• ALGAE
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• v.35 no.4
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• pp.337-347
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• 2020

Haraldiophyllum hawaiiense sp. nov. is described as a new mesophotic alga and a new genus record for the Hawaiian Islands. Six specimens were collected at a depth range of 81-93 m from Papahānaumokuākea Marine National Monument, and their morphology investigated, as well as molecular phylogenetic analyses of the plastidial ribulose-1,5-bisphosphate carboxylase-oxygenase large-subunit (rbcL) gene and a concatenated alignment of rbcL and nuclear large-subunit rRNA gene (LSU) sequences. Phylogenetic analyses supported H. hawaiiense sp. nov. as a distinct lineage within the genus Haraldiophyllum, and sister to a large clade containing the type species, H. bonnemaisonii, as well as H. crispatum and an undescribed European specimen. The six Hawaiian specimens were shown to be identical, but unique among other species of the genus as well as the recently segregated genus Neoharaldiophyllum, which comprises half of the species previously included in Haraldiophyllum. The vegetative morphology of H. hawaiiense sp. nov. resembles Neoharaldiophyllum udoense (formerly H. udoensis); however, no female or post-fertilization structures were found in the Hawaiian specimens to allow a more comprehensive comparison. The molecular phylogenies demonstrate that Haraldiophyllum is paraphyletic, suggesting either that the Myriogrammeae tribe includes undescribed genera, including Haraldiophyllum sensu stricto, or that Neoharaldiophyllum species should be transferred into the genus Haraldiophyllum. However, based on vegetative morphology and molecular analyses, and pending resolution of this taxonomic issue, the Hawaiian specimens are placed within the genus Haraldiophyllum. This new record for the Hawaiian Islands highlights the novel biodiversity from mesophotic depths, reaffirming the need for further investigation into the biodiversity of Mesophotic Coral Ecosystems.

• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.135-142
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• 2009

Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.101-105
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• 2020

Ralfsia longicellularis is known as an endemic species in Far East Asia. In this study, we report R. longicellularis as a new record from Korea based on morphological and molecular analyses. Molecular analyses based on plastid-encoded rbcL gene sequences and morpho-anatomical studies were undertaken on Ralfsia species, a poorly studied genus from Korea. Ralfsia longicellularis is mainly characterized by a dark brown thallus; 770-1200 ㎛ thick, curved cells in the creeping and ascending parts of the filaments; basal layer and erect filament cells with a width-to-length ratio of 1 : 1.5 to 10; narrowshaped sporangia on-stalk cells at the base of the paraphyses; and mostly uniseriate plurilocular reproductive organs capped with 1-2 sterile cells. The R. longicellularis samples from Korea in this study were similar to ones collected from the type locality (Peter the Great Bay, Russia) in morphology. The rbcL analyses also revealed that our Korean R. longicellularis samples were placed in the same clade with Russian materials, within a Ralfsia clade but distinct from the congeners.

• ALGAE
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• v.24 no.2
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• pp.79-83
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• 2009

Recently deschbed new species, Cladosiphon umezakii Ajisaka (Ectocarpales,Phaeophyceae) is reported in Korea based on morphology and plastid rbcL sequences. Cladosiphon umezakii occurred on low intertidal to subtidal zone along the south and southeast coasts in Korea. Thalli are gold to dark brown, ercct, cylindrical, irregularly branched,and very mucoid. Unitocular zoidangium is developed at the base uf assimilatory filaments. Assimilatory filaments are very long. Eight specimens of the pecies collected from Korea clustered with those of C. umezakii in Japan in rbcL tree. The pecies showed a sister relationship with C. okamuranus. The occurrence of this warm water species is thought an example of northward migration of marine algae caused by global warming. Because C. umezakii is of the economica! importance of the genus as foods and medidnes, the study on mass culture of the species is necessary in korea.

• ALGAE
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• v.33 no.2
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• pp.143-156
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• 2018

Dolichospermum is a filamentous and heterocytous cyanobacterium that is one of the commonly occurring phytoplanktons in the Han River of Korea. Morphological observations led to the identification of D. planctonicum-like filaments in seasonal water samples. In the present study, we successfully isolated these filaments using culture methods, and examined its morphology using light and scanning electron microscopy. The morphology of the D. planctonicumlike species differed from that of typical D. planctonicum; it had thin cylindrical-shaped akinetes, which were narrower towards the ends than at the center. This morphology is firstly described in the genus Dolichospermum. In addition, the akinetes in the filament developed solitarily and were distant from the heterocytes. Phylogenetic analysis of the 16S rRNA sequences showed that our Dolichospermum clustered with D. planctonicum and D. circinale, which have coiled trichome. However, phylogenetic analysis of the gene encoding rivulose-1,5-bisphosphate carboxylase (rbcLX) clearly separated our species from other Dolichospermum, forming a unique clade. Additionally, structures of D. planctonicum and D. hangangense strains were different type in Box-B and V3 region. These results demonstrated that the new Dolichospermum species was unique in morphology and molecular traits. Therefore, we propose this to be a new species belonging to genus Dolichospermum with the name Dolichospermum hangangense sp. nov.

