• 한국발생생물학회지:발생과생식
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• 제22권1호
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• pp.39-53
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• 2018

The purpose of this study is to investigate the early life history of the Rhodeus fish, Rhodeus uyekii and R. ocellatus, in the Nakdong River to use as the preliminary data for the systematic study. The embryos used in the study were fertilized eggs (embryo) and larvae after artificial fertilization. The long diameter of the eggs of the R. uyekii was 3.39-3.97 mm (average $3.68{\pm}0.41mm$, n=30) and the short diameter was 1.36-1.55 mm (average $1.45{\pm}0.13mm$, n=30). The long diameter of the eggs of the R. ocellatus was 2.53-2.71 mm (average $2.62{\pm}0.12mm$, n=30) and the short diameter was 1.47-1.60 mm (average $1.53{\pm}0.09mm$, n=30). Hatching time was 48 hours for the R. uyekii and 50 hours for the R. ocellatus given that the average water temperature was $21.5^{\circ}C$. The hatched larvae were 4.95-5.00 mm (average $4.98{\pm}0.04mm$, n=5) for the R. uyekii and the total length was 3.66-3.69 mm (average $3.67{\pm}0.02mm$, n=5) for the R. ocellatus. R. uyekii was found to be 15.5-15.8 mm at total length (average $15.6{\pm}0.21mm$, n=5) on the 56 days after hatching with the number of dorsal fins being ⅲ-9, anal fins ⅲ-10, ventral fins ⅲ-5. The R. ocellatus was found to be 15.8-16.0 mm (average $15.9{\pm}0.14mm$, n=5) at total length on the 58 days with the number of dorsal fins being ⅲ-11, anal fins ⅲ-12 and ventral fins ⅲ-5 where the number of all fin stalks reached maximum.

• 한국수산과학회지
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• 제52권4호
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• pp.408-417
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• 2019

This study was conducted to identify taxonomic differences in the characteristics of Rhodeus uyekii and Rhodeus ocellatus during their initial life history via an interspecific hybridization experiment. Hybrids were compared to their parent species, and the findings were used to inform developmental research in commercially useful aquarium fish. The hatching rates of the cross-bred eggs were 75.9% for cross UO (R. uyekii ♀${\times}$R. ocellatus♂) and 71.9% for cross OU (R. ocellatus♀${\times}$R. uyekii♂), which did not differ greatly from the hatching rates of the normal cross-bred group. Backcross experiments resulted in 100% egg mortality during development. Newly hatched larvae of the original hybrid crosses were similar to those of the maternal line, and the color of the egg yolk was similar to that of the paternal line; therefore, the respective traits of the interspecific parents were identifiable within the cross-bred offspring.

• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.33-41
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• 2014

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

• 한국어류학회지
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• 제9권1호
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• pp.130-140
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• 1997

한국산(韓國産) 납자루아과(亞科)에 속하는 각시붕어(Rhodues uyekii) 골격(骨格)의 주요 형태적 특징을 기재하고자 조사를 수행하였다. 본 조사에서 R. uyekii의 두골(頭骨), 척추골(脊椎骨), 지느러미 골격(骨格)을 조사였던 바, 두개부(頭蓋部)에 fontanell이 없으며, 상사골(上篩骨)의 전단에 돌기가 없고, 유리신경간극(遊離神經間棘)이 넓은 판상(板狀)이고, 상후두골(上後頭骨)은 높은 삼각형 형태의 돌기를 갖고 있으며, 제4 안하골(眼下骨)이 매우 축소 되어 있고, 미설골(尾舌骨)의 선단(先端)이 2분지(分枝)되고 수평판(水平板)과 수직판(垂直板)의 후방(後方)이 넓어지며 후연(後緣)이 돌출되는점, 견대부(肩帶部)에는 오훼골(烏喙骨) 구멍이 존재하지 않았고, 미기추전추체에 미신경골(尾神經骨)이 나타나지 않는 특징을 보였다.

