• ALGAE
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• 제17권1호
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• pp.59-68
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• 2002

A phylogenetic affinity between charophytes and embryophytes (land plants) has been explained by a few chloroplast genomic characters including gene and intron (Manhart and Palmer 1990; Baldauf et al. 1990; Lew and Manhart 1993). Here we show that a charophyte, Spirogyra maxima, has the largest operon of angiosperm chloroplast genomes, rpl23 operon (trnⅠ-rpl23-rpl2-rps19-rpl22-rps3-rpl16-rpl14-rps8-infA-rpl36-rps11-rpoA) containing both embryophyte introns, rpl16.i and rpl2.i. The rpl23 gene cluster of Spirogyra contains a distinct eubacterial promoter sequence upstream of rpl23, which is the first gene of the green algal rpl23 gene cluster. This sequence is completely absent in angiosperms but is present in non-flowering plants. The results imply that, in the rpl23 gene cluster, early charophytes had at least two promoters, one upstream of trnⅠ and and another upstream of rpl23, which partially or completely lost its function in land plants. A comparison of gene clusters of prokaryotes, algal chloroplast DNAs and land plant cpDNAs indicated a loss of numerous genes in chlorophyll a+b eukaryotes. A phylogenetic analysis using presence/absence of genes and introns as characters produced trees with a strongly supported clade containing chlorophyll a+b eukaryotes. Spirogyra and embryophytes formed a clade characterized by the loss of rpl5 and rps9 and the gain of trnⅠ (CAU) and introns in rpl2 and rpl16. The analyses support the hypothesis that the rpl23 gene cluster and the rpl2 and rpl16 introns of land plants originated from a common ancestor of Spirogyra and land plants.

• Molecules and Cells
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• 제27권5호
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• pp.539-546
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• 2009

In Saccharomyces cerevisiae, ribosomal protein L7, one of the ~46 ribosomal proteins of the 60S subunit, is encoded by paralogous RPL7A and RPL7B genes. The amino acid sequence identity between RPl7a and RPl7b is 97 percent; they differ by only 5 amino acid residues. Interestingly, despite the high sequence homology, Rpl7b is detected in both the cytoplasm and the nucleolus, whereas Rpl7a is detected exclusively in the cytoplasm. A site-directed mutagenesis experiment revealed that the change in the amino acid sequence of Rpl7b does not influence its subcellular localization. In addition, introns of RPL7A and RPL7B did not affect the subcellular localization of Rpl7a and Rpl7b. Remarkably, Rpl7b was detected exclusively in the cytoplasm in rpl7a knockout mutant, and overexpression of Rpl7a resulted in its accumulation in the nucleolus, indicating that the subcellular localization of Rpl7a and Rpl7b is influenced by the intracellular level of Rpl7a. Rpl7b showed a wide range of localization patterns, from exclusively cytoplasmic to exclusively nucleolar, in knockout mutants for some rRNA-processing factors, nuclear pore proteins, and large ribosomal subunit assembly factors. Rpl7a, however, was detected exclusively in the cytoplasm in these mutants. Taken together, these results suggest that although Rpl7a and Rpl7b are paralogous and functionally replaceable with each other, their precise physiological roles may not be identical.

• 대한임베디드공학회논문지
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• 제18권4호
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• pp.151-157
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• 2023

RPL (IPv6 Routing Protocol for Low-power Lossy Network) is a standardized routing protocol for LLNs (Low power and Lossy Networks) by the IETF (Internet Engineering Task Force). RPL creates routes and builds a DODAG (Destination Oriented Directed Acyclic Graph) through OF (Objective Function) defining routing metrics and optimization objectives. RPL supports a leaf mode which does not allow any child nodes. In this paper, we propose INFRA-RPL which provides a dynamic leaf mode functionality to a leaf node with the mobility. The proposed protocol is implemented in the open-source IoT operating system, Contiki-NG and Cooja simulator, and its performance is evaluated. The evaluation results show that INFRA-RPL outperforms the existing protocols in the terms of PDR, latency, and control message overhead.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.21.1-21.11
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• 2016

