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Antioxidant Activity and Functional Component Analysis of Korean Mountain Ginseng's Different Sections (장뇌삼 부위별 기능성 성분분석과 항산화활성)

  • Kim, Jun-Han;Kim, Jong-Kuk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.10
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    • pp.1315-1321
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    • 2006
  • This study was conducted to investigate antioxidant activity and functional component analysis in different sections of Korean mountain ginseng (seed, leaf, stem and root). The major free amino acids were arginine, proline, $\gamma-amino-n-butyric$ acid, alanine and aspartic acid, and proline showed the highest content 22.98 mg/g in 80% MeOH extract of seed. Contents of $\gamma-amino-n-butyric$ acid and alanine were the highest in 80% MeOH extract of leaf, with 26.04 mg/g and 13.07 mg/g. Aspartic acid showed the highest content 23.42 mg/g in 80% MeOH extract of leaf. The major fatty acids were identified as palmitic acid, oleic acid and linoleic acid by GC. Content of total phenolic compounds were 737 mg% in 80% EtOH extract of seed, and 560 mg% in 80% MeOH extract of seed. The highest contents of ferulic acid and salicylic acid were $875{\mu}g/g\;and\;78{\mu}g/g$ in 80% MeOH extract of leaf, and $\rho-coumaric$ acid was $181{\mu}g/g$ in 80% MeOH extract of stem, respectively. The highest contents of ginsenoside-Rg1, Re, Rd and Rc were $14.5{\mu}g/g,\;48.78{\mu}g/g,\;27.57{\mu}g/g\;and\;4.87{\mu}g/g$ in 80% MeOH extract of leaf, respectively. Antioxidant activities by DPPH were 83.82% in water extract of leaf, 89.74% in 80% EtOH extract of leaf and 88.37% in 80% MeOH extract of leaf, and 92.81% in BHA (200 ppm). These results suggest that Korean mountain ginseng is very important as functional food material.

Process Optimization of Ginseng Berry Extract Fermentation by Lactobacillus sp. Strain KYH isolated from Fermented Kimchi and Product Analysis (발효 김치로부터 분리한 Lactobacillus sp. Strain KYH를 이용한 진생베리 추출물 최적 발효 공정 확립 및 생성물의 특성 분석)

  • Ha, Yoo-Jin;Yoo, Sun-Kyun;Kim, Mee Ree
    • Journal of the East Asian Society of Dietary Life
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    • v.26 no.1
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    • pp.88-98
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    • 2016
  • The pharmacological effects of ginseng berry have been known to improve psychological function, immune activities, cardiovascular conditions, and certain cancers. It is also known that fermentation improves the bioavailability of human beneficial natural materials. Accordingly, we investigated the optimal fermentation conditions of ginseng berry extract with strain isolated from conventional foods. We also analyzed the fermentation product and its antioxidant activity. The bacterium isolated from fermented kimchi was identified as Lactobacillus sp. strain KYH. To optimize the process, fermentation was performed in a 5 L fermenter containing 3 L of ginseng berry extract at 200 rpm for 72 hr. Under optimized conditions, batch and fed-batch fermentations were performed. After fermentation, organic acids, amino acids, sugars, ginsenosides, and antioxidant activity were evaluated. The optimum fermentation conditions were determined as pH 7.0 and a temperature of $30^{\circ}C$, respectively. After fermentation, the amounts and compositions of organic acids, amino acids, sugars, ginsenosides, and antioxidant activity were altered. In comparing the distribution of ginsenosides with that before fermentation, the ginsenoside Re was a major product. However, amounts of ginsenosides Rb1, Rc, and Rd were reduced, whereas amounts of ginsenosides Rh1 and Rh2 increased. Total phenol content increased to 43.8%, whereas flavonoid content decreased to 19.8%. The DPPH radical scavenging activity and total antioxidant activity increased to 27.2 and 19.4%, respectively.

