• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.12
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• pp.1679-1686
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• 2019

The main purpose of this research is to propose an approach for solving the packet loss problem by quickly adapting to topology change when nodes move in RPL-based IoT environment. In order to enhance mobility, every node is aware of the mobility of its neighbor nodes and quantifies the mobility level based on the number of control messages and all received packets. According to the mobility level, the DIO timer is changed. The proposed approach allows nodes to change their DIO timers according to their mobility levels to adapt topology changes and update paths to the sink. The performance of the proposed approach is evaluated using a Contiki-based Cooja simulator in various moving speeds. The simulation results show that the proposed approach copes with mobility scenarios better than the standard RPL by ascertaining that the packet delivery ratio is improved by 31.03%.

• Journal of KIISE
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• v.42 no.10
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• pp.1268-1279
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• 2015

In order to manage a large number of devices connected to the Internet of Things (IoT), the Internet Engineering Task Force (IETF) proposed the IPv6 Routing Protocol for Low Power and Lossy Networks (RPL). The route of the RPL network is generated through the use of an Objective Function (OF) that is suitable for the service that is required for the IoT network. Since the route of the RPL network is conventionally simply chosen only by considering the link quality between the nodes, it is sensible to seek an OF that can also provide better Quality of Service (QoS). In previous studies, the end-to-end delay might possibly be sub-optimal because they only deal with problems related to the reduction of energy consumption and not to the link quality on the path to the sink node. In this study, we propose a scheme that reduces the end-to-end delay but also gives full consideration to both the quality on the entire route to the destination and to the expected lifetime of nodes with bottlenecks from heaped traffic. Weighting factors for the proposed scheme are chosen by experiments and the proposed scheme can reduce the end-to-end delay and the energy consumption of previous studies by 20.8% and 10.5%, respectively.

• Journal of Practical Engineering Education
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• v.9 no.1
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• pp.57-68
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• 2017

The purpose of this study is to analyze the actual preparation process and outcomes of applying RPL in University Dual System based on KOREATECH's case. A comprehensive literature review on prior research and cases was described in this study to assist Korean educational institutions who might be interested in adopting RPL into their organizations. Furthermore, three different levels of support were suggested based on the results of this study for successful adoption of RPL in Korea. Support from national system level, sociocultural level, and university level should be considered carefully for the success of RPL.

• Journal of Korea Multimedia Society
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• v.21 no.9
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• pp.1090-1098
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• 2018

MQTT is a lightweight messaging protocol that can be used for low power IoT devices. The MQTT basically uses single MQTT broker to indirectly share message information between publishers and subscribers. This approach has a weakness in regard to traffic overflow, connection fault, security, etc. In this paper, we propose a distributed MQTT broker architecture that solves the problems in single MQTT broker structure. The distributed MQTT broker architecture is expected to support new application services that cannot be supported by a conventional MQTT architecture. We have designed and implemented a distributed MQTT broker architecture based on the RPL protocol that has been widely used for IoT network. The experiment results show that the proposed MQTT broker architecture represents better publishing/subscribing latency and network stability than the conventional MQTT broker architecture.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.1
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• pp.196-201
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• 2018

Recently, various infrastructure-less IoT networking schemes have been studied to construct local IoT networks based on self-organization. This is, because RPL protocol, which is to support infrastructure based network construction is used to construct local IoT networks. Thus, a self-organization networking and ad hoc path between client and server in local IoT networks is not supported in basis RPL protocol. In this paper, we propose a self-organization networking scheme which support infrastructure-less based IoT network construction in low-power and lossy network based IoT environments consisting of IoT devices with the constrained feature, such as low power, the limited transmission rate and low computing capacity. Through simulation, we show that the proposed self-organization networking scheme improves the performance, in terms of the number of packets generated for end-to end data transmission and the end-to-end delay, compared to basis RPL protocol.

• Korean Journal of Medicinal Crop Science
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• v.18 no.3
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• pp.173-179
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• 2010

This study describes an efficient approach to the development of DNA markers for use in distinguishing the Scrophularia species that have been used as useful medicinal crops. In order to distinguish Scrophularia species, DNA sequences of rpl-5 region in mitochondrial DNA of Scrophularia species were analysed for detecting sequence variations, and the PCR-RFLP method was applied for developing practicable DNA marker patterns. Several DNA variations were detected by the sequence comparison of rpl-5 region among Scrophularia species. Genetic relationship analysis of Scrophularia species was carried out based on these DNA variations. DNA variations of rpl-5 region were revealed that it was significantly efficient in genetic relationship analysis of Scrophularia species. In addition, Scrophularia species tested in this study were completely discriminated by four polymorphic genotypes by PCR-RFLP combined with Tsp509 I (^AATT) restriction enzyme. Our results suggested that DNA sequence variations of rpl-5 region were sufficiently useful for genetic relationship analysis of Scrophularia species. Polymorphic genotypes by PCR-RFLP using the Tsp509 I enzyme will be useful for discrimination of Scrophularia species as a practicable DNA markers.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.5
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• pp.123-128
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• 2019

The Internet Engineering Task Force (IETF) proposed IPv6 Routing Protocol for Low-power Lossy Network (RPL) as a routing protocol for Low Power and Lossy Networks (LLN). In RPL networks, only a few parent nodes are connected to many child nodes, which is called Thundering Herd Phenomenon. To solve this problem, it has been considered to limit the maximum number of child nodes connected per node using the CNC (Child Number Count) parameter. However, the problem remains that some parent nodes can be attached with as many as the maximum number of child nodes. How to determine the maximum CNC value is yet another problem. Therefore, in this paper, we propose an algorithm that evenly distributes the number of child nodes connected per node, to solve the Thundering Herd Phenomenon problem. The performance of the proposed algorithm is compared with that of the conventional RPL using CNC. As a result, we showed that the proposed algorithm performs better in terms of load balancing.

• Journal of radiological science and technology
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• v.46 no.3
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• pp.219-229
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• 2023

In this study, basic characteristics such as reproducibility, linearity, and directionality of RPL glass dosimeters were evaluated to improve the reliability of dose evaluation through RPL glass dosimeters, and uncertainty elements such as sensitivity by glass element and magazine slot sensitivity were evaluated. Using a mathematical model to calibrate the measured values of the RPL glass dosimeter, the measurement uncertainty was calculated assuming an example. As a result of the characteristic evaluation, the RPL glass dosimeter showed excellent performance with a standard deviation of ±1% (1 SD) for the reproducibility of the reading process, a coefficient of determination for linearity of 0.99997. And the read-out of the RPL glass dosimeter are affected by the circular rotation direction of the glass dosimeter during irradiation, fading according to the period after irradiation, the number of laser pulses of the reader, and response degradation due to repeated reading, it is judged that measurement uncertainty can be reduced by irradiation and reading in consideration of these factors. In addition, it was confirmed that the dose should be determined by calculating the correction factors for the sensitivity of each element and, the sensitivity of each reading magazine slot. It is believed that the reliability of dosimetry using glass dosimeters can be improved by using a mathematical model for correction of glass dosimeter readings and calculating measurement uncertainty.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.12
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.