• ALGAE
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• v.19 no.3
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• pp.161-174
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• 2004

Phonological, morphological and molecular characteristics of Spatoglossum pacificum Yendo are examined. S. pacificum has an annual life cycle composed of saprophytes with apparent absence of male and female gametophytes in Korea. The seasonal growth of this species explains that the annual growth is closely related to the monthly variation of water temperature. S. pacificum has protruding reproductive structures above the outmost cortical layer. Although this observation is restricted to several species, reproductive structures on the thallus can make S. pacificurn distinguishable from S. crassum and S. lacturn. The morphogenesis of a midrib at the base of S. pacificum in this study is the same as those of Dictyopteris but different from those of S. crassum and S. lacturn, suggesting that S. pacificum is closely related to Dictyopteris. In the comparison of plastid gene sequences among species of Spatoglossum and Dictyopteris, S. pacificum is more similar to D. divaricata and D. undulate than those of S. crassum in rbcL, rbcS, psbA and psaA. This result is congruent with the anatomical characteristic of a midrib at the base of the thallus and the protrusion of reproductive organs on the thallus. The phylogenetic relationship based on these plastid genes also shows that S. pacifism is included in Dictyotpteris Glade and separated from S. crassum. We propose the new combination of Dictyopteris pacifica (Yendo) I.K. Hwang, H.S. Kim et W.J. Lee, comb. nov. based on the differences of anatomical characteristics of the midrib, the existence of reproductive organs on thallus and the molecular analyses.

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• Fisheries and Aquatic Sciences
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• v.20 no.10
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• pp.25.1-25.5
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• 2017

An economic marine alga, which is considered to be an important source of carrageenan, was collected from Jindo of the southern coast of Korea. This species shares the vegetative and female reproductive features of Ahnfeltiopsis and is characterized mostly by its small size (up to 8 cm), terete to subterete thalli at the lower portion, cartilaginous in texture, dichotomous branches, rarely produced proliferations, and an absence of hypha-like filaments in the medulla. It is distinguished from other Korean species within the genus by the thallus feature. In a phylogenetic tree based on the molecular data, this alga nests in the same clade with A. concinna from Japan but forms a sister clade to A. concinna from Mexico and Hawaii (type locality). However, the genetic distance among those sequences was calculated as 0.1-1.3% for rbcL and 1.1% for COI sequences, considered to be intraspecific variation within the genus. Based on the morphology and molecular analysis, this alga is identified as A. concinna originally described from Hawaii. This is the first record of the species in the Korean marine algal flora.

• ALGAE
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• v.30 no.3
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• pp.197-205
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• 2015

The genus of leafy green algae, Prasiola Meneghini, includes marine, terrestrial, and freshwater species. A total of 11 species and one variety have been identified in China, Korea, and Japan. In Korea, Prasiola formosana var. coreana has been reported in Muncheon, North Korea, while a different type of Prasiola species has been reported in South Korea. The South Korean species has been found growing along a small stream originating from Chodanggul Cave, a limestone cave in Samcheok, Gangwon Province. Here, we revised the morphological characteristics of the South Korean Prasiola species and analyzed plastid rbcL, psaB, and tufA genes to clarify its identity. Although the external and anatomical morphologies varied among individuals, our results were very similar to previous reports. Plastid three genes sequences of the South Korean specimens were identical to those of P. japonica collected from Japan as well as to published sequences of P. yunnanica from China. A short rbcL-3P sequence (196 bp) from P. formosana var. coreana, which was identified in the type specimen, was also identical to a sequence from P. japonica. These Prasiola species and variety from Korea, Japan, and China are all distributed in areas characterized by limestone bedrock. Based on morphological, phylogenetic, and distributional features, the South Korean Prasiola species is regarded herein as P. japonica. Here, we also propose to synonymize P. formosana var. coreana and P. yunnanica with P. japonica.

• ALGAE
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• v.29 no.1
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• pp.1-13
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• 2014

The genus Erythroglossum is characterized by Phycodrys-type apical organization, Polyneura-type procarp, and the presence of a midrib. We collected an unidentified Delesseriaceaen species from deep water off the southern coast of the Korean Peninsula. This alga resembles Polyneura japonica in terms of having broadly flattened thalli with a cylindrical stipe, the presence of a midrib and alternative lateral veins. To confirm the taxonomic status of this entity, we compared the morphological features and rbcL sequences among other species of Erythroglossum and P. japonica. As a result, we assigned the new species, Erythroglossum hyacinthinum, to the genus Erythroglossum because of the presence of a midrib. This species is characterized by an elliptical to obovate blade with a short cylindrical stipe, a conspicuous midrib and alternate veins, margins with numerous microscopic dentations, di-trichotomously branching, and bulish-violet iridescence. The phylogeny of rbcL sequences indicates that E. hyacinthinum is definitely a separate entity, but the genera in the tribe Phycodryeae have inconsistent phylogenetic relationships. This is the first study comparing the molecular phylogeny within the genus Erythroglossum.