• Genomics & Informatics
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• 제8권4호
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• pp.194-200
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• 2010

The PIK3CA gene, oncogenic gene located on human chromosome 3q26.3, is an important regulator of cell proliferation, death, motility and invasion. To evaluate the role of PIK3CA gene in the risk of Korean lung cancer, genotypes of the PIK3CA polymorphisms (rs11709323, rs2699895, rs3729679, rs17849074 and rs1356413) were determined in 423 lung cancer patients and 443 normal controls. Statistical analyses revealed that the genotypes and haplotypes in the PIK3CA gene were not significantly associated with the risk of lung cancer in the Korean population, suggesting that these PIK3CA polymorphisms do not contribute to the genetic susceptibility to lung cancer in the Korean population.

• Clinical and Experimental Pediatrics
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• 제53권4호
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• pp.548-553
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• 2010

목 적: IgA 신병증은 소아들의 만성 사구체 신염 중에서 가장 흔하게 일어나며, HLA유전자는 다양한 염증성 질환과 자가면역질환과 연관이 있어 왔다. 이 연구에서는 한국인에서 건강한 대조군과 IgA 신병증 환자군을 비교하여 IgA 신병증 발생 감수성 및 병리, 임상 양상과 $HLA-G$ 유전자의 SNP와의 연관성에 관해 알아보고자 하였다. 방 법: 소아 IgA 신병증을 앓고 있는 174명의 환자군과 438명의 정상 대조군에서 $HLA-G$ 유전자의 promoter SNP (rs1736936과 rs2735022)를 분석하고 비교하였다. 또한 IgA 신병증 환자들을 단백뇨($4mg/m^2/hour$ 이하군과 이상군)의 유무, 족세포 돌기의 소실 유무, 간질의 섬유화 및 세뇨관 위축이나 미만성 사구체 경화와 같은 병리학적 소견상 진행성 질환의 표지자유무에 따라 하위그룹으로 나누어 비교하였다. 결 과: IgA 신병증 환자군과 정상 대조군 간의 HLA-G에서의 SNP (rs1736936과 rs2735022) 빈도에 대한 유의한 차이는 발견되지 않았다. 또한 단백뇨의 유무, 족세포 돌기의 소실 유무, 질환의 병리학적 진행 정도을 의미하는 표지자의 유무와 SNP사이에서도 유의한 연관성을 보이지는 않았다. 그러나, 일체 배형으로서 rs1736936과 rs2735022는 소아 IgA 신병증을 일으키는 감수성에 대해 통계학적으로 유의한 연관성을 나타내었다(haplotype T/C: dominant model OR 1.71, 95% CI 1.00-2.92, $P$=0.049; haplotype C/T: recessive model OR 0.54, 95% CI 0.31-0.94, $P$=0.030). 결 론: 이번 연구에서 $HLA-G$ 유전자의 SNP 중 rs1736936와 rs2735022로 이루어진 일체배형과 IgA 신병증의 발생간에 유의한 관계를 관찰하였으며, IgA 신병증 환자들의 단백뇨 발생 유무, 족세포 돌기의 소실 유무 및 질병 진행 정도로 구분된 하위그룹과 후보 SNP들간의 유의한 관계는 확인할 수 없었다.

• 분석과학
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• 제35권4호
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• pp.161-168
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• 2022

Wheat is rich in sources of fiber, oligosaccharides, and resistant starch, simple carbohydrates which may have a protective role against carcinoma. Additionally, Whole wheat/bran as well includes contains phytochemicals such as flavonoids, lignans, folate, phytosterols, phenolic acids, and tocols. The above phytochemicals suitable forms antioxidant and cholesterol-reducing activities. Phytoestrogens are regarded as especially essential in the preventative measures of hormonally dependent malignancies such as breast cancer (BC). In this study lowered BC risk has been associated with whole grain/bran consumption with an odds ratio (OR=0.24 and 95 %CI=0.10-0.56). Wheat/bran appears to have a reliable protective impact against BC. While intake of white bread has been associated with a high risk of BC (OR=2.63 and CI 95 %=1.07-6.48). Also, the E-Selectin (SELE) Single nucleotide polymorphisms (SNPs) rs5353 A/G and rs932307 C/T were investigated using the sanger sequences approach. There was a positive association between genotypes (rs5353 GG+AG) and (rs932307 TT+CT) with rate consumption of wheat-white/bread and these genotypes were more frequent in patients had BC in comparison with a significant difference (P=0.03) (P=0.01), respectively. The genotypes (GG+AG) frequency of the rs5353 polymorphism and (TT+CT) genotypes of the rs932307 polymorphism in the present study had a high risk of cancer with (OR=3.05), (OR=4.17) respectively. While these genotypes showed no significant association with the rate of whole grain consumption in patients and control. Therefore, the type of wheat may associate with increased incidence of disease-related with type of Polymorphism because some present genotypes of SNPs showed high (OR) which may refer to their positive associated with disease, the white wheat consumption may active the risky association between SNPs and BC.

