• The Plant Pathology Journal
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• v.30 no.3
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• pp.254-260
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• 2014

Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBSLRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.3
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• pp.371-381
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• 2023

Evaluating the effectiveness of the radiation protection measures deployed at the Centralized Radioactive Waste Management Facility in Ghana is pivotal to guaranteeing the safety of personnel, public and the environment, thus the need for this study. Radiagem^TM 2000 was used in measuring the dose rate of the facility whilst the personal radiation exposure of the personnel from 2011 to 2022 was measured from the thermoluminescent dosimeter badges using Harshaw 6600 Plus Automated TLD Reader. The decay store containing scrap metals from dismantled disused sealed radioactive sources (DSRS), and low-level wastes measured the highest dose rate of 1.06 ± 0.92 µSv·h^-1. The range of the mean annual average personnel dose equivalent is 0.41-2.07 mSv. The annual effective doses are below the ICRP limit of 20 mSv. From the multivariate principal component analysis biplot, all the personal dose equivalent formed a cluster, and the cluster is mostly influenced by the radiological data from the outer wall surface of the facility where no DSRS are stored. The personal dose equivalents are not primarily due to the radiation exposures of staff during operations with DSRS at the facility but can be attributed to environmental radiation, thus the current radiation protection measures at the Facility can be deemed as effective.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.418-424
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• 2018

${\text\tiny{D}}-Allose$ is a potential medical sugar because it has anticancer, antihypertensive, antiinflammatory, antioxidative, and immunosuppressant activities. Allose production from fructose as a cheap substrate was performed by a one-pot reaction using Flavonifractor plautii ${\text\tiny{D}}-allulose$ 3-epimerase (FP-DAE) and Clostridium thermocellum ribose 5-phosphate isomerase (CT-RPI). The optimal reaction conditions for allose production were pH 7.5, $60^{\circ}C$, 0.1 g/l FP-DAE, 12 g/l CT-RPI, and 600 g/l fructose in the presence of 1 mM $Co^{2+}$. Under these optimized conditions, FP-DAE and CT-RPI produced 79 g/l allose for 2 h, with a conversion yield of 13%. This is the first biotransformation of fructose to allose by a two-enzyme system. The production of allose by a one-pot reaction using FP-DAE and CT-RPI was 1.3-fold higher than that by a two-step reaction using the two enzymes.

• Journal of Dental Rehabilitation and Applied Science
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• v.30 no.3
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• pp.259-264
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• 2014

Retrograde peri-implantitis (RPI) is defined as a clinically symptomatic periapical lesion. RPI is generally accompanied by symptoms of pain, tenderness, swelling and fistula. Several etiologic factors of RPI were possible, residual bacteria would be the main cause of RPI. Various treatment modalities have been introduced: debridement only or a combination of debridement with the grafting material accompanied by a detoxification of the infected implant surfaces, apicoectomy and so on. Although the definitive management methods remain undefined, many favorable clinical results of a treatment of RPI have been published. This case report introduces the 7-year long-term clinical result of the application the principle: implant surface detoxification using saline and chlorhexidine and guided bone regeneration with bone graft material and barrier membrane. If the implant was not mobile, it would be possible to treat RPI according to surgical approach and good results will be maintained over long term.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.3 no.2
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• pp.194-203
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• 1993

Quality Control programs for industrial hygiene laboratory have successfully been operated since the 1st round was initiated in April 1992. Three rounds have been completed. Overall analytical performance of participants was improved through a subsquent round. The first round had a large variation among analytical results of all participants, 26.34%~504.22% of coefficient of variation. But the variation of analytical performance in the 2nd and 3rd rounds decreased to 40.42%~52.55% and 10.74%~20.98%, respectively. The difference of analytical performance among participants was decreased by operation of subsquent round. By distribution of Running Performance Index (RPI), over 50% of participants belongs to Category 3 in the first round. The average percentage of participants assigned to Category 3 in the 2nd and 3rd round was decreased to 37.2%. A definite analytical performance improvement of participants seems to be obtained. "A" and "B" group have more participants assigned to Category 3 than those of "C" group through the distribution of RPI by group divided to similar participant. By the distribution of RPI all participants, the percent of participant being over 200 RPI in the 1st round was 43%~52%, but decreased to 9%~29% and 9%~27%, in the 2nd and 3rd round, respectively. These results mentioned above indicate that the analytical performance of all participants have been improved by subsquent Quality Control Program although newcomers joined and a few participants droped out. But, there are some participants with poor analytical performance. Industrial Health Research Institute (IHRI) will offer service such as education, communication and visitation to them, and improve quality of their analytical performance in the future.

