• Title/Summary/Keyword: RSD

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Determination of Allantoin in Dioscorea Rhizoma by High Performance Liquid Chromatography Using Cyano Columns

  • Yoon, Kee-Dong;Yang, Min-Hye;Chin, Young-Won;Park, Ju-Hyun;Kim, Jin-Woong
    • Natural Product Sciences
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    • v.14 no.4
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    • pp.254-259
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    • 2008
  • An easy and reliable HPLC method was developed to determine allantoin in Dioscorea Rhizoma using cyano columns. Qualitative and quantitative analyses of allantoin were performed successfully by cyano columns (YMC-Pack CN column, Zorbax SB-CN column and Discovery$^{(R)}$ Cyano column). The intraday precision were 0.58 - 3.33% for YMC-Pack CN, 0.41 - 2.20 for Zorbax SB-CN and 0.45 - 1.93% Discovery$^{(R)}$ Cyano columns, while interday variations were 0.09 - 1.84%, 0.04 - 2.59% and 0.87 - 5.18% for YMC-Pack CN, Zorbax SB-CN and Discovery$^{(R)}$ Cyano columns. The recoveries of allantoin were in the range at 98.8 - 102.6% (RSD 1.1 - 1.6%) for YMC-Pack CN column, 99.7 - 110.5% (RSD 1.3 - 4.9%) for Zorbax SB-CN column, and 97.2 - 110.1% (RSD 1.8 - 5.7%) for Discovery$^{(R)}$ Cyano column. The contents of allantoin in four Dioscorea Rhizoma samples were determined by cyano columms and ranged at 4.1-7.1 mg/g dry weight. The present study indicated that HPLC method using cyano column for determining allantoin is a reliable method and this method can be applied to verify allantoin in Dioscorea Rhizoma.

Rapid Identification of Radical Scavenging Compounds in Blueberry Extract by HPLC Coupled to an On-line ABTS Based Assay and HPLC-ESI/MS

  • Kim, Chul-Young;Lee, Hee-Ju;Lee, Eun-Ha;Jung, Sang-Hoon;Lee, Dong-Un;Kang, Suk-Woo;Hong, Sae-Jin;Um, Byung-Hun
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.846-849
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    • 2008
  • This study employed high performance liquid chromatography (HPLC) coupled to an on-line $ABTS^+$ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled $ABTS^+$-RSD system. The negative peaks of the $ABTS^+$-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-$ABTS^+$-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.

Validation of Method Determining Coixol in Coix lachryma-jobi var. ma-yuen Roots Extract (율무근 추출물의 Coixol 성분 분석법 검증)

  • Kwon, Jin Gwan;Seo, Changon;Choi, Yun-Hyeok;Choi, Chun Whan;Kim, Jin Kyu;Jeong, Wonsik;Lee, Ji Eun;O, Kyeong Hee;Hong, Seong Su
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.8
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    • pp.952-956
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    • 2017
  • An high performance liquid chromatography (HPLC) analysis method was developed for standard determination of coixol as a functional cosmetic material in Coix lachryma-jobi var. ma-yuen roots extract. HPLC was performed on a $C_{18}$ Unison US column ($4.6{\times}250mm$, $5{\mu}m$ column) using a gradient elution of 0.1% (v/v) trifluoroacetic acid and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 290 nm. The HPLC method was validated in accordance with the International Conference on Harmonization guideline of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limit of detection and quantitation were 0.07 and 0.25 mg/mL, respectively. Calibration curves showed good linearity ($R^2$>0.9995), and the precision of analysis was satisfied (less than 0.29%). Recoveries of quantified compounds ranged from 98.36 to 100.30%. This result indicates that the established HPLC method is very useful for the determination of a marker compound in C. lachryma-jobi var. ma-yuen roots extracts.

Changes in Rural Settlement Development System and its Effects on the facilities & houses of Village settlements in Rural area (농촌마을 개발제도의 변천과 농촌마을에 대한 영향)

  • Cho, Joon-Beom;Choi, Chan-Hwan
    • Journal of the Korean Institute of Rural Architecture
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    • v.1 no.2
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    • pp.109-117
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    • 1999
  • The purpose of the study is to evaluate the Rural Settlement Development(RSD) driven by government in historical view, and its effects on the facilities and houses of rural village in the country. Through the study, we have come to the results that the major concerns of the RSD projects since 1950s has been transferred to as following. First, the reformation of inter-facility; dressing room, kitchen, bath-house, etc of rural houses, from that of outer facility; roof, fence and etc. Secondly, the substantial objects of the projects of village settlement improvement have been changed from partial reformation of individual houses, public facilities, etc early, to comprehensive reformation by master plan for village settlement recently. Lastly, those have been established that the process of planning village settlement available to carry out landuse division and the various program for village improvement, based on its inherent circumstances.

