• Title/Summary/Keyword: RPH1

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A $G_{4}$ Sequence within PHR1 Promoter Acts as a Gate for Cross-Talks between Damage-Signaling Pathway and Multi-Stress Response

  • Jang, Yeun-Kyu;Kim, Eun-Mi;Park, Sang-Dai
    • Animal cells and systems
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    • v.6 no.3
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    • pp.271-275
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    • 2002
  • Rph1 and Gisl are damage-responsive repressors involved in PHR1 expression. They have two $C_{2}$H/ sub 2/ zinc finger motifs as putative DNA binding domains and N-terminal conserved domain with unknown function. They are also found in the human retinoblastoma binding protein 2 and the mouse jumonji- encoded protein. The repressors are able to bind to A $G_{4}$ sequence within a 39-bp sequence called upstream repressing sequence of PHR1 promoter (UR $S_{PHR1}$) responsible for the damage-response of PHR1. We report here that Rph1 is predominantly localized in the nucleus as examined by fluorescence microscopic analysis with GFP-Rph1 fusion protein. On the basis of the fact that the A $G_{4}$ sequence that is recognized by Rph1 and Gisl is also recognized by Msn2 and Msn4 in a process of stress response, we a1so tried to examine the in vivo function of A $G_{4}$ and the role of Msn2 and Msn4 in PHR1 expression. Our results demonstrate that Msn2 and Msn4 are actually required for the basal transcription of PHR1 expression but not for its damage induction. When A $G_{4}$ sequence was inserted into the minimal promoter of the cyc1-LacZ reporter, the increased LacZ expression was observed indicating its involvement in transcriptional activation. The data suggest that the A $G_{4}$ is primarily required for basal transcriptional activation of PHR1 or CYC1 promoter through the possible involvement of Msn2 and Msn4. However, since the A $G_{4}$ is also involved in the repression of PHR1 via Rphl and Gisl, it is proposed that A $G_{4}$ functions as either URS or upstream activating sequence (UAS) depending on the promoter context.t.

Effect of Gravity Perturbation on the Axis Specification in the Egg of Xenopus laevis (중력 작용의 변경 효과가 무미양서류 Xenopus laevis 수정란의 축형성에 미치는 영향)

  • 정해문;한평림
    • The Korean Journal of Zoology
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    • v.27 no.3
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    • pp.127-136
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    • 1984
  • The specification of dorsal/ventral axis in the egg of Xenopus laevis was investigated as a series of oblique orientation to gravity by tilt and clinostat. The results are as follows. (1) If the eggs were oriented, in the early period after fertilization, to novel gravity by $15^\\circ, 30^\\circ, 45^\\circ$ and $60^\\circ$ tilt until gastrula stage, the site of involution was usually formed in the OpG side (the side opposing gravity). As the degree of tilt was raised from $15^\\circ to 60^\\circ$, the rate of relocation of the involution site was proportionally increased. (2) When UV-irradiated eggs were tilted period to first cleavage by $15^\\circ, 30^\\circ, 45^\\circ$ and $6\^\\circ$, the effect of UV syndrome was rescued, and the extent of rescue was propotional to the tilt degree. (3) The fertile eggs were loaded on the clinostats of several speeds. In the range of low speeds between $0.45 \\sim 9.0$ rph, the location of dorsal lip was dependent on the direction of rotation, and in $40 \\sim 360$ rph, lip was formed at a random position. In addition, some of the tadpoles experienced with clinostat showed the typical syndrome of "dorsal axis reduction". The above results were discussed regarding the mechanism of the establishment of dorsal/ventral palarity. palarity.

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Effects of Detention Time and Disc Speed on the Treatment Efficiencies of RBC (체류시간(滯留時間)과 원판회전속도(圓板回轉速度)가 회전원판생물막법(回轉圓板生物膜法)의 처리효율(處理効率)에 미치는 영향(影響))

  • Kim, Sang Yong
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.4 no.3
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    • pp.1-9
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    • 1984
  • A mult-stage rotating biological contactor process was evaluated by using the mixtures of nightsoil and sewage as influent substrate. An emphasis was concentrated on the BOD removal efficiency at each stage of the process with respect to hydraulic detention times, rotating speeds of the disc and influent organic substrate concentrations. The results indicated that the process was found to be economically feasible when operated at hyduraulic detention time of 2 hours and disc rotating speed of 3 RPH. As to treatment efficiency, BOD removal efficiency of 40 to 50 percent could be obtained at the first stage regardless of influent organic substrate concentrations. The overall BOD removal efficiency of the process was found to be 88 to 90 percent at even high organic loading of $2.0kgBOD/m^3{\cdot}day$.

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