• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10513-10524
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• 2015

Background: Metastasis is a major reason for poor prognosis in patients with cancer, including hepatocellular carcinoma (HCC). A salient feature is the ability of cancer cells to colonize different organs. Long non-coding RNAs (lncRNAs) play important roles in numerous cellular processes, including metastasis. Materials and Methods: In this study, the lncRNA expression profiles of two HCC cell lines, one with high potential for metastasis to the lung (HCCLM3) and the other to lymph nodes (HCCLYM-H2) were assessed using the Arraystar Human LncRNA Array v2.0, which contains 33,045 lncRNAs and 30,215 mRNAs. Coding-non-coding gene co-expression (CNC) networks were constructed and gene set enrichment analysis (GSEA) was performed to identify lncRNAs with potential functions in organ-specific metastasis. Levels of two representative lncRNAs and one representative mRNA, RP5-1014O16.1, lincRNA-TSPAN8 and TSPAN8, were further detected in HCC cell lines with differing metastasis potential by qRT-PCR. Results: Using microarray data, we identified 1,482 lncRNAs and 1,629 mRNAs that were differentially expressed (${\geq}1.5$ fold-change) between the two HCC cell lines. The most upregulated lncRNAs in H2 were RP11-672F9.1, RP5-1014O16.1, and RP11-501G6.1, while the most downregulated ones were lincRNA-TSPAN8, lincRNA-CALCA, C14orf132, NCRNA00173, and CR613944. The most upregulated mRNAs in H2 were C15orf48, PSG2, and PSG8, while the most downregulated ones were CALCB, CD81, CD24, TSPAN8, and SOST. Among them, lincRNA-TSPAN8 and TSPAN8 were found highly expressed in high lung metastatic potential HCC cells, while lowly expressed in no or low lung metastatic potential HCC cells. RP5-1014O16.1 was highly expressed in high lymphatic metastatic potential HCC cell lines, while lowly expressed in no lymphatic metastatic potential HCC cell lines. Conclusions: We provide the first detailed description of lncRNA expression profiles related to organ-specific metastasis in HCC. We demonstrated that a large number of lncRNAs may play important roles in driving HCC cells to metastasize to different sites; these lncRNAs may provide novel molecular biomarkers and offer a new basis for combating metastasis in HCC cases.

• Tuberculosis and Respiratory Diseases
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• v.84 no.4
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• pp.282-290
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• 2021

Background: Radial probe endobronchial ultrasound-guided transbronchial lung biopsy (RP-EBUS-TBLB) has improved the diagnostic yield of bronchoscopic biopsy of peripheral pulmonary lesions (PPLs). The diagnostic yield and complications of RP-EBUS-TBLB for PPLs vary depending on the technique, such as using a guide sheath (GS) or fluoroscopy. In this study, we investigated the utility of RP-EBUS-TBLB using a GS without fluoroscopy for diagnosing PPLs. Methods: We retrospectively reviewed data from 607 patients who underwent RP-EBUS of PPLs from January 2019 to July 2020. TBLB was performed using RP-EBUS with a GS without fluoroscopy. The diagnostic yield and complications were assessed. Multivariable logistic regression analyses were used to identify factors affecting the diagnostic yields. Results: The overall diagnostic accuracy was 76.1% (462/607). In multivariable analyses, the size of the lesion (≥20 mm; odds ratio [OR], 2.06; 95% confidence interval [CI], 1.27-3.33; p=0.003), positive bronchus sign in chest computed tomography (OR, 2.30; 95% CI, 1.40-3.78; p=0.001), a solid lesion (OR, 2.40; 95% CI, 1.31-4.41; p=0.005), and an EBUS image with the probe within the lesion (OR, 6.98; 95% CI, 4.38-11.12; p<0.001) were associated with diagnostic success. Pneumothorax occurred in 2.0% (12/607) of cases and chest tube insertion was required in 0.5% (3/607) of patients. Conclusion: RP-EBUS-TBLB using a GS without fluoroscopy is a highly accurate diagnostic method in diagnosing PPLs that does not involve radiation exposure and has acceptable complication rates.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.3
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• pp.1-11
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• 2016

The present study aims to evaluate transfer resistance factors in railway stations and draw new implications. The data used for the model estimation are RP (revealed preference) data and SP (stated preference) data. Two types of models were used for the analysis: integration model which combined line-haul stage, transfer stage and shuttle stage and separation model which assessed the three stages separately. The results revealed that while bulk freight shippers mainly focused on line-haul stage, they put emphasis on transfer stage as well. It's especially notable that transfer stage was considered more important than shuttle stage. Therefore, in future transportation policies concerning rail freight, it would be crucial not only to enhance the competitiveness of line-haul stage but also make improvements in transfer stage regarding railway stations.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.6 no.2
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• pp.57-69
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• 2007

The most important problem in logistic activities may be to decrease the transportation efficiency due to the traffic congestion in urban areas. The traffic congestion reduces the average travel speed of freight vehicles, and then increases the travel time. These problems can lead the logistic system to be inefficient. As a result, it causes an increase of transportation costs. In addition, the increased cost is a main barrier for the transition to an advanced logistic system. This study focuses on the analysis of key factors choosing commercial freight vehicles using Logistic regression-analysis with RP (Revealed Preference) data to solve the increase of private freight cars and transportation costs. Additionally, this paper presents policies to promote good use of commercial freight vehicles based on the results of this study.

