition (안개에 기인하는 무우(Raphanus sativus L.)의 잡초경합(雜草競合) 및 제초제반응(除草劑反應) 특이성(特異性))

  • Guh, J.O.;Kim, S.C.;Kuk, Y.I.;Chon, S.U.
    • Korean Journal of Weed Science
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    • v.15 no.3
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    • pp.197-205
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    • 1995
  • The objectives of the present study were to find out 1)the differences in growth of radish under foggy and non-foggy condition, 2)the weed competition between radish and major upland weeds including Echinochloa crus-galli, 3)difference in response of radish to herbicide and weed, and finally 4)the fundamental data for the establishment of radish weed control system under locational foggy regions. The research was carried out by tray in greenhouse equipped with Auto Foggy System(SAE KI RIN Co.) and results were summarized as follows. 1. Plant height and shoot fresh weight of radish cultivars(long skirt, delicious altari, green mascot) were not different at the early growth(8 to 38 days after seeding), but increased at 55 days after seeding under foggy condition. Root fresh weight increased only in green mascot cultivar tinder foggy condition. Chlorophyll contents were higher in all the cultivars of radish under foggy condition. 2. Plant height and shoot fresh weight increased at 0 and 20 days of weed competition under foggy condition. Root fresh weight increased at 0, 20 and 35 days of competition under foggy condition. Above 50 days-competition, there was no difference between foggy and non-foggy condition. 3. Napropamide, alachlor and linuron did not show any differences in plant height and shoot fresh weight between foggy and non-foggy condition. Molinate reduced the root fresh weight at 65 days after seeding tinder foggy condition. 4. No difference in weeding efficacy by any herbicides tested was found tinder foggy condition. Weeding efficacy of Aeschynomene indica was reduced under foggy condition.

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