• 제목/요약/키워드: REB

검색결과 20건 처리시간 0.066초

미생물 생장 예측모델과 반응표면분석법을 이용한 Vibrio parahaemolyticus의 신속 증균배지 개발 (Development of a Rapid Enrichment Broth for Vibrio parahaemolyticus Using a Predictive Model of Microbial Growth with Response Surface Analysis)

  • 서연희;이소영;김은지;오세욱
    • 한국식품위생안전성학회지
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    • 제38권6호
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    • pp.449-456
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    • 2023
  • 본 논문은 적은 농도로 존재하는 Vibrio parahaemolyticus를 6시간이내에 106 CFU/mL까지 신속하게 배양가능한 배지인 Rapid Enrichment Broth for V. parahaemolyticus(REB-V)를 개발하여 V. parahaemolyticus를 신속하게 검출할 수 있도록 하였다. Modified Gompertz model과 RSM를 활용하여 V. parahaemolyticus를 신속하게 증균할 수 있도록 첨가 성분을 최적화하였다. 그 결과 2%(w/v) NaCl BPW에 D-(+)-mannose 0.3 g/L, L-valine 0.2 g/L, magnesium sulfate 0.2 g/L를 첨가하였을 때 V. parahaemolyticus의 증균량이 최대였다. REB-V의 배양조건을 pH 7.84와 37℃으로 최적화하였으며, 2%(w/v) NaCl BPW와 비교하여 REB-V의 증균 효율을 확인하기 위해 7시간 증균을 통해 증균량을 평가하였다. 또한 매시간마다 배양액에서 DNA를 추출하였고, 추출한 DNA로 real-time PCR을 수행하여 REB-V의 분자진단법 적용가능성을 확인하였다. 그 결과 7시간 동안 2%(w/v) NaCl BPW에서 V. parahaemolyticus는 5.452±0.151 Log CFU/mL까지 증균되었고, REB-V에서는 7.831±0.323 Log CFU/mL의 V. parahaemolyticus가 증균되었다. 최종적으로 REB-V에서 6시간 이내에 106 CFU/mL 이상으로 증균된 것을 확인하였고, REB-V의 증균속도가 2%(w/v) NaCl BPW보다 빠르고 같은 시간 내의 증균량이 2%(w/v) NaCl BPW보다 많았음을 통해 REB-V의 증균효율이 우수한 것으로 확인되었다.

격자형 탄소섬유강화플라스틱의 외부부착 보강에 따른 철근 콘크리트 보의 구조적 거동에 관한 실험적 연구 (An Experimental Study on the Structural Behavior of Reinforced Concrete Beam with External Adhesion of CFRP Grid)

  • 김상우;김건우;김진섭
    • 토지주택연구
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    • 제12권4호
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    • pp.119-125
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    • 2021
  • 본 연구에서는 그리드를 이용한 철근콘크리트 휨부재의 연성보강 효과를 실험결과로 제시했다. 실험변수로는 비보강 철근콘크리트 휨부재(ORI), 바닥면 보강 철근콘크리트 휨부재(REB), 바닥 및 측면 보강 철근콘크리트 휨부재(REBS)가 있다. 실험은 4절점 휨 실험으로 수행되었다. 실험 결과, 보강을 통해 최대 휨강도가 17~20% 증가하였고, 측면 보강을 통해 휨 파괴 유도를 통한 연성보강 효과를 확인하였다. 또한, 연성지수를 계산하여 REB와 REBS의 연성지수가 ORI 대비 각각 2배, 3배 증가하였음을 확인하였다.

대학생들의 통제성향과 환경행동에 관한 연구 (A Study on the Locus of Control and Environmental Behavior of Undergraduate Students)

  • 오해섭
    • 농촌지도와개발
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    • 제3권2호
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    • pp.265-271
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    • 1996
  • Locals of Control is considered one of the main imporment factors on responsible environmental behaviors(REB). It is defined as an individual's perception of his or ha ability to bring about change through his a her behavior. This psychological construct is divided in two, external and internal. Internal locus of control(ILOC) is imporment as predictor for responsible environmental behaviors. This study was conducted to investigate the Locus of Control and relations with REB in Undergraduate Students. The data were collected mainly through questionnaires and a total of 157 responded questionnaires was analyzed for the study. The statistical methods used in this study were frequency, percentile and correlation. The major findings of this study were (1) Most of undergraduate students had internal locus of control(75.2%). (2) According to region, rural students showed high levels in ILOC and REB.

