• 한국동물생명공학회지
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• 제34권4호
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• 한국정보통신학회논문지
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• 제23권12호
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• pp.1679-1686
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• 2019

본 논문에서는 RPL을 사용하는 사물인터넷 환경에서 노드들이 이동할 때에도 토폴로지 변경에 빠르게 적응하여 패킷 손실 문제를 해결하기 위한 방안을 제안한다. 이동성을 향상시키기 위해 모든 노드는 이웃 노드들의 이동성을 인식하고, 전체 수신 패킷과 제어메시지 수를 고려하여 이동 정도를 수치화한다. 이동 정도에 따라 DIO 타이머를 동적으로 설정하여 토폴로지 변경을 빠르게 인식하고 목적지까지의 경로를 업데이트할 수 있도록 한다. 제안 방식의 성능은 Contiki 기반 Cooja 시뮬레이터를 이용하여 다양한 이동 속도에 대해서 평가한다. 시뮬레이션 결과, 제안된 방식은 패킷 전달률이 31.03% 개선됨을 확인하여 표준 RPL보다 이동성 시나리오에 잘 대처함을 보여준다.

• 정보과학회 논문지
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• 제42권10호
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• pp.1268-1279
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• 2015

사물인터넷(IoT)에 연결된 다수의 장치를 관리하기 위해서 IETF는 IPv6를 지원하는 RPL 라우팅 프로토콜을 제안하였다. RPL은 사물인터넷이 구축되는 네트워크에서 요구되는 서비스에 맞는 OF(Objective Function)를 통해 경로가 생성된다. RPL에서는 단순하게 노드 간 링크 품질만을 고려하여 경로를 구축하므로, 다양한 목적의 QoS를 제공할 수 있는 OF에 대한 연구가 필요하다. 기존의 연구에서는 주로 에너지 소비 감소를 다루고 있고, 싱크 노드까지 형성된 경로의 링크 품질을 고려하지 않고 있어 종단 간 지연시간이 늘어 날 수 있다. 본 논문에서는 트래픽이 몰려 병목현상이 일어나는 노드의 예상 수명과 목적지까지 형성될 전체 경로의 품질을 고려하여 지연시간을 줄이는 기법을 제안한다. 실험을 통해 제안 기법의 가중치를 설정하였고, 종단 간 지연시간을 약 20.8%, 에너지 소비를 약 10.5% 개선하였음을 보였다.

• 실천공학교육논문지
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• 제9권1호
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• pp.57-68
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• 2017

본 연구에서는 코리아텍 사례를 바탕으로 대학형 일학습병행제에 선행경험학습인정을 도입하기 위한 실질적인 준비 과정과 그 결과물을 분석하고 시사점을 도출하고자 하였다. 또한 선행경험학습인정과 관련된 국내외 선행 연구 및 사례들을 종합적으로 정리함으로써 국내 다른 교육기관들이 선행경험학습인정을 도입하고자 할 때 고려해야 할 사항들에 대한 이해를 높이고자 하였다. 본 사례 연구 결과를 바탕으로 보다 많은 교육기관들의 성공적인 선행경험학습인정 정착을 위해 필요한 제도적 지원, 문화적 지원, 대학 내부의 지원 방안을 제안하였다.

• 한국멀티미디어학회논문지
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• 제21권9호
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• pp.1090-1098
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• 2018

MQTT is a lightweight messaging protocol that can be used for low power IoT devices. The MQTT basically uses single MQTT broker to indirectly share message information between publishers and subscribers. This approach has a weakness in regard to traffic overflow, connection fault, security, etc. In this paper, we propose a distributed MQTT broker architecture that solves the problems in single MQTT broker structure. The distributed MQTT broker architecture is expected to support new application services that cannot be supported by a conventional MQTT architecture. We have designed and implemented a distributed MQTT broker architecture based on the RPL protocol that has been widely used for IoT network. The experiment results show that the proposed MQTT broker architecture represents better publishing/subscribing latency and network stability than the conventional MQTT broker architecture.

