• Molecules and Cells
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• 제27권1호
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• pp.99-103
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• 2009

Ribose-5-phosphate isomerase A (RpiA) plays an important role in interconverting between ribose-5-phosphate (R5P) and ribulose-5-phosphate in the pentose phosphate pathway and the Calvin cycle. We have determined the crystal structures of the open form RpiA from Vibrio vulnificus YJ106 (VvRpiA) in complex with the R5P and the closed form with arabinose-5-phosphate (A5P) in parallel with the apo VvRpiA at $2.0{\AA}$ resolution. VvRpiA is highly similar to Escherichia coli RpiA, and the VvRpiA-R5P complex strongly resembles the E. coli RpiA-A5P complex. Interestingly, unlike the E. coli RpiA-A5P complex, the position of A5P in the VvRpiA-A5P complex reveals a different position than the R5P binding mode. VvRpiA-A5P has a sugar ring inside the binding pocket and a phosphate group outside the binding pocket: By contrast, the sugar ring of A5P interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues; the phosphate group of A5P interacts with two water molecules, W51 and W82.

• The Plant Pathology Journal
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• 제30권3호
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• pp.254-260
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• 2014

Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBSLRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.26-26
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• 2015

Phytophthora infestans is the causal agent of potato and tomato late blight, one of the most devastating plant diseases. P. infestans secretes effector proteins that are both modulators and targets of host plant immunity. Among these are the so-called RXLR effectors that function inside plant cells and are characterized by a conserved motif following the N-terminal signal peptide. In contrast, the effector activity is encoded by the C terminal region that follows the RXLR domain. Recently, I performed in planta functional profiling of different RXLR effector alleles. These genes were amplified from a variety of P. infestans isolates and cloned into a Potato virus X (PVX) vector for transient in planta expression. I assayed for R-gene specific induction of hypersensitive cell death. The findings included the discovery of new effector with avirulence activity towards the Solanum bulbocastanum Rpi-blb2 resistance gene. The Rpi-blb2 encodes a protein with a putative CC-NBS-LRR (a coiled-coil-nucleotide binding site and leucine-rich repeat) motif that confers Phytophthora late blight disease resistance. We examined the components required for Rpi-blb2-mediated resistance to P. infestans in Nicotiana benthamiana. Virus-induced gene silencing was used to repress candidate genes in N. benthamiana and to assay against P. infestans infections. NbSGT1 was required for disease resistance to P. infestans and hypersensitive responses (HRs) triggered by co-expression of AVRblb2 and Rpi-blb2 in N. benthamiana. RAR1 and HSP90 did not affect disease resistance or HRs in Rpi-blb2-transgenic plants. To elucidate the role of salicylic acid (SA) in Rpi-blb2-mediated resistance, we analyzed the response of NahG-transgenic plants following P. infestans infection. The increased susceptibility of Rpi-blb2-transgenic plants in the NahG background correlated with reduced SA and SA glucoside levels. Furthermore, Rpi-blb2-mediated HR cell death was associated with $H_2O_2$, but not SA, accumulation. SA affects basal defense and Rpi-blb2-mediated resistance against P. infestans. These findings provide evidence about the roles of SGT1 and SA signaling in Rpi-blb2-mediated resistance against P. infestans.

• 방사성폐기물학회지
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• 제21권3호
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• pp.371-381
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• 2023

Evaluating the effectiveness of the radiation protection measures deployed at the Centralized Radioactive Waste Management Facility in Ghana is pivotal to guaranteeing the safety of personnel, public and the environment, thus the need for this study. Radiagem^TM 2000 was used in measuring the dose rate of the facility whilst the personal radiation exposure of the personnel from 2011 to 2022 was measured from the thermoluminescent dosimeter badges using Harshaw 6600 Plus Automated TLD Reader. The decay store containing scrap metals from dismantled disused sealed radioactive sources (DSRS), and low-level wastes measured the highest dose rate of 1.06 ± 0.92 µSv·h^-1. The range of the mean annual average personnel dose equivalent is 0.41-2.07 mSv. The annual effective doses are below the ICRP limit of 20 mSv. From the multivariate principal component analysis biplot, all the personal dose equivalent formed a cluster, and the cluster is mostly influenced by the radiological data from the outer wall surface of the facility where no DSRS are stored. The personal dose equivalents are not primarily due to the radiation exposures of staff during operations with DSRS at the facility but can be attributed to environmental radiation, thus the current radiation protection measures at the Facility can be deemed as effective.

• 대한방사선방어학회:학술대회논문집
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• 대한방사선방어학회 2011년도 추계 학술발표회 및 심포지엄
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• pp.280-281
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• 2011

• Genomics & Informatics
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• 제9권1호
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• pp.39-43
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• 2011

MicroRNAs (miRNAs) are recently discovered small RNA molecules usually resulting in translational repression and gene silencing. Despite the fact that specific cloning of small RNA's is a method in practice, computational identification of miRNA's has been a major focus recent days, since is a rapid process following AB initio and sequence alignment methods. Here we developed new software called MiRPI that aims to identify the highly conserved miRNAs without any mismatches from given fasta formatted gene sequences by using non-repeated miRNA dataset of the user's interest. The new window embedded with the software is used to identify the targets for inputted mature miRNAs in the mRNA sequences. Also MiRPI is designed to measure the precursor miRNA statistics, majorly focusing the Adjusted Minimum Folding free Energy (AMFE) and Minimum Folding free Energy Index (MFEI), the most important parameters in miRNA confirmation. MiRPI is developed by PERL (Practical Extraction and Report Language) and Tk (Tool kit widgets) scripting languages. It is user friendly, portable offline software that works in all windows OS, sized to 3 MB.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 추계학술대회 논문집 전력기술부문
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• pp.170-173
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• 2006

