• Title/Summary/Keyword: R9

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Mutagenesis of Slow Growing Rhizobium japonicum by Transposon Tn5 (Transposon Tn5를 이용한 Slow growing Rhizobium japonicum의 돌연변이 유도)

  • Kim, Sung-Hoon;Rhee, Yoon;Sun, Dae-Kyu;Yoo, Ick-Dong
    • Korean Journal of Microbiology
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    • v.26 no.4
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    • pp.305-311
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    • 1988
  • The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168 $spr^{r}$ chromosome at the frequency of $1.0\times 10^{-5}-5.0\times 10^{-7}$ and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin ($50{\mu}$g/ml) and spectinomycin ($100{\mu}$g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.

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Influence of $Ar^+$ ion Bombardment on the Chemical States of ${SrBi_2}{Ta_2}{O_9}$ Thin Films Fabricated by Metal-Organic Decomposition ($Ar^+$이온 충격이 MOD 법에 의해 제조된 ${SrBi_2}{Ta_2}{O_9}$박막의 화학 상태에 미치는 영향)

  • Park, Yoon-Baek;Cho, Kwang-Jun;Lee, Moon-Keun;Heo, Sung;Lee, Tae-Kwon;Kim, Ho-Joung;Min, Kyung-Youl;Lee, Sun-Young;Kim, Yil-Wook
    • Journal of the Korean Ceramic Society
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    • v.37 no.11
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    • pp.1084-1090
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    • 2000
  • (Bi$_2$O$_2$)$^{2+}$층 사이에 두 개의 Ta-O 팔면체로 연결된 Bi 계의 층상 페로브스카이트 구조인 SrBi$_2$Ta$_2$O$_{9}$ (SBT) 박막을 XPS를 이용하여 깊이별 화학 상태 변화를 분석하였다. 아르곤 이온으로 SBT 박막을 식각하면, SBT 박막의 각 구성물들은 가속 Ar$^{+}$ 이온의 에너지에 따라 변화한다. SBT 각 구성물 중 Sr 3d의 화학 상태는 Ar$^{+}$ 이온의 에너지변화에 따라 근소하게 변화한다. 반면에, Ta 4f와 Bi 4f의 화학 상태 변화는 인가되는 Ar$^{+}$ 이온 에너지에 확실하게 의존한다. 특히, Bi 4f는 Sr과 Ta에 비해 낮은 Ar$^{+}$ 이온 에너지에서도 Bi-O의 화학 상태가 금속 Bi 화학 상태로 현저하게 변화한다. 이러한 SBT 박막의 화학 상태 변화는 산호 원자의 선택적인 식각 때문에 발생하며 선택적인 식각은 SBT 박막 내에서 각 구성물과 산소간의 질량 차이와 각 구성물의 열적 안정성에 의존함을 알 수 있다.

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Study on the Performance of Heat Pump Using Non-azeotropic Refrigerant Mixtures R-22+R-114 (비공비혼합냉매 R-22+R-114를 이용한 열펌프의 성능에 관한 연구)

  • 박기원;구학근;오후규
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.17 no.9
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    • pp.2131-2137
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    • 1993
  • This study, examines the performance and the heat pump cycle systematizing characteristics for non-azeotropic refrigerant systems. In order to conduct such an examination, the cycle characteristics of heat pumps for pure R-22, R-114, and their mixtures were experimentally investigated. The results show that cooling/heating capacities for the mixtures was more suited at the evaporating temperature of $5^{\circ}C$ than that of $0^{\circ}C$, $-5^{\circ}C$, and $-10^{\circ}C$. The C.O.P of the 50 wt% mixtures was considerably higher than for pure R-22, and the compression power of the 25 wt% was as much as 60% lower than that of pure R-22. Even small fractional mixture variations can lead to significant changes in the characteristics of the heat pump cycle. This experiment verified the importance of accurate weight fractions of refrigerant mixtures.

Prefetch R-tree: A Disk and Cache Optimized Multidimensional Index Structure (Prefetch R-tree: 디스크와 CPU 캐시에 최적화된 다차원 색인 구조)

  • Park Myung-Sun
    • The KIPS Transactions:PartD
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    • v.13D no.4 s.107
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    • pp.463-476
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    • 2006
  • R-trees have been traditionally optimized for the I/O performance with the disk page as the tree node. Recently, researchers have proposed cache-conscious variations of R-trees optimized for the CPU cache performance in main memory environments, where the node size is several cache lines wide and more entries are packed in a node by compressing MBR keys. However, because there is a big difference between the node sizes of two types of R-trees, disk-optimized R-trees show poor cache performance while cache-optimized R-trees exhibit poor disk performance. In this paper, we propose a cache and disk optimized R-tree, called the PR-tree (Prefetching R-tree). For the cache performance, the node size of the PR-tree is wider than a cache line, and the prefetch instruction is used to reduce the number of cache misses. For the I/O performance, the nodes of the PR-tree are fitted into one disk page. We represent the detailed analysis of cache misses for range queries, and enumerate all the reasonable in-page leaf and nonleaf node sizes, and heights of in-page trees to figure out tree parameters for best cache and I/O performance. The PR-tree that we propose achieves better cache performance than the disk-optimized R-tree: a factor of 3.5-15.1 improvement for one-by-one insertions, 6.5-15.1 improvement for deletions, 1.3-1.9 improvement for range queries, and 2.7-9.7 improvement for k-nearest neighbor queries. All experimental results do not show notable declines of the I/O performance.