• 한국정보통신학회논문지
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• 제22권1호
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• pp.196-201
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• 2018

최근 로컬 IoT 네트워크 구축과 관련하여 RPL 프로토콜을 활용하는 다양한 비-인프라 기반 IoT 네트워킹 기법들이 연구 중이다. 특히, RPL 프로토콜은 자율네트워킹과 로컬 네트워크에 존재하는 노드 간 애드혹 경로를 제공하지 못하는 문제를 가지고 있다. 본 논문에서는 비-인프라 기반 IoT 네트워크 구축을 지원하는 자율네트워킹 기법을 제안한다. 제안하는 기법은 저전력 손실 네트워크로 구성된 네트워크 환경에 적용 가능한 자율네트워킹 기법이다. 실험을 통해서 제안한 자율네트워킹 기법의 우수한 성능을 보였다. 특히, 단대단 데이터 발생률과 단대단 지연 측면에서 제안하는 기법의 성능이 우수함을 증명하였다.

• 한국약용작물학회지
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• 제18권3호
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• pp.173-179
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• 2010

This study describes an efficient approach to the development of DNA markers for use in distinguishing the Scrophularia species that have been used as useful medicinal crops. In order to distinguish Scrophularia species, DNA sequences of rpl-5 region in mitochondrial DNA of Scrophularia species were analysed for detecting sequence variations, and the PCR-RFLP method was applied for developing practicable DNA marker patterns. Several DNA variations were detected by the sequence comparison of rpl-5 region among Scrophularia species. Genetic relationship analysis of Scrophularia species was carried out based on these DNA variations. DNA variations of rpl-5 region were revealed that it was significantly efficient in genetic relationship analysis of Scrophularia species. In addition, Scrophularia species tested in this study were completely discriminated by four polymorphic genotypes by PCR-RFLP combined with Tsp509 I (^AATT) restriction enzyme. Our results suggested that DNA sequence variations of rpl-5 region were sufficiently useful for genetic relationship analysis of Scrophularia species. Polymorphic genotypes by PCR-RFLP using the Tsp509 I enzyme will be useful for discrimination of Scrophularia species as a practicable DNA markers.

• 한국인터넷방송통신학회논문지
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• 제19권5호
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• pp.123-128
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• 2019

IETF(Internet Engineering Task Force)는 LLN(Low power and Lossy Networks)의 라우팅 프로토콜로 RPL(IPv6 Routing Protocol for Low-power Lossy Network)을 제안하였다. RPL을 사용하는 네트워크에서는 하나의 parent 노드가 많은 child 노드와 연결되어, 일부 노드로 연결이 몰리는 Thundering Herd Phenomenon이 발생할 수 있다. 이를 해결하기 위해 CNC(Child Number Count)를 사용하여 노드 당 연결되는 최대 child 노드 수를 제한하는 방법이 고려되었으나 여전히 일부 parent 노드에 최대 child 노드 수 만큼 붙을 수 있다는 문제와 최대 child 노드 수를 몇으로 결정하는가 하는 문제가 남아있다. 따라서 본 논문에서는 노드 당 연결되는 child 노드 수를 고르게 분배하는 알고리즘을 제안하여, Thundering Herd Phenomenon 문제를 해결하고자 한다. 기존 CNC를 사용하는 알고리즘과 성능 비교를 진행하였고, 그 결과 제안하는 알고리즘이 load balancing 측면에서 더 좋은 성능을 보이는 것을 검증하였다.

• 대한방사선기술학회지:방사선기술과학
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• 제46권3호
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• pp.219-229
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• 2023

In this study, basic characteristics such as reproducibility, linearity, and directionality of RPL glass dosimeters were evaluated to improve the reliability of dose evaluation through RPL glass dosimeters, and uncertainty elements such as sensitivity by glass element and magazine slot sensitivity were evaluated. Using a mathematical model to calibrate the measured values of the RPL glass dosimeter, the measurement uncertainty was calculated assuming an example. As a result of the characteristic evaluation, the RPL glass dosimeter showed excellent performance with a standard deviation of ±1% (1 SD) for the reproducibility of the reading process, a coefficient of determination for linearity of 0.99997. And the read-out of the RPL glass dosimeter are affected by the circular rotation direction of the glass dosimeter during irradiation, fading according to the period after irradiation, the number of laser pulses of the reader, and response degradation due to repeated reading, it is judged that measurement uncertainty can be reduced by irradiation and reading in consideration of these factors. In addition, it was confirmed that the dose should be determined by calculating the correction factors for the sensitivity of each element and, the sensitivity of each reading magazine slot. It is believed that the reliability of dosimetry using glass dosimeters can be improved by using a mathematical model for correction of glass dosimeter readings and calculating measurement uncertainty.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.