The Effect of Exercise on Pulmonary Function (운동이 폐기능에 미치는 영향)

  • Park, Jae-Seuk;Kim, Youn-Seup;Choi, Eun-Kyoung;Jee, Young-Koo;Lee, Kye-Young;Kim, Keun-Youl;Chun, Yong
    • Tuberculosis and Respiratory Diseases
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    • v.45 no.2
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    • pp.351-359
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    • 1998
  • Background: The effects of exercise on pulmonary function are complex and have been the subject of many investigations. But, there has been disputes about the effect of exercise on spirometric parameters and there is no study about the effect of exercise on IOS(Impulse Oscillometry)parameters. IOS, a new method of pulmonary function test, is based on the relationship between the pressure and flow oscillation which is produced by applying sinusoidal pressure oscillation to the respiratory system via the mouth. Method: Fifty-nine young adults without respiratory symptoms were divided into three groups according to degree of exercise(hard exercise group: mean exercise time is over three hours per week at least for the last one month, light exercise group : between thirty minutes to three hours, nonexercise group : less than thirty minutes) and undertaken pulmonary function test(simple spirometry and IOS). Results: The effects of exercise on spirometric parameters; percentage of predictive value of forced vital capacity(FVC % pred) was higher in hard exercise group than nonexercise group(hard exercise group: $102.4{\pm}14.8$, nonexercise group: $93.7{\pm}9.9$, p=0.017), but there was no significant difference in percentage of predicted value of forced expiratory volume in one second(FEV 1 % pred) and percentage of predicted value of forced expiratory flow 50% (FEF 50% pred) between groups. The effects of exercise on IOS parameters: Reactance at 5Hz(X5) was significantly lower in hard exercise group than nonexercise group(hard exercise group: $-0.166{\pm}0.123hPa/1/s$, nonexercise group: $-0.093{\pm}0.036hPa/1/s$, p=0.006) but there was no significant difference in central resistance(Rc), peripheral resistance(Rp), resonance frequency(RF) and resistance at 5Hz, 20Hz between groups. Conclusion: Hard exercise increased FVC % pred on spirometric parameters and decreased reactance at 5Hz(X5) on IOS parameters.

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Saponins of Korean Ginseng Panax ginseng C.A. Meyer (Part III) -Saponins of ginseng by the cultivating locations, sampling seasons, plant parts, growing stages and the processings- (한국인삼(韓國人蔘)의 Saponin에 관(關)한 연구(硏究)제3보(第三報) -산지별(産地別), 부위별(部位別), 재배기간별(栽培期間別) 인삼(人蔘) 및 가공중(加工中) Saponin함량(含量)에 관(關)하여-)

  • Cho, Sung-Hwan
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.188-204
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    • 1977
  • The studies on the saponins of Korean ginseng, Panax ginseng C.A. Meyer, were performed according to the cultivating locations, sampling seasons, plant parts, and growing stages. The changes in saponin content in the course of manufacturing Red ginseng and Ginseng extract were observed. In this paper, a new method for the determination of the total and the individual saponin glucosides was proposed and applied to the samples under study. The method employing Digital Densitorol DMU-33C (Toyo electric Co., Japan) followed the separation of the saponins by means of a preparative thin layer chromatography. The saponin contents and their fractional distribution were summarized as follows: 1. The average concentrations(% plant dry weight) of semi-purified saponins in the roots of Korean ginseng planted in the various locations were 5.0%(Keumsan), 6.0% (Kimpo), and 5.4% (Pocheon), respectively. 2. There were 3.3% saponins in White ginseng(Rhizome) and 12.7% saponins in Ginseng tail (Fibrous root). 3. Regarding the year of growth, the contents of saponins were 90.3mg (2-year-old ginseng), 254.4mg (3-year-old ginseng), 404.2mg (4-year-old ginseng). 999.6mg (5-year-old ginseng), and 1377.1mg (6-year-old ginseng) respectively, and the saponin factions containing panaxatriol as an aglycone increased. 4. Thin layer chromatography revealed that Red ginseng yielded many saponins which Shibata et al. designated as $ginsenoside-Rb_1$ (22.1%), $-Rb_2(15.4%)$, -Rc(12.6%), -Re (15.7%), and $-Rg_1$, (9.3%). 5. 29.9% of crude saponins were isolated from ethanolic extract of Panax ginseng fibrous root and their extraction yield was 94.2% of fibrous root saponin.

