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http://dx.doi.org/10.5352/JLS.2004.14.6.906

Investigation of Helicobacter pylori cagE Locus Diversity on Interleukin-8 Induction in AGS Cell-line  

Lee Su-Min (Catholic Research Institute of Medical Science, The Catholic University of Korea)
Lee Hak-Sung (Catholic Research Institute of Medical Science, The Catholic University of Korea)
Lee In-Suk (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Co Yu-Kyung (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Han Hae-Won (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Oh Jung-Hwan (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Park Jae-Myung (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Choi Myung-Gyu (Department of Internal Medicine, Division of Gastroenterology, The Catholic University of Korea)
Chung In-Sik (Catholic Research Institute of Medical Science, The Catholic University of Korea)
Publication Information
Journal of Life Science / v.14, no.6, 2004 , pp. 906-912 More about this Journal
Abstract
Helicobacter pylori infection is highly prevalent, as high as 2/3 of whole population infected, in Korea. H. pylori infection initiates inflammation by induction of interleukin-8 through type IV secretion of CagA. It was recently suggested that induction failure of IL-8 is not associated with defect in cag PAI but associated with cagE locus diversity. This study was designed to investigate ability of 11-8 in-duction according to sequence variation within the cagE gene, cagA TP motifs and vacA m-types in vitro study using AGS cell-line, and to evaluate its association with different clinical outcome. Seventy-four H. pylori stains were isolated from 23 patients with gastric cancer (Ca), 24 subjects with gastritis (G) and 27 patients with duodenal ulcer (Du) in Kangnam St. Mary's Hospital, Seoul, Korea. cagE gene diversity was confirmed by the PCR-RFLP methods with MboI/NlaIII and tyrosine phosphate motifs (TPMs) of cagA was determined TPM-A and C by using DdeI/Tsp5091 restriction enzyme and TPM-B was determend by Real time PCR the method of Owen et al. and IL-8 was measured by ELISA assay. IL-8 activity was positively detected in 59 among 74 strains $(79.7\%)$. IL-8 secretion was significantly increased in MboI A and MboI B type compared to MboI C type and in MboI/NlaIII A-C and B-C type than C-C type. 1L-8 activity was not associated with either the number or composition of cagA tyrosine phosphorylation motifs and vacA m-type. There was no significant difference in IL-8 activity among patient groups. cagE gene diversity is thought to be mainly associated with the induction of IL-8 in H. pylori infection.
Keywords
H. pylori; cagE; IL-8; cagA TPMs;
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