• 한국어류학회지
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• 제18권4호
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• pp.339-346
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• 2006

우리나라 고유종인 각시붕어 Rhodeus uyekii의 이용을 위한 연구의 일환으로 동속의 유사종으로 알려진 떡납줄갱이 R. notatus와 상호 교잡을 실시하였다. 이들 두 종은 난의 형태와 크기에 있어 뚜렷한 차이를 보여 잘 구분되었으며, 초기 자어의 난황 형태에 있어서도 각시붕어는 콩깍지모양인 반면 떡납줄갱이는 닻 형이어서 잘 구분되었다. 한편 종간 상호 교잡은 수정율과 부화율에 있어서 정상 교배군과 큰 차이가 없을 정도로 자유로워 UN형이 부화율 71.6%이었으며, NU형이 부화율 97.5%에 달했다. 교잡체의 부화 자어 형태는 UN형에 있어서 극 소수이지만 UU형이 출현하여 주목되었으나 대부분 NN형이 우세한 중간형태이었고, NU형에 있어서는 NN형이 전혀 출현하지 않았고 모두 UU형이 우세한 중간형태로 부계 형질이 우선하는 것으로 사료되었다. 이들 교잡체는 현재 사육중으로 추후 외부형태, 성별 구분, 생식소 기능, 핵형 등을 분석할 예정이다.

• 한국양식학회지
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• 제22권1호
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• pp.79-82
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• 2009

한국 고유종인 각시붕어 R. uyekii와 떡납줄갱이 R. notatus로부터 유도된 정교배 및 상반교배 잡종어류의 분자생물학적 동정을 위하여 핵에서 encoding되는 RAG-1 유전자의 염기서열 분석을 실시하였다. 분석된 863 bp의 염기서열 중 각시붕어와 떡납줄갱이 사이에는 총 13개의 위치에서 염기서열 변이가 탐색되었다. 잡종어류의 RAG-1 유전자 염기서열을 분석한 결과 모계와 부계의 염기서열 차이를 보인 13개의 변이 부분에서 부모의 염기서열을 다같이 반영하는 double peaks 패턴을 보였으나 정교매체(UN 유전형)와 상반교배체(NU 유전형) 간의 염기서열 차이는 관찰되지 않았다.

• 한국어류학회지
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• 제24권3호
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• pp.151-159
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• 2012

정교배체인 각시붕어, Rhodeus uyekii (♀)${\times}$떡납줄갱이, R. notatus (♂) 잡종, 그리고 상반교배체인 떡납줄갱이 (♀)${\times}$각시붕어(♂) 잡종을 대상으로 혈액도말을 통한 적혈구 크기, flowcytometry를 통한 세포유전학적인 형질 및 truss dimension과 classical dimension에 의한 외부 계측형질에서의 특성을 부모종간의 차이를 비교하였다. 각시붕어와 떡납줄갱이의 정교배체 및 상반교배체의 계측형질은 일반적으로 이들 유도시 사용된 모계와 부계 종의 중간을 보였다. 정교배체는 14개의 계측형질이 부계의 형질과 유사하였으며 7개의 계측형질이 모계의 형질과 유사하였다. 상반교배체의 경우, 13개의 계측형질이 부계의 형질을, 5개의 계측형질이 모계의 형질과 유사하였다. 정교배체와 상반교배체의 체색소 분포는 중간을 보이나 양친 종을 닮게 나타났다(P<0.05). 적혈구 크기와 DNA 함량은 정교배체와 상반교배체 모두 모계의 적혈구 크기 및 DNA 함량을 따르는 경향이 나타났다(P<0.05). 본 연구에서 파악된 각시붕어와 떡납줄갱이의 잡종 계측형질 및 세포유전학적인 형질은 납자루아과의 관상어 개발시 종간 구별에 유용할 것이다.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.429-433
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• 2011

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (${\tau}_0$). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between ${\tau}_0$ and water temperatures at all temperatures studied (Y = -1.225X + 70.05, $r^2=0.988$, where Y is ${\tau}_0$ and X is temperature).

• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.135-144
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• 2015

Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70 (RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70, HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.251-258
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• 2014

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.