• Genomics & Informatics
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• v.9 no.1
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• pp.39-43
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• 2011

MicroRNAs (miRNAs) are recently discovered small RNA molecules usually resulting in translational repression and gene silencing. Despite the fact that specific cloning of small RNA's is a method in practice, computational identification of miRNA's has been a major focus recent days, since is a rapid process following AB initio and sequence alignment methods. Here we developed new software called MiRPI that aims to identify the highly conserved miRNAs without any mismatches from given fasta formatted gene sequences by using non-repeated miRNA dataset of the user's interest. The new window embedded with the software is used to identify the targets for inputted mature miRNAs in the mRNA sequences. Also MiRPI is designed to measure the precursor miRNA statistics, majorly focusing the Adjusted Minimum Folding free Energy (AMFE) and Minimum Folding free Energy Index (MFEI), the most important parameters in miRNA confirmation. MiRPI is developed by PERL (Practical Extraction and Report Language) and Tk (Tool kit widgets) scripting languages. It is user friendly, portable offline software that works in all windows OS, sized to 3 MB.

• Journal of Technologic Dentistry
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• v.34 no.4
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• pp.473-482
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• 2012

Purpose: The purpose of this study was to investigate the stress distribution of the surrounding tissues according to 3 proximal plate types of the RPI clasp. Methods: The removeable partial denture which mandibular right and left second premolars and mandibular molars were lost was attached to a three dimensional photo elastic epoxy resin model. Then 120N of vertical load was applied. After 3-dimensional photoelastic stress analysis was used to record the isochromatic fringe patterns. Results: Kratochvil type guiding plane exhibited little uniform stress distribution on load center and alveolar ridge, but higher stress concentration on buccal surface of second premolar. Krol type guiding plane exhibited the stress concentration on the front of load center and relatively higher stress concentration on buccal surface of first premolar. However, this type had no effect on canine. Researcher type guiding plane showed the stress concentration on second premolar and molar, but the little stress distribution on first premolar. Conclusion: In all types, excessive stress concentration was appeared and three types were not significant different.

• Journal of Korean Home Economics Education Association
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• v.24 no.2
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• pp.51-62
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• 2012

This research examined the method and amount changes of recommended protein intakes(RPI) for male and female adult, and pregnant lactating women from 1962's Recommended Dietary Allowances for Korean(KRDA) to 2010's Dietary Reference Intakes for Koreans(KDRIs) revised. As male and female adult's RPI calculation, factorial method was applied until 1989 KRDA, after that nitrogen balance study was applied. Basal factor in factorial method was standard protein(egg or milk protein) requirement or obligatory nitrogen(protein) loss. On the other hand, basal factor in nitrogen balance study was minimum dietary protein requirement to maintain nitrogen equilibrium balance(nitrogen intake = nitrogen excretion). Adjusting factors of RPI were stress and/or protein requirement difference among people. The RPI of male adults were 50~80 g/day, that of female adults were 45~70 g/day. The additional RPI of pregnant women were 10~30 g/day, were calculate based upon the extra protein needs caused by unborn child development. The pregnant women's additional RPI of 2010's KDRIs revised in the periods of first, second, and third trimester were 0, 15, 30 g/day, respectively. The additional RPI of lactation women were 20~30 g/day, were calculated based upon the extra protein needs caused by maternal milk secretion.

• Korean Journal of Food Science and Technology
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• v.12 no.2
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• pp.109-114
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• 1980

Protein efficiency ratio (PER), net protein utilization (NPU), nitrogen digestibility (ND), organ weights, and amino acid composition were investigated for rapeseed protein isolates (RPI) prepared by alkaline countercurrent extraction and isoelectric precipitation of defatted rapeseed meal (DRM). The PER values for 3 kinds of RPI obtained at pI of 6.7, 5.6 and 5.0 were 2.8, 3.1 and 2.9, and for DRM 2.5 while the NPU values for these RPI appeared to be 68, 73 and 71 %, and for DRM 56 %, respectively. Mean ND (85%), food intake (87), and weight gain (37.6) for RPI were significantly different from those of DRM (71 %, 77, and 28, respectively). There was no signicant difference in the PER among three RPI and casein (3.0), nor in the NPU among those proteins and casein (74%). These data attributed to the favorable amino acid patterns of proteins isolated which contained balanced essential amino acids in proportions which meet the requirements of human adult(FAO/WHO, 1973).

• The Journal of Korean Academy of Prosthodontics
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• v.30 no.2
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• pp.203-224
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• 1992

The purpose of this study was to evaluate the stress distribution developed in supporting structures by distal extension removable partial denture with 4-types of direct retainer. The direct retainers examined were Akers clasp, RPI clasp, RPA clasp and RPL clasp in bilateral & unilateral free end case. 3-dimensional photoelastic stress analysis was used to record the isochromatic fringe patterns and to calculate the compressive stress at measuring points. The results were as follows. 1. In bilateral free end case, RPI clasp exhibited the similar stress distribution on distal and mesial alveolar crest but Akers clasp exhibited higher stress concentration on distal alveolar crest than mesial alveolar crest. 2. In bilateral free end case, RPA clasp and RPL clasp exhibited the similar stress distribution on distal and mesial alveolar crest and RPL clasp exhibited higher stress concentration on buccal alveolar crest than lingual alveolar crest. 3. Akers clasp produced high stress concentration on residual alveolar ridge distally, but RPI clasp, RPA clasp and RPL clasp produced even stress distribution on residual alveolar ridge. 4. Removable partial denture in unilateral free end case exerted higher stress on abutment tooth root apex than bilateral distal extension removable partial denture.