• The Journal of Korean Academy of Prosthodontics
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• v.57 no.3
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• pp.211-218
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• 2019

Purpose: The purpose of the present study was to compare the accuracy of four different metal copings fabricated by CAD/CAM technology and to evaluate clinical effectiveness. Materials and methods: Composite resin tooth of the maxillary central incisor was prepared for a metal ceramic crown and duplicated metal die was fabricated. Then scan the metal die for 12 times to obtain STL files using a confocal microscopy type oral scanner. Metal copings with a thickness of 0.5 mm and a cement space of $50{\mu}m$ were designed on a CAD program. The Co-Cr metal copings were fabricated by the following four methods: Wax pattern milling & Casting (WM), Resin pattern 3D Printing & casting (RP), Milling & Sintering (MS), Selective laser melting (SLM). Silicone replica technique was used to measure marginal and internal discrepancies. The data was statistically analyzed with One-way analysis of variance and appropriate post hoc test (Scheffe test) (${\alpha}=.05$). Results: Mean marginal discrepancy was significantly smaller in the Group WM ($27.66{\pm}9.85{\mu}m$) and Group MS ($28.88{\pm}10.13{\mu}m$) than in the Group RP ($38.09{\pm}11.14{\mu}m$). Mean cervical discrepancy was significantly smaller in the Group MS than in the Group RP. Mean axial discrepancy was significantly smaller in the Group WM and Group MS then in the Group RP and Group SLM. Mean incisal discrepancies was significantly smaller in the Group RP than in all other groups. Conclusion: The marginal and axial discrepancies of the Co-Cr coping fabricated by the Wax pattern milling and Milling/Sintering method were better than those of the other groups. The marginal, cervical and axial fit of Co-Cr copings in all groups are within a clinically acceptable range.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.10
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• pp.46-55
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• 1996

As industrial standard data, the STL format which approximates three dimensional CAD model to triangular facets, is used for RP(Rapid Prototyping) system in recent days. Because most RP system take the only form of two dimensional line segments as an input stream inspite of its imperfectness while converting into STL format, a CAD model is converted into a standard industrial format which is composed of many triangular facets. The error verifying process is composed of four main steps, and these are 1) Remove facets with two or more vertices equal to each other. 2) Fix overlapping error such as more than three facets adjacent to anedge. 3) Fill holes in the mesh by using Delaunay triangulation method. 4) Correct the wrong direction and normal vectors. This paper is concerned with serching the mentioned errors in advance and modifying them.

• Journal of the Korean Society of Industry Convergence
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• v.5 no.2
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• pp.141-146
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• 2002

Stereolithography is the best known as rapid prototyping system. It uses the STL format data which is generated from CAD system. In this study, One of the main function of this developed CAM system deals with shape modification which divide a shape into two parts or more. The cross section of a STL part by a z-level is composed with nested or single polygonal closed loop. In order to make RP product, closed loops must fill with triangular facets from SSET and recover sliced triangular facets which is located normal direction to the cross sectional plane. The system is development by using Visuall C++ compiler in the environment of pentium PC. Operating system is Windows NT workstaion from Micro-Soft.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1996.04a
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• pp.597-601
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• 1996

Nowadays, the STL format, industrial standard data, which approximates three dimensional CAD model to triangular facets, is used for RP(Rapid Prototyping) system. Because most RP machine is accpted to only two dimensional line segments, but some STL translators are sometimes poorly implemented. The error verifying process is as follows. 1) Remove facets with two or more vertices equal to each other. 2) Fix overlapping error such as more than three facets adjacent to an edge. 3) Fill holes in the mesh by using Delaunay triangulation method. 4) Repair wrong direction and value of normal vectors. This paper is concerned with searching the mentioned errors in advance and modifying them.

• Journal of Ship and Ocean Technology
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• v.10 no.1
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• pp.38-46
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• 2006

The objective of this paper is to summarize current ultra-deepwater (i.e., up to 3,500 meters water depth) pipeline mechanical design methodologies as part of the limit state design. The standard mechanical design for ultra-deepwater pipelines in the Gulf of Mexico (GOM) is based on API RP 1111. API code also has been used for deepwater projects in west Africa. DNV code OS-F101 was mostly used for deepwater projects in offshore Brazil and Europe. Some pipeline designs in the GOM have started to incorporate parts of the DNV design methodology. A discussion of failure under collapse only and combined loading (i.e. pressure + bending) is presented. The best design criteria are obtained from physical full-scale collapse testing. The comparison of the physical test data and collapse calculations using the DNV and API codes will be presented. It was found that the conservatism still exists in the collapse prediction for ultra-deepwater pipeline using modem design codes such as DNV OS-F101 and API RP 1111.

• Korean Journal of Computational Design and Engineering
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• v.3 no.1
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• pp.15-21
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• 1998

Several adaptive and direct slicing methods have been developed to make the slice data for RP parts with better accuracy and speed. This research deals with a new adaptive slicing algorithm that shows drastic improvement in computing time for calculating the slices of a part. First, it uses less number of sampling points fur each slice in determining the thickness of the next slice. Secondly, the idea of contour map is utilized to determine the optimal sampling point on each slice. Thirdly, the calculation efficiency is further improved by introducing vertical character lines of the given part. The results in terms of accuracy and speed are compared with the existing methods.