• BMB Reports
• /
• 제51권10호
• /
• pp.484-485
• /
• 2018

Receptor-interacting protein kinase-3 (RIP3 or RIPK3) is a serine-threonine kinase largely essential for necroptotic cell death; it also plays a role in some inflammatory diseases. High levels of RIP3 are likely sufficient to activate necroptotic and inflammatory pathways downstream of RIP3 in the absence of an upstream stimulus. For example, we have previously detected high levels or RIP3 in the skin of Toxic Epidermal Necrolysis patients; this correlates with increased phosphorylation of MLKL found in these patients. We have long surmised that there are molecular mechanisms to prevent anomalous activity of the RIP3 protein, and so prevent undesirable cell death and inflammatory effects when inappropriately activated. Recent discovery that Carboxyl terminus of Hsp 70-Interacting Protein (CHIP) could mediate ubiquitylation- and lysosome-dependent RIP3 degradation provides a potential protein that has this capacity. However, while screening for RIP3-binding proteins, we discovered that pellino E3 ubiquitin protein ligase 1 (PELI1) also interacts directly with RIP3 protein; further investigation in this study revealed that PELI1 also targets RIP3 for proteasome-dependent degradation. Interestingly, unlike CHIP, which targets RIP3 more generally, PELI1 preferentially targets kinase active RIP3 that has been phosphorylated on T182, subsequently leading to RIP3 degradation.

• BMB Reports
• /
• 제42권1호
• /
• pp.22-27
• /
• 2009

Replication Protein A (RPA) is a single stranded DNA-binding protein involved in DNA metabolic activities such as replication, repair, and recombination. RPA-Interacting Protein $\alpha$ ($RIP{\alpha}$) was originally identified as a nuclear transporter of RPA in Xenopus. The human $RIP{\alpha}$ gene encodes several splice isoforms, of which $hRIP{\alpha}$ and $hRIP{\beta}$ are the major translation products in vivo. However, limited information is available about the alternative splicing of $RIP{\alpha}$ in eukaryotes, apart from that in humans. In this study, we examined the alternative splicing of RIP{\alpha} in the Drosophila, Xenopus, and mouse system. We showed that the number of splice isoforms of RIP{\alpha} was species-specific, and displayed a tendency to increase in higher eukaryotes. Moreover, a mouse ortholog of $hRIP{\alpha}$, $mRIP{\beta}2$, was not SUMOylated, in contrast to $hRIP{\alpha}$. Based on these results, we suggest that the $RIP{\alpha}$ gene gains more splice isoforms and additional modifications after molecular evolution.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권12호
• /
• pp.7069-7074
• /
• 2013

Programmed cell death is a basic cellular process that is critical to maintaining tissue homeostasis. In contrast to apoptosis, necrosis was previously regarded as an unregulated and uncontrollable process. However, as research has progressed, necrosis, also known as necroptosis or programmed necrosis, is drawing increasing attention, not least becasu of its possible impications for cancer research. Necroptosis exhibits a unique signaling pathway that requires the involvement of receptor interaction protein kinases 1 and 3 (RIP1 and RIP3), mixed lineage kinase domain-like (MLKL), and phosphoglycerate mutase 5 (PGAM5) and can be specifically inhibited by necrostatins. Not only does necroptosis serve as a backup cell death program when apoptosis is inhibited, but it is now recognized to play a pivotal role in regulating various physiological processes and the pathogenesis of a variety of human diseases such as ischemic brain injury, immune system disorders and cancer. The control of necroptosis by various defined trigger factors and signaling pathways now offers the opportunity to target this cellular process for therapeutic purposes. The purpose of this paper is to review current findings concerning the connections between various trigger factors and the RIP1/RIP3 signaling pathway as it relates to necroptosis.

