• BMB Reports
• /
• 제40권1호
• /
• pp.22-28
• /
• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• Water Engineering Research
• /
• 제6권1호
• /
• pp.17-30
• /
• 2005

The bed evolution of the stretch of the River Rhine between km-812.5 and km-821.5 is characterised by general bed degradation as a result of the river training works and dredging activities of the last two centuries. The degradation of the river bed affects the water levels, and so the navigation conditions. To combat the erosion of the river bed with the aim to keep up the shipping traffic and to avoid the ecological system damages due to water level reductions, sand-gravel-mixtures were added to the river (so called artificial grain feeding activities). This paper presents the results of an application of a graded sediment transport model in order to study morpholodynamical characteristics due to artificial grain feeding activities in the river stretch. The finite element code TELEMAC2D was used for flow calculation by solving the 2D shallow water equation on non-structured grids. The sediment transport module SISYPHE has been developed for graded sediment transport using a multiple layer model. The needs to apply such graded sediment transport approaches to study morphological processes in the domain are discussed. The calculations have been carried out for the case of middle water flow and different size-fraction distributions. The results show that the grain feeding process could be well simulated by the model.

• International Journal of Contents
• /
• 제8권3호
• /
• pp.34-41
• /
• 2012

The concept of vegetation freeboard equivalence (VFE) is presented from the comparison between the rise in stage with/without vegetation and the freeboard height under design discharge conditions. In South Korea, the freeboard height of large, medium and small rivers is defined as a function of river discharge. Two models are used for this analysis of flood stage with and without vegetation: the 1-D model HEC-RAS and the 2-D model RMA-2. Both models are applied to three river study sites of the Geum River in South Korea as representative sites for a large, a medium and a small river. The analysis shows that without vegetation, both models provide comparable results and the calculated results are in very good agreement with the design configuration. The vegetation effects on the medium river are less significant, and the freeboard is adequate to contain the rise in stage from the added floodplain vegetation in large rivers. The concept of vegetation freeboard equivalence is therefore useful for the analysis of flood river stages after the restoration of channels with increased floodplain vegetation.

• 한국방재학회 논문집
• /
• 제8권1호
• /
• pp.117-123
• /
• 2008

본 연구에서는 하천의 유지유량을 결정함에 있어서 어류의 서식처를 고려한 최적의 유량을 산정하기 위하여 한강수계의 주요지천에 1차원 물리서식처 모의 모형인 PHABSIM과 2차원 물리서식처 모의 모형인 River2D를 적용하였다. 또한 대상어종의 성장단계별(성어기 산란기) 두 모형의 가중가용면적(WUA)에 대하여 비교검토하였으며 어류서식조건을 고려한 최적유량 값을 산정하였다. 모의결과 1차원 모형과 2차원 모형에서 가중가용면적(WUA)의 상관분석 결과 $0.87{\sim}0.99$로 분석되었고 어류서식조건을 고려한 최적유량 역시 문막 지점의 성어기와 달천지점의 산란기를 제외하고 $3m^3/s{\sim}5m^3/s$의 차이로 큰 차이를 보이지 않았다.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
• /
• pp.437-441
• /
• 2009

현대사회는 하천에서부터 시작되었다고 할 정도로 우리 삶의 중심에 있는 자연의 중요한 일부라고 할 수 있다. 하천은 단순한 치수와 이수뿐만 아니라 다양한 자연 그대로의 환경적 잠재 기능을 복원하는 방향으로 전환되고 있다. 이러한 하천 복원의 필수적 요소는 식생이나 이것은 하천의 흐름저항을 크게 하여 홍수시 수위를 증가시키는 요인이 된다. 따라서 식생에 따른 조도계수의 변화와 흐름저항으로 인한 수리학적 특성을 이해하는 것은 하천복원에서 중요한 요소가 된다. 본 연구에서는 하천식생 복원모형에서의 홍수위 분석과 하상변동을 예측하고자 한다. 이를 위해 공주대교 상 하류 구간에 HEC-RAS와 RMA-2 모형을 적용하여 홍수위 분석을 실시함과 동시에 SED-2D 모형을 적용하여 하상변동을 예측하였다.

• 한국환경보건학회지
• /
• 제26권4호
• /
• pp.97-104
• /
• 2000

The purpose of this is made an examination of phosphorus loading model for eutrophication response in the Yongsan lake. For the model, we measured the total amount of nutrients derived from the Yongsan river watershed, inflow rate to the Yongsan lake, water quality, and water budget from January to December in 1999. The total amount of precipitation in the Yongsan river watershed was 4,951.7$\times$10$^{6}$ ㎥/y and inflow amount was 2,569.7$\times$10$^{6}$ ㎥/y, therefore the outflow rate of the Yongsan river watershed was 51.9%. The develop loading of total nitrogen was 86,928.1kg/d and that of total phosphorus was 22,007.6kg/d at the Yongsan river watershed, But, as the inflow loading of total nitrogen was 33,962kg/d and the inflow loading of total phosphorus was 2,218kg/d to the Yongsan lake. so each infolw rate was 39.0% and 10.1%. The hydraulic residence time was 34days, total phosphorus loading [L(P)] on the surface area was 23.398g/㎥/y, the hydraulic load( $Q_{s}$) of inflow water was 74.269m/y, the reserve rate of phosphorus in the lake was 0.359, and the settinh velocity of phosphorus was 0.114m/d at the Yongsan lake. Mathematical model of phosphorus loading to estimate the responses of eutrophication at the Yongsan lake is [ $P_{j}$] = 0.838 [L(P)/Q.(1+√ $T_{w}$)$^{-1}$ ] . ] . .

