• Title/Summary/Keyword: REB

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Development of a Rapid Enrichment Broth for Vibrio parahaemolyticus Using a Predictive Model of Microbial Growth with Response Surface Analysis (미생물 생장 예측모델과 반응표면분석법을 이용한 Vibrio parahaemolyticus의 신속 증균배지 개발)

  • Yeon-Hee Seo;So-Young Lee;Unji Kim;Se-Wook Oh
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.449-456
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    • 2023
  • In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 100 CFU/mL to 106 CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 106 CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency.

An Experimental Study on the Structural Behavior of Reinforced Concrete Beam with External Adhesion of CFRP Grid (격자형 탄소섬유강화플라스틱의 외부부착 보강에 따른 철근 콘크리트 보의 구조적 거동에 관한 실험적 연구)

  • Kim, Sang-Woo;Kim, Geon-Woo;Kim, Jin-Sup
    • Land and Housing Review
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    • v.12 no.4
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    • pp.119-125
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    • 2021
  • The study presents the ductility reinforcement effect of the RC bending member using the CFRP Grid as an experimental result. Experimental variables include a non-reinforced RC bending member (ORI), a bottom reinforced RC bending member (REB), and an RC bending member reinforced at the bottom and side (REBS). The experiment was carried out with four points bending test. As a result of the experiment, it was confirmed that the maximum bending strength increased by 17-20% through reinforcement. In addition, the ductility index calculation results confirmed that the ductility index of REB and REBS increased by 2 and 3 times, respectively, compared to the ORI.

A Study on the Locus of Control and Environmental Behavior of Undergraduate Students (대학생들의 통제성향과 환경행동에 관한 연구)

  • Oh, Hae-Sub
    • Journal of Agricultural Extension & Community Development
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    • v.3 no.2
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    • pp.265-271
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    • 1996
  • Locals of Control is considered one of the main imporment factors on responsible environmental behaviors(REB). It is defined as an individual's perception of his or ha ability to bring about change through his a her behavior. This psychological construct is divided in two, external and internal. Internal locus of control(ILOC) is imporment as predictor for responsible environmental behaviors. This study was conducted to investigate the Locus of Control and relations with REB in Undergraduate Students. The data were collected mainly through questionnaires and a total of 157 responded questionnaires was analyzed for the study. The statistical methods used in this study were frequency, percentile and correlation. The major findings of this study were (1) Most of undergraduate students had internal locus of control(75.2%). (2) According to region, rural students showed high levels in ILOC and REB.

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Beyond the Behaviorism Embedded in the Hungerford Approach (헝거포드 접근법의 행동주의를 넘어서)

  • 이재영
    • Hwankyungkyoyuk
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    • v.15 no.1
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    • pp.68-82
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    • 2002
  • My responses to Kim Kyung-Ok's Critique on my critique on the Hungerford approach can be summarized as follows; First, it was argued that possible confusions and misunderstandings around the concept of behavior in REB were mainly caused by Hungerford himself who has used the word in several different ways, from a bunch of overt actions to almost all kinds of responses including cognitive skills, without any clear operational definition of it for more than 20 years. It seems to be needed for future users of the word, 'Behavior' to Prevent unnecessary confusions by providing their operational definition of it. Second, REB is too ambiguous to be a legitimate goal of environmental education and too outcome-oriented to be a meaningful measure for environmental education research. Anyone who accept REB as a goal of EE or a measure for research should clearly suggest procedures and criteria for judging the environmental responsibility of actions under consideration. Third, the Hungerford approach has begun by realizing the limit of a linear traditional behavior change system and has been evolving toward a complex model with dynamic interactions among/between cognitive variables and affective variables. However, it still has one-way structural orientation toward 'Behavior' with no feedbacks. Addition of some feedback processes would make the model more flexible and realistic. Finally, both the Hines model and the Hungeford model were established based on a series of behavioristic studies including three doctoral dissertations equiped with a list of actions which were prejudged to be environmentally responsible by the researchers, not by the learners. What they were primarily interested in was not how mind functions during the learning processes but how learners' behavior can be effectively changed. Considering uncertainty and complexity associated with environmental problems, a great deal of efforts ought to be made toward more context-based and less normative studies applying cognitive psychology and quantitative approaches.