• 한국약용작물학회지
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• 제9권4호
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• pp.284-289
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• 2001

한약재로 이용되고 있는 육묘한 식방풍을 공시하여 육묘이식 백색비닐피복, 육묘이식 흑색비닐피복, 육표이식 무피복 처리와 노지직파 무피복처리가 생육과 수량에 미치는 영향에 관하여 시험을 수행한 결과는 다음과 같다. 출아기간은 폿트육묘시 8일이었고 노지직파는 40일이었으며 출아율은 폿트육묘시 74%였으나 노지직파에서는 17%로 크게 저조하였다. 초장, 엽수, 경직경 등 지상부생육량은 육묘이식 백색비닐피복>육묘이식 흑색비닐피복 >육묘이식 무피복>노지직파 무피복처리구 순으로 많은 경향이었다. 주근의 길이는 육묘이식 무피복구가 유의하게 짧았으나, 피복처리구와 노지직파 무피복구는 비슷하게 긴편이었다. 주근의 굵기는 비닐피복구가 무피복구에 비하여 유의하게 굵었고 노지직파 무피복구가 가장 가늘었다. 지근의 수는 육묘이식한 것이 직파한 것보다 $4.7{\sim}7.1$개 많았다 건근비율은 노지직파 무피복구에서 30.5%로 가장 높고 육묘이식구에서는 무피복구가 높았으머 비닐피복구에서 낮은 경향이었다. 10a당 건근수량은 육묘이식 백색비닐피복 542kg, 육묘이식 흑색비닐피복 506kg, 육묘이식 무피복 419kg으로 노지직파 7kg에 비하여 각각 52, 42, 17%증가하였으며 식방풍의 재배는 육묘이식하되 백색비닐로 피복하여 재배하는 것이 가장 유리하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8405-8410
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• 2016

Hepatitis B virus (HBV) infection is the leading cause of hepatocellular carcinoma (HCC) development. Recent studies demonstrated that single nucleotide polymorphisms (SNPs) rs2293152 in signal transducer and activator of transcription 3 (STAT3) and rs7574865 in signal transducer and activator of transcription 4 (STAT4) are associated with chronic hepatitis B (CHB)-related HCC in the Chinese population. We hypothesized that these polymorphisms might be related to HCC susceptibility in Thai population as well. Study subjects were divided into 3 groups consisting of CHB-related HCC (n=192), CHB without HCC (n=200) and healthy controls (n=190). The studied SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that the distribution of different genotypes for both polymorphisms were in Hardy-Weinberg equilibrium (P>0.05). Our data demonstrated positive association of rs7574865 with HCC risk when compared to healthy controls under an additive model (GG versus TT: odds ratio (OR)=2.07, 95% confidence interval (CI)=1.06-4.03, P=0.033). This correlation remained significant under allelic and recessive models (OR=1.46, 95% CI=1.09-1.96, P=0.012 and OR=1.71, 95% CI=1.13-2.59, P=0.011, respectively). However, no significant association between rs2293152 and HCC development was observed. These data suggest that SNP rs7574865 in STAT4 might contribute to progression to HCC in the Thai population.

• 한국지리정보학회지
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• 제3권4호
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• pp.63-72
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• 2000

본 연구의 목적은 GIS와 RS(Landsat TM, KOMPSAT EOC) 데이터를 이용한 AGNPS(Agricultural Nonpoint Source Pollution) 모형의 적용성을 판단하기 위한 것이다. AGNPS 모형은 잘 알려진 분포형 강우사상 모형으로써 이 모형은 밭의 시비수준과 축산농가의 오염 부하 수준을 적용할 수 있어 이를 효율적으로 관리할 수 있는 방법을 평가 할 수 있다. 본 연구의 대상 유역은 경기도 안성군 고산면(정동리, 야동리, 쌍령리)에 위치하고, 20개의 소규모 축산 농가가 산재해 있는 면적 $4.12km^2$ 내에 소유역이다. AGNPS 모형의 입력 데이터는 Arc/Info, GRASS, ER-Mapper, Idrisi를 사용하여 구축되었다. 모형의 보정 및 검증을 위해 사용된 강우 자료는 1시간 간격으로 관측된 1999년의 4개의 강우사상(5월 18일, 7월 29일, 9월 10일, 9월 20일)을 사용하였고, 수질 측정은 4개의 지점에서 측정된 실측자료를 사용하였다.