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Simultaneous analysis method of BTEX and TPH in soil (토양중 BTEX와 TPH의 동시분석법에 관한 연구)

  • 신호상;박치후
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2000.05a
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    • pp.3-8
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    • 2000
  • A simple and rapid simultaneous analysis method of BTEX and TPH in soil was developed. 5g of soil sample were mixed with sodium sulfate and then extracted with 10 mL of mixture of acetone and dichloromethane (1:1). Extraction was performed for 10 min in sonicator and analysis was with GC-FID. The detection limits of BTEX and TPH was 0.8 and 10 mg/kg, respectively. The analytical recoveries were >90% for all BTEX and TPH. Low boiling point fuels and high boiling point fuels are consistently reproduced within RSD 7%. The analysis results show very simple and rapid quantitation of BTEX and TPH in soil sample with low RSD.

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Optical Purity Determination of (S)-Ibuprofen in Tablets by Achiral Gas Chromatography

  • Paik, Man-Jeong;Kim, Kyoung-Rae
    • Archives of Pharmacal Research
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    • v.27 no.8
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    • pp.820-824
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    • 2004
  • An optical purity test was indirectly performed on (S)-ibuprofen as its diastereomeric (R)-(+)-1-phenylethylamide derivative using achiral gas chromatography (GC). The method for the determination of trace (R)-ibuprofen (optical impurity), within the range 1.0 to 50 ng, from a racemic ibuprofen standard was linear (r=0.9997) with acceptable precision (% $RSD{\leq}5.3$) and accuracy (% RE=0.7~-3.9). Similar results were obtained with the method validation for the quantification of (S)-ibuprofen within the range 0.1 to 2.0 $\mu\textrm{g}$ using a (S)-ibuprofen stan-dard. When applied to seven different commercial (S)-ibuprofen products, their optical purities (98.7~99.1%) were determined with good precision (% $RSD{\leq}4.0$).

Simultaneous Determination of Cinnamaldehyde and Coumarin in Oryeong-san using HPLC with Photodiode Array Detector

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Herbal Formula Science
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    • v.18 no.2
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    • pp.251-257
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    • 2010
  • Objectives : To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was $H_2O$ and solvent B was acetonitrile. Results : Calibration curves were acquired with correlation coefficient ($r^2$)>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g. Conclusions : The established HPLC method will be helpful to improve quality control of ORS.

Application of ANOVA and DOE by Using Randomized Orders and Geometrical Properties (랜덤화 순서와 기하학적 특성을 고려한 분산분석과 실험계획의 응용방안)

  • Choe, Seong-Un
    • Proceedings of the Safety Management and Science Conference
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    • 2012.04a
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    • pp.277-292
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    • 2012
  • The research presents an application of Balanced ANOVA (BANOVA) by utilizing randomized orders for various Split-Plot Designs (SPDs) which include two cell designs, split-plot with one-way HTC (Hard to Control) factor, split-plot with two-way HTC factor, split-split-plot design and nested design. In addition, four MINITAB examples of 2-level split-plot designs based on the number of blocks and the type of whole-plots are presented for practitioners to obtain comprehensive understanding. Furthermore, the geometrical interrelated properties among three typical Designs of Experiments (DOE), such as Factorial Design (FD), Response Surface Design (RSD), and Mixture Design (MD) are discussed in this paper.

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Quantification of Globotriaosylsphingosine in Urine using UPLC-ESI-MS/MS; Application for Screening Fabry Disease (파브리병의 신속한 진단을 위한 소변 중 Globotriaosylsphingosine의 UPLC-ESI-MS/MS 분석법)

  • Yoon, Hye-Ran
    • YAKHAK HOEJI
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    • v.60 no.1
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    • pp.15-20
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    • 2016
  • Globotriaosylsphingosine (lyso Gb3) is considered as one of the biomarkers for Fabry disease. A rapid and simple UPLC-MS/MS method was developed for the determination of reliable biomarker, lyso Gb3. Total analytical procedure takes only 15 min including sample preparation and MS/MS analysis. Limit of detection was 0.85 ng/ml (S/N=3). The calibration curve was linear over the range of 2.0~400.0 ng/ml ($R^2=0.9999$). Inter-day and intra-day assay accuracy were 93.4~100.6% (RSD, 0.6~6.0%) and 97.5~100.7% (RSD, 3.6~5.2%). Absolute recoveries of 97.6~98.6 showed excellence of a new analytical method. The method was applied to human and mice urines, proved the suitability for the quantification of lyso-Gb3 for screening, diagnosis and therapeutic monitoring of Fabry disease patients.

Pair-wise peculiar velocity and the redshift space distortion

  • Park, Hyunbae;Tonegawa, Motonari;Zheng, Yi;Sabiu, Cris;Li, Xiao-dong;Park, Changbom
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.2
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    • pp.43.2-43.2
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    • 2018
  • The line-of-sight component in the relative motion of galaxy pairs sources the redshift space distortion (RSD) in galaxy surveys. By knowing the probability density function (PDF) of pair-wise motions and projecting it to the line-of-sight direction, one can compute the RSD effect precisely. I present the pair-velocity PDF of dark matter and galaxies in the Horizon-run 4 simulation. I also derive a model motivated by the perturbation theory which fits the results fairly well. I also discuss the application of the model in constraining the cosmology.

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