• International Journal of Oral Biology
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• 제39권3호
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• pp.131-136
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• 2014

Porphyromonas gingivalis is one of the most important periodontal pathogens and has been to known to invade various types of cells, including endothelial cells. The present study investigated the mechanisms involved in the internalization of P. gingivalis in human umbilical vein endothelial cells (HUVEC). P. gingivalis internalization was reduced by clathrin and lipid raft inhibitors, as well as a siRNA knockdown of caveolin-1, a principal molecule of lipid raft-related caveolae. The internalization was also reduced by perturbation of actin rearrangement, while microtubule polymerization was not required. Furthermore, we found that Src kinases are critical for the internalization of P. gingivalis into HUVEC, while neither Rho family GTPases nor phosphatidylinositol 3-kinase are required. Taken together, this study indicated that P. gingivalis internalization into endothelial cells involves clathrin and lipid rafts and requires actin rearrangement associated with Src kinase activation.

• Molecules and Cells
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• 제23권1호
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• pp.57-63
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• 2007

Leukotrienes (LTs) are produced by several biosynthetic enzymes including cytosolic phospholipase $A_2$ ($cPLA_2$), 5-lipoxygenase (5-LO), and 5-lipoxygenase activating protein (FLAP) in the perinuclear area. In the present study, we showed that pretreatment with methyl-${\beta}$-cyclodextrin (MβCD), a cholesterol-depleting agent, dramatically reduced the synthesis of LTs in response to A23187 in mast cells. A23187-induced LT synthesis was inhibited by pretreatment with M${\beta}$CD, and this effect was reversed when cholesterol was added. In an approach to identifying the $M{\beta}CD$-sensitive protein(s), we observed that FLAP co-localized with flotillin-1, a lipid raft marker protein, in the lipid raft-rich low-density region of sucrose gradients. In addition, electron microscopic analysis revealed that FLAP co-localized with flotillin-1. Together, these results suggest that FLAP is present in cholesterol-rich lipid raft-like domains and that its localization in these domains is critical for LT synthesis.

• EDISON SW 활용 경진대회 논문집
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• 제5회(2016년)
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• pp.23-31
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• 2016

자유 라디칼을 이용한 RAFT 중합은 성장하는 고분자 반응을 제어할 수 있는 특성이 있어 주목 받고 있는 고분자 합성방법 중 하나이다. 이 반응의 기작은 agent라 불리는 분자를 주축으로 삼아 단량체들을 단계적으로 성장하는 가역적 방법으로 원하지 않는 종결반응으로부터 고분자 라디칼을 보호하는 역할을 수행한다. 보호의 근본적인 원인은 중간체 상태에서의 안정화 정도와 관련이 있으나 안정해진 만큼 반응속도가 느려지는 지연효과가 발생한다. 지연효과를 유도하는 원인은 많은 논란이 있었으며 그 중 하나로 agent에 존재하는 Z 치환기의 영향을 원인으로 지목하고 있다. 본 연구는 Z 치환기의 변화에 따른 안정화 정도를 파악하기 위하여 RAFT agent로 주로 이용하는 것 중 두 개의 황이 있는 dithioester와 xanthate를 WxMacMolplt 7.3.2를 이용하여 propagation 초기 단계를 구현한 후 GAMESS2 프로그램을 이용하여 양자화학적 계산을 수행하였다. 계산결과 안정화 에너지와 경계 궤도함수에서는 phenyl기가 있을 때 공명효과에 의하여 안정화가 이루어졌으며 또한 propyl benzyl에서도 늘어난 알킬 사슬의 donating effect로 인한 안정화 영향의 범위를 발견하였다. PES 기법을 통해 두 methyl 단량체를 움직이면서 반응하는 동안의 에너지 변화를 알아보았으며 그 결과 dithioester는 Z 치환기의 변화에 더 많이 의존한다는 것을 확인하였다. 본 연구를 종합해본 결과 phenyl을 제외한 aryl기가 있는 dithioester는 낮은 addition 퍼텐셜과 안정화 에너지를 가질 수 있을 것이고 지연효과를 줄일 수 있을 것으로 기대된다.

• 한국지반공학회논문집
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• 제29권6호
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• pp.19-31
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• 2013

기존의 설계에서는 상부구조물의 거동은 하부기초 및 지반을 고정단 조건으로 가정하여 분석하였고, 하부기초의 설계는 상부구조물의 하중조건만 고려하였다. 이는 상부구조물 및 기초의 부재력과 침하량을 과다하게 판단할 가능성이 있다. 본 연구에서는, 상부구조물과 말뚝지지 전면기초의 상호작용을 고려한 상호작용 해석기법을 제안하였으며, 전체 구조물의 거동을 파악하기 위한 상호작용해석기법의 적용성을 고찰하였다. 일련의 수치해석을 통하여 상호작용해석과 일체해석의 결과를 비교한 결과, 본 설계기법으로 산정한 말뚝지지 전면기초의 침하거동 및 휨모멘트가 유한요소해석과 유사하게 나타났다. 또한 상부구조물과 하부기초의 상호작용을 고려하여 전체구조물의 거동을 비교적 정확히 산정하는 것을 알 수 있었다. 이러한 검증을 토대로 본 해석기법이 실제 상부구조물-말뚝지지 전면기초설계에 적용 가능할 것으로 판단된다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2002년도 기초기술학술발표회
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• pp.77-89
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• 2002