Background: The evaluation of suitable reference genes as normalization controls is a prerequisite requirement for launching quantitative reverse transcription-PCR (RT-qPCR)-based expression study. In order to select the stable reference genes in abalone Haliotis discus hannai tissues (gill and hepatopancreas) under heavy metal exposure conditions (Cu, Zn, and Cd), 12 potential candidate housekeeping genes were subjected to expression stability based on the comprehensive ranking while integrating four different statistical algorithms (geNorm, NormFinder, BestKeeper, and ${\Delta}CT$ method). Results: Expression stability in the gill subset was determined as RPL7 > RPL8 > ACTB > RPL3 > PPIB > RPL7A > EF1A > RPL4 > GAPDH > RPL5 > UBE2 > B-TU. On the other hand, the ranking in the subset for hepatopancreas was RPL7 > RPL3 > RPL8 > ACTB > RPL4 > EF1A > RPL5 > RPL7A > B-TU > UBE2 > PPIB > GAPDH. The pairwise variation assessed by the geNorm program indicates that two reference genes could be sufficient for accurate normalization in both gill and hepatopancreas subsets. Overall, both gill and hepatopancreas subsets recommended ribosomal protein genes (particularly RPL7) as stable references, whereas traditional housekeepers such as ${\beta}-tubulin$ (B-TU) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were ranked as unstable genes. The validation of reference gene selection was confirmed with the quantitative assay of MT transcripts. Conclusions: The present analysis showed the importance of validating reference genes with multiple algorithmic approaches to select genes that are truly stable. Our results indicate that expression stability of a given reference gene could not always have consensus across tissue types. The data from this study could be a good guide for the future design of RT-qPCR studies with respect to metal regulation/detoxification and other related physiologies in this abalone species.

• 한국인터넷방송통신학회논문지
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• 제19권2호
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• pp.91-96
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• 2019

일반적인 무선 센서 네트워크는 연결이 안정적이지 않고 외부 간섭에 민감하며 토폴로지가 유동적이므로 상황에 맞는 효율적인 전송 경로 설정이 중요하다. IETF (Internet Engineering Task Force)의 RPL(IPv6 Routing Protocol for Low-power Lossy Networks) 표준 라우팅 프로토콜은 다양한 환경 변화에 유연하게 대응하기 어려우며 네트워크 토폴로지의 불균형으로 인한 패킷 손실 문제가 상존한다. 다중경로로 여러 부모 노드를 통해 패킷을 전송할 경우 이러한 문제를 부분적으로 해결할 수 있지만 경우에 따라 필요 이상의 timeslot 할당으로 지연시간 증가를 초래할 수 있다. 본 논문에서는 상황에 따라 적절하게 다중경로를 사용하는 적응형 다중경로 RPL 알고리즘을 제안하고, 시뮬레이션을 통해 이의 효율성이 기존 RPL이나 다중경로 RPL에 비해 높음을 보였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권4호
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• pp.2002-2019
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• 2017

RPL routing protocol for low-power and lossy networks is an Internet Engineering Task Force (IETF) recommended IPv6 based protocol for routing over Low power Lossy Networks (LLNs). RPL is proposed for networks with characteristics like small packet size, low bandwidth, low data rate, lossy wireless links and low power. RPL is a proactive routing protocol that creates a Directed Acyclic Graph (DAG) of the network topology. RPL is increasingly used for Internet of Things (IoT) which comprises of heterogeneous networks and applications. RPL proposes a single path routing strategy. The forwarding technique of RPL does not support multiple paths between source and destination. Multipath routing is an important strategy used in both sensor and ad-hoc network for performance enhancement. Multipath routing is also used to achieve multi-fold objectives including higher reliability, increase in throughput, fault tolerance, congestion mitigation and hole avoidance. In this paper, M-RPL (Multi-path extension of RPL) is proposed, which aims to provide temporary multiple paths during congestion over a single routing path. Congestion is primarily detected using buffer size and packet delivery ratio at forwarding nodes. Congestion is mitigated by creating partially disjoint multiple paths and by avoiding forwarding of packets through the congested node. Detailed simulation analysis of M-RPL against RPL in both grid and random topologies shows that M-RPL successfully mitigates congestion and it enhances overall network throughput.

• 한국산업정보학회논문지
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• 제27권6호
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• pp.41-49
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• 2022

IETF(Internet Engineering Task Force)는 저전력 손실 네트워크 환경인 LLNs(Low power and Lossy Networks)의 라우팅 프로토콜로 RPL(IPv6 Routing Protocol for Low-power Lossy Network)을 표준화하였다. RPL은 LLNs에서 요구하는 서비스에 적합한 OF(Objective Function)를 통해 경로를 생성하고 DODAG(Destination Oriented Directed Acyclic Graph)를 구축한다. 기존 연구들은 각 노드의 잔여 에너지를 확인하여 잔여 에너지가 높은 부모를 선택하여 DODAG를 구축하지만 실제 부모 노드가 에너지를 전부 소모하기 전에 DODAG를 떠나고 새로운 DODAG를 구축하는 방식은 없었다. 따라서 본 논문에서는 DODAG에 가입된 노드의 에너지 잔량이 지정된 에너지 한계점 이하로 떨어지면 그 노드가 DODAG를 미리 떠나는 EC-RPL(Enhanced Connectivity-RPL)을 제안한다. 제안된 프로토콜을 오픈소스 사물인터넷 운영체제인 Contiki에서 제공하는 Cooja 시뮬레이터를 사용하여 그 성능을 평가하고 Foren6를 활용하여 제어 메시지 수를 비교한다. 실험 결과 EC-RPL이 기존 RPL 보다 6.9% 낮은 지연시간과 5.8% 낮은 제어 메시지를 사용하며, 패킷 전달 비율은 1.7% 높은 것을 확인할 수 있다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.68-68
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• 2019