Evaluation of Coated Tube CA19-9 IRMA kit (Coated tube를 사용한 CA19-9 측정용 IRMA 시약의 평가)

  • Lee, Hyun-Ju;Jang, Hyun-Young;Shin, Sun-Young;Kim, Hee-Sun;Kim, Tae-Hoon;Lee, Ho-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.2
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    • pp.208-211
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    • 2010
  • Purpose: $TFB^{(R)}$ CA19-9 IRMA kit uses beads coated with CA19-9 antibody. However, this mathod can not use automated equipment, and requires a long time test. Recently, CA19-9 IRMA kit developed by $TFB^{(R)}$ is coated with CA19-9 antibody to the polystyrene test tube and reaction at room temperature, and also reduced test time. This study evaluated the performance of a newly developed $TFB^{(R)}$ CA19-9 IRMA kit. Materials and Methods: This study were measured by using 56 patients sample of Boramae Medical Center and Seoul National University Hospital. We evaluated intra-and inter-assay precision, recovery rate, linearity, sensitivity and high dose hook effect of coated tube CA19-9 IRMA kit developed by $TFB^{(R)}$. The values of CA19-9 measured by $TFB^{(R)}$ bead kit were compared with those measured by $TFB^{(R)}$ coated tube kit. Results: ntra-assay coefficients of variation on three different levels were 4.1%, 4.0% and 4.2%. Inter-assay coefficients of variation were 7.6%, 4.3% and 7.8%. Recovery tests on all three different levels showed within $100{\pm}10%$. Linearity was good and sensitivity was 0.3 U/mL. High dose hook effect is not observed. There was strong correlation between bead kit and coated tube kit by $TFB^{(R)}$ CA19-9 IRMA kit. (y=0.9185x-0.953, $R^2$=0.9779) Conclusion: Coated tube CA19-9 IRMA kit developed by $TFB^{(R)}$ showed satisfactory precision, recovery rate, linearity, sensitivity and high dose hook effect.

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Condensation Heat Transfer Coefficients of R245fa on a Plain Tube (수평관에서 R245fa의 응축 열전달계수)

  • Shim, Yun-Bo;Park, Ki-Jung;Jung, Dong-Soo;Kim, Jong-Seong
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.8
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    • pp.555-562
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    • 2007
  • In this study, condensation heat transfer coefficients (HTCs) of R22, R134a, R245fa and R123 are measured on a horizontal plain tube. All data are taken at the vapor temperature of $39^{\circ}C$ with a wall subcooling temperature $3-8^{\circ}C$. Test results show the HTCs of newly developed alternative low vapor pressure refrigerant, R245fa, on a plain tube are 9.5% higher than those of R123 while they are 3.3% and 5.6% lower than those of R134a and R22 respectively. Nusselt's prediction equation for a plain tube underpredicts the data by 13.7% for all refrigerants while a modified equation yielded 5.9% deviation against all data. From the view point of environmental safety and condensation heat transfer, R245fa is a long term good candidate to replace R123 used in centrifugal chillers.

Condensation heat transfer of R407C and R410A in a horizontal smooth tube (R407C 및 R410A의 수평원관내 응축열전달)

  • 서정현;김민수
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.11 no.5
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    • pp.633-641
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    • 1999
  • Experiments were carried out to investigate the condensation heat transfer characteristics for R22 and its alternatives, R407C (R32/125/134a, 23/25/52wt%) and R410A (R32/125, 50/50wt%). A concentric tube heat exchanger was made to conduct condensation heat transfer tests. Mass flux and saturation temperature of refrigerants at the test section inlet were varied to get the corresponding heat transfer coefficients. Serial and parallel input of secondary fluid (water) were applied to the test subsections. Compared with existing correlations of condensation heat transfer, experimental heat transfer coefficients obtained in this study were generally higher than the predicted values, and mean absolute deviations from several correlations were shown. Wall subcooling was introduced to get a new correlation for condensation heat transfer coefficients by modifying Shah's equation. The RMS deviation of the measured heat transfer coefficients from the new correlation in this study for R22 is 9.9% and that for R407C and R410A are 10.2% and 14.6%, respectively.

