• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1322-1330
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• 2016

This experiment was conducted to determine standardized ileal amino acid digestibility (SIAAD) of commonly used feed ingredients in poultry diets in Pakistan. These feed ingredients included corn, rice broken (RB), rice polishings (RP), wheat bran (WB), sunflower meal (SFM), cottonseed meal (CSM), guar meal (GM), soybean meal (SBM) from India and Argentine and fish meal (FM). The SIAAD of each ingredient was determined in triplicate using 21-days-old broilers. Day-old male broiler chicks (Hubbard${\times}$ Hubbard) were reared on corn-SBM based diet from 1 to 13 days and thereafter birds were fed experimental diets from day 14 to 21. Each diet was fed to 36 birds kept in six replicate cages, each cage had six birds. In cereals, the SIAAD of corn's amino acid (AA) (90.1%) was similar (p>0.05) to RB (89.0%). Isoleucine (97.8%) and lysine (96.9%) were highly digestible AA in corn and RB, respectively. Among cereal-by products, WB's SIAAD (76.9%) was same (p>0.05) as RP (71.9%). Arginine from WB (82.5%) and RP (83.2%) was highly digestible. However, threonine in WB (72.7%) and leucine in RP (69.6%) were the lowest digestible AAs. In plant protein meals, AAs from Argentine-SBM (85.1%) and Indian-SBM (83.4%) had higher (p<0.5) SIAAD than other protein meals. However, SIAAD of SFM (77.1%) and CSM (71.7%) was intermediate while GM (60.3%) exhibited the lowest (p<0.05) SIAAD among all ingredients. Arginine from GM (76.9%), CSM (85.8%), SBM-India (89.5%) and SBM-Argentine (91.5%) was highly digestible from indispensable AAs. In SFM, methionine (91.4%) SIAAD was the greatest. The average SIAAD of FM was 77.6%. Alanine from FM had the highest (84.0%) but cysteine (62.8%) had the lowest SIAAD. In conclusion, cereals i.e. corn and RB had higher (p<0.05) SIAAD of the cereals by-products. The SIAAD of RP and WB was same (p>0.05). The SBM from plant protein meals had higher (p<0.05) SIAAD than other studied feed ingredients. However, the GM had the lowest (p<0.05) SIAAD among protein meals.

• Korean Journal of Poultry Science
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• v.27 no.1
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• pp.51-61
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• 2000

This study was conducted to estimate the general production performance, heritabilities, and genetic phenotypic correlations on several economic traits by multiple trait animal model in Korean native chicken. The data were collected from the records of 5,192 pullets produced from 2,029 dams and 250 sires in Daejon Branch, National Livestock Research Institute from April 10. 1994 to February 10. 1997. Body weights of Red Brown (RB), Yellow Brown (YB), Grey Brown (GB) and Black (B) strains were 1,649.9, 1,439.6, 1,455.6 and 1,575.3g at age of 150 days and 1,923.5, 1,689.6, 1,812.4 and 1,924.0g at age of 270 days, respectively. The ranges of coefficient of variation for body weight were 11.52 to 14.02 at age of 150 days and 12.82 to 14.59% at age of 270 days, respectively. The first eggs of RB, YB, GB and B strains were produced at 147.4, 146.5, 151.1 and 152.1 days, respectively and the coefficients of variation were 7.80 to 8.41%. Egg weight at 270 days were 49.7, 47.6, 48.8 and 48.7 g for RB, YB, GB nd B strains, and coefficients of variation were 6.99 to 7.94% . The number of eggs at age of 270days were 75.8, 78.0, 76.7 and 68.8 for RB, YB, GB and B strains, respectively, and coefficient of variations were 23.87 to 29.89%. On heritability estimates in RB, YB, GB and strains body weight were 0.40, 0.10, 0.09 and 0.57 for RB, YB, GB and B strains at age of 150 days, and 0.23, 0.09, 0.38 and 0.24 at age of 270 days were 0.39, 0.47, 0.60 and 0.62, and egg weights at 270 days and age at 1st egg were 0.36, 0.12, 0.38 and 0.26, number of egg production at 270 days were 0.44, 0.36, 0.58 and 0.49, respectively. The genetic correlation coefficients of body weight with the age of first egg, egg weight and number of egg production at 270days were -0.07∼0.67, -0.24∼0.70 and 0.12∼0.41, respectively ; age of first egg with number of egg production at 270days and egg weight were -0.75∼0.91 and 0.34∼0.97 ; and egg weight with number of egg production at 270days were 0.18∼0.68. The phenotypic correlation coefficients of body weight with at age of first egg, egg weight and number of egg production at 270days were -0.01∼0.74, -0.04∼0.72 and 0.25∼0.57 ; age of first egg with number of egg production at 270days and egg weight were 0.26∼0.52 and 0.52∼0.92, respectively ; and egg weight with number of egg production at 270days were 0.34∼0.91.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.1
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• pp.72-76
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• 1980