In this study, we evaluated the effect of Rodgersia podophylla leave extracts (RPL) on ${\beta}$-catenin level in human cancer cells. RPL dose-dependently inhibited cell proliferation in SW480, A549, MDA-MB-231, PC-3 and AsPC-1 cells. RPL dramatically decreased ${\beta}$-catenin protein level in all cancer cells. However, decreased level of ${\beta}$-catenin mRNA expression was observed in A549 and AsPC-1 cells. In addition, RPL dramatically attenuated cyclin D1 mRNA expression in all cancer cells. MG132 decreased the downregulation of ${\beta}$-catenin protein level induced by RPL in all cancer cells, while RPL-induced downregulation of ${\beta}$-catenin was inhibited by the inhibition of $GSK-3{\beta}$ by LiCl in MDA-MB-231 cells. RPL phosphorylated ${\beta}$-catenin and $GSK-3{\beta}$. In addition, the inhibition of $GSK-3{\beta}$ by LiCl attenuated RPL-induced ${\beta}$-catenin phosphorylation. Based on these findings, RPL may be a potential candidate for the development of chemopreventive or therapeutic agents for human cancer.

• 정보과학회 논문지
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• 제43권11호
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• pp.1233-1244
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• 2016

저전력, 저품질의 네트워크 환경인 LLNs(Low power and Lossy Networks) IoT 네크워크 환경에서는 IETF에서 제안한 IPv6 라우팅 프로토콜인 RPL이 대표적으로 사용된다. RPL은 루프가 존재하지 않는 방향성 비순환 그래프(Directed Acyclic Graph)를 생성하는 것을 목표로 하며, 이를 위해 loop avoidance, loop detection 메커니즘과 문제 발생 시 복구를 위한 DIO Poisoning 메커니즘을 정의하고 있다. 하지만, 기존의 DIO Poisoning은 루프 발생 노드에서 일어난 poisoning이 해당 노드의 서브트리로 전파되어 복구 시간과 컨트롤 메시지가 증가하는 문제점을 가지고 있다. 본 논문에서는 RPL 기반 IoT 무선 네트워크에서 루프 복구 과정 시 서브 트리의 라우팅 오버헤드가 추가로 발생할 수 있는 현상을 보완한 효율적인 경로 복구 기법을 제안한다. 개선된 RPL 루프 복구 과정에서는 기존 선호 부모로 선택될 수 없던 경로를 활용하여 빠르게 복구함으로써 새로운 경로설정을 위한 컨트롤 패킷 트래픽과 경로 복구 시간을 줄인다. 시뮬레이션을 사용하여 제안한 프로토콜이 기존 프로토콜에 비해 복구 시간 단축과 컨트롤 패킷의 감소를 통한 복구 성능을 향상시킬 수 있음을 알 수 있었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.94-94
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• 2019

In this study, we elucidated the anti-inflammatory mechanisms of leaves extracts from Rodgersia podophylla (RPL) in RAW264.7 cells. RP-L significantly inhibited the production of the proinflammatory mediators such as NO, iNOS, IL-$1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. RPL increased HO-1 expression in RAW264.7 cells, and the inhibition of HO-1 by ZnPP reduced the inhibitory effect of RPL against LPS-induced NO production in RAW264.7 cells. Inhibition of p38, ROS and $GSK3{\beta}$ attenuated RPL-mediated HO-1 expression. Inhibition of ROS inhibited p38 phosphorylation and $GSK3{\beta}$ expression induced by RPL. In addition, inhibition of $GSK3{\beta}$ blocked RPL-mediated p38 phosphorylation. RPL induced nuclear accumulation of Nrf2, and Inhibition of p38, ROS and $GSK3{\beta}$ abolished RPL-mediated nuclear accumulation of Nrf2. Furthermore, RPL blocked LPS-induced degradation of $I{\kappa}B-{\alpha}$ and nuclear accumulation of p65. RP-L also attenuated LPS-induced phosphorylation of ERK1/2 and p38. Our results suggest that RPL exerts potential antiinflammatory activity by activating ROS/$GSK3{\beta}$/p38/Nrf2/HO-1 signaling and inhibiting NF-${\kappa}B$ and MAPK signaling in RAW264.7 cells. These findings suggest that RPL may have great potential for the development of anti-inflammatory drug.