• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.117-122
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• 2006

Comparative studies on medium supply in bioreactors (raft, immersion and ebb and flood) have revealed that multiplication and growth of Alocasia Amazonica were greatest in the raft system, while lowest in ebb and flood system. In the raft system, the basal part of the shoots was continuously in contact with medium, which enabled a constant uptake of nutrients as well as aeration to the explants. The number and the size of leaf stomata were higher in the raft system compared with immersion and ebb&flood system. In the immersion system, plantlets were deformed and epidermal cells in leaves were irregular with a large intercellular space. The results suggested that the medium supply should be controlled properly to maintain normal and healthy plantlets during liquid cultures in bioreactors Which affects morphology and physiology Of the plantlets.

• Journal of the Korean GEO-environmental Society
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• v.13 no.11
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• pp.43-50
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• 2012

In this research, the value of consolidation index was investigated. The range of the investigated standard deviation was analyzed and the deviation based settlement was calculated. Also, the compression index, which is the effect of the uncertainty in the ground was analyzed using the flimsy ground construction method. The settlement behavior in each embankment compaction stage was analyzed by applying the precompression load method, drainage expediting method, and displacement method through numerical analysis. In addition to the above, the settlement behavior was studied by analyzing the Piled Raft method which is stable for long term settlement. As a result, the final settlement amount based on average analysis results was that the settlement based on each of the average interpretation value, mean value of the maximum and minimum value and average compression index was different. The result of the comparison shows the difference in variation coefficient by the difference in time. Amongst them, the Piled Raft method shows the most consistent variation coefficient regardless of time and it also was least affected by the compression index of uncertainty.

• Journal of the Korean Geotechnical Society
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• v.28 no.4
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• pp.67-78
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• 2012

A three-dimensional approximate computer-based method, YSPR (Yonsei Piled Raft), was developed for analysis of behavior of piled raft foundations. The raft was modeled as a flat shell element having 6 degrees of freedom at each node and the pile was modeled as a beam-column element. The behaviors of pile head and soil were controlled by using $6{\times}6$ stiffness matrix. To model the non-linear behavior, the soil-structure interaction between soil and pile was modeled by using nonlinear load-transfer curves (t-z, q-z and p-y curves). Comparison with previous model and FEM analysis showed that YSPR gave similar load-displacement behaviors. Comparison with field measurement also indicated that YSPR gave a reasonable result. It was concluded that YSPR could be effectively used in analysis and design of piled raft foundations.

• Geomechanics and Engineering
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• v.35 no.6
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• pp.617-626
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• 2023

In this study, three-dimensional numerical parametric study was conducted to explore deformation mechanisms of grouped piled-raft-foundation due to lateral load in clays. Effects of load intensity, loading angle, soil stiffness, pile diameter, pile spacing and pile length on foundation deformations were explored. It is found that the smallest and largest movements of pile foundation are induced when the loading angles are 0° and 30°~60°, respectively. By increasing loading angle from 0° to 30°~60°, the resultant horizontal movements and settlements increase by up to 20.0% and 57.1%, respectively. Since connection beams can substantially increase integrity of four piled raft foundation, resultant horizontal movements, settlements and bending moments induced in the piled raft foundation decrease by up to 54.0%, 8.8% and 46.3%, respectively. By increasing soil stiffness five times, resultant horizontal movements and settlements of pile foundation decrease by up to 61.7% and 13.0%, respectively. It is indicated that effects of connection beam and soil stiffness on settlements of pile foundation are relatively small. When pile diameter is less than 1.4 m, deformations of piled raft foundation decrease substantially as a reduction in the pile diameter. Two dimensional groups are proposed to develop calculation charts of horizontal movements and settlements of pile foundation. The proposed calculation charts can directly estimate movements of piled raft foundation under arbitrary loading, ground and pile conditions.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.56-63
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• 2007