The present studies were carried out to develope a simple quantitative analysis for saponins in ginseng by hemolysis. Erythrocytes from pig, rabbit, human and cattle were useful for this purpose. Erythrocytes could be stored at $4^{\circ}C$for 9 days without altering the sensitivity to saponins. Ginsenoside-Rb_1 did hemolysis, but Ginsenoside-Re protected erythrocytes from hemolysis. The following formula is proposed for calculation of saponins from ginseng extract: X=120$\mu$g $\times$$V_2/V_1$

• Proceedings of the Korean Vacuum Society Conference
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• 1999.07a
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• pp.76-76
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• 1999

한국자원연구소 제작된 TOF spectrometer는 입자의 비행시간과 에너지를 동시에 측정 기록하여 질량분별된 에너지 스펙트럼을 얻고자 하는 목적으로 제작되었다. 그러나 질량분별이 필요치 않은 후방 산란실험에서는 비행시간의 측정만으로 훌륭한 에너지 계측기가 된다. 중이온 산란분석법(heavy ion RBS)은 He을 사용하는 산란분석법에 비하여 원리적으로 분해능이 우수하나 사용하는 반도체 검출기의 에너지 분해능의 불량이 이러한 장점을 상쇄하는 실정이다. 그러나 TOF spectrometer를 중이온 산란실험의 에너지 계측기로 사용하면 H, He에 대한 반도체 검출기 정도의 낮은 에너지분해능 수준을 유지할 수 있으므로 중이온 산란실험의 원리적인 장점을 살릴 수 있다. 한국자원연구소에서는 He RBS의 취약점으로 지적되어온 medium elements에 대한 질량분해능과 깊이 분해능을 향상시키고자 140$^{\circ}$ 산란각에 TOF spectrometer가 위치하는 Heavy Ion Rutherford Backscattering Spectrometry (HIRBS) 시스템을 설계하였다. 설계된 TOF spectrometer를 채용하였을 때 기대되는 질량분해능 향상효과와 분석의 최적조건에 관하여 논한다.

• Journal of the Korean Ceramic Society
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• v.22 no.3
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• pp.72-76
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• 1985

Single crystals of the compound MeI $(LiMoO_4)$ and $Me^I(LiWO_4)$ ($Me^I=K$, Rb, Cs) were synthesized by slow evaporation from aqueous solution and bycooling from melt. The compounds of potassium or rubidium are hygroscopic and they form easily hydrated crystals $Me^I LiMoO_4$.$H_2O$ or $Me^ILiMoO_4$.$H_2O$ or $Me^ILiWO_4$.$H_2O$ from aqueous solution. The structures of these hydrated crystals are each other isotypic and they are built up of distorted layers of $(LiMoO_5)$ or $(LiWO_5)$. There exist two types of tetrahedral framework structures in this group of anhydrous molybdates and tung-states ; tridymite-type and cristobalite-type. $KLiMoO_4$ and $KLiWO_4$ have two types of polymorphic structures where as only the cristobalite-type is found in the Rb-and Cs-compounds. The system $KLiSO_4-KLiMoO_4$ was studied. Two components are almost immiscible but there eixst a narrow area of solid solution on the side of sulfate in the system.

• The Korean Journal of Physiology
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• v.30 no.2
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• pp.257-267
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• 1996

Diabetes mellitus is associated with a wide range of pathophysiologic changes in the kidney. This study was designed to examine the mechanisms by which glucose modulates the expression of polarized membrane transport functions in primary cultured rabbit renal proximal tubule cells. Results are as follows: The rate of 30 minute $Rb^{+}$ uptake was significantly higher($137.76{\pm}5.40%$) in primary renal tubular cell cultures treated with 20 mM glucose than that of 5 mM glucose. Not the level of mRNA for the ${\alpha}$ subunit of Na, K-ATPase but that of ${\beta}$ subunit was elevated in primary cultures treated with high glucose. The initial rate of methyl-${\alpha}$-D-glucopyranoside(${\alpha}$-MG) uptake was significantly lower($71.91{\pm}3.02%$) in monolayers treated with 20 mM glucose than that of 5 mM glucose. There was a tendency of an increase in phlorizin binding site in cells treated with 5 mM glucose. However, 3-O-methyl-D-glucose(3-O-MG) uptake was not affected by glucose concentration in culture media. TPA inhibited $Rb^{+}$ uptake by $63.61{\pm}1.94\;and\;45.80{\pm}1.36%$ and ${\alpha}$-MG uptake by $48.54{\pm}3.69\;and\;41.87{\pm}6.70%$ in the cells treated with 5 and 20 mM glucose, respectively. Also TPA inhibited mRNA expression of Na/glucose cotransporter in cells grown in 5mM glucose medium. cAMP significantly stimulated ${\alpha}$-MG uptake by $114.65{\pm}5.70%$ in cells treated with 5mM glucose, while it did not affect ${\alpha}$-MG uptake in cell treated with 20 mM glucose. However, cAMP inhibited $Rb^{+}$ uptake by $76.69{\pm}4.16\;and\;66.87{\pm}2.41%$ in cells treated with 5 and 20 mM glucose, respectively. In conclusion, the activity of the renal proximal tubular Na,K-ATPase is elevated in high glucose concentration. In contrast, the activity of the Na/glucose cotransport system is inhibited. High glucose may in part affect the activity of the Na,K-ATPase and the Na/glucose cotransport system by controlling the protein kinase C and/or A signal transduction pathway in primary cultured renal proximal tubule cells.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.1
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• pp.75-81
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• 2011