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Orientation of Locus of Control and Environmental Behavior in High School Students (고등학생들의 성별 조절점 방향 실태 및 환경 행동과의 관련성 조사)

  • 박진희;장남기
    • Hwankyungkyoyuk
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    • v.9 no.1
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    • pp.17-29
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    • 1996
  • 'Control of Locus of Reinforcement(LOCR)' is considered one of the main important factors on responsible environmental behaviors(REB). It is defined as 'an individual's perception of his or her ability to bring about change through his or her behavior'. This phychological construct is divided in two, external locus and internal locus. Internal locus of reinforcement is important as predictor for REB. Lately, 'The Environmental Action Internal Control Index:EAICI' was developed. It is a valid and reliable instrument to measure relationships of two variables. The purposes of this study were to analyse the orientation of LOCR in high school students and relations with LOCR to REB. By the results, EAICI scores of total students, males, females are 99.83, 95.10, 104.56, respectively. LOCR of females was stronger and more internal than one of males. The item scores for behaviors that reduce the amount of household trash by reusing and recycling and convince someone to do this are 4.31, 4.05, respectively. The item scores for behaviors that convince someone to sign a petition regarding on environmental issues, convince someone to reuse envelopes by putting a label over the old address and convince someone to keep car tires properly inflated are 3.09, 3.09, 3.04, respectively. It shows that EAICI scores are dependent upon the degree of the chances and experiences to meet the various environmental events.

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Random Early Beacon Broadcast (REB2) for Cooperative Vehicle Safety Applications (차량안전 응용을 위한 임의 조기 비컨방송)

  • Jeong, Han-You;Nguyen, Hoa-Hung
    • Journal of IKEEE
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    • v.24 no.4
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    • pp.1017-1021
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    • 2020
  • In the V2X communications, each vehicle periodically broadcasts a message, called a beacon, so that its neighbor vehicles can accurately predict the near-future positions of the sending vehicle. In particular, the mobility-adaptive beacon broadcast (MAB2) scheme keeps the tracking error of a sending vehicle below a fixed threshold within a few consecutive beacon losses. In this paper, we present a random-early beacon broadcast (REB2) scheme that addresses the beacon aging problem of the MAB2 scheme. The simulation results show that, for a neighbor vehicle within 100 m distance, the tracking failure probability of REB2 scheme can be reduced to less than 1/10 of that of MAB2 scheme.

Investigation into the Relationship between Self-Esteem and Environmental Behavior and Research into Self-Esteem in High School Students (자아 존중감이 환경 행동에 미치는 영향 고찰과 고등학생의 자아 존중감에 관한 실태 조사)

  • 박진희;장남기
    • Hwankyungkyoyuk
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    • v.10 no.2
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    • pp.63-74
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    • 1997
  • 'Self-esteem' is defined as 'the lived status of one's individual competence and personal worthiness in dealing with the challenges of Life over Time' and high self-esteem is associated with self-confidence, effectively coping, well-being, and responsibility. The first purpose of this study was to identify the concept of self-esteem and to investigate the relationship with self-esteem and responsible environmental behaviors (REB). According to the results, it was found that 'self-esteem' is considered as one of the influential factors on REB and self-esteem enhancement is essential for the responsible choice and determination of environments. The second purpose was to analyse the level of self-esteem in High School students. The mean scores of merits and demerits for all the students involved (402) were 14.58 and 14.91 respectively. By applying the t-test, the two scores were not so different: thus the mean of self-esteem was found to be at a median level. The number of adove-average, average and below-average levels were 114, 163 and 125 respectively. From now on, the development of strategies to enhance self-esteem is urgently needed.