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The Results of Arthroscopic Double-Row Rotator Cuff Repairs with Combined Knot-tying and Knotless Suture Anchors (매듭 결속과 비매듭 봉합나사를 이용한 관절경적 이열 회전근개 봉합술의 결과)

  • Ku, Jung-Hoei;Lee, Choon-Key;Cho, Hyung-Lae;Choi, Seung-Hyun
    • Journal of the Korean Arthroscopy Society
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    • v.12 no.3
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    • pp.172-179
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    • 2008
  • Purpose: To evaluate the functional and structural results of arthroscopic double-row repair using combined knot-tying and knotless suture anchors in rotator cuff tears. Materials and Methods: From March 2006 to June 2007, twenty-one patients (15 males, 6 females; mean age 55.6 years; range 48 to 67) were included who underwent arthroscopic double-row repair for full-thickness tears of the rotator cuff following conservative treatment for a mean of 6.5 months (range 3 to 11). The tear size was carefully inspected arthroscopically and we found 2 small, 13 medium and 6 large-sized rotator cuff tears, with a mean tear size of 2.5cm(range 1.8 to 3.2). The repair constructs were consisted of horizontal mattress sutures using conventional knot-tying suture anchors medially and simple suture at the same level of medial row stitch with Bioknotless RC anchors (DePuy Mitek, Norwood, MA) as lateral row. Clinical and functional evaluations were made according to the range of motion, the ASES, UCLA scale and the isokinetic strength testing. Postoperative cuff integrity was determined through magnetic resonance imaging. The mean follow-up was 15 months (range 13 to 24). Results: The average clinical outcome scores and strength were all improved significantly at the time of the final follow-up (p < 0.01). Nineteen patients (90%) were satisfied with the result of the treatment. In 17 of 21 patients (81%) were judged to reveal healed tendon on magnetic resonance imaging at a mean of 7 months postoperatively. There were no significant functional differences according to the preoperative tear size (p<0.01), but large-sized tear shows less favorable structural results in 3 out of 6 cases(50%). Conclusion: Our results document the usefulness and variability of arthroscopic double-row rotator cuff repairs comparable to the results of the other types of double-row repairs.

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Antioxidant Activity of Methanol Extracts from Cudrania tricuspidata Bureau according to Harvesting Parts and Time (이용부위 및 채취시기에 따른 꾸지뽕나무 메탄올 추출물의 항산화성)

  • Choi, So-Ra;You, Dong-Hyun;Kim, Jong-Yeob;Park, Chun-Bong;Kim, Dae-Hyang;Ryu, Jeong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.2
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    • pp.115-120
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    • 2009
  • To obtain the information on antioxidant activity by harvesting parts and time in Cudrania tricuspidata Bureau, total phenolic contents, flavonoid contents, superoxide dismutase (SOD) like activity and 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity of methanol extracts from Cudrania tricuspidata were investigated. Total phenolic contents was the highest in leaf [$30.2{\sim}38.8\;mg/g$ dry weight (DW)] and followed by root bark, stem bark, fruit, root except bark and stem except bark. Among harvesting time, total phenolic contents of leaf was the highest as $38.1{\sim}38.8\;mg/g$ DW in May and June, and then decreased to October. Root bark harvested in April and May contained $26.6{\sim}27.8\;mg/g$ DW total phenolic compounds and total phenolic content fell to a very low values at 1.8 mg/g DW in February. Flavonoid contents was very high in leaf and root bark. Especially, root bark harvested in April had the highest of flavonoid contents as 23.2 mg/g DW. As a result of simple linear regression analysis of total phenolic contents on flavonoid contents according to harvesting parts, it showed a high correlation (p < 0.001) with $0.57{\sim}0.97\;R^2$ (coefficient of determination). As $RC_{50}$ value, that was, the concentration of sample required for 50% reduction of DPPH absorbance, was $13.7{\sim}20.5\;{\mu}g/mL$ in leaf so DPPH radical scavenging activity was very high. But SOD like activity of $1,000\;{\mu}g$ extract/mL MeOH was a low value of $0.3{\sim}9.2%$ in all samples.

