• Title/Summary/Keyword: RAG 2

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Effects of Intercrops on Growth and Yield of Paeonia Lactiflora PALLAS. (간작물(間作物)이 작약(芍藥)의 생육(生育) 및 수양(收量)에 미치는 영향(影響))

  • Hwang, Hyung-Baek;Kim, Jae-Chul;Park, So-Deug;Chol, Boo-Sull;Lim, Joo-Rag
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.212-217
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    • 1996
  • This study was carried out to select proper intercrops in peony cultivation because peo­ny is damaged easily by disease and cannot make proper income with it's single crop system, so it needs rational intercropping system to raise it's productivity and income. The results of this study are as follows. The kinds of soil nematodes were Meloidogyne sp, Pratyienchus sp, Aphelenchoides sp, Ditylenchus sp, Xiphinema sp, and damaging dominant nematode was Meloidogyne sp. The density of Meloidogyne sp wa suppressed remarkably in the combination of peony+sesame, and peony + job's tears. This study shows that sesame and job's tear were most resistant crops to the IvIeloi­dogyne sp. The quanity of herbaceous peony was the best in the combination of peony+red pepper. The results of income analysis per lOa for three years shows next conclusion. Peony+red pepper combination and peony+ sesame combination were most effective when it's compared with single cropping of peony (1,490 thousand won). But red pepper was damaged easily by soil nematode, therefore, sesame was the most stable and high yield crop as a intercrop in the cultivation of herbaceous peony.

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Investigation of Helicobacter pylori cagE Locus Diversity on Interleukin-8 Induction in AGS Cell-line (Helicobacter pylori cagE 유전자 다형성이 위상피세포주에서 Interleukin-8 유도능에 미치는 영향)

  • Lee Su-Min;Lee Hak-Sung;Lee In-Suk;Co Yu-Kyung;Han Hae-Won;Oh Jung-Hwan;Park Jae-Myung;Choi Myung-Gyu;Chung In-Sik
    • Journal of Life Science
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    • v.14 no.6 s.67
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    • pp.906-912
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    • 2004
  • Helicobacter pylori infection is highly prevalent, as high as 2/3 of whole population infected, in Korea. H. pylori infection initiates inflammation by induction of interleukin-8 through type IV secretion of CagA. It was recently suggested that induction failure of IL-8 is not associated with defect in cag PAI but associated with cagE locus diversity. This study was designed to investigate ability of 11-8 in-duction according to sequence variation within the cagE gene, cagA TP motifs and vacA m-types in vitro study using AGS cell-line, and to evaluate its association with different clinical outcome. Seventy-four H. pylori stains were isolated from 23 patients with gastric cancer (Ca), 24 subjects with gastritis (G) and 27 patients with duodenal ulcer (Du) in Kangnam St. Mary's Hospital, Seoul, Korea. cagE gene diversity was confirmed by the PCR-RFLP methods with MboI/NlaIII and tyrosine phosphate motifs (TPMs) of cagA was determined TPM-A and C by using DdeI/Tsp5091 restriction enzyme and TPM-B was determend by Real time PCR the method of Owen et al. and IL-8 was measured by ELISA assay. IL-8 activity was positively detected in 59 among 74 strains $(79.7\%)$. IL-8 secretion was significantly increased in MboI A and MboI B type compared to MboI C type and in MboI/NlaIII A-C and B-C type than C-C type. 1L-8 activity was not associated with either the number or composition of cagA tyrosine phosphorylation motifs and vacA m-type. There was no significant difference in IL-8 activity among patient groups. cagE gene diversity is thought to be mainly associated with the induction of IL-8 in H. pylori infection.