• BMB Reports
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• 제51권10호
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• pp.484-485
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• 2018

Receptor-interacting protein kinase-3 (RIP3 or RIPK3) is a serine-threonine kinase largely essential for necroptotic cell death; it also plays a role in some inflammatory diseases. High levels of RIP3 are likely sufficient to activate necroptotic and inflammatory pathways downstream of RIP3 in the absence of an upstream stimulus. For example, we have previously detected high levels or RIP3 in the skin of Toxic Epidermal Necrolysis patients; this correlates with increased phosphorylation of MLKL found in these patients. We have long surmised that there are molecular mechanisms to prevent anomalous activity of the RIP3 protein, and so prevent undesirable cell death and inflammatory effects when inappropriately activated. Recent discovery that Carboxyl terminus of Hsp 70-Interacting Protein (CHIP) could mediate ubiquitylation- and lysosome-dependent RIP3 degradation provides a potential protein that has this capacity. However, while screening for RIP3-binding proteins, we discovered that pellino E3 ubiquitin protein ligase 1 (PELI1) also interacts directly with RIP3 protein; further investigation in this study revealed that PELI1 also targets RIP3 for proteasome-dependent degradation. Interestingly, unlike CHIP, which targets RIP3 more generally, PELI1 preferentially targets kinase active RIP3 that has been phosphorylated on T182, subsequently leading to RIP3 degradation.

• 한국수자원학회논문집
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• 제56권8호
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• pp.521-528
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• 2023

FUNWAVE 이안류 수치모의를 통해 얻은 이안류 발생정도 분포(파고-주기, 파고-조위, 파고-파향, 파고-주파수 스펙트럼 광협도, 파고-파향 스펙트럼 광협도)를 이용한 이안류 위험지수 생산 기법을 이안류 예보체계에 적용하였다. 이 기법은 관측 기반의 실시간 이안류 경보체계를 위해 개발되었으나, 관측정보를 대신하여 파랑예보 정보를 사용하므로 예보체계에 활용하였다. 2021년 해운대 해수욕장에 대하여 관측 기반으로 생산된 이안류 위험지수와 파랑예보 기반의 지수를 비교하여 그 가능성을 검토하였다.

• BMB Reports
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• 제42권1호
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• pp.22-27
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• 2009

Replication Protein A (RPA) is a single stranded DNA-binding protein involved in DNA metabolic activities such as replication, repair, and recombination. RPA-Interacting Protein $\alpha$ ($RIP{\alpha}$) was originally identified as a nuclear transporter of RPA in Xenopus. The human $RIP{\alpha}$ gene encodes several splice isoforms, of which $hRIP{\alpha}$ and $hRIP{\beta}$ are the major translation products in vivo. However, limited information is available about the alternative splicing of $RIP{\alpha}$ in eukaryotes, apart from that in humans. In this study, we examined the alternative splicing of RIP{\alpha} in the Drosophila, Xenopus, and mouse system. We showed that the number of splice isoforms of RIP{\alpha} was species-specific, and displayed a tendency to increase in higher eukaryotes. Moreover, a mouse ortholog of $hRIP{\alpha}$, $mRIP{\beta}2$, was not SUMOylated, in contrast to $hRIP{\alpha}$. Based on these results, we suggest that the $RIP{\alpha}$ gene gains more splice isoforms and additional modifications after molecular evolution.

• 한국항해항만학회지
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• 제41권2호
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• pp.55-62
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• 2017

A nearshore current or a wave-induced current is an important phenomenon in a nearshore zone, which is composed of longshore, cross-shore, and rip currents. The nearshore current is closely related to the occurrence of coastal accidents by beachgoers. A considerable number of coastal accidents by beachgoers involving the rip current have been reported at Jungmun Beach. However, in studies and observations of the nearshore current of Jungmun Beach, understanding of the rip current pattern remains unclear. In this study, a scientific approach is taken to understand the nearshore current and the rip current patterns at Jungmun Beach by numerical computation for year of 2015. From results of numerical computation, the occurrence and spatial characteristics of the rip current, and the similarities between the rip current and incident wave conditions are analyzed. The primary results of this study reveal that the rip currents are frequently generated at Jungmun Beach, especially in the western parts of the beach, and that the rip currents often occur with a wave breaking height of around 0.5 ~ 0.7 m, a wave period of around 6 ~ 8 seconds, and a breaking angle of around 0 ~ 15 degrees.

• Nuclear Engineering and Technology
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• 제31권3호
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• pp.257-266
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• 1999

A simplified statistical methodology is developed in order to both reduce over-conservatism of deterministic methodologies employed for PWR fuel rod internal pressure (RIP) calculation and simplify the complicated calculation procedure of the widely used statistical methodology which employs the response surface method and Monte Carlo simulation. The simplified statistical methodology employs the system moment method with a deterministic approach in determining the maximum variance of RIP The maximum RIP variance is determined with the square sum of each maximum value of a mean RIP value times a RIP sensitivity factor for all input variables considered. This approach makes this simplified statistical methodology much more efficient in the routine reload core design analysis since it eliminates the numerous calculations required for the power history-dependent RIP variance determination. This simplified statistical methodology is shown to be more conservative in generating RIP distribution than the widely used statistical methodology. Comparison of the significances of each input variable to RIP indicates that fission gas release model is the most significant input variable.