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Radial Electrical Impedance: A Potential Indicator for Noninvasive Cuffless Blood Pressure Measurement

  • Huynh, Toan Huu;Chung, Wan-Young
    • Journal of Sensor Science and Technology
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    • v.26 no.4
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    • pp.239-244
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    • 2017
  • Noninvasive, cuffless, and continuous blood pressure (BP) monitoring is essential to prevent and control hypertension. A well-known existing method for this measurement is pulse transit time (PTT), which has been investigated by many researchers as a promising approach. However, the fundamental principle of the PTT method is based on the time interval taken by a pulse wave to propagate between the proximal and distal arterial sites. Consequently, this method needs an independent system with two devices placed at two different sites, which is a problem. Even though some studies attempted to synchronize the system, it is bulky and inconvenient by contemporary standards. To find a more sensitive method to be used in a BP measurement device, this study used radial electrical bioimpedance (REB) as a potential indicator for BP determination. Only one impedance plethysmography channel at the wrist is performed for demonstrating a ubiquitous BP wearable device. The experiment was evaluated on eight healthy subjects with the ambulatory BP monitor on the upper arm as a reference. The results demonstrated the potential of the proposed method by the correlation of estimated systolic (SBP) and diastolic (DBP) BP against the reference at $0.84{\pm}0.05$ and $0.83{\pm}0.05$, respectively. REB also tracked the DBP well with a root-mean-squared-error of $7.5{\pm}1.35mmHg$.

The Effect of 'Self Control Management Strategy' on the Reinforcement of Internal Locus of Control (내적 조절점 강화를 위한 자기 조절 관리 전략의 효과)

  • Park, Jin-Hee;Chang, Nam-Kee
    • Journal of The Korean Association For Science Education
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    • v.17 no.4
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    • pp.347-358
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    • 1997
  • Locus of Control of Reinforcement (LOCR)' is considered as one of the most important factors on responsible environmental behaviors(REB). It is defined as 'an individual's perception of his or her ability to bring about change through his or her behavior'. This psychological construct is divided up into two directions: the external locus and internal locus. The internal locus is important as a predictor of REB. Recently, 'The Environmental Action Internal Control Index: EAICI' has been developed and it is a valid and reliable instrument to measure the relationship of two variables. The purposes of this study were to develop a strategy to reinforce the internal locus or to converse external locus to internal locus. and to verify the effects by EAICI. A new strategy, 'Self Control Management Strategy' was based on the characteristics of internal locus and the key idea of this was to self-control and to manage the courses by themselves. EAICI scores of the control group was 99.83. All of them were internal and the LOCR of females was more internal than that of the males. This strategy was applied when the instructions were carried out on seven environmental issues by the experimental group. According to the results. the seven points of the EAICI were increased significantly. Therefore this strategy is helpful in reinforcing the internal locus or to converse external locus to the internal locus.

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Severe Bilateral Microphthalmia in a Holstein Calf

  • Hur, Tai-Young;Kang, Seog-Jin;Jung, Young-Hun;Do, Yoon-Jung;Choi, Chang-Yong;Ryu, Il-Sun;Cho, Sung-Whan;Song, Kun-Ho;Suh, Guk-Hyun
    • Journal of Veterinary Clinics
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    • v.25 no.5
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    • pp.411-414
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    • 2008
  • This report described a severe bilateral microphthalmia in a young Holstein calf. The anomalous calf with wry tail showed normal vigor, appetite and normal body weight except for eye defect. The orbits were shallower and smaller than normal. The orbit bilaterally contained a white small mass suspected as eyeball. A spot-like remnant of eyeball (REB) was buried in the mixture of vestigial extraocular muscles and adipose tissue of the bilateral orbit. Histologically, the REB was composed of irregularly arranged elements of ocular wall such as sclera, retina and ciliary body. But any destructive changes in the central nervous systems were not detected. This case of eye defect was defined as severe bilateral microphthalmia. The cause of this ocular defect is unknown.