The effect of lactic acid bacteria.fermented ginseng berry extract (FGBE) on physiological activities was evaluated. The contents of ginsenosides Re, Rc, and Rb1 in ginseng berry extract (GBE) were increased after fermentation by lactic acid bacteria when analyzed by high performance liquid chromatography. Antioxidant activity of GBE and FGBE was also analyzed by DPPH radical scavenging activity assay and SOD.like activity assay. FGBE showed a 86.34 % inhibition of DPPH radical and a 76.82 % inhibition by SOD.like activity at a concentration of 1.00 %. GBE showed a 49.78 % inhibition of DPPH radical and a 40.80 % inhibition by SOD.like activity at the same concentration. Furthermore, procollagen type I (COL1A1) gene expression increased by 823.13 % and matrix metal-loproteinase(MMP)-1 and tumor necrosis factor (TNF)-${\alpha}$ gene expression decreased by 87.88 % and 99.92 %, respectively, in human fibroblast cultured with FGBE at a concentration of 0.50 %. These results suggest that FGBE could be used as an active ingredient for functional cosmetics.

• Journal of Ginseng Research
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• v.26 no.4
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• pp.196-201
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• 2002

This study was carried out to compare the contents of saponins and minerals component in Korean Red Ginseng (Heaven, Earth, Good grade), North Korean Red Ginseng (Heaven, Earth, Good grade), Japanese Red Ginseng (Oonju, Sinju 1, 2, 3 grade, respectively) and Chinese Red Ginseng (Seokju, Gilim, 1, 2, 3 grade, respectively). Crude saponin contents were different on according to the grade and cultivation area, and was 3.05-3.76% in Korean Red Ginseng, 2.09-3.21% in North Korean Red Ginseng, 2.82-3.71% in Chinese Seokju Red Ginseng, 2.72-3.62% in Chinese Gilim Red Ginseng, 2.11-2.44% in Japanese Oonju Red Ginseng, 2.18-2.87% in Japanese Sinju Red Ginseng, and the amount of ginsenoside-Rb1, -Re, -Rg$\_$1/ in Korean Red Ginseng were higher than those of North Korean, Chinese and Japanese Red Gingsen. The contents of mineral components were similar, but La, Na and Sn component in Korean Red Ginseng showed the higher amount than those of other Red Ginsengs.

• Journal of fish pathology
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• v.16 no.3
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• pp.161-164
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• 2003

An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi and Jechun area in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89)

• Korean Journal of Medicinal Crop Science
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• v.17 no.4
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• pp.251-256
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• 2009

The parts of leaves, flowers and stems in ginseng were obtained for analyzing the component of saponin on 15th April, 25th April, 5th May, 25th May, which were considered as ginseng foliation stage. The total saponin content of the leaves were 97.29, 66.42, 67.61, 36.24 mg/g, respectively, in which the content of Re, $Rb_1$ and Rd were more than 2/3 amount of total saponin. Especially, the saponin content of leaves decreased according to the sequential collection days, in which the similar results were observed from the flowers and stems of ginseng. The total saponin content of the flowers and stems were 141.09,143.84,139.25,133.47 and 13.32, 9.85, 8.00, 4.65 mg/g, respectively. Among them, the content of Re, Rd and $Rb_2$ in flowers were more than 2/3 while the content of Re, $Rg_1$ and Rd in stems showed more than 9/10 amount of total saponin. The total saponin content of individual leaf were 19.46, 28.56, 58.82 and 169.24 mg/plant, 2.53, 2.76, 5.20 and 12.32 mg/plant in stems, and 14.11, 30.21, 37.60 and 73.41 mg/plant in flowers. Therefore, the total saponin content of aboveground parts in ginseng were leaves > flowers > stems.