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헝거포드 접근법의 행동주의를 넘어서 (Beyond the Behaviorism Embedded in the Hungerford Approach)

  • 이재영
    • 한국환경교육학회지:환경교육
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    • 제15권1호
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    • pp.68-82
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    • 2002
  • My responses to Kim Kyung-Ok's Critique on my critique on the Hungerford approach can be summarized as follows; First, it was argued that possible confusions and misunderstandings around the concept of behavior in REB were mainly caused by Hungerford himself who has used the word in several different ways, from a bunch of overt actions to almost all kinds of responses including cognitive skills, without any clear operational definition of it for more than 20 years. It seems to be needed for future users of the word, 'Behavior' to Prevent unnecessary confusions by providing their operational definition of it. Second, REB is too ambiguous to be a legitimate goal of environmental education and too outcome-oriented to be a meaningful measure for environmental education research. Anyone who accept REB as a goal of EE or a measure for research should clearly suggest procedures and criteria for judging the environmental responsibility of actions under consideration. Third, the Hungerford approach has begun by realizing the limit of a linear traditional behavior change system and has been evolving toward a complex model with dynamic interactions among/between cognitive variables and affective variables. However, it still has one-way structural orientation toward 'Behavior' with no feedbacks. Addition of some feedback processes would make the model more flexible and realistic. Finally, both the Hines model and the Hungeford model were established based on a series of behavioristic studies including three doctoral dissertations equiped with a list of actions which were prejudged to be environmentally responsible by the researchers, not by the learners. What they were primarily interested in was not how mind functions during the learning processes but how learners' behavior can be effectively changed. Considering uncertainty and complexity associated with environmental problems, a great deal of efforts ought to be made toward more context-based and less normative studies applying cognitive psychology and quantitative approaches.

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고등학생들의 성별 조절점 방향 실태 및 환경 행동과의 관련성 조사 (Orientation of Locus of Control and Environmental Behavior in High School Students)

  • 박진희;장남기
    • 한국환경교육학회지:환경교육
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    • 제9권1호
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    • pp.17-29
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    • 1996
  • 'Control of Locus of Reinforcement(LOCR)' is considered one of the main important factors on responsible environmental behaviors(REB). It is defined as 'an individual's perception of his or her ability to bring about change through his or her behavior'. This phychological construct is divided in two, external locus and internal locus. Internal locus of reinforcement is important as predictor for REB. Lately, 'The Environmental Action Internal Control Index:EAICI' was developed. It is a valid and reliable instrument to measure relationships of two variables. The purposes of this study were to analyse the orientation of LOCR in high school students and relations with LOCR to REB. By the results, EAICI scores of total students, males, females are 99.83, 95.10, 104.56, respectively. LOCR of females was stronger and more internal than one of males. The item scores for behaviors that reduce the amount of household trash by reusing and recycling and convince someone to do this are 4.31, 4.05, respectively. The item scores for behaviors that convince someone to sign a petition regarding on environmental issues, convince someone to reuse envelopes by putting a label over the old address and convince someone to keep car tires properly inflated are 3.09, 3.09, 3.04, respectively. It shows that EAICI scores are dependent upon the degree of the chances and experiences to meet the various environmental events.

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차량안전 응용을 위한 임의 조기 비컨방송 (Random Early Beacon Broadcast (REB2) for Cooperative Vehicle Safety Applications)

  • 정한유;응웬 호아 흥
    • 전기전자학회논문지
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    • 제24권4호
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    • pp.1017-1021
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    • 2020
  • V2X 통신에서 차량들은 비컨(Beacon)이라고 불리는 메시지의 주기적 방송을 통해 주변 차량들이 송신 차량의 이동상태를 정확하게 예측할 수 있도록 지원한다. 특히, 이동 적응형 비컨 방송 기술(MAB2)은 일정한 한도 내에서 연속된 비컨 손실이 발생하더라도 송신차량의 추적 오차를 임계치 이하로 유지할 수 있는 비컨방송기법이다. 본 논문에서는 MAB2 기술이 야기할 수 있는 비컨의 노후화 문제를 해결하기 위한 임의 조기 비컨 방송 기법을 제시하고, 시뮬레이션을 통해 충돌사고가 발생할 수 있는 100 m 이내 근거리 차량의 추적실패확률이 1/10 이하로 감소함을 보인다.