Fragility Analysis Method Based on Seismic Performance of Bridge Structure considering Earthquake Frequencies (지진 진동수에 따른 교량의 내진성능기반 취약도 해석 방법)

  • Lee, Dae-Hyoung;Chung, Young-Soo;Yang, Dong-Wook
    • Journal of the Korea Concrete Institute
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    • v.21 no.2
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    • pp.187-197
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    • 2009
  • This paper presents a systematic approach for estimating fragility curves and damage probability matrices for different frequencies. Fragility curves and damage probability indicate the probabilities that a structure will sustain different degrees of damage at different ground motion levels. The seismic damages are to achieved by probabilistic evaluation because of uncertainty of earthquakes. In contrast to previous approaches, this paper presents a method that is based on nonlinear dynamic analysis of the structure using empirical data. This paper presents the probability of damage as a function of peak ground acceleration and estimates the probability of five damage levels for prestressed concrete (PSC) bridge pier subjected to given ground acceleration. At each level, 100 artificial earthquake motions were generated in terms of soil conditions, and nonlinear time domain analyses was performed for the damage states of PSC bridge pier structures. These damage states are described by displacement ductility resulting from seismic performance based on existing research results. Using the damage states and ground motion parameters, five fragility curves for PSC bridge pier with five types of dominant frequencies were constructed assuming a log-normal distribution. The effect of dominant frequences was found to be significant on fragility curves.

A TCP-like flow control algorithm for RTP/RTCP (TCP 와 RTP/RTCP 유사한 흐름제어 알고리즘)

  • 나승구;윤성덕;안종석
    • Proceedings of the Korean Information Science Society Conference
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    • 1998.10a
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    • pp.480-482
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    • 1998
  • 최근, 멀티캐스트 기법을 사용하는 멀티미디어 응용 프로그램들이 인터넷에 등장하고 있다. 이들 응용 프로그램들의 성공 여부는 수신자들에게 전송되는 음성/영상의 품질에 의해 좌우된다. 인터넷은 응용프로그램의 QoS(Quality of Service) 에 대한 요구를 보장할 수 없기 때문에 멀티케스트 트래픽(multicast traffic)을 위하여 인터넷의 성능을 최대한 효율적으로 이용할 수 있도록 흐름제어에 대한 많은 연구가 진행되고 있다. 그 중 IVS(INRIA Video conferencing System)에서 제안한 멀티캐스트 트래픽 흐름제어 알고리즘은 수신자가 주기적으로 전달하는 RTCP 의 패킷손실 정보에 의해 송신자가 전송율을 조절하는 것이다. 그러나 이 알고리즘은 네트워크 상태가 무부하(unload)임에도 불구하고 느린 피드백으로 인하여 가용 네트워크 대역폭을 빠르게 파악하지 못하기 때문에, TCP트래픽과 경쟁 상태에서 네트워크 대역폭을 불공정(unfairness)하게 사용하게 되고 네트워크 상태에 알맞는 전송율을 결정하지 못한다. 본 논문에서는 더욱 공정하게 대역폭을 공유할 수 있고 전체 링크 이용율을 높이는 두 가지 기법을 제안한다. 첫째, 측정된 네트워크 혼잡상태에 따라 RTCP 피드백의 전송 빈도를 동적으로 조절하는 것이다. 둘째, TCP와 같이 전송율을 증가/감소시킴으로써 공정하게 네트워크를 공유하도록 하는 것이다. 본 논문에서는 이 두 가지 기법들이 TCP 트래픽에 영향을 주지 않고 또한 RTCP피드백의 양을 증가시키지 않으면서도 공정하게 네트워크 대역폭을 공유함으로써 링크의 이용율을 높일 수 있다는 것을 시뮬레이션을 통하여 보여준다.안 모니터링 기 능 등으로 조사되었다.도 멜-켑스트럼을 사용한 경우 67.5%, K-L계수를 사용한 경우 75.3%로 7.8%의 향상된 인식률을 보였으며 K-L계수와 회귀계수를 결합한 경우에서도 비교적 높은 인식률을 보여 숫자음에 대해서도 K-L계수의 유효성을 확인할 수 있었다..rc$ 구입할 때 중점적으로 살펴보는 사항은 신선도와 순수재래종 여부, 위생상태였다. 한편 소비자가 언제나 구입할 수 없다는 의견이 85.2%나 되어 원활한 공급과 시장조성이 아직 정착되지 않고 있었다. $\bigcirc$ 현재 유통되고 있는 재래종닭은 소비자 대부분이 잡종으로 인식하고 있었으며, 재래종과 일반육계와의 구별은 깃털색, 피부색, 정강이색등 외관상으로 구별하고 있었다. 체중에 대한 반응은 너무 작다는 의견이었고, 식품으로의 인식도는 비교적 고급식품으로 인식하고 있다. $\bigcirc$ 재래종닭고기의 브랜드화에 대한 견해는 젊고 소득이 높은 계층에서 브랜드화의 필요성을 강조하고 있다. $\bigcirc$ 재래종달걀의 소비형태는 대부분의 소비자가 좋아하였으나 아직 먹어보지 못한 응답자가 많았다. 재래종달걀의 맛에 대해서는 고소하고 독특하여 차별성을 느끼고 있었다. $\bigcirc$ 재래종달걀의 구입장소는 계란판매점(축협.농협), 슈퍼, 백화점, 재래닭 사육 농장등 다양하였으며 포장단위는 10개를 가장 선호하였고, 포장재료는 종이, 플라스틱, 짚의 순으로 좋아하였다. $\bigcirc$ 달걀의 가격은 200원정도를 적정하다고 하였으며, 크기는 (평균 52g)는 가장 적당하다고