To analyze a bearing capacity for pile groups, a number of model tests have been done and theoretical methods studied. In the case of design of group pile bearing capacity is calculated with only pile capacity. But uncertainty of bearing capacity and behavior of foundation cap(raft) leads to conservative design ignoring bearing effects of foundation cap. In the case of considering bearing capacity of foundation cap, the simple sum of bearing capacity of foundation cap and pile groups cannot be the bearing capacity of total foundation system. Since cap-pile-soil interaction affects the behavior of pile groups. Thus, understanding cap-pile-soil interaction is very important in optimal design. In this paper, the piled raft behavior is studied through model tests of 2$\times$2, 2$\times$3, 3$\times$3 pile group. Changes of behavior of pile group foundation by touching effects of foundation cap with soil are studied. Also changes of spacing between piles. Foundation cap is made of rigid steel plate and piles are made steel pipes. From this model tests, the changes of behavior changes of pile groups by touching effects of foundation cap with soil are studied.

• 한국컴퓨터정보학회논문지
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• 제26권12호
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• pp.99-104
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• 2021

블록체인 기술은 분산 네트워크에 연결된 다수의 노드 간 원장을 공유하는 분산 원장기술이다. 기존 중앙 서버를 통하여 관리되던 데이터들이 블록체인을 통하여 관리되며 거래 데이터에 대한 투명성과 정확성 및 무결성이 높아지며 블록체인을 통한 데이터관리의 필요성이 높아지고 있다. 본 논문에서는 금융업에서 신뢰 기반의 기관 간 신뢰 기반의 데이터 공유가 필요하다는 점을 인지하고 블록체인을 통한 데이터관리 효율성 증대를 높이는 방법으로 프라이빗 블록체인 합의 알고리즘인 Raft의 리더 노드 선출과정의 처리 과정을 수정한 합의 알고리즘을 제시하였다. 본 논문에서 제시한 수정 합의 알고리즘과 일반적인 Raft 합의 알고리즘에 대하여 트랜잭션 처리시간을 기준으로 성능을 비교·분석하였으며 제안한 합의 알고리즘 적용을 통하여 합의 과정의 효율성이 높아짐을 확인하였다.

• Geomechanics and Engineering
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• 제32권4호
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• pp.427-443
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• 2023

Across the globe, rapid urbanization demands the construction of basements for car parking and sub way station within the vicinity of high-rise buildings supported on piled raft foundations. As a consequence, ground movements caused by such excavations could interfere with the serviceability of the building and the piled raft as well. Hence, the prediction of the building responses to the adjacent excavations is of utmost importance. This study used three-dimensional numerical modelling to capture the effects of twin excavations (final depth of each excavation, H_e=24 m) on a 20-storey building resting on (4×4) piled raft. Because the considered structure, pile foundation, and soil deposit are three-dimensional in nature, the adopted three-dimensional numerical modelling can provide a more realistic simulation to capture responses of the system. The hypoplastic constitutive model was used to capture soil behaviour. The concrete damaged plasticity (CDP) model was used to capture the cracking behaviour in the concrete beams, columns and piles. The computed results revealed that the first excavation- induced substantial differential settlement (i.e., tilting) in the adjacent high-rise building while second excavation caused the building tilt back with smaller rate. As a result, the building remains tilted towards the first excavation with final value of tilting of 0.28%. Consequently, the most severe tensile cracking damage at the bottom of two middle columns. At the end of twin excavations, the building load resisted by the raft reduced to half of that the load before the excavations. The reduced load transferred to the piles resulting in increment of the axial load along the entire length of piles.

• Journal of Photoscience
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• 제9권2호
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• pp.275-277
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• 2002

We have recently found that a detergent-resistant raft like membrane (DRM) can be prepared from bovine rod outer segment membranes as a low-density buoyant fraction in sucrose density gradient ultracentrifugation. G protein (transducin) and its effector enzyme (phosphodiesterase: PDE) drastically change their affinities to DRM in the process of phototransduction. We report here that the recruitment of transducin and/or $^2$PDE to DRM has close relationship with their states in signal transduction. Active T$\alpha$/PDE-complex has a high affinity to DRM, whereas inactive transducin, or inactive PDE are excluded from DRM. Active T$\alpha$/PDE-complex seems to bind to a GTPase activating protein (GRS9) in multi- protein complexes localized on DRM. Physiological significance of the multi-protein complex on the raft-like membrane in vertebrate phototransduction would be discussed.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 세계 도시지반공학 심포지엄
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• pp.967-975
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• 2009

In order to control differential settlement and to secure the safety of super structure on a high rock embankment the designed static compaction is changed with dynamic compaction and piled raft method. The parameters for dynamic compaction design are obtained from a pilot test. In addition, numerical analyses are also carried out to figure out the length and quantity of piled raft that can restraint the differential settlement within allowance range.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.319-319
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• 2006

RAFT polymerization can produce under controlled conditions polymer chains incorporating well-defined chain ends. By designing a simple way of producing functional RAFT agents, a variety of ${\Box}-end$ groups was successfully introduced onto hydrophilic polymer chains. The ${\Box}-end$ group being a thiocarbonyl thio function was used as efficient chain transfer agent in dispersion or emulsion polymerization to produce original functional latex particles.