• 한국식품과학회지
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• 제29권3호
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• pp.516-521
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• 1997

효소저항전분의 물리적 성질에 대한 아밀로오스 함량의 효과를 알아보기 위해 아밀로오스 함량이 다른 옥수수 전분인 Amioca, PFP, Amaizo, Amylomaize VII을 사용하여(전분 : 물=1 : 3.5) $121^{\circ}C$로 가열-냉각 싸이클 4회까지 반복하고 가열-냉각된 옥수수 전분 (AMS)과 분리된 효소저항전분(RS)의 흡수력, 형태학적 특성, 열적 특성 등의 물리적 특성을 비교하였다. Amioca, PFP, Amaizo, Amylomaize VII의 아밀로오스 함량은 각각 0%, 29%, 50%, 72% 였으며, 아밀로오스 함량이 많을수록 RS 수율이 높았고, 흡수력은 RS<생전분$155^{\circ}C$ 근처에서 흡열피크를 나타낸 반면, Amioca와 PFP는 아밀로펙틴의 용융피크인 $40{\sim}70^{\circ}C$에서 피크를 나타내었으며, Amioca를 제외한 모든 RS는 $155^{\circ}C$ 근처에서 흡열피크를 나타내었으나 엔탈피는 아밀로오스 함량과 상관이 적었다.

• Journal of Microbiology
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• 제35권4호
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• pp.277-283
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• 1997

Promoters inducible by paraquat, a superocide-generating agent, were isolated from Escherichia coli using a promoter-probing plasmid pRS415 with promoterless lacA gene. Twenty one promoters induced by paraquat were selected and further characterized. From sequence analysis, thirteen of the promoters were mapped to their specific loci on the Escherichia coli chromosome. Several promoters were mapped to the upstream of known genes such as usgl, katG, and mglB, whose relationships with superoxide response have not been previously reported. Other promoters were mapped to the upstream region of unknown open reading frames. Downstream of HC 96 promoter are uncharacterized ORFs whose sequences are homologous to ABC-transporter subunits. Downstream of HC84 promoter is an ORF encoding a transcriptional regulator-like protein, which contains a LysR family-specific HTH (helix-turn-helix) DNA bindign motif. We investigated whether these promoters belong to the soxRS regulon. All promoters except HC96 were found to belong to the soxRS regulon. The HC96 promoter was significantly induced by paraquat in the soxRS deletion mutant strain. The basal transcription level of three promoters (HE43, HC71, HD94) significantly increased at the stationary phase, implying that they are regulated by RpoS. However, paraquat inducibility of all promoters disappeared in the stationary phase, suggesting that SoxRS regulatory system is active only in rapidly growing cells.

• The Korean Journal of Physiology and Pharmacology
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• 제7권6호
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• pp.303-306
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• 2003

Metabotropic glutamate receptors (mGluRs), classified into three groups (group I, II, III), play a critical role in modulation of synaptic transmission at central and peripheral synapses. In the present study, extracellular field potential recording techniques were used to investigate effects of mGluR agonists on excitatory synaptic transmission at thalamic input synapses onto the lateral amygdala. The non-selective mGluR agonist t-ACPD ($100{\mu}M$) produced reversible, short-term depression, but the group III mGluR agonist L-AP4 ($50{\mu}M$) did not have any significant effects on amygdala synaptic transmission, suggesting that group I and/or II mGluRs are involved in the modulation by t-ACPD. The group I mGluR agonist DHPG ($100{\mu}M$) produced reversible inhibition as did t-ACPD. Unexpectedly, the group II mGluR agonist LCCG-1 ($10{\mu}M$) induced long-term as well as short-term depression. Thus, our data suggest that activation of group I or II mGluRs produces short-term, reversible depression of excitatory synaptic transmission at thalamic input synapses onto the lateral amygdala. Considering the long-term effect upon activation of group II mGluRs, lack of long-term effects upon activation of group I and II mGluRs may indicate a possible cross-talk among different groups of mGluRs.

• 대한의생명과학회지
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• 제27권3호
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• pp.187-194
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• 2021

Platelets are derived from fragments formed in the cytoplasm of bone marrow megakaryocytes. Platelet count (PLT) can be altered by factors such as platelet production, destruction, and inflammation. In a previous study, the significant single nucleotide polymorphisms (SNP) were reported by the genome-wide association study (GWAS) for PLT in Koreans. In this study, it was confirmed whether significant SNPs were replicated in the HEXA (The Health Examinees) cohort. As a result, the SNPs of the THPO (rs6141), BAK1 (rs210314, rs9296095), GGNBP1 (rs75080135), ACAD10 (rs6490294), and ABCC4 (rs4148441) were significantly correlated with PLT (P < 10^-8). At the same time, it was confirmed that the direction of influence was the same according to the genotype. In conclusion, it can be seen that common SNPs are associated with the platelet count regardless of the cohort for Koreans.