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Experimental Study on Hysteretic Behavior of 100 MPa Ultra High-Strength Concrete Tied Columns (100 MPa 초고강도 콘크리트 띠철근 기둥의 이력거동에 관한 실험적 연구)

  • Kim, Jong-Keun;Shin, Sung-Woo
    • Journal of the Korea Concrete Institute
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    • v.18 no.2 s.92
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    • pp.161-168
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    • 2006
  • An experimental investigation was conducted to examine the hysteretic behaviors of ultra-high strength concrete tied columns. The purpose of this study is to investigate the safety of ultra-high strength concrete columns with 100 MPa compressive strength for the requirement of ACI provisions. Eight 1/3 scaled columns were fabricated to simulate an 1/2 story of actual structural members with the cross section $300{\times}300mm$ and the aspect ratio 4. The main variables are axial load ratio, configurations and volumetric ratios of transverse reinforcement. The results show that the deformability of columns are affected by the configurations and volumetric ratios of transverse reinforcement. Especially, it has been found that the behavior of columns are affected by axial load ratio rather than the amounts and the configurations of transverse reinforcement. Consequently, to secure the ductile behavior of 100 MPa ultra-high strength concrete columns, ACI provisions for the requirement of transverse steel may considered axial load level and the details of transverse reinforcement.

Investigation on Response Modification Factor of RC Structural Walls in Apartment Buildings (아파트 건물의 구조 벽체에 대한 반응수정계수)

  • 한상환;오영훈;이리형
    • Journal of the Korea Concrete Institute
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    • v.13 no.6
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    • pp.544-552
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    • 2001
  • Korea is classified into low and moderate seismic zone from the view-point of seismic hazard level. Korean seismic provisions has been developed based on UBC and ATC 3-06. Thus, in calculation of design base shear according to Korean provisions response modification factor (R) is included in the formula of design base shear. The major role of this factor is to reduce the elastic design base shear whereby structures can behave in inelastic range during design level earthquake ground motions(mean return period of 475 yrs.). R factor is assigned according to material and structural systems. In this study, R factor for bearing wall system is considered. Most of the walls of apartment buildings in Korea resist gravity and seismic loads simultaneously so that this wall system can be classified into bearing wall system. Structural details of these walls are different from those used in Japan and U.S.. They are all rectangular in sectional shape rather than barbell in shape, and also have special lateral reinforcement details at the boundaries of a wall. In Korean seismic design provisions(1988), two different values(3.0 and 3.5) of R factor are assigned to the bearing wall systems according to the wall details. However, in updated seismic provisions(2000), only one value is assigned to R factor(3.0) irrespective of wall details. In this study, the design base shear values in Korean seismic design provisions(1988, 2000), ATC 3-06, UBC are compared. Also experimental study was carried out to evaluate the seismic performance of structural walls. For this purpose, five test specimens were made which have special details used in apartment bearing wall systems in Korea. Based on the results of this study, response modification factor for bearing wall system is discussed.