• Journal of Korean Biological Nursing Science
• /
• 제8권2호
• /
• pp.41-59
• /
• 2006

Chronic liver diseases and hepatic cancer have been reported as 10% of cause of death in Koreans. Regardless of various causes, chronic liver disease accompanies commonly hepatic fibrosis. But still the mechanism of hepatic fibrosis remains poorly understood. Using the dimethylnitrosamine(DMN)-induced hepatic fibrosis rat model, We performed to evaluate the possible therapeutic effect of RIP(extracts of Phellodendron amurense and Patrinia scabiosaefolia) and to investigate the changes in referential connective tissue proteins($TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin, and vimentin) as a marker of fibrogenesis. For these purposes, liver tissues were stained with H & E, and Azan staining for estimation of developing fibrosis. In the DMN-treated rat liver tissue, fibrosis were developed forming incomplete septal fibrosis. Whereas, in the RIP-treated rat liver tissues, the fibrosis were decreased recovering to normal morphology. The expressions of $TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin($\alpha-SMA$), and vimetin were increased in the DMN-treated rat liver tissues, but decreased in the various areas of RIP-treated rat liver tissues. According to these results, RIP could be a possible therapeutic agent to reduce hepatic fibrosis, and the $TGF-{\beta}_1$, ${\alpha}$-SMA, and vimentin could be possible indicative markers of hepatic fibrosis development and recovery.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권4호
• /
• pp.2277-2284
• /
• 2016

Ribosome-inactivating protein (RIP) from Mirabilis jalapa L. leaves has cytotoxic effects on breast cancer cell lines but is less toxic towards normal cells. However, it can easily be degraded after administration so it needs to be formulated into nanoparticles to increase its resistance to enzymatic degradation. The objectives of this study were to develop a protein extract of M. jalapa L. leaves (RIP-MJ) incorporated into nanoparticles conjugated with Anti-EpCAM antibodies, and to determine its cytotoxicity and selectivity in the T47D breast cancer cell line. RIP-MJ was extracted from red-flowered M. jalapa L. leaves. Nanoparticles were formulated based on polyelectrolyte complexation using low viscosity chitosan and alginate, then chemically conjugated with anti-EpCAM antibody using EDAC based on carbodiimide reaction. RIP-MJ nanoparticles were characterised for the particle size, polydispersity index, zeta potential, particle morphology, and entrapment efficiency. The cytotoxicity of RIP-MJ nanoparticles against T47D and Vero cells was then determined with MTT assay. The optimal formula of RIP-MJ nanoparticles was obtained at the concentration of RIP-MJ, low viscosity chitosan and alginate respectively 0.05%, 1%, and 0.4% (m/v). RIP-MJ nanoparticles are hexagonal with high entrapment efficiency of 98.6%, average size of 130.7 nm, polydispersity index of 0.380 and zeta potential +26.33 mV. The $IC_{50}$ values of both anti-EpCAM-conjugated and non-conjugated RIP-MJ nanoparticles for T47D cells (13.3 and $14.9{\mu}g/mL$) were lower than for Vero cells (27.8 and $33.6{\mu}g/mL$). The $IC_{50}$ values of conjugated and non-conjugated RIP-MJ for both cells were much lower than $IC_{50}$ values of non-formulated RIP-MJ (>$500{\mu}g/mL$).

• 한국정보통신학회논문지
• /
• 제6권1호
• /
• pp.36-40
• /
• 2002

Routing algorithm uses metric to choose the route of Least cost to destination network, the best suited routing investigates all routes to the shortest destination among networks and is decided on the route given the minimum metric. This paper analyzed packet flow for setting up the best fitted path on the same network using RIP and EIGRP as the distance vector algorithm and measured the Link-efficiency.