Insulin stimulates the fusion of intracellular vesicles containing glucose transporter 4 (GLUT4) with plasma membrane in adipocytes and muscle cells. Here we show that adipocyte differentiation results in enhanced insulin sensitivity of glucose uptake. On the other hand, glucose uptake in response to platelet-derived growth factor (PDGF) stimulation was markedly reduced by adipocyte differentiation. Expression level of insulin receptor and caveolin-1 was dramatically increased during adipocyte differentiation. Adipocyte differentiation caused :ilightly enhanced activation of acutely transforming retrovirus AKT8 in rodent T cell lymphoma (Akt) by insulin stimulation. However, activation of Akt by PDGF stimulation was largely reduced. Activation of ERK was not detected in both fibroblasts and adipocytes after stimulation with insulin. PDGF-dependent activation of ERK was reduced by adipocyte differentiation. Insulin-dependent glucose uptake was abrogated by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, in both fibroblasts and adipocytes. Also disassembly of caveolae structure by $methyl-\beta-cyclodextrin$ caused impairment of Akt activation and glucose uptake. Finally, insulin receptor, Akt, SH2-domain-containing inositol 5-phosphatase 2 (SHIP2), and regulatory subunit of PI3K are localized at lipid raft domain and the translocation was facilitated upon insulin stimulation. Given these results, we suggest that lipid raft provide proper site for insulin action for glucose uptake.

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.307-308
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• 2007

• Proceedings of KOSOMES biannual meeting
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• 2017.04a
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• pp.173-173
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• 2017

• Journal of Life Science
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• v.32 no.2
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• pp.148-154
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• 2022

Focal adhesion kinase (FAK) is known to regulate cell adhesion, migration, and mechanotransduction in focal adhesions (FAs). However, studies on how FAK activity is regulated in the plasma membrane microdomains according to the composition of extracellular matrix (ECM) proteins are still lacking. A genetically encoded fluorescence resonance energy transfer (FRET)-based biosensor can provide useful information on the activity of intracellular signals with high spatiotemporal resolution. In this study, we analyzed the FAK activities in lipid raft (detergent-resistant membrane) and non-lipid raft (non-detergent-resistant membrane) microdomains using FRET-based membrane targeting FAK biosensors (FAK-Lyn and FAK-KRas biosensors) under four different ECM protein compositions: glass, type 1 collagen, fibronectin, and laminin. Interestingly, FAK activity in response to laminin in a lipid raft microdomain was lower than that in other ECM conditions. Cells subjected to fibronectin showed higher FAK activity in a lipid raft microdomain than that in a non-lipid raft microdomain. Therefore, this study demonstrates that the FAK activity can be distinctively regulated according to the ECM type and the environment of the plasma membrane microdomains.

• International Journal of High-Rise Buildings
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• v.4 no.4
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• pp.271-281
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• 2015

This paper introduces detailed three-dimensional numerical analyses on a bored pile foundation for a high-rise building. A static load test was performed on a test pile and a numerical model of a single pile, which was calibrated by comparing it with the test result. The detailed numerical analysis was then conducted on the entire high-rise building foundation. Further study focused on soil pressures under the base slab of a piled raft foundation. Total seven cases with different pile numbers and raft-soil contact conditions were investigated. The design criteria of a foundation, especially settlement requirement were satisfied even for the cases with fewer piles under considerable soil pressure beneath the base slab. The bending moment for the structural design of the base slab was reduced by incorporating soil pressures beneath the base slab along with bored piles. Through the comparative studies, it was found that a more efficient design can be achieved by considering the soil pressure beneath the slab.

• International Journal of Oral Biology
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• v.39 no.3
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• pp.131-136
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• 2014

Porphyromonas gingivalis is one of the most important periodontal pathogens and has been to known to invade various types of cells, including endothelial cells. The present study investigated the mechanisms involved in the internalization of P. gingivalis in human umbilical vein endothelial cells (HUVEC). P. gingivalis internalization was reduced by clathrin and lipid raft inhibitors, as well as a siRNA knockdown of caveolin-1, a principal molecule of lipid raft-related caveolae. The internalization was also reduced by perturbation of actin rearrangement, while microtubule polymerization was not required. Furthermore, we found that Src kinases are critical for the internalization of P. gingivalis into HUVEC, while neither Rho family GTPases nor phosphatidylinositol 3-kinase are required. Taken together, this study indicated that P. gingivalis internalization into endothelial cells involves clathrin and lipid rafts and requires actin rearrangement associated with Src kinase activation.