2006년 9월 1일에 제정된 독립사업부제는 각 단위별로 자율적인 운영권을 가지면서 비용 등에 대해서도 스스로 책임지도록 하는 제도이다. 배전계통의 사업구역 분할로 시작된 배전 사업부제는 독립 재무제표를 통해 경영실적이 드러나기 때문에 경영혁신과 원가절감 경쟁을 유도할 수 있다는 게 특징이다. 이런 체제의 변화는 기업의 규제 시스템에도 새로운 변화가 필요하게 될 것이다. 하지만 서로 다른 규모와 환경을 가진 사업부들을 하나의 기준으로 평가하는 것은 어려운 일이다. 그래서 본 논문에서는 배전 사업부제로 인해 나눠진 사업부들의 경제성과 상대적인 가치를 평가할 수 있는 방법 중의 하나로 세계적으로 널리 쓰이고 있는 Data Envelopment Analysis를 제안한다. 그 결과를 통해서 사업부에 대한 경영 평가나 각 사업부가 더 높은 경제성을 갖출 수 있는 RPI-X regulation을 수행하는데 하나의 모델로서 사용될 수 있을 것이다. RPI-X regulation에서 X값을 결정하는 것은 비용 경쟁력을 향상시키는 비율을 나타내기 때문에 적절한 X값을 찾는 것이 가장 중요하다고 하겠다. 이 때 여러 개의 입력과 여러 개의 출력을 가지는 문제에 대해서 쉽게 결과를 나타낼 수 있는 Data Envelopment Analysis를 X를 결정하는 방법으로 사용하였다. 그리고 사업부들의 노동력과 선로긍장과 용량 등의 값을 입력으로 하고 고객호수와 판매량을 결과로 하여 RPI-X regulation의 적절한 X값을 찾는 예를 통하여 이 방법이 실제적으로 어떤 효과가 있는지 확인해보았다. 본 논문에서는 분화된 사업부들에 대하여 Data Envelopment Analysis 기법을 이용하여 RPI-X regulation을 수행하였고 결과적으로 본 논문에서 제안한 방법을 통하여 분화된 배전 시스템에도 적용할 수 있는 가능성을 보였다.

• 구강회복응용과학지
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• 제30권3호
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• pp.259-264
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• 2014

역행성 임플란트 근단병소 주위염은 임상증상을 동반한 근단부의 방사선 투과성 병소로 정의되며 임플란트 식립부위 또는 인접한 치아의 잔존하고 있는 감염에서 유래한 세균감염, 골 삭제시의 열 발생 등의 다양한 원인에 의해 야기될 수 있고, 다양한 치료방법으로 임플란트를 제거하지 않고 유지할 수 있는 증례들이 보고되고 있다. 본 증례에서는 근관 치료 실패로 발치한 상악 우측 제2소구치 부위에 식립한 임플란트에서 발생한 역행성 임플란트 근단병소 주위염을 표면의 detoxification과 차폐막과 골이식재를 동반한 골유도재생술로 해결하여 기능을 회복하였으며, 7년간 장기적으로 안정적으로 유지되고 있는 증례에 대해 보고하고자 한다.

• 한국컴퓨터정보학회논문지
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• 제29권6호
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• pp.223-233
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• 2024

본 연구에서는 정보 제시, 시스템 상호 작용, 소셜 커뮤니티 기능의 역할에 초점을 맞춰 개인화 추천 시스템이 전자 상거래에서 고객 충성도에 미치는 영향을 조사합나다. 이러한 요소들이 플로 상태, 입소문(WOM), 재구매 의도(RPI)에 어떤 영향을 미치는지 살펴봅니다. 이 연구는 구조방정식 모델(SEM)과 500명의 응답자로부터 수집한 데이터를 SPSS와 AMOS를 사용하여 세 가지 개인화 측면이 모두 플로상태를 크게 향상시키고, 이는 다시 WOM과 RPI에 긍정적인 영향을 미친다는 사실을 발견했습니다. 시스템 상호작용은 WOM과 RPI를 직접적으로 향상시키는 반면, 정보 제공과 소셜 커뮤니티 기능은 이러한 충성도 측정치 중 하나에만 영향을 미치는 것으로 나타났습니다. 플로 상태는 개인화 요소와 충성도 결과 사이의 관계를 매개합니다. 이러한 연구 결과는 전자 상거래 플랫폼이 고객 충성도를 높이기 위해 시스템 상호작용을 개선하고 소셜 커뮤니티 기능을 포함해야 함을 시사합니다.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1122-1132
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• 2018

In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions ($50^{\circ}C$, pH 8.0, and 1 mM $Ca^{2+}$) were obtained to maximize the potential of OsRpiB in preparing $\text\tiny{L}$-rhamnulose. The catalytic properties of OsRpiB, including $K_m$, $k_{cat}$, and catalytic efficiency ($k_{cat}/K_m$), were determined as 43.47 mM, $129.4sec^{-1}$, and 2.98 mM/sec. The highest conversion rate of $\text\tiny{L}$-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose by OsRpiB biocatalysis.