• 한국정보통신학회논문지
• /
• 제6권1호
• /
• pp.30-35
• /
• 2002

동적 라우팅은 최적경로를 선택하고 라우팅테이블을 업데이트 하기 위해 라우팅 프로토콜을 사용한다. 가장 널리 사용되는 라우팅 프로토콜은 거리벡터 알고리즘을 이용한 라우팅인포메이션 프로토콜(RIP)이다. RIP는 최적경로서 최저 흡수의 경로를 취한다. 하지만 이 RIP는 매우 심각한 단점을 가지고 있다. 그것은 15 흡수 이상의 목적지에 대한 네트워크의 라우팅테이블을 유지할 수 없다는 것이다. 이를 극복하기 위해 TCP/IP에서 개발된 링크상태 프로토콜인 OSPF가 사용된다. OSPF는 큰 네트워크에 적합하고 RIP가 갖은 단점들을 극복 했다. 본 논문은 동일한 네트워크에서 메세지 전달과 지연, 링크 사용율, 메세지 전달갯수 같은 두 프로토콜사이의 트래픽과 링크효율을 분석하였다.

• 한국해안·해양공학회논문집
• /
• 제31권6호
• /
• pp.485-492
• /
• 2019

본 연구진에서 개발한 항해용 X-band 레이다를 이용한 파랑 및 이안류 관측 시스템을 해운대 해수욕장에 설치하여 이안류를 관측하였다. 기존 이안류 현장관측 방법으로는 측정하기 어려운 이안류의 형태와 크기 및 유속을 X-band 레이다 영상분석을 통해 관측하였다. 본 연구에서는 시간평균한 X-band 레이다 영상으로부터 이안류 발생위치와 크기 및 형태를 자동탐지하는 알고리즘을 개발하였다. X-band 레이다 영상을 3차원 푸리에 변환하고, 도플러 유속이 포함된 분산방정식 조건으로 이안류 유속을 계산하였다. X-band 레이다 영상분석을 통한 이안류 발생위치와 유속 계산 기법은 표류부이 실험으로 검증하였다. 해운대 해수욕장 이안류 조사 결과 한번 발생한 이안류는 1~2일 동안 지속되며, 발생위치가 크게 바뀌지 않는 것이 관찰되었다.

• 지역사회간호학회지
• /
• 제30권4호
• /
• pp.460-470
• /
• 2019

Purpose: This study was to identify the factors influencing the practice of respiratory infection prevention (RIP) for the elderly residing in rural areas. Methods: The data were collected from 188 residents aged 65 years or older residing in the jurisdictions of four public health clinics in rural areas of C city, using a structured questionnaire for the period from December 1, 2018 to February 28, 2019. The collected data were analyzed with independent t-test, one-way ANOVA, Pearson's correlation coefficients, and hierarchical multiple regression analysis using SPSS/WIN 25.0 program. Results: The score on knowledge of RIP was 8.82±1.36, while it was 4.53±0.47 for attitude, 3.78±0.66 for practice, and 3.78±0.69 for social capital. It was observed that the factors influencing the practice of RIP were attitude of RIP (β=.38, p<.001), social capital (β=.29, p<.001), family type (β=-.19, p=.002), and subjective health status (β=.15, p=.035), while the explanatory power of the model was 47%. Conclusion: Consequently, to enhance the practice of RIP for the elderly residing in rural areas, it is necessary to develop programs considering social and environmental characteristics of rural areas based on their attitude of RIP, social capital, family type and subjective health status.

• 한국구조물진단유지관리공학회 논문집
• /
• 제6권4호
• /
• pp.157-167
• /
• 2002

In most cases, quantity of reinforcement is determined without regard to the difference of initial strain, and status of damages when calculated the strengthening in flexure at beams. Thus, the purpose of this study is to investigate the flexural strengthening efficiency and behavior of RC beams strengthened with carbon fiber sheets(CFS) considering different status of damages. in this paper, a nonlinear analysis program considering rip-off strength and residual stress of steel bars and concrete in different status of damages is developed to predict the flexural behavior of CFS strengthened beams. Rip-off strength equation is obtained by modifying moment of inertia in the Robert's equation. And conformed developed nonlinear analysis program in variable of strengthening CFS amount and status of damages(initial, case1, case2, case3) and tension reinforcement ratio(0.2~1.0%).