• 한국산학기술학회논문지
• /
• 제16권2호
• /
• pp.1441-1449
• /
• 2015

하천 만곡부에서는 높은 에너지의 편향적 흐름으로 인하여 제방침식 및 유사이송으로 하도변동을 야기하고 있는데 이러한 현상들은 제방의 불안정을 초래하고 인접 도로와 토지, 하천변 구조물에 심각한 문제를 야기할 수 있다. 본 연구에서는 제방 침식과 하도변동을 방지할 수 있는 Riprap Weir를 S자 만곡수로에 설치하고, 만곡부에서의 수리학적 흐름특성을 분석하고자 한다. 이를 위해 이차원 CCHE2D 모형을 이용하여 실험하도를 구축하고 Riprap Weir의 유무에 따른 수리특성분석을 실시하였다. 이를 통해 Riprap Weir의 설치간격에 따른 만곡부 내측과 외측에서의 수리특성과 보 인근의 흐름특성을 분석하였다.

• 한국물환경학회지
• /
• 제37권3호
• /
• pp.217-228
• /
• 2021

This paper discusses a methodology where an integrated modelling framework is used to quantify the risk derived from anthropic activities on habitats and species. To achieve this purpose, a tool comprising the Delft3D and HABITAT model, was applied in the Yeongsan river. Delft3D effectively simulated the operational condition and flow of weirs in river. In accuracy evaluation of the Delft3D-FLOW, the Bias, Pbias, Mean Absolute Error (MAE), Nash-Sutcliffe Efficiency (NSE), and Index of Agreement (IOA) were used, and the result was evaluated as grade above 'Satisfactory'. The HABITAT calculated Habitat Suitability Value (HSV) for the following eight species: mammal, fish, aquatic plant, and benthic macroinvertebrate. An Area was defined as a suitable habitat if the HSV was larger than 0.5. HABITAT was judged accurately by measuring the Correct Classification rate (CCR) and the area under the ROC curve (AUC). For benthic macroinvertebrate, the CCR and AUC were 77% and 0.834, respectively, at thresholds of 0.017 and 4 inds/m² for HSV and individuals per unit area. This meant that the HABITAT model accurately predicted the appearance of the benthic macroinvertebrates by approximately 77% and that the probability of false alarms was also very low. As a result of evaluating the suitability of habitats, in the Yeongsan river, if the annual "lowest level" (Seungchon weir: 2.5 EL.m/ Juksan weir: -1.35 EL.m) was maintained, the average habitat improvement effect of 6.5%P compared to the 'reference' scenario was predicted. Consequently, it was demonstrated that the integrated modelling framework for habitat suitability assessment is able to support the remedy aquatic ecological management.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2015년도 학술발표회
• /
• pp.211-211
• /
• 2015

Mekong River is the world's $10^{th}$ longest river and runs through China's Yunnan province, Burma, Thailand, Laos, Cambodia and Vietnam. And Tonle Sap Lake, the largest fresh water body in Southeast Asia and the heart of Mekong River system, covers an area $2,500-3,000Km^2$ in dry season and $10,000-16,000Km^2$ in wet season. As previously noted, the water within Sap river flows from the Mekong River to Tonle Sap Lake in flood season (between June and October) and backward to Mekong River in dry season. Recently the flow regime of Sap River might be significantly affected by the development of large dams in upstream region of Mekong River. This paper aims at basic study about the large scale flood inundation of Cambodia using by CAESAR-Lisflood. CAESAR-Lisflood is a geomorphologic / Landscape evolution model that combines the Lisflood-FP 2d hydrodynamic flow model (Bates et al, 2010) with the CAESAR geomorphic model to simulate flow hydrograph and erosion/deposition in river catchments and reaches over time scales from hours to 1000's of years. This model is based on the simplified full Saint-Venant Equation so that it can simulate the interacted flow of between Mekong River and Tonle Sap Lake especially focusing on the flow direction change of Sap River by season.

• Journal of Magnetics
• /
• 제21권3호
• /
• pp.431-436
• /
• 2016

In the design of transformer winding, natural vibration frequency is an important parameter. This paper presents a 2D model to calculate axial vibration natural frequency of power transformer winding based on the elastic dynamics theory, and according to the elastic support equivalent principle of radial pressboards. The 3D model to calculate natural vibration frequency can be simplified as a 2D one as the support of pressboards on the winding is same. It is verified that results of the 2D model are consistent with those of 3D one, but the former can achieve much higher calculation efficiency. It shows that increasing the width and number of pressboards can improve axial natural frequency through formula analysis and simulation, and also the relations between the changes of axial pre-compression and axial natural vibration frequency on the windings are investigated. Finally, the proposed 2D model's effectiveness is proved when compared with tested ones.