• Molecules and Cells
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• 제46권11호
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• pp.710-724
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• 2023

The plant defense responses to microbial infection are tightly regulated and integrated with the developmental program for optimal resources allocation. Notably, the defense-associated hormone salicylic acid (SA) acts as a promoter of flowering while several plant pathogens actively target the flowering signaling pathway to promote their virulence or dissemination. Ralstonia pseudosolanacearum inject tens of effectors in the host cells that collectively promote bacterial proliferation in plant tissues. Here, we characterized the function of the broadly conserved R. pseudosolanacearum effector RipL, through heterologous expression in Arabidopsis thaliana. RipL-expressing transgenic lines presented a delayed flowering, which correlated with a low expression of flowering regulator genes. Delayed flowering was also observed in Nicotiana benthamiana plants transiently expressing RipL. In parallel, RipL promoted plant susceptibility to virulent strains of Pseudomonas syringae in the effector-expressing lines or when delivered by the type III secretion system. Unexpectedly, SA accumulation and SA-dependent immune signaling were not significantly affected by RipL expression. Rather, the RNA-seq analysis of infected RipL-expressing lines revealed that the overall amplitude of the transcriptional response was dampened, suggesting that RipL could promote plant susceptibility in an SA-independent manner. Further elucidation of the molecular mechanisms underpinning RipL effect on flowering and immunity may reveal novel effector functions in host cells.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.2277-2284
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• 2016

Ribosome-inactivating protein (RIP) from Mirabilis jalapa L. leaves has cytotoxic effects on breast cancer cell lines but is less toxic towards normal cells. However, it can easily be degraded after administration so it needs to be formulated into nanoparticles to increase its resistance to enzymatic degradation. The objectives of this study were to develop a protein extract of M. jalapa L. leaves (RIP-MJ) incorporated into nanoparticles conjugated with Anti-EpCAM antibodies, and to determine its cytotoxicity and selectivity in the T47D breast cancer cell line. RIP-MJ was extracted from red-flowered M. jalapa L. leaves. Nanoparticles were formulated based on polyelectrolyte complexation using low viscosity chitosan and alginate, then chemically conjugated with anti-EpCAM antibody using EDAC based on carbodiimide reaction. RIP-MJ nanoparticles were characterised for the particle size, polydispersity index, zeta potential, particle morphology, and entrapment efficiency. The cytotoxicity of RIP-MJ nanoparticles against T47D and Vero cells was then determined with MTT assay. The optimal formula of RIP-MJ nanoparticles was obtained at the concentration of RIP-MJ, low viscosity chitosan and alginate respectively 0.05%, 1%, and 0.4% (m/v). RIP-MJ nanoparticles are hexagonal with high entrapment efficiency of 98.6%, average size of 130.7 nm, polydispersity index of 0.380 and zeta potential +26.33 mV. The $IC_{50}$ values of both anti-EpCAM-conjugated and non-conjugated RIP-MJ nanoparticles for T47D cells (13.3 and $14.9{\mu}g/mL$) were lower than for Vero cells (27.8 and $33.6{\mu}g/mL$). The $IC_{50}$ values of conjugated and non-conjugated RIP-MJ for both cells were much lower than $IC_{50}$ values of non-formulated RIP-MJ (>$500{\mu}g/mL$).

• 대한수학회보
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• 제50권6호
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• pp.1957-1972
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• 2013

The reflexive property for ideals was introduced by Mason and has important roles in noncommutative ring theory. In this note we study the structure of idempotents satisfying the reflexive property and introduce reflexive-idempotents-property (simply, RIP) as a generalization. It is proved that the RIP can go up to polynomial rings, power series rings, and Dorroh extensions. The structure of non-Abelian RIP rings of minimal order (with or without identity) is completely investigated.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7069-7074
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• 2013

Programmed cell death is a basic cellular process that is critical to maintaining tissue homeostasis. In contrast to apoptosis, necrosis was previously regarded as an unregulated and uncontrollable process. However, as research has progressed, necrosis, also known as necroptosis or programmed necrosis, is drawing increasing attention, not least becasu of its possible impications for cancer research. Necroptosis exhibits a unique signaling pathway that requires the involvement of receptor interaction protein kinases 1 and 3 (RIP1 and RIP3), mixed lineage kinase domain-like (MLKL), and phosphoglycerate mutase 5 (PGAM5) and can be specifically inhibited by necrostatins. Not only does necroptosis serve as a backup cell death program when apoptosis is inhibited, but it is now recognized to play a pivotal role in regulating various physiological processes and the pathogenesis of a variety of human diseases such as ischemic brain injury, immune system disorders and cancer. The control of necroptosis by various defined trigger factors and signaling pathways now offers the opportunity to target this cellular process for therapeutic purposes. The purpose of this paper is to review current findings concerning the connections between various trigger factors and the RIP1/RIP3 signaling pathway as it relates to necroptosis.

• 한국수자원학회논문집
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• 제55권9호
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• pp.645-655
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• 2022

최근 동해안 해수욕장에서도 여름철 해수욕 기간에 이안류가 자주 관찰되고 있다. 우리나라 동해안에 위치한 해수욕장들에서 발생하는 이안류의 원인 특성을 수치모의 결과를 이용하여 검토하였다. 속초, 낙산, 경포대, 망상 해수욕장 앞바다의 수심자료를 이용하여 Boussinesq 방정식을 지배 방정식으로 하는 FUNWAVE 모형으로 수치모의를 수행하였다. 각 해수욕장 앞바다 지형, 샌드바 형성 및 입사파 특성 등의 영향으로 변형되는 위상의 상호작용을 포함한 파의 횡방향 비균등성에 의해 발생하는 이안류가 잘 재현되었다. 그 결과를 기반으로 각 해수욕장에서 발생 가능한 이안류의 종류를 기술하였다.