자아 존중감이 환경 행동에 미치는 영향 고찰과 고등학생의 자아 존중감에 관한 실태 조사 (Investigation into the Relationship between Self-Esteem and Environmental Behavior and Research into Self-Esteem in High School Students)

  • 박진희;장남기
    • 한국환경교육학회지:환경교육
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    • 제10권2호
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    • pp.63-74
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    • 1997
  • 'Self-esteem' is defined as 'the lived status of one's individual competence and personal worthiness in dealing with the challenges of Life over Time' and high self-esteem is associated with self-confidence, effectively coping, well-being, and responsibility. The first purpose of this study was to identify the concept of self-esteem and to investigate the relationship with self-esteem and responsible environmental behaviors (REB). According to the results, it was found that 'self-esteem' is considered as one of the influential factors on REB and self-esteem enhancement is essential for the responsible choice and determination of environments. The second purpose was to analyse the level of self-esteem in High School students. The mean scores of merits and demerits for all the students involved (402) were 14.58 and 14.91 respectively. By applying the t-test, the two scores were not so different: thus the mean of self-esteem was found to be at a median level. The number of adove-average, average and below-average levels were 114, 163 and 125 respectively. From now on, the development of strategies to enhance self-esteem is urgently needed.

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Radial Electrical Impedance: A Potential Indicator for Noninvasive Cuffless Blood Pressure Measurement

  • Huynh, Toan Huu;Chung, Wan-Young
    • 센서학회지
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    • 제26권4호
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    • pp.239-244
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    • 2017
  • Noninvasive, cuffless, and continuous blood pressure (BP) monitoring is essential to prevent and control hypertension. A well-known existing method for this measurement is pulse transit time (PTT), which has been investigated by many researchers as a promising approach. However, the fundamental principle of the PTT method is based on the time interval taken by a pulse wave to propagate between the proximal and distal arterial sites. Consequently, this method needs an independent system with two devices placed at two different sites, which is a problem. Even though some studies attempted to synchronize the system, it is bulky and inconvenient by contemporary standards. To find a more sensitive method to be used in a BP measurement device, this study used radial electrical bioimpedance (REB) as a potential indicator for BP determination. Only one impedance plethysmography channel at the wrist is performed for demonstrating a ubiquitous BP wearable device. The experiment was evaluated on eight healthy subjects with the ambulatory BP monitor on the upper arm as a reference. The results demonstrated the potential of the proposed method by the correlation of estimated systolic (SBP) and diastolic (DBP) BP against the reference at $0.84{\pm}0.05$ and $0.83{\pm}0.05$, respectively. REB also tracked the DBP well with a root-mean-squared-error of $7.5{\pm}1.35mmHg$.

내적 조절점 강화를 위한 자기 조절 관리 전략의 효과 (The Effect of 'Self Control Management Strategy' on the Reinforcement of Internal Locus of Control)

  • 박진희;장남기
    • 한국과학교육학회지
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    • 제17권4호
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    • pp.347-358
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    • 1997
  • Locus of Control of Reinforcement (LOCR)' is considered as one of the most important factors on responsible environmental behaviors(REB). It is defined as 'an individual's perception of his or her ability to bring about change through his or her behavior'. This psychological construct is divided up into two directions: the external locus and internal locus. The internal locus is important as a predictor of REB. Recently, 'The Environmental Action Internal Control Index: EAICI' has been developed and it is a valid and reliable instrument to measure the relationship of two variables. The purposes of this study were to develop a strategy to reinforce the internal locus or to converse external locus to internal locus. and to verify the effects by EAICI. A new strategy, 'Self Control Management Strategy' was based on the characteristics of internal locus and the key idea of this was to self-control and to manage the courses by themselves. EAICI scores of the control group was 99.83. All of them were internal and the LOCR of females was more internal than that of the males. This strategy was applied when the instructions were carried out on seven environmental issues by the experimental group. According to the results. the seven points of the EAICI were increased significantly. Therefore this strategy is helpful in reinforcing the internal locus or to converse external locus to the internal locus.

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Severe Bilateral Microphthalmia in a Holstein Calf

  • Hur, Tai-Young;Kang, Seog-Jin;Jung, Young-Hun;Do, Yoon-Jung;Choi, Chang-Yong;Ryu, Il-Sun;Cho, Sung-Whan;Song, Kun-Ho;Suh, Guk-Hyun
    • 한국임상수의학회지
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    • 제25권5호
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    • pp.411-414
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    • 2008
  • This report described a severe bilateral microphthalmia in a young Holstein calf. The anomalous calf with wry tail showed normal vigor, appetite and normal body weight except for eye defect. The orbits were shallower and smaller than normal. The orbit bilaterally contained a white small mass suspected as eyeball. A spot-like remnant of eyeball (REB) was buried in the mixture of vestigial extraocular muscles and adipose tissue of the bilateral orbit. Histologically, the REB was composed of irregularly arranged elements of ocular wall such as sclera, retina and ciliary body. But any destructive changes in the central nervous systems were not detected. This case of eye defect was defined as severe bilateral microphthalmia. The cause of this ocular defect is unknown.