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An Empirical Analysis on the Spillover Effects of R&D Investment in the ICT industry (ICT 산업의 R&D 투자가 타 산업에 미치는 파급효과 측정)

  • Jung, Woo-Jin;Kim, Hyunsuk;Cho, Shin
    • Knowledge Management Research
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    • v.20 no.1
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    • pp.27-43
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    • 2019
  • This paper examines spillover effects of R&D investment in the ICT industry to other industries by analyzing panel data composed of 6 manufacturing industries for the period of 1983~2011. Major findings are as follows; (1) The increase of R&D investment in ICT industry has a significantly positive relationship with the increase in the value-added of the remaining 5 industries with 3~10 year lags, depending on the source of R&D funds and R&D stage. (2) More specifically, public R&D shows the spillover effects during 3~7-year lag periods, whereas private R&D started to exert spillover effects from 9-year lag. The result implies that the public R&D appropriately targets projects with more spillover effects. (3) Basic research, applied research, and development showed the spillover effects for the period of 9~10, 7~10, and 10-year lag, respectively. (4) The R&D investment in 5 other industries such as machinery, chemicals, and materials does not have positive spillover effects to the remaining industries. Few studies have been done to analyze the R&D spillover effects to other industries, with certain limitations. This study contributes to the existing literature by analyzing the spillover effects of the different R&D funds and R&D stages, and by considering various time-lag effects.

Comparison of the Results of Multistix$^{(R)}$SG and Comber-9-Test$^{(R)}$ RL Urine Dipstick Assay (Multistix$^{(R)}$-SG와 Comgur-9-test$^{(R)}$RL에 의한 요시험지봉검사 성적의 비교)

  • Kim, Dae-Chul;Kim, Kyung-Dong;Jung, Bo-Chan;Kim, Chung-Sook;Cho, Kil-Ho
    • Journal of Yeungnam Medical Science
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    • v.8 no.1
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    • pp.42-52
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    • 1991
  • Two types of urine dipstick assays, Multistix-SG and Combur-9-test RL, were compared for compatibility, accuracy, specificity and predictive values of a positive and negative test in 501 patients urine and artificially prepared specimen. We found that the results of semiquantitative tests of Multistix-SG and Combur-9-Test RL performed were statistically similar in patients specimen. The urinary leukocyte estrase tests of Combur-9-Test RL assays compared with urine sediment microscopy in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative test 83.7%, 48.1%, 90.3%, 47.4% and 90.1%, respectively. The urinar nitrite tests of Comber-9-Test RL assays compared with urine culture tests, in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative tests were 90.3%, 19.4%, 84.7%, 53.8% and 94.1%, respectively. For the urinary protein, the sulfosalicylic acid method was the most sensitive test for any kinds of protein, and Multistix-SG appeared more sensitive than Compur-9-Test RL for the albuminuria. For the urinary bilirubin and glusose, two dipstick assays were similar in their diganostic efficiency. Finally in the urinary occult blood tests, Combur-9-Test RL assays was more sensitive than Multistix-SG.

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Mass Loss and Changes of Mineral Nutrients during the Decomposition of Mushrooms, Russula alboareolata and Lactarius violascens

  • Mun, Hyeong-Tae
    • Animal cells and systems
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    • v.4 no.1
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    • pp.51-55
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    • 2000
  • Mass loss and changes of mineral nutrients during the decomposition of mushrooms, Russula alboareolata and Lactarius violascens, were investigated for 7d from June 29 to July 5 in 1999 in an oak stand in Kongju, Korea. At 7d after installation of litterbags, the remaining mass of R. alboareolata and L. violaxcens was 9.4% and 25.9%, respectively. The mass loss rate of R. alboareolata was significantly higher than that of L. violascens. Concentration of N, P, K, Ca and Mg of R. alboareolata and L. violascens were 37.7 mg/g, 0.97mg/g, 38.25 mg/g, 0.04 mg/g, and 0.75 mg/g for R. alboareolata and 45.7 mg/g, 1.31mg/g, 24.0 mg/g, 0.06 mg/g, and 0.80 mg/g for L. violascens, respectively. Concentrations of nutrients in R. alboareolata and L. violascens were much higher than those in the surrounding leaf litter. N, P, Ca and Mg concentrations in the decomposing mushroom tissue were higher during the experimental period in both species than initial concentrations. Potassium increased during the first 3 d and then decreased in both species. Potassium contents in the mushroom were much greater than those of Ca and Mg. Except for Ca, there was no immobilization period in all the nutrients during decomposition. At 7 d after installation of litterbags, the remaining N, P, K, Ca and Mg of R. alboareolata and L. violascens were 9.8%, 8.9%, 2.7%, 47.7%, and 14.8% of the initial contents for R. alboareolata and 28.2%,30.5%, 19.6%, 199.9%, and 02.1% for L. violascens, respectively. Nutrients could be relocated spatially during the formation and decomposition of the Basidiomycetes fruiting body.

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